scholarly journals Isolation and NMR Characterization of Ursane-Type Triterpenoid from the Leaves of Peperomia pellucida

2021 ◽  
Vol 25 (3) ◽  
pp. 397-400
Author(s):  
J.C. Ibe–Diala ◽  
O.U. Igwe ◽  
C. Friday ◽  
U.C. Akwada
Keyword(s):  
Thin Layer ◽  
Extraction Method ◽  
Plant Material ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Isolation And Purification ◽  
Single Spot ◽  
Chromatographic Techniques ◽  
Nmr Characterization

Peperomia pellucida is a member of the Piperaceae family. Extraction of plant material was carried out by Soxhlet extraction method using hexane and ethylacetate as solvents respectively. The extract was concentrated using a rotary evaporator, followed by isolation and purification using column and thin layer chromatographic techniques. Fraction C20 showed a clearly defined single spot with Rf value of 0.51. Using 1HNMR, 13C-DEPT, COSY, HSQC and HMBC and by comparison with literature values, the structure of the compound was established as an Ursane-type triterpenoid. The use of P. pellucida in ethnomedicine for the treatment of various ailments could be attributed to the Ursane-type triterpenoid and other bioactive chemical compounds present in the plant. Keywords: Peperomia pellucida, extraction, isolation, chromatographic techniques, ursane-type triterpenoid

scholarly journals The phytochemical screenings and thin layer chromatography analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.)

2005 ◽  
Vol 3 (1) ◽  
pp. 26-31 ◽  
Author(s):  
SOERYA DEWI MARLIANA ◽  
VENTY SURYANTI ◽  
SUYONO SUYONO
Keyword(s):  
Thin Layer ◽  
Petroleum Ether ◽  
Thin Layer Chromatography ◽  
Soxhlet Extraction ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Thin Layer Chromatography Analysis ◽  
Chromatography Analysis ◽  
Sechium Edule ◽  
Layer Chromatography

The phytochemical screenings and analysis of chemical compounds in ethanol extract of labu siam fruit (Sechium edule Jacq. Swartz.) with Thin Layer Chromatography (TLC) has been carried out. Isolation was done by Soxhlet extraction for 6 hours with petroleum ether and the residue was extracted by maceration during 24 hours with ethanol.The isolated compounds in ethanol extract were identified by phytochemical screenings method and TLC. The result showed the presence of alkaloid, saponin, cardenolin/bufadienol and flavonoid.

Isolation and Identification of Paracetamol Metabolites

1976 ◽  
Vol 4 (4_suppl) ◽  
pp. 34-39 ◽  
Author(s):  
R S Andrews ◽  
C C Bond ◽  
J Burnett ◽  
A Saunders ◽  
K Watson
Keyword(s):  
Thin Layer ◽  
Urinary Metabolites ◽  
Two Dimensional ◽  
Isolation And Identification ◽  
Isolation And Purification ◽  
Chromatographic Techniques ◽  
Main Technique ◽  
Therapeutic Doses ◽  
Layer Chromatography ◽  
New Metabolites

A comparison has been made of the urinary metabolites of volunteers who had taken therapeutic doses of paracetamol with those of persons who had taken an overdose in an attempt to highlight the metabolic changes associated with massive doses. The main technique for examining urine samples was two-dimensional thin layer chromatography. Other chromatographic techniques were used for the isolation and purification of metabolites. The urinary metabolites after a therapeutic dose of paracetamol were identified as free paracetamol, paracetamol sulphate, 3-hydroxy-paracetamol-3-sulphate, 3-methoxy-paracetamol sulphate, paracetamol glucuronide, 3-methoxy-paracetamol glucuronide, paracetamol 3-cysteine conjugate and paracetamol 3-mercapturate. The same metabolites were also present in urine following overdosage but the proportions were quite different. There was particularly a big increase in the relative amounts of cysteine and mercapturic acid conjugates excreted. No new metabolites were found. The significance of these findings is briefly discussed in relation to the metabolism and toxicology of paracetamol.

scholarly journals Characterizing the Three Different Alginate Type of Sargassum siliquosum

2017 ◽  
Vol 22 (1) ◽  
pp. 7 ◽  
Author(s):  
Ervia Yudiati ◽  
Alim Isnansetyo
Keyword(s):  
Ir Spectroscopy ◽  
Thin Layer ◽  
Extraction Method ◽  
Calcium Alginate ◽  
Brown Seaweed ◽  
Guluronic Acid ◽  
Ft Ir ◽  
Layer Chromatography ◽  
Ft Ir Spectroscopy

This research was aimed to identify the brown seaweed, to characterize the acid, sodium and calcium alginate, and to examine the alginate yield. The identification was done phaenotypically. The extraction method was pretreated by ethanol depigmentation, followed by the extraction of Na2CO3/EDTA and CaCl2  and presipitated with absolute ethanol. The characterization of alginate was done by FT-IR spectroscopy and Thin Layer Chromatography by comparing the samples with standard alginate (Sigma, USA). The key of identification  showed that the species was Sargassum siliquosum. There are similarities in signal vibration and TLC spots among the samples and the standard. The TLC test was also showed that those alginates contain mannuronic and guluronic acid. The highest yield was produced by Sodium alginate (40,34% + 0,21), followed by Acid alginate (11,51% + 0,15) and Calcium alginate (4,8% + 0,09). Keywords: alginate, characterization, Sargassum siliquosum, yield

scholarly journals Phytochemical Analysis and Thin Layer Chromatography Profiling of Crude Extracts from Senna Occidentalis(Leaves)

