scholarly journals The Analysis of Chlorogenic Acid and Caffeine Content and Its Correlation with Coffee Bean Color under Different Roasting Degree and Sources of Coffee (Coffea arabica Typica)

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2040
Author(s):  
Chia-Fang Tsai ◽  
Irvan Prawira Julius Jioe
Keyword(s):  
Chlorogenic Acid ◽  
Acid Content ◽  
Coffea Arabica ◽  
Color Space ◽  
Rapid Identification ◽  
Coffee Bean ◽  
Caffeine Content ◽  
Coffee Beans ◽  
C Value ◽  
Highly Correlated

Coffee is one of the main economic crops in the world and is now widely grown throughout Taiwan. The process of roasting coffee begins with the heating and smooth expansion of raw beans, which leads to changes in appearance and color while affecting the flavor and taste of coffee. So far, most coffee manufacturers have used visual inspection or colorimeter methods to identify differences in coffee quality. Moreover, there is no literature discussing the correlation of roasted bean color with caffeine and chlorogenic acid content. Therefore, the purpose of this experiment was to analyze the chlorogenic acid and caffeine content and their correlation with bean color under different roasting degrees and from different sources to establish basic data for the rapid identification of coffee quality in the future. In this experiment, the coffee Coffea arabica typica from Dongshan, Gukeng, and Sumatra’s Indonesian rainforest was used, and the beans were roasted into four degrees: raw bean, light, medium, and dark roast, to investigate the appearance of the coffee beans and its correlation with caffeine and chlorogenic acid content. The results showed that with a higher roasting degree, caffeine content increased gradually, except for Indonesian beans, but the chlorogenic acid content in all samples showed a declining trend with the increase in roasting degree. The correlation between the chlorogenic acid content and the color space value of the coffee bean color shows that L*, a*, and h° in both ground and unground coffee are highly correlated. The C* value of the ground and unground coffee showed a correlation coefficient of r = 0.159 ns and 0.299 ns, respectively. The correlation between the caffeine content and the color space value of the unground coffee bean shows that the a*, b*, and C* value is highly correlated with the caffeine content. The color space values of ground coffee beans show no correlation with caffeine.

scholarly journals Differences between the Leaf Mycobiome of Coffea arabica and Wild Coffee Species and Their Modulation by Caffeine/Chlorogenic Acid Content

2021 ◽  
Vol 9 (11) ◽  
pp. 2296
Author(s):  
Leandro Pio de Sousa ◽  
Oliveiro Guerreiro Filho ◽  
Jorge Maurício Costa Mondego
Keyword(s):  
Chlorogenic Acid ◽  
Acid Content ◽  
Information Seeking ◽  
Coffea Arabica ◽  
Pathogen Resistance ◽  
Quantitative Information ◽  
Fungal Species ◽  
Its Sequencing ◽  
Wild Coffee ◽  
The Relationship

The study of microbes associated with the coffee tree has been gaining strength in recent years. In this work, we compared the leaf mycobiome of the traditional crop Coffea arabica with wild species Coffea racemosa and Coffea stenophylla using ITS sequencing for qualitative information and real-time PCR for quantitative information, seeking to relate the mycobiomes with the content of caffeine and chlorogenic acid in leaves. Dothideomycetes, Wallemiomycetes, and Tremellomycetes are the dominant classes of fungi. The core leaf mycobiome among the three Coffea species is formed by Hannaella, Cladosporium, Cryptococcus, Erythrobasidium, and Alternaria. A network analysis showed that Phoma, an important C. arabica pathogen, is negatively related to six fungal species present in C. racemosa and C. stenophylla and absent in C. arabica. Finally, C. arabica have more than 35 times the concentration of caffeine and 2.5 times the concentration of chlorogenic acid than C. stenophylla and C. racemosa. The relationship between caffeine/chlorogenic acid content, the leaf mycobiome, and genotype pathogen resistance is discussed.

Composition of amino acids and fatty acids on Luwak coffee processing

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 60-64
Author(s):  
Fitri ◽  
A. Laga ◽  
Z. Dwyana ◽  
A.B. Tawali
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Amino Acid ◽  
Acid Content ◽  
Green Bean ◽  
Total Amino Acid ◽  
Coffee Bean ◽  
Roasted Coffee ◽  
Coffee Beans

