scholarly journals Bio-Membrane Internalization Mechanisms of Arginine-Rich Cell-Penetrating Peptides in Various Species

Membranes ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 88
Author(s):  
Betty Revon Liu ◽  
Shiow-Her Chiou ◽  
Yue-Wern Huang ◽  
Han-Jung Lee
Keyword(s):  
Plasma Membranes ◽  
Cell Penetrating Peptides ◽  
Cellular Delivery ◽  
Drug Molecules ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Key Factor ◽  
Potential Applications ◽  
Primary Key ◽  
Potent Drug

Recently, membrane-active peptides or proteins that include antimicrobial peptides (AMPs), cytolytic proteins, and cell-penetrating peptides (CPPs) have attracted attention due to their potential applications in the biomedical field. Among them, CPPs have been regarded as a potent drug/molecules delivery system. Various cargoes, such as DNAs, RNAs, bioactive proteins/peptides, nanoparticles and drugs, can be carried by CPPs and delivered into cells in either covalent or noncovalent manners. Here, we focused on four arginine-rich CPPs and reviewed the mechanisms that these CPPs used for intracellular uptake across cellular plasma membranes. The varying transduction efficiencies of them alone or with cargoes were discussed, and the membrane permeability was also expounded for CPP/cargoes delivery in various species. Direct membrane translocation (penetration) and endocytosis are two principal mechanisms for arginine-rich CPPs mediated cargo delivery. Furthermore, the amino acid sequence is the primary key factor that determines the cellular internalization mechanism. Importantly, the non-cytotoxic nature and the wide applicability make CPPs a trending tool for cellular delivery.

scholarly journals Cell-Penetrating Peptides: A Comparative Study on Lipid Affinity and Cargo Delivery Properties

2010 ◽  
Vol 3 (4) ◽  
pp. 1045-1062 ◽  
Author(s):  
Paolo Ruzza ◽  
Barbara Biondi ◽  
Anna Marchiani ◽  
Nicola Antolini ◽  
Andrea Calderan
Keyword(s):  
Comparative Study ◽  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating

Cell‐penetrating peptides recruit type A scavenger receptors to the plasma membrane for cellular delivery of nucleic acids

2016 ◽  
Vol 31 (3) ◽  
pp. 975-988 ◽  
Author(s):  
Carmen Juks ◽  
Annely Lorents ◽  
Piret Arukuusk ◽  
Ülo Langel ◽  
Margus Pooga
Keyword(s):  
Plasma Membrane ◽  
Nucleic Acids ◽  
Type A ◽  
Cell Penetrating Peptides ◽  
Scavenger Receptors ◽  
Cellular Delivery ◽  
Cell Penetrating

scholarly journals The many futures for cell-penetrating peptides: how soon is now?

2007 ◽  
Vol 35 (4) ◽  
pp. 767-769 ◽  
Author(s):  
J. Howl ◽  
I.D. Nicholl ◽  
S. Jones
Keyword(s):  
Positive Impact ◽  
Biochemical Properties ◽  
Cell Penetrating Peptides ◽  
Protein Transduction Domains ◽  
Molecular Therapeutics ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Transactivator Of Transcription ◽  
The Many ◽  
Hiv 1

