scholarly journals Development and Comparison of a Panel of Modified CS17 Fimbrial Tip Adhesin Proteins as Components for an Adhesin-Based Vaccine against Enterotoxigenic Escherichia coli

2021 ◽  
Vol 9 (8) ◽  
pp. 1646
Author(s):  
Yang Liu ◽  
Milton Maciel ◽  
Aisling O’Dowd ◽  
Steven T. Poole ◽  
Julianne E. Rollenhagen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Thermal Stability ◽  
Vaccine Candidate ◽  
Enterotoxigenic Escherichia Coli ◽  
Vaccine Candidates ◽  
Resource Limited ◽  
Significant Difference ◽  
Colonization Factors ◽  
New Generation ◽  
Adhesin Proteins

Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in travelers and children in resource-limited countries. ETEC colonization factors, fimbrial tip adhesins and enterotoxins are key virulence factors, and thus have been studied as vaccine candidates. Some prevalent colonization factors, including CFA/I and CS17, belong to the class 5 family. We previously found that passive oral administration of hyperimmune bovine colostral IgG (bIgG) raised against dscCfaE (donor strand complemented CFA/I tip adhesin) protected volunteers against CFA/I+ ETEC challenge, while anti-dscCsbD bIgG (CS17 tip adhesin) did not confer protection. These findings led us to develop and optimize a panel of alternative CsbD-based vaccine candidates based on allele matching and in silico protein engineering. Physicochemical characterizations revealed that an optimized vaccine candidate dscCsbDLSN139(P218A/G3) had the greatest thermal stability among the six tested dscCsbD adhesins, whereas the overall secondary structures and solubility of these adhesins had no obvious differences. Importantly, dscCsbDLSN139(P218A/G3) elicited significantly higher CS17+ ETEC hemagglutination inhibition titers in sera from mice intranasally immunized with the panel of dscCsbD adhesins, while no significant difference was observed among heterologous neutralizing titers. Our results strongly advocate for the incorporation of these modifications into a new generation of CsbD-based ETEC vaccine candidates.

Over-expression of major colonization factors of enterotoxigenic Escherichia coli, alone or together, on non-toxigenic E. coli bacteria

Vaccine ◽  
2010 ◽  
Vol 28 (43) ◽  
pp. 6977-6984 ◽  
Author(s):  
Joshua Tobias ◽  
Jan Holmgren ◽  
Maria Hellman ◽  
Erik Nygren ◽  
Michael Lebens ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Over Expression ◽  
E Coli ◽  
Colonization Factors

scholarly journals Colonization factors associated with enterotoxigenic Escherichia coli isolated in Thailand.

1984 ◽  
Vol 45 (2) ◽  
pp. 525-527 ◽  
Author(s):  
S Changchawalit ◽  
P Echeverria ◽  
D N Taylor ◽  
U Leksomboon ◽  
C Tirapat ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Factors Associated ◽  
Colonization Factors

Prevalence of Toxin Types and Colonization Factors in Enterotoxigenic Escherichia coli Isolated during a 2-Year Period from Diarrheal Patients in Bangladesh

2000 ◽  
Vol 38 (1) ◽  
pp. 27-31
Author(s):  
Firdausi Qadri ◽  
Swadesh Kumar Das ◽  
A. S. G. Faruque ◽  
George J. Fuchs ◽  
M. John Albert ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Severe Disease ◽  
Surface Antigens ◽  
Enterotoxigenic Escherichia Coli ◽  
Colonization Factor ◽  
Heat Stable ◽  
Routine Surveillance ◽  
Stool Samples ◽  
Colonization Factors ◽  
Severity Of The Disease

ABSTRACT The prevalence of toxin types and colonization factors (CFs) of enterotoxigenic Escherichia coli (ETEC) was prospectively studied with fresh samples ( n = 4,662) obtained from a 2% routine surveillance of diarrheal stool samples over 2 years, from September 1996 to August 1998. Stool samples were tested by enzyme-linked immunoassay techniques and with specific monoclonal antibodies for the toxins and CFs. The prevalence of ETEC was 14% ( n = 662), with over 70% of the strains isolated from children 0 to 5 years of age, of whom 93% were in the 0- to 3-year-old age range. Of the total ETEC isolates, 49.4% were positive for the heat-stable toxin (ST), 25.4% were positive for the heat-labile toxin (LT) only, and 25.2% were positive for both LT and ST. The rate of ETEC isolation peaked in the hot summer months of May to September and decreased in winter. About 56% of the samples were positive for 1 or more of the 12 CFs that were screened for. The coli surface antigens CS4, CS5, and/or CS6 of the colonization factor antigen (CFA)/IV complex were most prevalent (incidence, 31%), followed by CFA/I (23.5%) and coli surface antigens CS1, CS2, and CS3 of CFA/II (21%). In addition, other CFs detected in decreasing order were CS7 (8%), CS14 (PCFO166) (7%), CS12 (PCFO159) (4%), CS17 (3%), and CS8 (CFA/III) (2.7%). The ST- or LT- and ST-positive ETEC isolates expressed the CFs known to be the most prevalent (i.e., CFA/I, CFA/II, and CFA/IV), while the strains positive for LT only did not. Among children who were infected with ETEC as the single pathogen, a trend of relatively more severe disease in children infected with ST-positive ( P < 0.001) or LT- and ST-positive ( P < 0.001) ETEC isolates compared to the severity of the disease in children infected with LT only-positive ETEC isolates was seen. This study supports the fact that ETEC is still a major cause of childhood diarrhea in Bangladesh, especially in children up to 3 years of age, and that measures to prevent such infections are needed in developing countries.

