Analysis of Oyster Plant (Tradescantia Spathacea) Extracts via Maceration, Soxhlet Extraction and Thin Layer Chromatograpy

2016 ◽  
Author(s):  
Maria Pina ◽  
Daniel Russo ◽  
Cristina Balistreri ◽  
Kelly O'Reilly ◽  
Luis Cendan ◽  
...  
Keyword(s):  
Thin Layer ◽  
Soxhlet Extraction

scholarly journals The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.)

2005 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
SOERYA DEWI MARLIANA ◽  
VENTY SURYANTI ◽  
SUYONO SUYONO
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Thin Layer Chromatography Analysis ◽  
Chromatography Analysis ◽  
Sechium Edule ◽  
Layer Chromatography

The phytochemical screenings and analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.) with Thin Layer Chromatography (TLC) has been carried out. Isolation was done by Soxhlet extraction for 6 hours with petroleum ether and the residue was extracted by maceration during 24 hours with ethanol.The isolated compounds in ethanol extract were identified by phytochemical screenings method and TLC. The result showed the presence of alkaloid, saponin, cardenolin/bufadienol and flavonoid.

scholarly journals Conversion of Monuron to Ethyl-N-p-chlorophenyl Carbamate by Soxhlet Extraction of Plant Tissue with Ethanol

1971 ◽  
Vol 54 (6) ◽  
pp. 1361-1365 ◽  
Author(s):  
Shaw S Lee ◽  
S C Fang
Keyword(s):  
Thin Layer ◽  
Paper Chromatography ◽  
Plant Tissue ◽  
Soxhlet Extraction ◽  
Plant Materials

Abstract Soxhlet extraction of plant materials with alcohol converted monuron residue to carbamate. Although this conversion will not affect the accuracy of monuron determination by the Bratton-Marshall reaction, it will undoubtedly affect the accuracy if the measurement is carried out by thin layer or paper chromatography. Diuron, fenuron, and chloroxuron, which contain 1,1-dimethyl substitution, were also converted to the corresponding carbamates, while siduron, neburon, linuron, monomethyl monuron, monomethyl diuron, and p-chlorophenyl urea were not. When monuron was refluxed with other alcohols such as methanol, isopropanol, and n-butanol, monuron was also converted to other products.

scholarly journals Isolation and NMR Characterization of Ursane-Type Triterpenoid from the Leaves of Peperomia pellucida

2021 ◽  
Vol 25 (3) ◽  
pp. 397-400
Author(s):  
J.C. Ibe–Diala ◽  
O.U. Igwe ◽  
C. Friday ◽  
U.C. Akwada
Keyword(s):  
Thin Layer ◽  
Extraction Method ◽  
Plant Material ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Isolation And Purification ◽  
Single Spot ◽  
Chromatographic Techniques ◽  
Nmr Characterization

Peperomia pellucida is a member of the Piperaceae family. Extraction of plant material was carried out by Soxhlet extraction method using hexane and ethylacetate as solvents respectively. The extract was concentrated using a rotary evaporator, followed by isolation and purification using column and thin layer chromatographic techniques. Fraction C20 showed a clearly defined single spot with Rf value of 0.51. Using 1HNMR, 13C-DEPT, COSY, HSQC and HMBC and by comparison with literature values, the structure of the compound was established as an Ursane-type triterpenoid. The use of P. pellucida in ethnomedicine for the treatment of various ailments could be attributed to the Ursane-type triterpenoid and other bioactive chemical compounds present in the plant. Keywords: Peperomia pellucida, extraction, isolation, chromatographic techniques, ursane-type triterpenoid

scholarly journals Phytochemical Analysis and Thin Layer Chromatography Profiling of Crude Extracts from Senna Occidentalis(Leaves)

2019 ◽  
Vol 2 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Lawal A.M ◽  
Abdullahi R ◽  
Ibrahim M.S ◽  
Kurfi M.Y ◽  
Khalid A ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Cardiac Glycosides ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Test Methods ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Layer Chromatography

Plants used for medicinal practices which were discovered since prehistoric stone ages are termed Medicinal plants, which are also referred to as medicinal herbs, since plants produces bioactive chemical compounds (phytochemicals), this research however, is concerned with the extraction using Soxhlet extraction technique, phytochemical screening using various test methods, which reveals the presence of anthraquinones (free anthraquinones and combined anthraquinones), carbohydrates, cardiac glycosides, glycosides, flavonoids, saponins, steroids/ terpenes, phenolic compounds and tannins, and absence of alkaloids for extracts of senna occidentalis and also, thin layer chromatography profiling which gives probable foundation for further structural elucidation amongst others. This research shows the presence of potent secondary metabolites present in the leaves of senna occidentalis (leaves).