Studies of CPPs (cell-penetrating peptides), sequences that are also commonly designated as protein transduction domains, now extend to a second decade of exciting and far-reaching discoveries. CPPs are proven vehicles for the intracellular delivery of macromolecules that include oligonucleotides, peptides and proteins, low-molecular-mass drugs, nanoparticles and liposomes. The biochemical properties of different classes of CPP, including various sequences derived from the HIV-1 Tat (transactivator of transcription) [e.g. Tat-(48–60), GRKKRRQRRRPPQ], and the homeodomain of the Drosophila homeoprotein Antennapaedia (residues 43–58, commonly named penetratin, RQIKIWFQNRRMKWKK), also provide novel insights into the fundamental mechanisms of translocation across biological membranes. Thus the efficacy of CPP-mediated cargo delivery continues to provide valuable tools for biomedical research and, as witnessed in 2007, candidate and emerging therapeutics. Thus it is anticipated that the further refinement of CPP technologies will provide drug-delivery vectors, cellular imaging tools, nanoparticulate devices and molecular therapeutics that will have a positive impact on the healthcare arena. The intention of this article is to provide both a succinct overview of current developments and applications of CPP technologies, and to illustrate key developments that the concerted efforts of the many researchers contributing to the Biochemical Society's Focused Meeting in Telford predict for the future. The accompanying papers in this issue of Biochemical Society Transactions provide additional details and appropriate references. Hopefully, the important and eagerly anticipated biomedical and clinical developments within the CPP field will occur sooner rather than later.

scholarly journals Cargo delivery kinetics of cell-penetrating peptides

2001 ◽  
Vol 1515 (2) ◽  
pp. 101-109 ◽  
Author(s):  
Mattias Hällbrink ◽  
Anders Florén ◽  
Anna Elmquist ◽  
Margus Pooga ◽  
Tamas Bartfai ◽  
...  
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating ◽  
Kinetics Of

scholarly journals When cationic cell-penetrating peptides meet hydrocarbons to enhance in-cell cargo delivery

2015 ◽  
Vol 21 (5) ◽  
pp. 356-369 ◽  
Author(s):  
Margherita Di Pisa ◽  
Gérard Chassaing ◽  
Jean-Marie Swiecicki
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Cell Penetrating

scholarly journals Identification and characterization of novel enhanced cell penetrating peptides for anti-cancer cargo delivery

Oncotarget ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 5944-5957 ◽  
Author(s):  
Xiguang Zhang ◽  
Jean Yves Brossas ◽  
Christophe Parizot ◽  
Jean Marc Zini ◽  
Angelita Rebollo
Keyword(s):  
Cell Penetrating Peptides ◽  
Cargo Delivery ◽  
Anti Cancer ◽  
Cell Penetrating ◽  
Identification And Characterization

scholarly journals The Utilization of Cell-Penetrating Peptides in the Intracellular Delivery of Viral Nanoparticles

Materials ◽  
2019 ◽  
Vol 12 (17) ◽  
pp. 2671 ◽  
Author(s):  
Jana Váňová ◽  
Alžběta Hejtmánková ◽  
Marie Hubálek Kalbáčová ◽  
Hana Španielová
Keyword(s):  
Genetic Material ◽  
Cell Types ◽  
Cell Penetrating Peptides ◽  
Target Cells ◽  
Current State ◽  
Cargo Delivery ◽  
Viral Particles ◽  
Cell Penetrating ◽  
Viral Nanoparticles ◽  
Immunogenic Properties

Viral particles (VPs) have evolved so as to efficiently enter target cells and to deliver their genetic material. The current state of knowledge allows us to use VPs in the field of biomedicine as nanoparticles that are safe, easy to manipulate, inherently biocompatible, biodegradable, and capable of transporting various cargoes into specific cells. Despite the fact that these virus-based nanoparticles constitute the most common vectors used in clinical practice, the need remains for further improvement in this area. The aim of this review is to discuss the potential for enhancing the efficiency and versatility of VPs via their functionalization with cell-penetrating peptides (CPPs), short peptides that are able to translocate across cellular membranes and to transport various substances with them. The review provides and describes various examples of and means of exploitation of CPPs in order to enhance the delivery of VPs into permissive cells and/or to allow them to enter a broad range of cell types. Moreover, it is possible that CPPs are capable of changing the immunogenic properties of VPs, which could lead to an improvement in their clinical application. The review also discusses strategies aimed at the modification of VPs by CPPs so as to create a useful cargo delivery tool.

scholarly journals Cargo-dependent cytotoxicity and delivery efficacy of cell-penetrating peptides: a comparative study

