scholarly journals Carbon Monoxide Induced Metabolic Shift in the Carboxydotrophic Parageobacillus thermoglucosidasius DSM 6285

2021 ◽  
Vol 9 (5) ◽  
pp. 1090
Author(s):  
Habibu Aliyu ◽  
Ronnie Kastner ◽  
Pieter de Maayer ◽  
Anke Neumann
Keyword(s):  
Carbon Monoxide ◽  
Alternative Energy ◽  
Redox Balance ◽  
Biotechnological Potential ◽  
Alternative Pathways ◽  
Biological Water ◽  
Wgs Reaction ◽  
Water Gas ◽  
Further Development ◽  
Parageobacillus Thermoglucosidasius

Parageobacillus thermoglucosidasius is known to catalyse the biological water gas shift (WGS) reaction, a pathway that serves as a source of alternative energy and carbon to a wide variety of bacteria. Despite increasing interest in this bacterium due to its ability to produce biological hydrogen through carbon monoxide (CO) oxidation, there are no data on the effect of toxic CO gas on its physiology. Due to its general requirement of O2, the organism is often grown aerobically to generate biomass. Here, we show that carbon monoxide (CO) induces metabolic changes linked to distortion of redox balance, evidenced by increased accumulation of organic acids such as acetate and lactate. This suggests that P. thermoglucosidasius survives by expressing several alternative pathways, including conversion of pyruvate to lactate, which balances reducing equivalents (oxidation of NADH to NAD+), and acetyl-CoA to acetate, which directly generates energy, while CO is binding terminal oxidases. The data also revealed clearly that P. thermoglucosidasius gained energy and grew during the WGS reaction. Combined, the data provide critical information essential for further development of the biotechnological potential of P. thermoglucosidasius.

scholarly journals Time-course Transcriptome of Parageobacillus thermoglucosidasius DSM 6285 Grown in the Presence of Carbon Monoxide and Air

2020 ◽  
Vol 21 (11) ◽  
pp. 3870
Author(s):  
Habibu Aliyu ◽  
Teresa Mohr ◽  
Don Cowan ◽  
Pieter de Maayer ◽  
Anke Neumann
Keyword(s):  
Carbon Monoxide ◽  
Time Course ◽  
Anaerobic Growth ◽  
Post Inoculation ◽  
Wgs Reaction ◽  
Gas Atmosphere ◽  
Flagellar Assembly ◽  
Growth Profiles ◽  
Water Gas ◽  
Parageobacillus Thermoglucosidasius

Parageobacillus thermoglucosidasius is a metabolically versatile, facultatively anaerobic thermophile belonging to the family Bacillaceae. Previous studies have shown that this bacterium harbours co-localised genes coding for a carbon monoxide (CO) dehydrogenase (CODH) and Ni-Fe hydrogenase (Phc) complex and oxidises CO and produces hydrogen (H2) gas via the water-gas shift (WGS) reaction. To elucidate the genetic events culminating in the WGS reaction, P. thermoglucosidasius DSM 6285 was cultivated under an initial gas atmosphere of 50% CO and 50% air and total RNA was extracted at ~8 (aerobic phase), 20 (anaerobic phase), 27 and 44 (early and late hydrogenogenic phases) hours post inoculation. The rRNA-depleted fraction was sequenced using Illumina NextSeq, v2.5, 1x75bp chemistry. Differential expression revealed that at 8 vs.. 20, 20 vs.. 27 and 27 vs.. 44 h post inoculation, 2190, 2118 and 231 transcripts were differentially (FDR < 0.05) expressed. Cluster analysis revealed 26 distinct gene expression trajectories across the four time points. Of these, two similar clusters, showing overexpression at 20 relative to 8 h and depletion at 27 and 44 h, harboured the CODH and Phc transcripts, suggesting possible regulation by O2. The transition between aerobic respiration and anaerobic growth was marked by initial metabolic deterioration, as reflected by up-regulation of transcripts linked to sporulation and down-regulation of transcripts linked to flagellar assembly and metabolism. However, the transcriptome and growth profiles revealed the reversal of this trend during the hydrogenogenic phase.

scholarly journals Not All That Glitters Is Gold: The Paradox of CO-dependent Hydrogenogenesis in Parageobacillus thermoglucosidasius

2021 ◽  
Vol 12 ◽  
Author(s):  
Habibu Aliyu ◽  
Pieter de Maayer ◽  
Anke Neumann
Keyword(s):  
Carbon Monoxide ◽  
Iron Acquisition ◽  
Thermophilic Bacterium ◽  
Genomic Region ◽  
Water Gas Shift ◽  
Air Atmosphere ◽  
Shift Reaction ◽  
Genomic Regions ◽  
Water Gas ◽  
Parageobacillus Thermoglucosidasius

The thermophilic bacterium Parageobacillus thermoglucosidasius has recently gained interest due to its ability to catalyze the water gas shift reaction, where the oxidation of carbon monoxide (CO) is linked to the evolution of hydrogen (H2) gas. This phenotype is largely predictable based on the presence of a genomic region coding for a carbon monoxide dehydrogenase (CODH—Coo) and hydrogen evolving hydrogenase (Phc). In this work, seven previously uncharacterized strains were cultivated under 50% CO and 50% air atmosphere. Despite the presence of the coo—phc genes in all seven strains, only one strain, Kp1013, oxidizes CO and yields H2. The genomes of the H2 producing strains contain unique genomic regions that code for proteins involved in nickel transport and the detoxification of catechol, a by-product of a siderophore-mediated iron acquisition system. Combined, the presence of these genomic regions could potentially drive biological water gas shift (WGS) reaction in P. thermoglucosidasius.

