Extraction Efficiencies for Pesticides in Crops: 14C-Benomyl Extraction from Mustard Greens and Radishes

1980 ◽  
Vol 63 (6) ◽  
pp. 1286-1290
Author(s):  
Willis B Wheeler ◽  
Neal P Thompson ◽  
Pio Andrade ◽  
Richard T Krause
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Extraction Efficiency ◽  
Soxhlet Extraction ◽  
Breakdown Product ◽  
Hydrolysis Of ◽  
Better Than

Abstract 14C-labeled benomyl [methyl l-(butylcarbamoyl)-2-benzimidazolecarbamate] suspended in a commercial benomyl formulation was sprayed on mustard greens and radishes. At 3 intervals after application, the crops were extracted with methanol, acetonitrile, or acetone. Crops were either blended and leached or repetitively blended followed by Soxhlet extraction. Essentially all olF the extractable radioactivity was removed by blending. The 14C was more difficult to extract from radishes than from mustard greens as time increased. Respective percentages of 14C extracted at 1, 7, and 14 days were 99, 98, and 97 jFrom mustard greens and 96, 88, and 79 from radishes. Methanol exhibited the highest extraction efficiency, and the blend-Soxhlet process was better than the blend-leach process. Thin layer ihromatography of the organic-soluble extracts indicated that the majority of 14C was recovered as methyl 2-benzimidazole carbamate (MBC), a breakdown product of benomyl. Acid hydrolysis of the extracted tissues released 30–50% of the residual 14C.

scholarly journals ISOLATION AND ANALYSIS OF STEROIDAL SAPONINS FROM POLYGONATUM ODORATUM (MILL.) DRUCE

2021 ◽  
Vol 55 ◽  
pp. 135-140
Author(s):  
Cristina MOGOSAN ◽  
Ilioara ONIGA ◽  
Mircea TAMAS
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Biological Properties ◽  
Steroidal Saponins ◽  
Physico Chemical ◽  
Layer Chromatography ◽  
Hydrolysis Of

We isolated the steroidal saponins from the rhizomes of Polygonatum odoratum (Mill.) Druce with an efficiency of 4.50% which represents 7 fractions identified by thin-layer chromatography (TLC), of which 3 were furostanics and 4 spirostanics. After the acid hydrolysis of the saponins, one aglycone (sapogenine) was identified by TLC. Further, we have determined the physico-chemical and the biological properties of the isolated saponins.

scholarly journals HPTLC determination of diosgenin in fenugreek seeds

2018 ◽  
Vol 68 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Barbara Król-Kogus ◽  
Khenifi Mohammed Lamine ◽  
Piotr Migas ◽  
Messaoud Boudjeniba ◽  
Mirosława Krauze-Baranowska
Keyword(s):  
Ethyl Acetate ◽  
Acid Hydrolysis ◽  
Methanolic Extract ◽  
Soxhlet Extraction ◽  
African Origin ◽  
Fenugreek Seeds ◽  
Hydrolysis Products ◽  
Hydrolysis Of

Abstract A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent. The method was preliminarily validated and the determined amounts of diosgenin in fenugreek seeds of Polish and African origin were found to be similar and ranged from 0.12-0.18 %.

scholarly journals Acid Hydrolysis of Phenylthiohydantoins of Amino Acids

1971 ◽  
Vol 24 (4) ◽  
pp. 1247 ◽  
Author(s):  
AS Inglis ◽  
PW Nicholls ◽  
CM Roxburgh
Keyword(s):  
Amino Acids ◽  
Hydrochloric Acid ◽  
Thin Layer ◽  
Acid Hydrolysis ◽  
Free Amino ◽  
Hydriodic Acid ◽  
Ultraviolet Spectroscopy ◽  
Quantitative Identification ◽  
Layer Chromatography ◽  
Hydrolysis Of

