scholarly journals Identification and Growth Inhibitory Activity of the Chemical Constituents from Imperata Cylindrica Aerial Part Ethyl Acetate Extract

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1807 ◽  
Author(s):  
Yan Wang ◽  
James Shen ◽  
Yuk Chan ◽  
Wing Ho
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Aerial Part ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Imperata Cylindrica ◽  
Growth Inhibitory ◽  
Ht 29

Imperata cylindrica (L.) Raeusch. (IMP) aerial part ethyl acetate extract has anti-proliferative, pro-apoptotic, and pro-oxidative effects towards colorectal cancer in vitro. The chemical constituents of IMP aerial part ethyl acetate extract were isolated using high-performance liquid chromatography (HPLC) and identified with tandem mass spectrometry (ESI-MS/MS) in combination with ultraviolet-visible spectrophotometry and 400 MHz NMR. The growth inhibitory effects of each identified component on BT-549 (breast) and HT-29 (colon) cancer cell lines were evaluated after 48/72 h treatment by MTT assay. Four isolated compounds were identified as trans-p-Coumaric acid (1); 2-Methoxyestrone (2); 11, 16-Dihydroxypregn-4-ene-3, 20-dione (3); and Tricin (4). Compounds (2), (3), and (4) exhibited considerable growth inhibitory activities against BT-549 and HT-29 cancer cell lines. Compounds (2), (3), and (4) are potential candidates for novel anti-cancer agents against breast and colorectal cancers.

Isolation of Cardamonin and Pinostrobin Chalcone from the Rhizomes of Boesenbergia rotunda (L.) Mansf. and their Cytotoxic Effects on H-29 and MDA-MB-231 Cancer Cell Lines

2019 ◽  
Vol 9 (4) ◽  
pp. 341-348 ◽  
Author(s):  
Ibrahim Awad Mohammed ◽  
Muhammad Nadeem Akhtar ◽  
Foo Jhi Biau ◽  
Yin Sim Tor ◽  
Seema Zareen ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Colon Cancer Cell Lines ◽  
Ht 29

<P>Background: Breast cancer and human colon cancer are the most common types of cancer in females and males, respectively. Breast cancer is the most common type of cancer after lung and colon cancers. Natural products are an important source for drug discovery. Boesenbergia rotunda (L.) Mansf. is commonly known as finger root, belonging to the Zingiberaceae family. </P><P> Objective: The aim of this study to isolate some natural compounds from the rhizomes of B. rotunda (L.) Mansf., and to investigate their cytotoxicity against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines. </P><P> Methods: The dried rhizomes of B. rotunda were extracted with methanol. The methanolic extract was further used for solvent-solvent extraction. Bioassay-guided extraction and isolation of the rhizomes of the B. rotunda exhibited cytotoxic properties of hexane and dichloromethane fractions. </P><P> Results: Six major chemical constituents, pinostrobin (1), pinostrobin chalcone (2), cardamonin (3), 4,5-dihydrokawain (4), pinocembrin (5), and alpinetin (6) were isolated from the rhizomes of the B. rotunda. All the chemical constituents were screened against the human triple-negative breast cancer cell (MDA-MB-231) and HT-29 colon cancer cell lines. The compound cardamonin (3) (IC50 = 5.62&#177;0.61 and 4.44&#177;0.66 &#181;g/mL) and pinostrobin chalcone (2), (IC50 = 20.42&#177;2.23 and 22.51&#177;0.42 μg/mL) were found to be potent natural cytotoxic compounds against MDA-MB-231 and HT-29 colon cancer cell lines, respectively. </P><P> Conclusion: Cardamonin (3) and pinostrobin chalcone (2) were found to be the most potential natural compounds against breast cancer cell line MDA-MB-231 and colon cancer HT-29 cell line.</P>

Anastatica hierochuntica Extracts: Promising Safe and Selective Anticancer Agents

2020 ◽  
Vol 10 ◽  
Author(s):  
Zaynab Al-Eisawi ◽  
Salim M. Abderrahman ◽  
Yasmin M. S. Abdelrahim ◽  
Reem Al-Abbassi ◽  
Yasser K. Bustanji
Keyword(s):  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Anticancer Agents ◽  
Bone Marrow Cells ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Dermal Fibroblasts ◽  
Very High

