scholarly journals Probing red blood cell mechanics, rheology and dynamics with a two-component multi-scale model

2014 ◽  
Vol 372 (2021) ◽  
pp. 20130389 ◽  
Author(s):  
Xuejin Li ◽  
Zhangli Peng ◽  
Huan Lei ◽  
Ming Dao ◽  
George Em Karniadakis
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Microfluidic Channel ◽  
Scale Model ◽  
Cross Sectional ◽  
Whole Cell ◽  
Multi Scale ◽  
Rbc Membrane ◽  
Two Component

This study is partially motivated by the validation of a new two-component multi-scale cell model we developed recently that treats the lipid bilayer and the cytoskeleton as two distinct components. Here, the whole cell model is validated and compared against several available experiments that examine red blood cell (RBC) mechanics, rheology and dynamics. First, we investigated RBC deformability in a microfluidic channel with a very small cross-sectional area and quantified the mechanical properties of the RBC membrane. Second, we simulated twisting torque cytometry and compared predicted rheological properties of the RBC membrane with experimental measurements. Finally, we modelled the tank-treading (TT) motion of a RBC in a shear flow and explored the effect of channel width variation on the TT frequency. We also investigated the effects of bilayer–cytoskeletal interactions on these experiments and our simulations clearly indicated that they play key roles in the determination of cell membrane mechanical, rheological and dynamical properties. These simulations serve as validation tests and moreover reveal the capabilities and limitations of the new whole cell model.

scholarly journals Red Blood Cell Transfusion in the Emergency Department: An Observational Cross-Sectional Multicenter Study

2021 ◽  
Vol 10 (11) ◽  
pp. 2475
Author(s):  
Olivier Peyrony ◽  
Danaé Gamelon ◽  
Romain Brune ◽  
Anthony Chauvin ◽  
Daniel Aiham Ghazali ◽  
...  
Keyword(s):  
Emergency Department ◽  
Logistic Regression ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Cross Sectional Study ◽  
Red Blood Cell Transfusion ◽  
Cross Sectional ◽  
Acute Bleeding ◽  
History Of ◽  
Rbc Transfusion

Background: We aimed to describe red blood cell (RBC) transfusions in the emergency department (ED) with a particular focus on the hemoglobin (Hb) level thresholds that are used in this setting. Methods: This was a cross-sectional study of 12 EDs including all adult patients that received RBC transfusion in January and February 2018. Descriptive statistics were reported. Logistic regression was performed to assess variables that were independently associated with a pre-transfusion Hb level ≥ 8 g/dL. Results: During the study period, 529 patients received RBC transfusion. The median age was 74 (59–85) years. The patients had a history of cancer or hematological disease in 185 (35.2%) cases. Acute bleeding was observed in the ED for 242 (44.7%) patients, among which 145 (59.9%) were gastrointestinal. Anemia was chronic in 191 (40.2%) cases, mostly due to vitamin or iron deficiency or to malignancy with transfusion support. Pre-transfusion Hb level was 6.9 (6.0–7.8) g/dL. The transfusion motive was not notified in the medical chart in 206 (38.9%) cases. In the multivariable logistic regression, variables that were associated with a higher pre-transfusion Hb level (≥8 g/dL) were a history of coronary artery disease (OR: 2.09; 95% CI: 1.29–3.41), the presence of acute bleeding (OR: 2.44; 95% CI: 1.53–3.94), and older age (OR: 1.02/year; 95% CI: 1.01–1.04). Conclusion: RBC transfusion in the ED was an everyday concern and involved patients with heterogeneous medical situations and severity. Pre-transfusion Hb level was rather restrictive. Almost half of transfusions were provided because of acute bleeding which was associated with a higher Hb threshold.