The phenylthiohydantoins (PTHs) derived from amino acids were hydrolysed in boiling hydriodic acid for 24 hr. Good yields of free amino acids were obtained for all PTH derivatives except methionine. In contrast to hydrolysis with hydrochloric acid, hydrolysis with hydriodic acid converts PTH-threonine, PTH-serine, and PTH-tryptophan respectively to oc-amino-n-butyric acid, alanine, and a mixture (approx. 2: 1) of glycine and alanine. This procedure provides a useful adjunct to thin-layer chromatography and ultraviolet spectroscopy for quantitative identification of the PTH derivative.

scholarly journals EXTRACTION AND ACID HYDROLYSES OF FRESH JERUSALEM ARTICHOKE INULIN FOR FRUCTOOLIGOSACCHARIDE PRODUCTION

2019 ◽  
Vol 50 (6) ◽  
Author(s):  
Alabadi & Abood
Keyword(s):  
Qualitative Analysis ◽  
Acid Hydrolysis ◽  
Extraction Efficiency ◽  
Jerusalem Artichoke ◽  
Degree Of Polymerization ◽  
Hot Water ◽  
Hot Water Extraction ◽  
Ph Values ◽  
Rp Hplc ◽  
Hydrolysis Of

The objective of this study was to produce fructooligosaccharide (FOS) from fresh jerusalem artichoke (JAT) inulin which was extracted by conventional method (hot water extraction) at two different temperature (80˚C & 90˚C\ 90 min). JAT slices were mixed with distilled water at (1 : 5) (w : v) ratio, the obtained extract purified and concentrated to 32˚Brix, then mixed with acetone (1 : 3) (v : v), kept at 4˚C for 24 h., centrifuged at 10000g/ 15 min., and the precipitate dried at 55˚C. Acid hydrolysis of the dried inulin was carried out at three different pH values being (1.5, 2.5 & 3.0) at 85˚C using citric acid (10 %), aliquot of inulin hydrolysate were taken after (5, 10, 15, 20, 25 & 30) min. for qualitative analysis using TLC and RP-HPLC. The results revealed that there were no significant differences in the yield of extracted inulin (33.86, 33.67 %) and extraction efficiency (80.62, 80.17 %) between the extraction at 80˚C and 90˚C\ 90 min. respectively. The optimum condition for acid hydrolysis of the obtained inulin was at pH 2.5 after 15 min at 85˚C. The qualitative analysis for hydrolysate using TLC and RP-HPLC showed that the degree of polymerization (DP) of inulin ranged from (2 - 35) unit and for FOS ranged from (2 – 9) units.   

DETERMINATION OF MICRO-AMOUNTS OF 5β-PREGNAN-3α-OL-20-ONE IN URINE USING INFRARED-SPECTROMETRY

1962 ◽  
Vol 41 (2) ◽  
pp. 234-246 ◽  
Author(s):  
H. J. van der Molen
Keyword(s):  
Infrared Spectrum ◽  
Quantitative Determination ◽  
Acid Hydrolysis ◽  
Chromatographic Separation ◽  
Pure Form ◽  
Infrared Spectrometry ◽  
Hydrolysis Of

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.

Optimization of technological modes of cold acid hydrolysis of potato starch

2020 ◽  
Vol 26 (3) ◽  
pp. 222-233
Author(s):  
M. Alekseenko ◽  
V. Litvyak ◽  
A. Sysa ◽  
E. Hrabovska ◽  
O. Galenko
Keyword(s):  
Acid Hydrolysis ◽  
Potato Starch ◽  
Hydrolysis Of

FLOWTHROUGH ACID HYDROLYSIS OF RAPESEED STALKS

2012 ◽  
Vol 11 (12) ◽  
pp. 2313-2318
Author(s):  
Theodor Malutan ◽  
Adina Elena Panzariu
Keyword(s):  
Acid Hydrolysis ◽  
Hydrolysis Of

Analysis of Oyster Plant (Tradescantia Spathacea) Extracts via Maceration, Soxhlet Extraction and Thin Layer Chromatograpy

2016 ◽  
Author(s):  
Maria Pina ◽  
Daniel Russo ◽  
Cristina Balistreri ◽  
Kelly O'Reilly ◽  
Luis Cendan ◽  
...  
Keyword(s):  
Thin Layer ◽  
Soxhlet Extraction
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