scholarly journals Reporter-Phage-Based Detection and Antibiotic Susceptibility Testing of Yersinia pestis for a Rapid Plague Outbreak Response

2021 ◽  
Vol 9 (6) ◽  
pp. 1278
Author(s):  
Sarit Moses ◽  
Moshe Aftalion ◽  
Emanuelle Mamroud ◽  
Shahar Rotem ◽  
Ida Steinberger-Levy
Keyword(s):  
Yersinia Pestis ◽  
Antibiotic Susceptibility ◽  
Direct Method ◽  
Rapid Identification ◽  
Outbreak Response ◽  
Bacterial Isolation ◽  
Correct Treatment ◽  
Antibiotic Susceptibility Test ◽  
Tier 1 ◽  
Human Blood Cultures

Pneumonic plague is a lethal infectious disease caused by Yersinia pestis, a Tier-1 biothreat agent. Antibiotic treatment can save infected patients; however, therapy should begin within 24 h of symptom onset. As some Y. pestis strains showed an antibiotic resistance phenotype, an antibiotic susceptibility test (AST) must be performed. Performing the Clinical and Laboratory Standards Institute (CLSI)-recommended standard process, which includes bacterial isolation, enumeration and microdilution testing, lasts several days. Thus, rapid AST must be developed. As previously published, the Y. pestis-specific reporter phage ϕA1122::luxAB can serve for rapid identification and AST (ID-AST). Herein, we demonstrate the ability to use ϕA1122::luxAB to determine minimal inhibitory concentration (MIC) values and antibiotic susceptibility categories for various Y. pestis therapeutic antibiotics. We confirmed the assay by testing several nonvirulent Y. pestis isolates with reduced susceptibility to doxycycline or ciprofloxacin. Moreover, the assay can be performed directly on positive human blood cultures. Furthermore, as Y. pestis may naturally or deliberately be spread in the environment, we demonstrate the compatibility of this direct method for this scenario. This direct phage-based ID-AST shortens the time needed for standard AST to less than a day, enabling rapid and correct treatment, which may also prevent the spread of the disease.

scholarly journals Outbreak and management of Salmonella Enteritidis infection in 2-week-old Lohmann brown pullets

2020 ◽  
Vol 18 (3) ◽  
pp. 175-180
Author(s):  
O.O. Oladele ◽  
N.O. Ameji ◽  
G.Y. Gurumyen ◽  
O.D. Omoniwa ◽  
E Joseph
Keyword(s):  
Antibiotic Susceptibility ◽  
Salmonella Enteritidis ◽  
Inflammatory Cells ◽  
Health Impact ◽  
Susceptibility Test ◽  
Macconkey Agar ◽  
Bacterial Isolation ◽  
Antibiotic Susceptibility Test ◽  
Microbial Analysis ◽  
Renal Tubular

Avian salmonellosis has huge economic and public health impact. In this manuscript, a case of S. Enteritidis and its management within Jos Metropolis was reported. Fiftythree carcasses of 2-weeks old pullets were presented at the poultry and fish clinic of the Veterinary Teaching Hospital, University of Jos, Nigeria for investigation. There was persistent mortality despite 5 days medication with 20% Enrofloxacin (Floxinor®, Shijiazhuang Guanghua Pharmaceutical co. Ltd, Hebei, China). Cumulative mortality within 14 days was 203 birds in a flock of 4,000. Necropsy was done and harvested were subjected to microbial analysis for bacterial isolation, identification and antibiotic susceptibility test while portions of these organs were preserved in 10% formalin for histopathology. Necropsy findings were empty crops, hepatitis with petechial hemorrhages, nephritis, congested and consolidated lungs, peritonitis, congested spleens and mild enteritis. Histologically, there were vacuolation and necrosis of renal tubular epithelia cells and interstitial infiltration with heterophils. Severe disorganization of hepatic cords, infiltration with inflammatory cells and mild necrosis of hepatocytes were observed, while there was severe congestion and diffuse hemorrhages in the lungs. Cellular infiltration within the lamina propria of small intestine with stunting and blunting of the villi were observed. Organism isolated on MacConkey agar was identified as Salmonella Enteritidis. Antibiotic susceptibility test showed the organism to be most susceptible to Streptomycin, which was administered via drinking water at dosage of 40mg/kg with good recovery of the flock. It was concluded that the occurrence of Salmonella Enteritidis infection in this flock might be from the hatchery or via ingestion of contaminated feed and water. Day old chicks should be screened for Salmonella infection and strict biosecurity should be instituted on poultry farms. Keywords: Mortality, Necrosis, Pullets, Salmonella Enteritidis, streptomycin

scholarly journals Rapid Antibiotic Susceptibility Testing of Tier-1 Agents Bacillus anthracis, Yersinia pestis, and Francisella tularensis Directly From Whole Blood Samples

2021 ◽  
Vol 12 ◽  
Author(s):  
Shahar Rotem ◽  
Ohad Shifman ◽  
Moshe Aftalion ◽  
David Gur ◽  
Tamar Aminov ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Yersinia Pestis ◽  
Francisella Tularensis ◽  
Whole Blood ◽  
Antibiotic Susceptibility ◽  
Blood Samples ◽  
Wide Range ◽  
Tier 1 ◽  
Whole Blood Samples ◽  
Susceptibility Tests

