Cytotoxic effects of ethyl acetate extract of Sambucus ebulus compared with Etoposide on normal and cancer cell lines

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
SS Saeedi Saravi ◽  
M Shokrzadeh ◽  
M Mirzayi
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Cytotoxic Effects ◽  
Sambucus Ebulus

Anastatica hierochuntica Extracts: Promising Safe and Selective Anticancer Agents

2020 ◽  
Vol 10 ◽  
Author(s):  
Zaynab Al-Eisawi ◽  
Salim M. Abderrahman ◽  
Yasmin M. S. Abdelrahim ◽  
Reem Al-Abbassi ◽  
Yasser K. Bustanji
Keyword(s):  
Medicinal Plants ◽  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Anticancer Agents ◽  
Bone Marrow Cells ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Dermal Fibroblasts ◽  
Very High

Background: Medicinal plants have been used for many centuries for their significant phytochemical reservoir, forming a foundation for many medicinal drugs. Conventional cancer therapy possesses a number of undesired problems including poor selectivity, severe side effects and resistance of cancer. Objective: There is a need for new anticancer drugs possibly from natural sources such as medicinal plants. A. Hierochuntica L. is rich with phytochemicals and is widely used in traditional medicine. Method: The antiproliferative activities of A. hierochuntica whole plant extracts (ethanol, methanol, ethyl acetate, chloroform and water) were determined against a panel of cancer cells and normal primary dermal fibroblasts. The genotoxic effect of A. hierochuntica ethyl acetate on mice bone marrow cells was also evaluated. Genotoxic parameters included Chromosomal aberration, Mitodepression, Micronuclei formation and sister chromatid exchange. Results: A. hierochuntica exhibited antiproliferative activity against leukemia (K-562) and melanoma (A-375) cells. This is the first study to demonstrate the effect of A. hierochuntica ethyl acetate extract on cancer cell lines, found to be the most cytotoxic of all extracts. Furthermore, the chloroform extract showed notable anti-proliferative effects against most cancer cell lines tested. The extracts proved to be highly safe on human normal skin fibroblasts. Furthermore, A. hierochuntica ethyl acetate extract was also found to have limited genotoxic effects, with these changes seen at very high doses only. Conclusion: The results indicate that A. hierochuntica extracts have significant selective anticancer activity and that genotoxicity is only observed at very high concentrations.

scholarly journals Isolation and Evaluation of Bioactive Compounds from Rheum emodi and their Anti-Inflammatory and Anticancer Properties

2021 ◽  
Vol 25 (06) ◽  
pp. 1161-1172
Author(s):  
Sang Koo Park
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Flavonoid Compound ◽  
Dependent Manner ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Mcf 7

Rheum emodi Wall. ex Meissn is a popular medicinal herb having wide application in traditional medicine for treating of several diseases. The present study was aimed to identify and isolate phytochemicals present in ethyl acetate extract fraction of R. emodi and to evaluate the anticancer and anti-inflammatory activities of water/organic solvent fractions and isolated compounds of R. emodi rhizome extracts. Based on the structure, flavonoid compound i.e., Myricitrin (sym. Myricetin 3- rhamnoside), myricetin 3-galloylrhamnoside and myricetin were identified to be present in ethyl acetate extract. The molecular weight of compound 1 cannot be identified; while compound 5 remained unknown as there was not enough evidence to propose its structure. The isolated compounds and different solvent fractions were tested for their anticancer and antiinflammatory activities. Among Myricetins derivatives, particularly unknown compounds significantly induced the apoptosis and restrained the proliferation of cancer cell lines (A549 and MCF-7) and inhibited the LPS induced NO production (proinflammatory mediator), IL-6 activity, IL-1β and TNF-α (cytokines) activity in RAW 264.7 macrophages in a dose dependent manner and was effective even at lower concentration (50 µg/mL). Similarly, the higher concentration of aqueous and solvent fractions exhibited strong cytotoxic and anti-inflammatory activities. However, water and dichloromethane fractions were most effective in inhibiting the anticancer activities in A549 and MCF-7 cancer cell lines, respectively. At lower concentration (50 and 100 µg/mL), highest inhibition activity for NO, IL-6 and IL-1β was noted with ethyl acetate fractions and n-Hexane fractions; while water and n-Butanol (fractions) strongly inhibited the TNF-α activity at lower (100 µg/mL) and high (200 µg/mL) concentration, respectively. In conclusion, the isolated compounds from R. emodi rhizome extracts and its rhizome solvent fractions exhibit strong anti-cancer and anti-inflammatory activities and can be used to develop chemotherapeutics and anti-inflammation drugs. © 2021 Friends Science Publishers