Extraction Efficiencies for Pesticides in Crops: 14C-Benomyl Extraction from Mustard Greens and Radishes

1980 ◽  
Vol 63 (6) ◽  
pp. 1286-1290
Author(s):  
Willis B Wheeler ◽  
Neal P Thompson ◽  
Pio Andrade ◽  
Richard T Krause
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Extraction Efficiency ◽  
Soxhlet Extraction ◽  
Breakdown Product ◽  
Hydrolysis Of ◽  
Better Than

Abstract 14C-labeled benomyl [methyl l-(butylcarbamoyl)-2-benzimidazolecarbamate] suspended in a commercial benomyl formulation was sprayed on mustard greens and radishes. At 3 intervals after application, the crops were extracted with methanol, acetonitrile, or acetone. Crops were either blended and leached or repetitively blended followed by Soxhlet extraction. Essentially all olF the extractable radioactivity was removed by blending. The 14C was more difficult to extract from radishes than from mustard greens as time increased. Respective percentages of 14C extracted at 1, 7, and 14 days were 99, 98, and 97 jFrom mustard greens and 96, 88, and 79 from radishes. Methanol exhibited the highest extraction efficiency, and the blend-Soxhlet process was better than the blend-leach process. Thin layer ihromatography of the organic-soluble extracts indicated that the majority of 14C was recovered as methyl 2-benzimidazole carbamate (MBC), a breakdown product of benomyl. Acid hydrolysis of the extracted tissues released 30–50% of the residual 14C.

Analysis of Wool Lipids Using Thin-Layer Chromatography with Flame Ionization Detection

1988 ◽  
Vol 58 (10) ◽  
pp. 593-600 ◽  
Author(s):  
Ian H. Leaver ◽  
David M. Lewis ◽  
David J. Westmoreland
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Detection System ◽  
Soxhlet Extraction ◽  
Quantitative Detection ◽  
Flame Ionization Detection ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Layer Chromatography

This paper examines the usefulness of thin-layer chromatography (TLC), coupled with an automated quantitative detection system based on flame ionization detection (FID), for the qualitative and quantitative determination of lipids in wool. The latroscan TLC-FID system has been used to determine the composition of the solvent soluble material (internal lipids) isolated from wool after Soxhlet extraction with a chloroform/methanol azeotrope, and to investigate whether scouring treatments affect the composition of the internal lipids. Changes in the composition of wool grease that occur as a result of exposure to sunlight (behind glass) and during weathering in the fleece are also examined.

scholarly journals PHYTOCHEMICAL SCREENING AND DEVELOPMENT OF VARIOUS THIN LAYER CHROMATOGRAPHIC METHODS FOR DIFFERENT CEDRELA TOONA ROXB. FRUIT EXTRACTS

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Shah Kinjal H ◽  
Patel Piyush M.
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Active Ingredient ◽  
Chemical Constituents ◽  
Water Extract ◽  
Soxhlet Extraction ◽  
Powdered Material ◽  
Pharmacological Effects ◽  
Phytochemical Screening ◽  
Isolation Method

Air dried powdered material of the fruits of cedrela toona Roxb. was successively extracted with petroleum ether, hexane, acetone, methanol and water extract by soxhlet extraction and subjected to various qualitative chemical tests to determine presence of various phytoconstituents like alkaloids, glycosides, carbohydrates, phenolics and tannins, phtosterols, fixed oils and fats, proteins, amino acids, flavonoids, saponins etc. The various extracts of fruits of cedrela toona Roxb. were than subjected to thin layer chromatographic studies to identify the number and nature of the chemical constituents present. This study helps researchers for developmentof isolation method of active ingredient having vast pharmacological effects. Keywords: Cedrela toona, Extracts, Phytoconstituents, TLC

Applications of Photoelectron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 506-507
Author(s):  
William J. Baxter
Keyword(s):  
Electron Microscopy ◽  
Thermal Decomposition ◽  
Chemical Composition ◽  
Ultraviolet Radiation ◽  
Thin Layer ◽  
Edge Effects ◽  
Electron Optics ◽  
Surface Layers ◽  
Crystalline Orientation ◽  
Fluorescent Screen

In this form of electron microscopy, photoelectrons emitted from a metal by ultraviolet radiation are accelerated and imaged onto a fluorescent screen by conventional electron optics. image contrast is determined by spatial variations in the intensity of the photoemission. The dominant source of contrast is due to changes in the photoelectric work function, between surfaces of different crystalline orientation, or different chemical composition. Topographical variations produce a relatively weak contrast due to shadowing and edge effects.Since the photoelectrons originate from the surface layers (e.g. ∼5-10 nm for metals), photoelectron microscopy is surface sensitive. Thus to see the microstructure of a metal the thin layer (∼3 nm) of surface oxide must be removed, either by ion bombardment or by thermal decomposition in the vacuum of the microscope.

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