scholarly journals Interactions Screenings Unearth Potential New Divisome Components in the Chlamydia-Related Bacterium, Waddlia chondrophila

2019 ◽  
Vol 7 (12) ◽  
pp. 617
Author(s):  
Firuza Bayramova ◽  
Nicolas Jacquier ◽  
Gilbert Greub
Keyword(s):  
Iron Homeostasis ◽  
Expression Profiles ◽  
Model Organism ◽  
E Coli ◽  
Obligate Intracellular ◽  
Gene And Protein Expression ◽  
Related Bacterium ◽  
Obligate Intracellular Bacteria ◽  
Protein Expression Profiles ◽  
Two Hybrid

Chlamydiales order members are obligate intracellular bacteria, dividing by binary fission. However, Chlamydiales lack the otherwise conserved homologue of the bacterial division organizer FtsZ and certain division protein homologues. FtsZ might be functionally replaced in Chlamydiales by the actin homologue MreB. RodZ, the membrane anchor of MreB, localizes early at the division septum. In order to better characterize the organization of the chlamydial divisome, we performed co-immunoprecipitations and yeast-two hybrid assays to study the interactome of RodZ, using Waddlia chondrophila, a potentially pathogenic Chlamydia-related bacterium, as a model organism. Three potential interactors were further investigated: SecA, FtsH, and SufD. The gene and protein expression profiles of these three genes were measured and are comparable with recently described division proteins. Moreover, SecA, FtsH, and SufD all showed a peripheral localization, consistent with putative inner membrane localization and interaction with RodZ. Notably, heterologous overexpression of the abovementioned proteins could not complement E. coli mutants, indicating that these proteins might play different functions in these two bacteria or that important regulators are not conserved. Altogether, this study brings new insights to the composition of the chlamydial divisome and points to links between protein secretion, degradation, iron homeostasis, and chlamydial division.

Gene and protein expression profiles of JAK-STAT signalling pathway in the developing brain of the Ts1Cje down syndrome mouse model

2019 ◽  
Vol 129 (9) ◽  
pp. 871-881 ◽  
Author(s):  
Han-Chung Lee ◽  
Hadri Hadi Md Yusof ◽  
Melody Pui-Yee Leong ◽  
Shahidee Zainal Abidin ◽  
Eryse Amira Seth ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Mouse Model ◽  
Protein Expression ◽  
Expression Profiles ◽  
Developing Brain ◽  
Signalling Pathway ◽  
Gene And Protein Expression ◽  
Protein Expression Profiles

Gene and Protein Expression Profiles ofShewanella oneidensisduring Anaerobic Growth with Different Electron Acceptors

2002 ◽  
Vol 6 (1) ◽  
pp. 39-60 ◽  
Author(s):  
Alex S. Beliaev ◽  
Dorothea K. Thompson ◽  
Tripti Khare ◽  
Hanjo Lim ◽  
Craig C. Brandt ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Profiles ◽  
Electron Acceptors ◽  
Anaerobic Growth ◽  
Gene And Protein Expression ◽  
Protein Expression Profiles

scholarly journals Cellular Functions and Gene and Protein Expression Profiles in Endothelial Cells Derived from Moyamoya Disease-Specific iPS Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163561 ◽  
Author(s):  
Shuji Hamauchi ◽  
Hideo Shichinohe ◽  
Haruto Uchino ◽  
Shigeru Yamaguchi ◽  
Naoki Nakayama ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Protein Expression ◽  
Moyamoya Disease ◽  
Expression Profiles ◽  
Ips Cells ◽  
Cellular Functions ◽  
Gene And Protein Expression ◽  
Protein Expression Profiles ◽  
Disease Specific

scholarly journals Global Molecular and Morphological Effects of 24-Hour Chromium(VI) Exposure on Shewanella oneidensis MR-1

2006 ◽  
Vol 72 (9) ◽  
pp. 6331-6344 ◽  
Author(s):  
Karuna Chourey ◽  
Melissa R. Thompson ◽  
Jennifer Morrell-Falvey ◽  
Nathan C. VerBerkmoes ◽  
Steven D. Brown ◽  
...  
Keyword(s):  
Untreated Control ◽  
Expression Profiles ◽  
Shewanella Oneidensis ◽  
Transcriptome Profiling ◽  
Molecular Response ◽  
Chromium Vi ◽  
Stress Protection ◽  
Gene And Protein Expression ◽  
Genes Encoding ◽  
Protein Expression Profiles