A Red Blood Cell Model to Estimate the Hemolysis Fingerprint of Cardiovascular Devices

2017 ◽  
Vol 42 (1) ◽  
pp. 58-67 ◽  
Author(s):  
Riccardo Toninato ◽  
Giuseppe Fadda ◽  
Francesca Maria Susin
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Cardiovascular Devices

scholarly journals Altered Levels of Desaturation and ω-6 Fatty Acids in Breast Cancer Patients’ Red Blood Cell Membranes

Metabolites ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 469
Author(s):  
Javier Amézaga ◽  
Gurutze Ugartemendia ◽  
Aitziber Larraioz ◽  
Nerea Bretaña ◽  
Aizpea Iruretagoyena ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Blood Cell ◽  
Cancer Patients ◽  
Red Blood Cell ◽  
Breast Cancer Patients ◽  
Disease Evolution ◽  
Lipidomic Analysis ◽  
Rbc Membrane

Red blood cell (RBC) membrane can reflect fatty acid (FA) contribution from diet and biosynthesis. In cancer, membrane FAs are involved in tumorigenesis and invasiveness, and are indicated as biomarkers to monitor the disease evolution as well as potential targets for therapies and nutritional strategies. The present study provides RBC membrane FA profiles in recently diagnosed breast cancer patients before starting chemotherapy treatment. Patients and controls were recruited, and their dietary habits were collected. FA lipidomic analysis of mature erythrocyte membrane phospholipids in blood samples was performed. Data were adjusted to correct for the effects of diet, body mass index (BMI), and age, revealing that patients showed lower levels of saturated fatty acids (SFA) and higher levels of monounsaturated fatty acid, cis-vaccenic (25%) than controls, with consequent differences in desaturase enzymatic index (∆9 desaturase, –13.1%). In the case of polyunsaturated fatty acids (PUFA), patients had higher values of ω-6 FA (C18:2 (+11.1%); C20:4 (+7.4%)). RBC membrane lipidomic analysis in breast cancer revealed that ω-6 pathways are favored. These results suggest new potential targets for treatments and better nutritional guidelines.

scholarly journals Numerical simulations of blood cell flow in non-Newtonian fluid

2020 ◽  
Vol 306 ◽  
pp. 01006
Author(s):  
Kazuhiro Shitara ◽  
Toru Hyakutake
Keyword(s):  
Blood Cell ◽  
Red Blood Cells ◽  
Newtonian Fluid ◽  
Red Blood Cell ◽  
Blood Cells ◽  
Cell Model ◽  
Flow Behavior ◽  
Shear Thinning ◽  
Mutual Interference ◽  
Axial Concentration

We investigated how non-Newtonian viscosity behavior affects the flow characteristics of blood cells. Our findings offer insight about how shear thinning affects the dispersion of liposome-encapsulated hemoglobin and red blood cells in blood. The lattice Boltzmann method was used for fluid calculations, and the rheological properties of the non-Newtonian fluid were modeled with power-law relationships. The deformable three-dimensional red blood cell model was applied. First, we investigated the effects of shear thinning on the flow behavior of single blood cell. Simulation results indicate that shear thinning promotes the axial concentration of red blood cells. Next, varied the hematocrit to see how mutual interference between blood cells affects flow. At low hematocrit, shear thinning clearly promotes the axial concentration of red blood cells. As the hematocrit increases, in contrast, mutual interference has a greater effect, which counteracts shear thinning so the red blood cell distribution resembles the distribution within a Newtonian fluid.

Red blood cell (RBC) age at collection and storage influences RBC membrane-associated carbohydrates and lectin binding

Transfusion ◽  
2007 ◽  
Vol 47 (6) ◽  
pp. 966-968 ◽  
Author(s):  
Rosemary L. Sparrow ◽  
Margaret F. Veale ◽  
Geraldine Healey ◽  
Katherine A. Payne
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Lectin Binding ◽  
Rbc Membrane ◽  
And Storage

Red blood cell (RBC) membrane proteomics — Part I: Proteomics and RBC physiology

2010 ◽  
Vol 73 (3) ◽  
pp. 403-420 ◽  
Author(s):  
Erica M. Pasini ◽  
Hans U. Lutz ◽  
Matthias Mann ◽  
Alan W. Thomas
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Rbc Membrane ◽  
Membrane Proteomics

scholarly journals Exploring the gating mechanisms of aquaporin-3: new clues for the design of inhibitors?

2016 ◽  
Vol 12 (5) ◽  
pp. 1564-1573 ◽  
Author(s):  
A. de Almeida ◽  
A. P. Martins ◽  
A. F. Mósca ◽  
H. J. Wijma ◽  
C. Prista ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Model ◽  
Aquaporin 3 ◽  
Gating Mechanisms ◽  
First Time

The pH gating of human AQP3 and its effects on both water and glycerol permeabilities have been fully characterized for the first time using a human red blood cell model (hRBC).

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