2019 ◽  
Vol 2 (1) ◽  
pp. 12-21 ◽  
Author(s):  
Lawal A.M ◽  
Abdullahi R ◽  
Ibrahim M.S ◽  
Kurfi M.Y ◽  
Khalid A ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Cardiac Glycosides ◽  
Soxhlet Extraction ◽  
Chemical Compounds ◽  
Test Methods ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Layer Chromatography

Plants used for medicinal practices which were discovered since prehistoric stone ages are termed Medicinal plants, which are also referred to as medicinal herbs, since plants produces bioactive chemical compounds (phytochemicals), this research however, is concerned with the extraction using Soxhlet extraction technique, phytochemical screening using various test methods, which reveals the presence of anthraquinones (free anthraquinones and combined anthraquinones), carbohydrates, cardiac glycosides, glycosides, flavonoids, saponins, steroids/ terpenes, phenolic compounds and tannins, and absence of alkaloids for extracts of senna occidentalis and also, thin layer chromatography profiling which gives probable foundation for further structural elucidation amongst others. This research shows the presence of potent secondary metabolites present in the leaves of senna occidentalis (leaves).

SUGGESTIVE PLACEMENT OF Quassia borneensis Noot. (Simaroubaceae) AS INDICATED BY ISOLATION OF ITS QUASSINOIDS

2015 ◽  
Vol 77 (2) ◽  
Author(s):  
Julenah Ag Nuddin ◽  
Aishah Adam ◽  
Ahmad Sazali Hamzah
Keyword(s):  
Biological Activity ◽  
Secondary Metabolites ◽  
Methanol Extract ◽  
Biological Activities ◽  
Data Gathering ◽  
Chemical Compounds ◽  
Structural Elucidation ◽  
Aqueous Extracts ◽  
Isolation And Purification ◽  
Chromatographic Techniques

Quassinoids have been reported to possess numerous biological activities such as anticancer, antimalarial and phytotoxicity. They are degraded triterpenoids which give bitterness characteristic of Simaroubaceae and classified according to their basic skeletons; C18, C19, C20, C22, C25 which correspond to their particular genus Quassia, Brucea, Soulamea, Eurycoma, Picrolemnia and others. Quassia borneensis Noot. (Simaroubaceae) (Qb) have not been properly placed in Quassia s.l. thus created confusion. Therefore, a study on Qb for its chemical compounds and biological activity are appropriate for data gathering. There is positive possibility for new quassinoids with potent biological activity to be discovered, in addition to confirmation of placement in Simaroubaceae. The study was accomplished by extracting the secondary metabolites from Qb by soxhlet in methanol. The methanol extract was partitioned to acquire n-hexane, chloroform and aqueous extracts. These extracts underwent isolation and purification through chromatographic techniques before subjected to spectroscopic instruments for structural elucidation. Preliminary findings identified five quassinoids and one canthin-6-one as glaucarubolone (1), chaparrinone (2), holacanthone (3), glaucarubinone (4), ailanthinone (5) and canthin-6-one (6) in reference to past reports. The isolation confirms Qb placement in Simaroubaceae, however, not in Quassia section as indicated by isolated quassinoids. Hence, further study would confirm its proper section in Quassia.

scholarly journals NMR characterization and free radical scavenging activity of pheophytin ‘A’ from the leaves of Dissotis rotundifolia

2021 ◽  
Vol 35 (1) ◽  
pp. 207-215
Author(s):  
C. Friday ◽  
O. UchennaIgwe ◽  
U. C. Akwada
Keyword(s):  
Free Radical ◽  
Thin Layer ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Pheophytin A ◽  
Chromatographic Techniques ◽  
Nmr Characterization

Dissotis rotundifolia is used traditionally in various parts of Africa for the treatment of various ailments. The air dried and pulverized leaves of Dissotis rotundifolia was extracted with distilled n-hexane and ethylacetate, using Soxhlet apparatus. Isolation and purification were carried out using column and thin layer chromatographic techniques. Fraction B14 gave a single spot on thin layer chromatography with a retention factor (Rf) value of 0.57. Characterization of B14 was doneusing 1H-NMR, 13C-DEPT, COSY, HSQC, HMBC and by direct comparison with literature values and pheophytin A was proposed as the structure of the compound. Pheophytin A exhibited a free radical scavenging activity of 10.10±0.05 and 19.51±0.02% at minimum and maximum concentrations of 1.00 and 4.00 mg/mL, respectively. The acclaimed ethnomedicinal uses of D. rotundifolia by the African natives could be linked to the presence of pheophytin A and other phytoconstituents in the plant. This is the first report of the isolation of pheophytin A from D. rotundifolia.                     KEY WORDS: Dissotis rotundifolia, Pheophytin A, Chromatographic techniques, Free radicals, NMR   Bull. Chem. Soc. Ethiop. 2021, 35(1), 207-215. DOI: https://dx.doi.org/10.4314/bcse.v35i1.18      

NMR characterization of complex natural products: Assigning novel, proton-deficient alkaloid scaffolds

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
KR Gustafson ◽  
STS Chan ◽  
D Milanowski
Keyword(s):  
Natural Products ◽  
Nmr Characterization
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