Background: Medicinal plants have been used for many centuries for their significant phytochemical reservoir, forming a foundation for many medicinal drugs. Conventional cancer therapy possesses a number of undesired problems including poor selectivity, severe side effects and resistance of cancer. Objective: There is a need for new anticancer drugs possibly from natural sources such as medicinal plants. A. Hierochuntica L. is rich with phytochemicals and is widely used in traditional medicine. Method: The antiproliferative activities of A. hierochuntica whole plant extracts (ethanol, methanol, ethyl acetate, chloroform and water) were determined against a panel of cancer cells and normal primary dermal fibroblasts. The genotoxic effect of A. hierochuntica ethyl acetate on mice bone marrow cells was also evaluated. Genotoxic parameters included Chromosomal aberration, Mitodepression, Micronuclei formation and sister chromatid exchange. Results: A. hierochuntica exhibited antiproliferative activity against leukemia (K-562) and melanoma (A-375) cells. This is the first study to demonstrate the effect of A. hierochuntica ethyl acetate extract on cancer cell lines, found to be the most cytotoxic of all extracts. Furthermore, the chloroform extract showed notable anti-proliferative effects against most cancer cell lines tested. The extracts proved to be highly safe on human normal skin fibroblasts. Furthermore, A. hierochuntica ethyl acetate extract was also found to have limited genotoxic effects, with these changes seen at very high doses only. Conclusion: The results indicate that A. hierochuntica extracts have significant selective anticancer activity and that genotoxicity is only observed at very high concentrations.

scholarly journals Isolation and Evaluation of Bioactive Compounds from Rheum emodi and their Anti-Inflammatory and Anticancer Properties

2021 ◽  
Vol 25 (06) ◽  
pp. 1161-1172
Author(s):  
Sang Koo Park
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Flavonoid Compound ◽  
Dependent Manner ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Mcf 7

Rheum emodi Wall. ex Meissn is a popular medicinal herb having wide application in traditional medicine for treating of several diseases. The present study was aimed to identify and isolate phytochemicals present in ethyl acetate extract fraction of R. emodi and to evaluate the anticancer and anti-inflammatory activities of water/organic solvent fractions and isolated compounds of R. emodi rhizome extracts. Based on the structure, flavonoid compound i.e., Myricitrin (sym. Myricetin 3- rhamnoside), myricetin 3-galloylrhamnoside and myricetin were identified to be present in ethyl acetate extract. The molecular weight of compound 1 cannot be identified; while compound 5 remained unknown as there was not enough evidence to propose its structure. The isolated compounds and different solvent fractions were tested for their anticancer and antiinflammatory activities. Among Myricetins derivatives, particularly unknown compounds significantly induced the apoptosis and restrained the proliferation of cancer cell lines (A549 and MCF-7) and inhibited the LPS induced NO production (proinflammatory mediator), IL-6 activity, IL-1β and TNF-α (cytokines) activity in RAW 264.7 macrophages in a dose dependent manner and was effective even at lower concentration (50 µg/mL). Similarly, the higher concentration of aqueous and solvent fractions exhibited strong cytotoxic and anti-inflammatory activities. However, water and dichloromethane fractions were most effective in inhibiting the anticancer activities in A549 and MCF-7 cancer cell lines, respectively. At lower concentration (50 and 100 µg/mL), highest inhibition activity for NO, IL-6 and IL-1β was noted with ethyl acetate fractions and n-Hexane fractions; while water and n-Butanol (fractions) strongly inhibited the TNF-α activity at lower (100 µg/mL) and high (200 µg/mL) concentration, respectively. In conclusion, the isolated compounds from R. emodi rhizome extracts and its rhizome solvent fractions exhibit strong anti-cancer and anti-inflammatory activities and can be used to develop chemotherapeutics and anti-inflammation drugs. © 2021 Friends Science Publishers

scholarly journals Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4009
Author(s):  
Ahmed S. Abu Zaid ◽  
Ahmed E. Aleissawy ◽  
Ibrahim S. Yahia ◽  
Mahmoud A. Yassien ◽  
Nadia A. Hassouna ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Streptomyces Griseus ◽  
Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Chemical Structures ◽  
Bioassay Guided Fractionation

Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various cancer cell lines. Methods: Production of cytotoxic metabolites from S. griseus isolate KJ623766 was carried out in a 14L laboratory fermenter under specified optimum conditions. Using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined. Bioassay-guided fractionation of the ethyl acetate extract using different chromatographic techniques was used for cytotoxic metabolite purification. Chemical structures of the purified metabolites were identified using mass, 1D, and 2D NMR spectroscopic analysis. Results: Bioassay-guided fractionation of the ethyl acetate extract led to the purification of two cytotoxic metabolites, R1 and R2, of reproducible amounts of 5 and 1.5 mg/L, respectively. The structures of R1 and R2 metabolites were identified as β- and γ-rhodomycinone with CD50 of 6.3, 9.45, 64.8 and 9.11, 9.35, 67.3 µg/mL against Caco2, Hela and Vero cell lines, respectively. Values were comparable to those of the positive control doxorubicin. Conclusions: This is the first report about the production of β- and γ-rhodomycinone, two important scaffolds for synthesis of anticancer drugs, from S. griseus.