The processing carried out on coffee beans such as fermentation and roasting can affect the contents of amino and fatty acids of coffee beans. This study aimed to determine the amount of amino acid and fatty acid content in Luwak coffee bean during processing. The amino acids of coffee were analyzed using HPLC, while fatty acids of coffee were analyzed using GC-MS. The results showed a change in total amino acid content in raw coffee bean (3.04%), green bean coffee (6.93%), and roasted coffee (6.83%). The total fatty acid of raw coffee bean (1199.86 mg/100 g), green bean coffee (3147.56 mg/100 g), and roasted coffee (6282.4 mg/100 g) also experienced significant changes

scholarly journals Changes in caffeine of fermented Liberica coffee beans cv. Liberoid Meranti on roasting levels

2021 ◽  
Vol 306 ◽  
pp. 01026
Author(s):  
Nendyo Adhi Wibowo ◽  
Wibowo Mangunwardoyo ◽  
Tri Joko Santoso ◽  
Yasman
Keyword(s):  
Coffee Bean ◽  
Caffeine Content ◽  
Statistical Application ◽  
Coffee Beans ◽  
Significant Difference ◽  
Wet Processing ◽  
Hydrolytic Bacteria ◽  
Compound Process ◽  
Roasting Temperature ◽  
Post Hoc

One of the varieties of Liberica coffee in Indonesia is the Liberoid Meranti coffee. Fermentation technology and the roasting temperature of Liberica coffee are needed in changing the composition of caffeine in coffee beans. The aim of this research was to see the caffeine content of the Liberica coffee beans cv. Liberoid Meranti fermented by hydrolytic bacteria on levels at a light, medium, and dark roasting temperatures. Coffee fermentation was carried out using the wet processing method and a roasting temperature of 150oC, 175oC, 200oC, and caffeine analysis was carried out by UV-Visible spectrophotometry. The data obtained from each parameter based on roasting levels were processed using the statistical application of Minitab version 16 for Windows and analyzed using ANOVA with a level of 5%. If there is a significant difference from the F-test result (p<0.05), then proceed with the post hoc test. The results showed that all samples contain large amounts of caffeine with various concentrations. The level concentration of caffeine in light roast samples in the range of 10,80 ppm-12,52 ppm, medium roast samples in the range of 11,46 ppm-14,08 ppm, and dark roast samples in the range of 14,49 ppm-16,94 ppm. The caffeine content in fermented Liberica coffee bean experienced a significant difference. The fermentation and roasting process of coffee beans is a complex compound process that can cause changes in caffeine levels.

scholarly journals The Effect of Orlistat, Green Coffee Bean Extract, and Its Combinations on Lipid Profi le and Adiponectin Levels

Jurnal NERS ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 26 ◽  
Author(s):  
Joko Setyono ◽  
Dwi Adi Nugroho ◽  
Mustofa Mustofa ◽  
Saryono Saryono
Keyword(s):  
Chlorogenic Acid ◽  
Water Extract ◽  
Ethanol Extract ◽  
Control Group ◽  
Coffee Bean ◽  
Green Coffee ◽  
Green Coffee Beans ◽  
Coffee Beans ◽  
Significant Difference ◽  
Obese Rats

Introduction: Obesity prevalence is estimated increases, reached 19.1% of the population aged 15 years and over. This study aimed to determine the differences of the anti- obesity effect of orlistat, an extract of green coffee beans (Coffea canephora robusta), and its combination to the adiponectin levels and lipid profi le. Method: This research was true experimental post -test only with control group design with completely randomized design (CRD). Experimental animals (Rattus novergicus) were divided into 6 group, group 1 ( negative control ), group 2 ( positive control ), group 3 was group of obese rats fed orlistat dose of 15.9 mg/kg, group 4 was the group of obese rats were fed ethanol extract of green coffee beans dose of 400 mg/kg, group 5 was the group of obese rats were given water extract of green coffee beans dose of 400 mg/kg, and group 6 was group of obese rats were fed a combination of orlistat dose of 15.9 mg/kgand ethanol extract of green coffee beans at a dose of 400 mg/kg. Lipid profi les and adiponectin levels were measured with a spectrophotometer at 500nm absorbance. The data were analyzed by one-way ANOVA, and then post hoc Least Significant Difference (LSD) with α = 0.05. Result: Ethanol extract of green coffee is more effi cient in lowering LDL cholesterol, increasing HDL cholesterol, and lowering the total cholesterol levels on HFD diet-induced mice, but there was no difference in lowering triglycerides . The combination of ethanol extract of green coffee with orlistat showedthe increasing of adiponectin levels were highest than the other treatment groups. Discussion: The ethanol extract of green coffee readily diffuses through the digestive tract epithelium. Green coffee contains chlorogenic acid active compounds that can increase the body’s metabolism, increase fatty acid oxidation, reduce levels of triglycerides in the liver, and working to inhibit lipase and amylase pancreaticenzymes. In addition to chlorogenic acid, polyphenol content in coffee is also potentially reduce visceral fat accumulation. Preparations extract by ethanol allows the absorption process is done effi ciently and quickly.Keywords: obesity, orlistat, greencoffee, lipid profi le, adiponectin

scholarly journals Kadar Asam Klorogenat (CGA) Dalam Biji Kopi Arabika (Coffea Arabica) Asal Wamena, Papua

