scholarly journals Optimised Production and Extraction of Astaxanthin from the Yeast Xanthophyllomyces dendrorhous

2020 ◽  
Vol 8 (3) ◽  
pp. 430 ◽  
Author(s):  
Zuharlida Tuan Harith ◽  
Micael de Andrade Lima ◽  
Dimitris Charalampopoulos ◽  
Afroditi Chatzifragkou
Keyword(s):  
Agitation Speed ◽  
Xanthophyllomyces Dendrorhous ◽  
Alternative Pathways ◽  
Extraction Solvent ◽  
Commercial Enzymes ◽  
Bioreactor Operation ◽  
Astaxanthin Production ◽  
Ph Conditions ◽  
Central Composite ◽  
Astaxanthin Extraction

Currently, astaxanthin demand is fulfilled by chemical synthesis using petroleum-based feedstocks. As such, alternative pathways of natural astaxanthin production attracts much research interest. This study aimed at optimising bioreactor operation parameters for astaxanthin production and evaluating strategies for its subsequent extraction. The effect of pH and agitation was evident, as a significant reduction in both biomass and astaxanthin production was observed when the culture pH was not controlled and a low agitation speed was applied. At controlled pH conditions and a high agitation speed, a significant increase in biomass (16.4 g/L) and astaxanthin production (3.6 mg/L) was obtained. Enzymatic yeast cell lysis using two commercial enzymes (Accellerase 1500 and Glucanex) was optimised using the central composite design of experiment (DoE). Accellerase 1500 led to mild cell disruption and only 9% (w/w) astaxanthin extraction. However, Glucanex treatment resulted in complete astaxanthin extractability, compared to standard extraction method (DMSO/acetone). When supercritical CO2 was employed as an extraction solvent in Accellerase-pre-treated Xanthophyllomyces dendrorhous cells, astaxanthin extraction increased 2.5-fold. Overall, the study showed that extraction conditions can be tailored towards targeted pigments present in complex mixtures, such as in microbial cells.

Citrus Residues Isolates Improve Astaxanthin Production by Xanthophyllomyces dendrorhous

2010 ◽  
Vol 65 (9-10) ◽  
pp. 594-598 ◽  
Author(s):  
Wei Wu ◽  
Mingbo Lu ◽  
Longjiang Yu
Keyword(s):  
Carbon Source ◽  
Yeast Extract ◽  
Minimal Medium ◽  
Wild Strain ◽  
Xanthophyllomyces Dendrorhous ◽  
Citrus Juice ◽  
Astaxanthin Production ◽  
Mutant Strains

The wild strain and two astaxanthin-overproducing mutant strains, W618 and GNG274, of Xanthophyllomyces dendrorhous were analyzed in order to assess their ability to grow and synthesize astaxanthin in a minimal medium containing (per liter): 2 g KH2PO4, 0.5 g MgSO4, 2 g KNO3, and 1 g yeast extract, and supplemented with citrus residues isolates as a carbon source (citrus medium). The selected strain W618 was evaluated under various contents of citrus juice. At the content of 20% (v/v), the highest astaxanthin production reached 22.63 mg L-1, which was two-fold more than that observed in yeast malt medium. Addition of 8% (v/v) n-hexadecane to the citrus medium was found to be optimal, increasing the astaxanthin yield by 21.7%.

Astaxanthin production by Xanthophyllomyces dendrorhous growing on a low cost substrate

2019 ◽  
Vol 94 (4) ◽  
pp. 1229-1234 ◽  
Author(s):  
Teresa Gervasi ◽  
Antonello Santini ◽  
Patricia Daliu ◽  
Abdelfattah Z. M. Salem ◽  
Claudio Gervasi ◽  
...  
Keyword(s):  
Low Cost ◽  
Xanthophyllomyces Dendrorhous ◽  
Astaxanthin Production ◽  
Low Cost Substrate

Bioreactor operation parameters as tools for metabolic regulations in fermentation processes: influence of pH conditions

2003 ◽  
Vol 58 (3-6) ◽  
pp. 759-766 ◽  
Author(s):  
Pinar Çalik ◽  
Esra Bilir ◽  
Güzide Çalik ◽  
Tunçer H Özdamar
Keyword(s):  
Fermentation Processes ◽  
Bioreactor Operation ◽  
Ph Conditions ◽  
Operation Parameters ◽  
Influence Of Ph

scholarly journals The Optimised Statistical Model for Enzymatic Hydrolysis of Tapioca by Glucoamylase Immobilised on Mesostructured Cellular Foam Silica