Protective immunity of a Multivalent Vaccine Candidate against piglet diarrhea caused by enterotoxigenic Escherichia coli (ETEC) in a pig model

Vaccine ◽  
2018 ◽  
Vol 36 (5) ◽  
pp. 723-728 ◽  
Author(s):  
Henghui Zhang ◽  
Yongping Xu ◽  
Zhijun Zhang ◽  
Jiansong You ◽  
Yanyong Yang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protective Immunity ◽  
Vaccine Candidate ◽  
Pig Model ◽  
Enterotoxigenic Escherichia Coli ◽  
Multivalent Vaccine

Identification of epitope-based peptide vaccine candidates against enterotoxigenic Escherichia coli: a comparative genomics and immunoinformatics approach

2016 ◽  
Vol 12 (3) ◽  
pp. 890-901 ◽  
Author(s):  
Kusum Mehla ◽  
Jayashree Ramana
Keyword(s):  
Escherichia Coli ◽  
Comparative Genomics ◽  
Peptide Vaccine ◽  
Enterotoxigenic Escherichia Coli ◽  
Vaccine Candidates ◽  
Integrated Omics ◽  
Potential Vaccine

In the present study, we have employed integrated omics approach to identify potential vaccine candidates against ETEC.

Enterotoxigenic Escherichia coli Isolated from Foods in São Paulo, Brazil

1987 ◽  
Vol 50 (10) ◽  
pp. 832-834 ◽  
Author(s):  
BERNADETTE D. G. M. FRANCO ◽  
BEATRIZ E. C. GUTH ◽  
LUIZ R. TRABULSI
Keyword(s):  
Escherichia Coli ◽  
Food Samples ◽  
Sao Paulo ◽  
Enterotoxigenic Escherichia Coli ◽  
São Paulo ◽  
E Coli ◽  
Colonization Factors ◽  
The City

Incidence of enterotoxigenic Escherichia coli (ETEC) in foods usually consumed in the city of Sao Paulo, Brazil was determined. Raw and cooked foods of animal and vegetable origin were investigated. Enterotoxigenic strains were found in approximately 3.5% of food samples contaminated with E. coli. There was a great predominance of ETEC strains producing only LT enterotoxin. None of the isolated strains produced LT and ST simultaneously. Several serotypes were involved, and none of them was positive for colonization factors CFA-I and CFA-II. One ETEC showed resistance to some antibiotics but most were sensitive to the ones tested.

scholarly journals The Major Subunit, CfaB, of Colonization Factor Antigen I from Enterotoxigenic Escherichia coli Is a Glycosphingolipid Binding Protein

2006 ◽  
Vol 74 (6) ◽  
pp. 3488-3497 ◽  
Author(s):  
Lena Jansson ◽  
Joshua Tobias ◽  
Michael Lebens ◽  
Ann-Mari Svennerholm ◽  
Susann Teneberg
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Binding Capacity ◽  
Enterotoxigenic Escherichia Coli ◽  
E Coli ◽  
Colonization Factor ◽  
Mucosal Surfaces ◽  
Colonization Factors ◽  
Bacteria Adhesion ◽  
Colonization Factor Antigen I

ABSTRACT Bacterial adherence to mucosal surfaces is an important virulence trait of pathogenic bacteria. Adhesion of enterotoxigenic Escherichia coli (ETEC) to the intestine is mediated by a number of antigenically distinct colonization factors (CFs). One of the most common CFs is CFA/I. This has a fimbrial structure composed of a major repeating subunit, CfaB, and a single tip subunit, CfaE. The potential carbohydrate recognition by CFA/I was investigated by binding CFA/I-fimbriated bacteria and purified CFA/I fimbriae to a large number of variant glycosphingolipids separated on thin-layer chromatograms. For both fimbriated bacteria and purified fimbriae, specific interactions could be identified with a number of nonacid glycosphingolipids. These included glucosylceramide, lactosylceramide with phytosphingosine and/or hydroxy fatty acids, neolactotetraosylceramide, gangliotriaosylceramide, gangliotetraosylceramide, the H5 type 2 pentaglycosylceramide, the Lea-5 glycosphingolipid, the Lex-5 glycosphingolipid, and the Ley-6 glycosphingolipid. These glycosphingolipids were also recognized by recombinant E. coli expressing CFA/I in the absence of tip protein CfaE, as well as by purified fimbriae from the same strain. This demonstrates that the glycosphingolipid-binding capacity of CFA/I resides in the major CfaB subunit.

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