Evaluation of Anticancer Property of Claviceps Purpurea Secondary Metabolites on Different Cancer Cell Lines

2020 ◽  
Author(s):  
Lokesh ST ◽  
Thippeswamy Basaiah ◽  
Sowmya HV ◽  
Vijaya Kumar ML
Keyword(s):  
Cell Cycle ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Dna Fragmentation ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Nuclear Staining ◽  
Claviceps Purpurea

Abstract The present study deals with the secondary metabolites of Claviceps purpurea extract, which has been confirmed as an empirically potential cure for cancer. The purpose of this research was to prove scientifically the anticancer potential of ethyl acetate extract as its effects on the cell cycle, membrane apoptosis, DNA fragmentation and nuclear staining of lung cancer (A549) cell lines. The MTT method was used to measure cytotoxic effects, ethyl acetate extract of Claviceps purpurea showed IC50 value of 56.38 µg/ml compare to other cancer cell lines like breast cancer (MCF-7) cell lines (90.04 µg/ml) and cervical cancer (HeLa) cell lines (84.97 µg/ml) and normal cell lines fibroblast (3T3L1) does not show IC50 values due to lesser percentage of inhibition. The effect of inhibition of cell cycle and induction of cell apoptosis was measured by the flow cytometry method. DNA fragmentation studied by the gel electrophoresis method. The results showed, the ethyl acetate extract of Claviceps purpurea gives an induction of apoptosis effect on A549 cancer cell through inhibition of cell cycle in the G0-G1, Synthesis, and G2/M phases. In necrotic cell regions apoptosis was found. In DNA fragmentation 80 μg/ml concentration of extract showed significant results against A549 cell lines. In the nuclear staining study, membrane blebbing will be found. It indicates the extract was very active against cancer cell lines and it occurs in late apoptotic phase. In this study of all parameters, Colchicine was used as standard for cell cycle studies, for membrane apoptosis studies vinblastine and DNA fragmentation studies hydrogen peroxide was used.

scholarly journals Study on the chemical constituents of Dacrydium elatum and their cytotoxic activity

2019 ◽  
Vol 74 (2) ◽  
pp. 197-201
Author(s):  
Tran Thi Phuong Thao ◽  
Nguyen Thi Lieu ◽  
Pham Thi Ninh ◽  
Tran Van Chien ◽  
Dinh Thi Phong ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Etoac Extract ◽  
Extensive Analysis ◽  
Medium Activity ◽  
Ft Ir ◽  
Mcf 7

AbstractFrom an ethyl acetate extract of twigs and barks of Dacrydium elatum a new diterpenoid named dacrydianone (1), together with lambertic acid (2), three ecdysteroids: 20-hydroxyecdysone (3), ajugasterone C (4), ponasterone A (5), and daucosterol (6), has been isolated. Their structures were elucidated by an extensive analysis of the UV/Vis, FT-IR, MS and NMR spectra as well as comparison with those in published literature. The EtOAc extract and the isolated compounds 1–5 were evaluated for their cytotoxicity on four cancer cell lines: breast cancer (MCF-7), lung cancer (Lu-1), liver cancer (HepG2) and human oral cancer (KB). The EtOAc extract and compound 2 showed a medium activity on four tested cancer cell lines, while compounds 3 and 5 exhibited week activity on these cell lines. Compounds 1 and 4 did not show activity on the tested cell lines.

scholarly journals Acanthomine A, a new Pyrimidine-β-Carboline Alkaloid from the Sponge Acanthostrongylophora Ingens

2008 ◽  
Vol 3 (2) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Sabrin R. M. Ibrahim ◽  
RuAngelie Ebel ◽  
Rainer Ebel ◽  
Peter Proksch
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Brine Shrimp ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Chemical Investigation

Chemical investigation of the ethyl acetate extract of the sponge Acanthostrongylophora ingens afford one new pyrimidine-β-carboline alkaloid named acanthomine A (2), together with two known compounds annomontine (1) and 1,2,3,4-tetrahydronorharman-1-one (3). Their structures were unambiguously established on the basis of NMR spectroscopy (1H, 13C, 1H-1H COSY, HMQC and HMBC) and mass spectrometry. The isolated compounds were tested for cytotoxic activity using brine shrimp bioassay and different cancer cell lines.

Study on Chemical Constituents and Cytotoxic Activities of Salacia chinensis Growing in Vietnam

2010 ◽  
Vol 65 (10) ◽  
pp. 1284-1288 ◽  
Author(s):  
Tran Thi Minh ◽  
Nguyen Thi Hoang Anh ◽  
Vu Dao Thang ◽  
Tran Van Sung
Keyword(s):  
Spectral Analysis ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Cytotoxic Activities ◽  
Good Activity ◽  
Hep G2 ◽  
Mcf 7

Two new triterpenoids, named 7α,21α-dihydroxyfriedelane-3-one (1) and 7α,29-dihydroxyfriedelane- 3-one (2) have been isolated from the ethyl acetate extract of the stems of Salacia chinensis besides the known triterpenoid 21α,30-dihydroxyfriedelane-3-one (3). The structures of the isolated compounds were elucidated on the basis of spectral analysis. Eight triterpenoids from this plant have been tested against the four cancer cell lines Hep-G2, LU, KB, and MCF-7. The new compound 1 showed good activity against all four tested cell lines.

Sign in / Sign up

Export Citation Format

Share Document