Rapid antibiotic susceptibility tests, performed directly on whole blood samples, will offer great clinical advantages. This issue is of considerable importance when it comes to bioterror pathogens where prompt antibiotic treatment should be offered to infected patients as well as prophylaxis to suspected exposed individuals. Herein, we describe a novel and rapid method, named MAPt, that is based on the direct application of a blood sample onto solid agar that has been embedded with different concentrations of the tested antibiotic. Following a short incubation, bacterial growth is monitored by qPCR. The method was applied on blood cultures and whole blood samples inoculated with the Tier-1 pathogens Bacillus anthracis, Yersinia pestis, and Francisella tularensis. The use of agar medium, which better supports the growth of bacteria at low concentrations, together with the use of qPCR, which provides sensitivity and specificity, allowed minimal inhibitory concentration (MIC) determination to a wide range of bacterial concentrations, ranging from ∼5 × 102 cfu/ml up to 108 cfu/ml. The omission of the enrichment procedure in blood culture and the isolation step, both required in standard antibiotic susceptibility tests (ASTs), allowed a dramatic reduction in time to answer, from a few days to a few hours. The total time required for MIC determination was ∼6 h for fast-growing bacteria, such as B. anthracis, and 12–16 h for slow-growing bacteria, represented by Y. pestis and F. tularensis. Accordingly, MAPt may offer health authorities means for public preparedness in the case of a bioterror attack as well as prompt clinical treatment options in common blood stream infections.

Epidemiology, risk factors and antimicrobial resistance of Escherichia coli bacteremia

2021 ◽  
Vol 11 (Number 2) ◽  
pp. 58-67
Author(s):  
Mahjuba Umme Salam ◽  
Selina Yasmin ◽  
Md. Rashedul Haque ◽  
Sharmin Ahmed ◽  
Shahidul Alam ◽  
...  
Keyword(s):  
Risk Factors ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antibiotic Susceptibility ◽  
Antimicrobial Agents ◽  
Blood Stream Infection ◽  
Incidence Rates ◽  
Diffusion Method ◽  
Antibiotic Susceptibility Test ◽  
Multiple Drugs

Background: Escherichia coli is a common causative of blood stream infection having potentials to produce significant morbidity and mortality. This organism also has the ability to develop resistance against antimicrobial agents. Knowing its epidemiology, risk factors and antimicrobial resistance patterns can help preventing and managing bacteremia caused by this organism. Materials and methods: This was across sectional observational study carried out from February 2017 to February 2018 on 64 blood culture positive Escherichia coli infected patients admitted in Medicine inpatient of a medical college hospital. Age, sex, mode of acquisition of infection, history of prior empiric antibiotic treatment, duration of hospital stay, development of complication were observed and noted. Antibiotic susceptibility test for all isolates was performed by Kirby-Bauer disc diffusion method. Predesigned semi-structured data collection from was used and collected data were analyzed manually and expressed in descriptive statistical terms. Results: Of the 64 enrolled patients, 47(73.43%) were female. Average age of affection was 53.48±20.65 years and increased incidence rates (51.56%) was observed at age >60 years. Infection was communityacquired in 35.84% cases and urinary tract infection was the most frequent (46.3) risk factor. More than eighty seven percent of samples showed resistance to at least one antimicrobial agent and resistance to multiple drugs was associated with complications. Conclusion: Escherichia coli bacteremia has high incidence rates for antimicrobial resistance and mortality. Continuous surveillance and antibiotic susceptibility pattern monitoring is essential to develop regional antibiotic therapy protocols.

scholarly journals Susceptibility of Virulent Yersinia pestis Bacteria to Predator Bacteria in the Lungs of Mice

2018 ◽  
Vol 7 (1) ◽  
pp. 2 ◽  
Author(s):  
Riccardo Russo ◽  
Irina Kolesnikova ◽  
Thomas Kim ◽  
Shilpi Gupta ◽  
Androulla Pericleous ◽  
...  
Keyword(s):  
Yersinia Pestis ◽  
Bacterial Infections ◽  
Treatment Modalities ◽  
The Novel ◽  
Global Public Health ◽  
Antibiotic Resistant ◽  
Colony Forming Units ◽  
Tier 1 ◽  
Predatory Bacteria ◽  
Lethal Doses

Multi-drug resistant bacterial infections are a serious threat to global public health. Changes in treatment modalities and prudent use of antibiotics can assist in reducing the threat, but new approaches are also required for untreatable cases. The use of predatory bacteria, such as Bdellovibrio bacteriovorus, is among the novel approaches being considered as possible therapeutics for antibiotic resistant and/or unidentified bacterial infections. Previous studies have examined the feasibility of using predatory bacteria to reduce colony-forming units (CFUs) in the lungs of rats exposed to lethal doses of Klebsiella pneumoniae; here we apply the approach to the Tier 1 select agent Yersinia pestis, and show that three doses of B. bacteriovorus introduced every six hours reduces the number of CFUs of Y. pestis in the lungs of inoculated mice by 86% after 24 h of infection. These experiments further demonstrate that predatory bacteria may serve to combat Gram negative bacterial infections, including those considered potential bioweapon agents, in the future.

scholarly journals Improve antibiotic susceptibility test and its quality control by linear-gradient plate

2017 ◽  
Vol 47 (7) ◽  
pp. 740-749
Author(s):  
Qing ZHANG ◽  
Yin ZHANG ◽  
Jing QI ◽  
YanBo LUO ◽  
LuLu LI ◽  
...  
Keyword(s):  
Quality Control ◽  
Antibiotic Susceptibility ◽  
Susceptibility Test ◽  
Linear Gradient ◽  
Antibiotic Susceptibility Test ◽  
Gradient Plate
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