scholarly journals Differences between the Leaf Mycobiome of Coffea arabica and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content

2021 ◽  
Vol 9 (11) ◽  
pp. 2296
Author(s):  
Leandro Pio de Sousa ◽  
Oliveiro Guerreiro Filho ◽  
Jorge Maurício Costa Mondego

The study of microbes associated with the coffee tree has been gaining strength in recent years. In this work, we compared the leaf mycobiome of the traditional crop Coffea arabica with wild species Coffea racemosa and Coffea stenophylla using ITS sequencing for qualitative information and real-time PCR for quantitative information, seeking to relate the mycobiomes with the content of caffeine and chlorogenic acid in leaves. Dothideomycetes, Wallemiomycetes, and Tremellomycetes are the dominant classes of fungi. The core leaf mycobiome among the three Coffea species is formed by Hannaella, Cladosporium, Cryptococcus, Erythrobasidium, and Alternaria. A network analysis showed that Phoma, an important C. arabica pathogen, is negatively related to six fungal species present in C. racemosa and C. stenophylla and absent in C. arabica. Finally, C. arabica have more than 35 times the concentration of caffeine and 2.5 times the concentration of chlorogenic acid than C. stenophylla and C. racemosa. The relationship between caffeine/chlorogenic acid content, the leaf mycobiome, and genotype pathogen resistance is discussed.

1976 ◽  
Vol 56 (1) ◽  
pp. 157-160 ◽  
Author(s):  
J. W. SHEPPARD ◽  
J. F. PETERSON

Chlorogenic acid, which occurs in high concentrations in tobacco, has been suggested as a factor in the resistance of potatoes to both verticillium wilt and common scab. In an investigation of the relationship between chlorogenic acid content and the resistance of tobacco (Nicotiana tabacum L.) cultivars to wilt caused by Verticillium dahliae Kleb., a simple method was devised for the estimation of relative chlorogenic acid content. Results obtained suggested a possible relationship or correlation between content of chlorogenic acid and resistance of tobacco plants to verticillium wilt, and that an estimation of chlorogenic acid content might be useful when screening tobacco varieties for verticillium resistance, if accompanied by appropriate testing for susceptibility to virus infection.


2019 ◽  
Vol 13 (5) ◽  
pp. 58-63
Author(s):  
Tadesse Dado Adane ◽  
Alresawum Asresahegn Yoseph ◽  
Gudishe Goroya Kusse

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2040
Author(s):  
Chia-Fang Tsai ◽  
Irvan Prawira Julius Jioe

Coffee is one of the main economic crops in the world and is now widely grown throughout Taiwan. The process of roasting coffee begins with the heating and smooth expansion of raw beans, which leads to changes in appearance and color while affecting the flavor and taste of coffee. So far, most coffee manufacturers have used visual inspection or colorimeter methods to identify differences in coffee quality. Moreover, there is no literature discussing the correlation of roasted bean color with caffeine and chlorogenic acid content. Therefore, the purpose of this experiment was to analyze the chlorogenic acid and caffeine content and their correlation with bean color under different roasting degrees and from different sources to establish basic data for the rapid identification of coffee quality in the future. In this experiment, the coffee Coffea arabica typica from Dongshan, Gukeng, and Sumatra’s Indonesian rainforest was used, and the beans were roasted into four degrees: raw bean, light, medium, and dark roast, to investigate the appearance of the coffee beans and its correlation with caffeine and chlorogenic acid content. The results showed that with a higher roasting degree, caffeine content increased gradually, except for Indonesian beans, but the chlorogenic acid content in all samples showed a declining trend with the increase in roasting degree. The correlation between the chlorogenic acid content and the color space value of the coffee bean color shows that L*, a*, and h° in both ground and unground coffee are highly correlated. The C* value of the ground and unground coffee showed a correlation coefficient of r = 0.159 ns and 0.299 ns, respectively. The correlation between the caffeine content and the color space value of the unground coffee bean shows that the a*, b*, and C* value is highly correlated with the caffeine content. The color space values of ground coffee beans show no correlation with caffeine.


2017 ◽  
Vol 66 (4/5) ◽  
pp. 266-281
Author(s):  
Nafisat Toyin Adewale ◽  
Yushiana Mansor ◽  
Muhammad-Bashir Owolabi Yusuf ◽  
Ahmeed Onikosi

Purpose This study investigates the moderating effects of age, experience and educational qualification on the relationship between uncertainty and subjective task complexity among lawyers working in private law firms in Lagos State, Nigeria. Design/methodology/approach A survey method was adopted and data were gathered using questionnaires. The analysis was carried out based on partial least squares structural equation modelling using SmartPLS 2.0 M3 software. Findings Results showed that the effect of uncertainty on subjective task complexity is significantly moderated by age, educational attainment, experience of the lawyers under study. Research limitations/implications Although data were collected in the most populated state and commercial hub of Nigeria, generalisation based on findings may still need to be made with caution. Practical implications Attainment of higher educational qualification is highly important for lawyers even though the minimum requirement to practice as a lawyer is a degree. Lawyers with higher degrees (LLM and PhD) had less uncertainty and perceived their tasks to be less complex compared to their counterparts who had the first degree (LLB). Originality/value The demographic profile of professionals (age, education and experience) has proven to have an impact on their perception about task complexity as determined by uncertainty as found in this study.