2015 ◽  
Vol 3 (2) ◽  
pp. 313
Author(s):  
Septiani Mangiwa ◽  
Alowisya Futwembun ◽  
Puteri M. Awak
Keyword(s):  
Chlorogenic Acid ◽  
Flow Rate ◽  
Chemical Compound ◽  
Coffea Arabica ◽  
Polyphenolic Compounds ◽  
Arabica Coffee ◽  
Injection Volume ◽  
Coffee Beans ◽  
Eluent Flow Rate ◽  
Roasting Temperature

Chlorogenic acid (CGA) is one of chemical compound in coffee and class of polyphenolic compounds that have activity as antioxidant.  This study aims to determine the CGA contain in Arabica coffee beans from Wamena regency of Jayawijaya, Papua.  Coffee beans  were roasted by three roasting temperature i.e. 75, 150 and 2250 C while unroasted one was used as controls. Extraction was performed by soxhletation using metanol as  a solvent for 5 hours. Separation and  CGAcontent were determined using HPLC uses C-18 column, 150 mm length, 4,6 mm in diameter, with metanol-fosfat buffer 10 mM pH 2,6(30:70) as eluent, flow rate of 1 mL/minutes, injection volume of 20 µL and measured by spectrophotometer UV-Vis in 329 nm. The result of the study shows the CGAcontent  in treated beans with temperature roasting of 75, 150 and 2250C  respectively were 6,93 ; 9,33 and 7,12 % while the unroasted one was  7,73 %. The lowest CGA content was found in coffee beans roasted in 750C while the highest in 1500 C.

scholarly journals The Influence of Strata on the Nutrient Recycling within a Tropical Certified Organic Coffee Production System

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
F. Mamani-Pati ◽  
D. E. Clay ◽  
S. A. Clay ◽  
H. Smeltekop ◽  
M. A. Yujra-Callata
Keyword(s):  
Coffea Arabica ◽  
Nutrient Recycling ◽  
Coffee Bean ◽  
Coffee Production ◽  
Coffee Plantations ◽  
Green Coffee Beans ◽  
Coffee Beans ◽  
Certified Organic ◽  
Middle Strata ◽  
Organic Coffee

In tropical Bolivia coffee plantations, the plant community can be separated into high (trees), middle (coffee), and low (weed) strata. Understanding the importance of each stratum is critical for improving the sustainability of the system. The objective of this study was to determine the importance of strata on nutrient recycling. Litter falls from the upper and middle strata were collected monthly using cone-shaped traps and divided by species into leaves, branches, flowers, and fruits. Dry biomass additions to the soil from high and middle strata totaled 12,655 kg (ha yr)−1 annually. About 76% of the biomass was provided by plants of the genus Inga (I. adenophylla and I. oerstediana). The middle stratum (Coffea arabica L.) provided 24% litterfall biomass. This stratum also produced 1,800 kg coffee bean per ha (12% moisture) which sold for $2.94 kg−1. In the lower stratum, Oxalis mollissima returned 36 kg N ha−1, while Solanum nodiflorum returned 49 kg K ha−1, and Urtica sp. returned 18 kg Ca ha−1. The nutrients recycled through plants in three strata exceeded the amount of nutrients removed in green coffee beans.

scholarly journals Determining the Efficiency and Yield of Caffeine Extraction for Robusta and Arabica Coffee Beans

2021 ◽  
Vol 9 (3) ◽  
Author(s):  
Zeyu Yu ◽  
Akshay Jakkidi Reddy ◽  
Himanshu Wagh
Keyword(s):  
Coffee Bean ◽  
Caffeine Consumption ◽  
Vacuum Filtration ◽  
Arabica Coffee ◽  
Robusta Coffee ◽  
Caffeine Content ◽  
Coffee Beans ◽  
The Difference ◽  
Solid Form ◽  
Source Of Error