2019 ◽  
Vol 14 (2) ◽  
pp. 380
Author(s):  
Joni Agustian ◽  
Lilis Hermida
Keyword(s):  
Enzymatic Hydrolysis ◽  
Large Scale ◽  
Specific Activity ◽  
Agitation Speed ◽  
Quadratic Model ◽  
Lack Of Fit ◽  
Km Value ◽  
Hydrolysis Of ◽  
Central Composite ◽  
Better Than

Enzymatic hydrolysis of starches using free glucoamylase to reducing sugars have difficulties in recovering and recycling of the enzyme, hence immobilisation on inert supports were widely studied. However, effectiveness of the immobilised glucoamylase were merely observed only on soluble starches. It was considered a valuable thing to know performance of glucoamylase on Mesostructured Cellular Foam (MCF) silica in hydrolysing of tapioca. An optimised study on enzymatic hydrolysis of tapioca using glucoamylase on MCF silica (9.2T-3D) and its kinetics were described including justification of the predicted model as it was required to develop in large scale operations. Central Composite Design was used to model the process by studying effects of three factors on DE values after enzyme immobilisation.  Immobilisation of glucoamylase on this support gave up to 82% efficiency with the specific activity of 1,856.78 U.g-1. Its used to hydrolysis of tapioca resulted DE values of 1.740-76.303% (w/w) where the highest DE was obtained at pH of 4.1, temperature of 70 ℃ and agitation speed of 140 rpm. The optimisation produced a polynomial quadratic model having insignificant lack-of-fit and low standard deviation, so that it was applicable and reliable in simulating the DE with only 0.80% of data were not described. Temperature affected the process highly, but the buffer pH, agitation speed and factorial interactions were considered not important. KM value for immobilised enzyme was better than the free glucoamylase, however, its reaction rate was slower than the free glucoamylase catalysis. Copyright © 2019 BCREC Group. All rights reserved 

A Kinetic Model for Astaxanthin Fermentation by Xanthophyllomyces Dendrorhous Zjut46

2011 ◽  
Vol 343-344 ◽  
pp. 397-402 ◽  
Author(s):  
Zhong Ce Hu ◽  
Guo Feng Jiang ◽  
Yu Guo Zheng
Keyword(s):  
Batch Fermentation ◽  
Microbial Growth ◽  
Calculated Data ◽  
Glucose Consumption ◽  
Logistic Equation ◽  
Xanthophyllomyces Dendrorhous ◽  
Carotenoid Pigment ◽  
Fermentation System ◽  
Astaxanthin Production ◽  
Kinetics Of

Astaxanthin, a main carotenoid pigment, has a strong antioxidant activity. A kinetics of astaxanthin fermentation by Xanthophyllomyces dendrorhous was studied in a batch fermentation system. The unstructured models were proposed using the Logistic equation for microbial growth, the Luedeking-Piret equation for astaxanthin production and Luedeking-Piret-like equation for glucose consumption. The production of astaxanthin was partly growth-associated. Compared with the experimental data and calculated data, most of errors were lower than 10 %, it showed that the proposed unstructured models were adequate to describe the fermentation bioprocess of astaxanthin by X. dendrorhous.

scholarly journals Adsorption of crystal violet from aqueous solution onto sugarcane bagasse: central composite design for optimization of process variables

2012 ◽  
Vol 2 (1) ◽  
pp. 55-65 ◽  
Author(s):  
Sagnik Chakraborty ◽  
Shamik Chowdhury ◽  
Papita Das Saha
Keyword(s):  
Aqueous Solution ◽  
Central Composite Design ◽  
Sugarcane Bagasse ◽  
Crystal Violet ◽  
Agitation Speed ◽  
Initial Solution ◽  
Optimum Conditions ◽  
Solution Ph ◽  
Central Composite ◽  
Adsorbent Dose

The adsorption of crystal violet (CV) from aqueous solution by sugarcane bagasse (SCB), an agro-industrial residue, was investigated in a batch experimental setup. A two level four factor (24) full factorial central composite design (CCD) with the help of Design Expert Version 7.1.6 (Stat-Ease, USA) was used for adsorption process optimization and evaluation of interaction effects of different operating parameters: agitation speed (80–180 rpm), initial solution pH (4.0–8.0), initial dye concentration (100–200 mg L–1), and adsorbent dose (2–5 g L–1). A multiple coefficient of determination (R2) value of 0.98, model F value of 266.36 and its low P-value (<0.0001) along with lower value of coefficient of variation (2.70%) indicated the fitness of the response surface quadratic model developed during the present study. Numerical optimization applying desirability function was used to identify the optimum conditions for maximum removal of CV. The optimum conditions were found to be agitation speed = 165 rpm, initial solution pH = 8.0, initial dye concentration = 200 mg L–1 and adsorbent dose = 2.0 g L–1. A confirmatory experiment was performed to evaluate the accuracy of the optimization procedure and maximum CV removal of 93.21% was achieved under the optimized conditions.

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