ABSTRACT The biological impact of 24-h (“chronic”) chromium(VI) [Cr(VI) or chromate] exposure on Shewanella oneidensis MR-1 was assessed by analyzing cellular morphology as well as genome-wide differential gene and protein expression profiles. Cells challenged aerobically with an initial chromate concentration of 0.3 mM in complex growth medium were compared to untreated control cells grown in the absence of chromate. At the 24-h time point at which cells were harvested for transcriptome and proteome analyses, no residual Cr(VI) was detected in the culture supernatant, thus suggesting the complete uptake and/or reduction of this metal by cells. In contrast to the untreated control cells, Cr(VI)-exposed cells formed apparently aseptate, nonmotile filaments that tended to aggregate. Transcriptome profiling and mass spectrometry-based proteomic characterization revealed that the principal molecular response to 24-h Cr(VI) exposure was the induction of prophage-related genes and their encoded products as well as a number of functionally undefined hypothetical genes that were located within the integrated phage regions of the MR-1 genome. In addition, genes with annotated functions in DNA metabolism, cell division, biosynthesis and degradation of the murein (peptidoglycan) sacculus, membrane response, and general environmental stress protection were upregulated, while genes encoding chemotaxis, motility, and transport/binding proteins were largely repressed under conditions of 24-h chromate treatment.

scholarly journals Genome rearrangements induce biofilm formation inEscherichia coliC – an old model organism with a new application in biofilm research

2019 ◽  
Author(s):  
Jarosław E. Król ◽  
Donald C. Hall ◽  
Sergey Balashov ◽  
Steven Pastor ◽  
Justin Siebert ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Insertion Sequence ◽  
Genomic Sequence ◽  
Expression Profiles ◽  
Model Organism ◽  
The Other ◽  
Start Codon ◽  
Bacterial Survival ◽  
E Coli ◽  
Full Genomic Sequence

AbstractEscherichia coliC forms more robust biofilms than the other laboratory strains. Biofilm formation and cell aggregation under a high shear force depends on temperature and salt concentrations. It is the last of fiveE. colistrains (C, K12, B, W, Crooks) designated as safe for laboratory purposes whose genome has not been sequenced. Here we present the complete genomic sequence of this strain in which we utilized both long-read PacBio-based sequencing and high resolution optical mapping to confirm a large inversion in comparison to the other laboratory strains. Notably, DNA sequence comparison revealed the absence of several genes thought to be involved in biofilm formation, including antigen 43,waaSBOJYZULfor LPS synthesis, andcpsBfor curli synthesis. The first main difference we identified that likely affects biofilm formation is the presence of an IS3-like insertion sequence in front of the carbon storage regulatorcsrAgene. This insertion is located 86 bp upstream of thecsrAstart codon inside the −35 region of P4 promoter and blocks the transcription from the sigma32and sigma70promoters P1-P3 located further upstream. The second is the presence of an IS5/IS1182 in front of thecsgDgene, which may drive its overexpression in biofilm. And finally,E. coliC encodes an additional sigma70subunit overexpressed in biofilm and driven by the same IS3-like insertion sequence. Promoter analyses using GFP gene fusions and total expression profiles using RNA-seq analyses comparing planktonic and biofilm envirovars provided insights into understanding this regulatory pathway inE. coli.IMPORTANCEBiofilms are crucial for bacterial survival, adaptation, and dissemination in natural, industrial, and medical environments. Most laboratory strains ofE. coligrown for decadesin vitrohave evolved and lost their ability to form biofilm, while environmental isolates that can cause infections and diseases are not safe to work with. Here, we show that the historic laboratory strain ofE. coliC produces a robust biofilm and can be used as a model organism for multicellular bacterial research. Furthermore, we ascertained the full genomic sequence as well as gene expression profiles of both the biofilm and planktonic envirovars of this classic strain, which provide for a base level of characterization and make it useful for many biofilm-based applications.

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