The objective of this review is to determine the difference in caffeine content in the coffee beans from different brands that are available in Costco. Two different popular coffee bean brands were bought and tested to determine which brand would have the highest caffeine content and their relative popularity among consumers. The extraction DMC method was conducted by using chemicals such as calcium carbonate, water, and DMC. The same amount of coffee beans were boiled with water until highly concentrated solutions were formed. Extraction funnel was utilized to wash out caffeine. Then, the recrystallization and vacuum filtration was utilized to obtain caffeine in solid form. The identity of the product along with the purity of the product was determined using melting temp, IR-spectroscopy, UV-vis spectrum, and TLC plating. The mass of caffeine produced from individual coffee brands were measured and compared. It was hypothesized that robusta coffee beans would yield more caffeine than arabica coffee beans. The expected results verify those claims as the data demonstrates that the amount of caffeine extracted from 10 grams of robusta coffee would be around .8021 grams, while the amount of caffeine extracted from 10 grams of arabica coffee would be around .4321 grams. The IR graph, UV-vis graph, and TLC plate were conducted to verify the identity of the product. The predicted IR graph, UV-vis graph, and TLC plate closely matched with the literature values, which indicates that the product produced is pure caffeine. One source of error that could skew the data could be the presence of impurities from the coffee beans that react in solution while we are trying to extract the caffeine. The broader impact of this review is that by understanding the caffeine content in different products, the medical and scientific field can further determine the difference in health effects between excess and optimal caffeine consumption to the human body. Additionally, scientists can research various medical usages of caffeine to help different patients with sleep disorders.

scholarly journals Changes of the chlorogenic acid, caffeine, gama-aminobutyric acid (GABA) and antioxdant activities during germination of coffee bean (Coffea arabica)

2018 ◽  
pp. 675 ◽  
Author(s):  
Yeokyeong Kim ◽  
Yeongyil Kim, and Deok-Young Jhon
Keyword(s):  
Chlorogenic Acid ◽  
Antioxidant Activities ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Functional Materials ◽  
Aminobutyric Acid ◽  
Assay Method ◽  
Total Phenolic ◽  
Coffee Bean ◽  
Coffee Beans

This study was carried out to identify changes in functional materials during the germination of coffee seeds. Chlorogenic acid (CGA), caffeine, and g-aminobutyric acid (GABA) was assayed by HPLC method while germinating coffee beans for 20 days. The contents of total phenolic compounds and total flavonoid compounds were measured by colorimetric method. Antioxidant activity was measured by both 2,2-diphenyl-2-pycryl hydrazyl (DPPH) radical scavenging activity assay and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) assay method. It was found that CGAs (5-caffeoylquinic acid (CQA), 4-CQA, 3-CQA), caffeine, and GABA increased and then decreased during germination. There were no significant changes in total phenolics, total flavonoids and antioxidant activities. Especially, all of the major isomers of CGA increased to the highest value at 10 days of germination, and GABA also increased significantly at the early stage of germination. This study showed that coffee beans changed into more functional food materials by germination and showed maximum functionality in appropriate germination conditions.

scholarly journals Optimization of Coffee Liqueur Manufacturing Process using Caffeine Content

2019 ◽  
Vol 9 (1) ◽  
pp. 430-433
Keyword(s):  
Alcoholic Beverage ◽  
The Other ◽  
Coffee Bean ◽  
Roasted Coffee ◽  
Caffeine Concentration ◽  
Caffeine Content ◽  
Other Hand ◽  
Coffee Beans ◽  
Roasted Coffee Beans

Coffee liqueur is an alcoholic beverage made by soaking coffee in spirit. Though coffee liqueur is one of the wellknown liqueur, few research was made so far. In this research, coffee liqueur was prepared by using different recipes involving liquors with different ethanol concentrations (20, 40, or 80%), and the soaking periods (14, 21, or 30 days) of the coffee liqueur ingredients (roasted coffee beans, coffee bean powder and other ingredients). Volatile and semi-volatile compounds are important for the organoleptic quality of roasted coffee beans. However, the amounts of these compounds in roasted coffee beans are more dependent on roasting conditions than those of non-volatiles. Furthermore, volatiles are more easily lost during storage. On the other hand, caffeine, a well-known compound of coffee is an intrinsic non-volatile chemical, and the caffeine is present in larger than other potential indicator components in coffee beans, ensuring the quantitative consistency of the coffee extracts in the liqueur. Caffeine was quantified from the prepared coffee liqueurs by HPLC after method validation. The average caffeine content of all 54 analyses (6 analyses per sample) was 2081.2 μg/mL. The highest caffeine concentration 2793 μg/mL was acquired in the sample prepared by 80% ethanol liquor. A soaking period of 14 days, was considered inadequate for sufficient caffeine extraction regardless of liquor alcohol content. Caffeine concentration peaked after soaking coffees and other ingredients for 21 days at 80% ethanol concentration. On the other hand, the most consistent caffeine concentrations in liqueurs were obtained by soaking for 21 days in 40% ethanol (RSD 7.8%), thus it could be the optimum coffee liqueur recipe. The consistency of coffee liqueur quality was assured using caffeine content.

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