Development of a Medium-Size Steam Reformer for CHP Applications Based on PEM Fuel Cells

2010 ◽  
Author(s):  
Giovanni Pisani ◽  
Alberto Zerbinato ◽  
Carlo Tregambe ◽  
Ernesto Benini
Keyword(s):  
Carbon Monoxide ◽  
Hydrogen Production ◽  
Pem Fuel Cells ◽  
Water Gas Shift ◽  
Medium Size ◽  
Steam Reformer ◽  
Fuel Processor ◽  
Conversion Performance ◽  
Conversion Efficiencies ◽  
Water Gas

This paper describes technological of a fuel processor for hydrogen production able to convert 10 cubic meters of methane per hour. This device has been developed to feed hydrogen CHP suitable for the most common residential applications. The measured conversion efficiencies are extremely high: after the steam reformer the results are 76%H2; 18%CO2; 0,5%CH4; 5%CO; but the carbon monoxide is totally reduced throughout the water gas shift and the partial oxidation which contemporarily increase the hydrogen to over 77%. According to these results, this fuel processor is one of the first middle sized reformer to achieve, at comparable costs per cubic meter, conversion performance that were normally obtained only by industrial reforming plants.

Facet-dependent activity of shape-controlled TiO2 supported Au nanoparticles for water-gas shift reaction†

2022 ◽  
Author(s):  
Yuanting Tang ◽  
Yongjie Chen ◽  
Xiao Liu ◽  
Chengxiong Wang ◽  
Yunkun Zhao ◽  
...  
Keyword(s):  
Au Nanoparticles ◽  
Water Gas Shift ◽  
Water Gas Shift Reaction ◽  
Oxide Interface ◽  
Wgs Reaction ◽  
Dependent Activity ◽  
Shape Controlled ◽  
Shift Reaction ◽  
Water Gas

The bifunctional role of noble metal/oxide interface in the activation of reactants is of vital importance in heterogeneous catalysis of water-gas shift (WGS) reaction. Herein, three types of shape-controlled TiO2...

scholarly journals Cr-Free, Cu Promoted Fe Oxide-Based Catalysts for High-Temperature Water-Gas Shift (HT-WGS) Reaction

Catalysts ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 305
Author(s):  
Sagar Sourav ◽  
Israel E. Wachs
Keyword(s):  
Active Sites ◽  
Catalyst Surface ◽  
Analysis Data ◽  
Fe Oxide ◽  
Redox Sites ◽  
Wgs Reaction ◽  
Catalytic Active Sites ◽  
Redox Ability ◽  
Water Gas ◽  
Temperature Water

Ca, Ni, Co, and Ge promoters were examined as potential candidates to substitute for the current toxic Cr in Cu-promoted Fe oxide-based catalysts for the HT-WGS reaction. The Ca and Ni promoters were found to improve catalyst performance relative to promotion with Cr. The HS-LEIS surface analysis data demonstrate that Ca and Ge tend to segregate on the surface, while Ni, Co, and Cr form solid solutions in the Fe3O4 bulk lattice. The corresponding number of catalytic active sites, redox, and WGS activity values of the catalysts were determined with CO-TPR, CO+H2O-TPSR, and SS-WGS studies, respectively. The poorer HT-WGS performances of the Ge and Co promoters are related to the presence of surface Ge and Co that inhibits catalyst redox ability, with the Co also not stabilizing the surface area of the Fe3O4 support. The Ni promoter uniformly disperses the Cu nanoparticles on the catalyst surface and increases the number of FeOx-Cu interfacial redox sites. The Ca promoter on the catalyst surface, however, enhances the activity of the FeOx-Cu interfacial redox sites. The CO+H2O TPSR results reveal that the redox ability of the active sites follows the SS-WGS performance of the catalysts and show the following trend: 3Cu8CaFe > 3Cu8NiFe ≥ 3Cu8CrFe > 3Cu8CoFe >> 3Cu8GeFe. Furthermore, all the catalysts followed a redox-type reaction mechanism for the HT-WGS reaction.

Outdoor sunlight-driven scalable water-gas shift reaction through novel photothermal device-supported CuOx/ZnO/Al2O3 nanosheets with a hydrogen generation rate of 192 mmol g−1 h−1

2020 ◽  
Vol 8 (37) ◽  
pp. 19467-19472
Author(s):  
Chengcheng Shi ◽  
Dachao Yuan ◽  
Luping Ma ◽  
Yaguang Li ◽  
Yangfan Lu ◽  
...  
Keyword(s):  
Production Rate ◽  
Light Absorption ◽  
Hydrogen Generation ◽  
Water Gas Shift ◽  
Generation Rate ◽  
Water Gas Shift Reaction ◽  
Wgs Reaction ◽  
Shift Reaction ◽  
Water Gas

A new photothermal device is constructed by the Cr based selective light absorption film which can heat the 2D CuZnAl to 297 °C under 1 standard sun irradiation, leading to a 192 mmol g−1 h−1 of H2 production rate from 1 sun irradiated WGS reaction.

Activity of Au, Ni, and Au-Ni catalysts in the water-gas shift reaction and carbon monoxide oxidation

2014 ◽  
Vol 55 (3) ◽  
pp. 311-318 ◽  
Author(s):  
S. A. Nikolaev ◽  
E. V. Golubina ◽  
L. M. Kustov ◽  
A. L. Tarasov ◽  
O. P. Tkachenko
Keyword(s):  
Carbon Monoxide ◽  
Carbon Monoxide Oxidation ◽  
Water Gas Shift ◽  
Water Gas Shift Reaction ◽  
Ni Catalysts ◽  
Shift Reaction ◽  
Water Gas
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