2007 ◽  
Vol 407 (2) ◽  
pp. 285-292 ◽  
Author(s):  
Samir El-Andaloussi ◽  
Peter Järver ◽  
Henrik J. Johansson ◽  
Ülo Langel
Keyword(s):  
Membrane Integrity ◽  
Cell Penetrating Peptides ◽  
Bioactive Molecules ◽  
Cargo Delivery ◽  
Wide Range ◽  
Cell Penetrating ◽  
Coupling Strategy ◽  
Transactivator Of Transcription

The use of CPPs (cell-penetrating peptides) as delivery vectors for bioactive molecules has been an emerging field since 1994 when the first CPP, penetratin, was discovered. Since then, several CPPs, including the widely used Tat (transactivator of transcription) peptide, have been developed and utilized to translocate a wide range of compounds across the plasma membrane of cells both in vivo and in vitro. Although the field has emerged as a possible future candidate for drug delivery, little attention has been given to the potential toxic side effects that these peptides might exhibit in cargo delivery. Also, no comprehensive study has been performed to evaluate the relative efficacy of single CPPs to convey different cargos. Therefore we selected three of the major CPPs, penetratin, Tat and transportan 10, and evaluated their ability to deliver commonly used cargos, including fluoresceinyl moiety, double-stranded DNA and proteins (i.e. avidin and streptavidin), and studied their effect on membrane integrity and cell viability. Our results demonstrate the unfeasibility to use the translocation efficacy of fluorescein moiety as a gauge for CPP efficiency, since the delivery properties are dependent on the cargo used. Furthermore, and no less importantly, the toxicity of CPPs depends heavily on peptide concentration, cargo molecule and coupling strategy.

scholarly journals Cell-Penetrating Peptides Derived from Animal Venoms and Toxins

Toxins ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 147
Author(s):  
Gandhi Rádis-Baptista
Keyword(s):  
Plasma Membranes ◽  
Cytoplasmic Membrane ◽  
De Novo ◽  
Cell Types ◽  
Cell Penetrating Peptides ◽  
Cumulative Number ◽  
Cell Cytoplasm ◽  
Cell Penetrating ◽  
Animal Venoms ◽  
Pharmaceutical Biotechnology

Cell-penetrating peptides (CPPs) comprise a class of short polypeptides that possess the ability to selectively interact with the cytoplasmic membrane of certain cell types, translocate across plasma membranes and accumulate in the cell cytoplasm, organelles (e.g., the nucleus and mitochondria) and other subcellular compartments. CPPs are either of natural origin or de novo designed and synthesized from segments and patches of larger proteins or designed by algorithms. With such intrinsic properties, along with membrane permeation, translocation and cellular uptake properties, CPPs can intracellularly convey diverse substances and nanomaterials, such as hydrophilic organic compounds and drugs, macromolecules (nucleic acids and proteins), nanoparticles (nanocrystals and polyplexes), metals and radionuclides, which can be covalently attached via CPP N- and C-terminals or through preparation of CPP complexes. A cumulative number of studies on animal toxins, primarily isolated from the venom of arthropods and snakes, have revealed the cell-penetrating activities of venom peptides and toxins, which can be harnessed for application in biomedicine and pharmaceutical biotechnology. In this review, I aimed to collate examples of peptides from animal venoms and toxic secretions that possess the ability to penetrate diverse types of cells. These venom CPPs have been chemically or structurally modified to enhance cell selectivity, bioavailability and a range of target applications. Herein, examples are listed and discussed, including cysteine-stabilized and linear, α-helical peptides, with cationic and amphipathic character, from the venom of insects (e.g., melittin, anoplin, mastoparans), arachnids (latarcin, lycosin, chlorotoxin, maurocalcine/imperatoxin homologs and wasabi receptor toxin), fish (pardaxins), amphibian (bombesin) and snakes (crotamine and cathelicidins).

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