1976 ◽  
Vol 56 (4) ◽  
pp. 901-905 ◽  
Author(s):  
D. G. DORRELL

The effect of seeding date on the chlorogenic acid content of sunflower seed flour was determined by seeding the cultivars Krasnodarets and Peredovik at seven dates, starting on 14 May, over 3 yr. Sequential plantings were made at increments of approximately 70 growing degree days (base = 5.6 C). Plants were harvested at normal field maturity. The time and rate of deposition of chlorogenic acid was determined by harvesting plants at 7-day intervals from 21 to 49 days after flowering. The seeds were dehulled and defatted before determining the chlorogenic acid content of the flour. Chlorogenic acid content declined steadily from an average of 4.22% for the first seeding to 3.30% for the last seeding. About one-half of the total chlorogenic acid was present 21 days after flowering. Deposition continued rapidly for the next 14 days then the level began to stabilize. Delay in seeding tended to shorten the period of vegetative growth and shift the deposition of chlorogenic acid to a cooler portion of the growing season. It is suggested that a combination of these factors caused the reduction in chlorogenic acid content of sunflower flour.


2021 ◽  
Vol 12 ◽  
Author(s):  
Shuguang Zhao ◽  
Yiming Liu

This study examines the relationship between cognitive and affective factors and people's information-seeking and -avoiding behaviours in acute risks with a 1,946-sample online survey conducted in February 2020, during the outbreak of the COVID-19 pandemic in mainland China. Multiple linear regression analysis showed that perceived information insufficiency correlates negatively with information-seeking behaviour and there was an inverted U-shaped relationship between information insufficiency and avoidance behaviour. As for the risk-related cognitive factors, information seeking increases as perceived severity of risks rises, while information avoiding increases as perceived susceptibility rises. Perceived response efficacy positively correlates with information-seeking and negatively with information-avoidance behaviours. Preliminary results also indicated that different affective factors relate to information-seeking and avoidance behaviours differently.


2012 ◽  
Vol 32 (3) ◽  
pp. 179-186 ◽  
Author(s):  
Mohammad Murtaza Mehdi ◽  
Prabhakar Singh ◽  
Syed Ibrahim Rizvi

Sialic acids are substituted neuraminic acid derivatives which are typically found at the outermost end of glycan chains on the membrane in all cell types. The role of erythrocyte membrane sialic acids during aging has been established however the relationship between sialic acid and oxidative stress is not fully understood. The present work was undertaken to analyze the relationship between erythrocyte membrane sialic acid with its plasma level, membrane and plasma lipid hydroperoxide levels and plasma total antioxidant capacity. Results show that sialic acid content decreases significantly (P< 0.001) in RBC membrane (r= −0.901) and increases in plasma (r= 0.860) as a function of age in humans. Lipid peroxidation measured in the form of hydroperoxides increases significantly (P< 0.001) in plasma (r= 0.830) and RBC membranes (r= 0.875) with age in humans. The Trolox Equivalent Total Antioxidant Capacity (TETAC) of plasma was found to be significantly decreased (P< 0.001,r= −0.844). We observe significant correlations between decrease of erythrocyte membrane sialic acid and plasma lipid hydroperoxide and TETAC. Based on the observed correlations, we hypothesize that increase in oxidative stress during aging may influence the sialic acid decomposition from membrane thereby altering the membrane configuration affecting many enzymatic and transporter activities. Considering the importance of plasma sialic acid as a diagnostic parameter, it is important to establish age-dependent reference.


2018 ◽  
Vol 18 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Dinara Abdikhaliyevna Aldibekova ◽  
Murat Kizaibek ◽  
Mahaya Aisijiang ◽  
Gulzhamal Dyuskaliyeva ◽  
Angsar Taldau ◽  
...  

Author(s):  
J. lavsky ◽  
G.G. Long ◽  
A.J. Allen ◽  
L. Leblanc ◽  
M. Prystay ◽  
...  

Abstract The microstructure of plasma-sprayed deposits (PSD) is dominated by two void systems - interlamellar pores and intralamellar cracks - each with a different anisotropy. Varying anisotropics and crack-to-pore ratios within PSDs are responsible for the anisotropic properties observed in the deposits. While it is difficult to apply standard porosity measurement techniques to the assessment of anisotropic microstructures, novel techniques utilizing different approaches have recently emerged. Image analysis (IA) of impregnated PSD samples is the most direct technique. The structure is stabilized by impregnation and then polished and imaged. The limitations of IA lie in the impregnation process and in the subsequent polishing. Also, the images produced from anisotropic materials can be difficult to interpret quantitatively. The technique of small-angle neutron scattering (SANS) has recently been successfully applied to the study of PSDs. The major advantages of SANS are that it does not require sample preparation and that quantitative information can be gotten about the separate crack and pore systems, including their distinctive anisotropics. However, the relationship between the SANS results and the underlying structure is more complex and less intuitive than for IA, and the availability of the SANS technique is limited by the need to have access to a powerful neutron source, such as a reactor. Also, the two techniques present different views of the microstructure because of the different sensitivities in different parts of the size range. This paper compares results from IA and SANS from a set of thick plasma-sprayed ceramic deposits possessing a range of crack/pore microstructures, and discusses how the two techniques might complement one another.


Sign in / Sign up

Export Citation Format

Share Document