scholarly journals Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4009
Author(s):  
Ahmed S. Abu Zaid ◽  
Ahmed E. Aleissawy ◽  
Ibrahim S. Yahia ◽  
Mahmoud A. Yassien ◽  
Nadia A. Hassouna ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Streptomyces Griseus ◽  
Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Chemical Structures ◽  
Bioassay Guided Fractionation

Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various cancer cell lines. Methods: Production of cytotoxic metabolites from S. griseus isolate KJ623766 was carried out in a 14L laboratory fermenter under specified optimum conditions. Using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined. Bioassay-guided fractionation of the ethyl acetate extract using different chromatographic techniques was used for cytotoxic metabolite purification. Chemical structures of the purified metabolites were identified using mass, 1D, and 2D NMR spectroscopic analysis. Results: Bioassay-guided fractionation of the ethyl acetate extract led to the purification of two cytotoxic metabolites, R1 and R2, of reproducible amounts of 5 and 1.5 mg/L, respectively. The structures of R1 and R2 metabolites were identified as β- and γ-rhodomycinone with CD50 of 6.3, 9.45, 64.8 and 9.11, 9.35, 67.3 µg/mL against Caco2, Hela and Vero cell lines, respectively. Values were comparable to those of the positive control doxorubicin. Conclusions: This is the first report about the production of β- and γ-rhodomycinone, two important scaffolds for synthesis of anticancer drugs, from S. griseus.

Evaluation of Anticancer Property of Claviceps Purpurea Secondary Metabolites on Different Cancer Cell Lines

2020 ◽  
Author(s):  
Lokesh ST ◽  
Thippeswamy Basaiah ◽  
Sowmya HV ◽  
Vijaya Kumar ML
Keyword(s):  
Cell Cycle ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Dna Fragmentation ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Nuclear Staining ◽  
Claviceps Purpurea

Abstract The present study deals with the secondary metabolites of Claviceps purpurea extract, which has been confirmed as an empirically potential cure for cancer. The purpose of this research was to prove scientifically the anticancer potential of ethyl acetate extract as its effects on the cell cycle, membrane apoptosis, DNA fragmentation and nuclear staining of lung cancer (A549) cell lines. The MTT method was used to measure cytotoxic effects, ethyl acetate extract of Claviceps purpurea showed IC50 value of 56.38 µg/ml compare to other cancer cell lines like breast cancer (MCF-7) cell lines (90.04 µg/ml) and cervical cancer (HeLa) cell lines (84.97 µg/ml) and normal cell lines fibroblast (3T3L1) does not show IC50 values due to lesser percentage of inhibition. The effect of inhibition of cell cycle and induction of cell apoptosis was measured by the flow cytometry method. DNA fragmentation studied by the gel electrophoresis method. The results showed, the ethyl acetate extract of Claviceps purpurea gives an induction of apoptosis effect on A549 cancer cell through inhibition of cell cycle in the G0-G1, Synthesis, and G2/M phases. In necrotic cell regions apoptosis was found. In DNA fragmentation 80 μg/ml concentration of extract showed significant results against A549 cell lines. In the nuclear staining study, membrane blebbing will be found. It indicates the extract was very active against cancer cell lines and it occurs in late apoptotic phase. In this study of all parameters, Colchicine was used as standard for cell cycle studies, for membrane apoptosis studies vinblastine and DNA fragmentation studies hydrogen peroxide was used.

scholarly journals Identification and Growth Inhibitory Activity of the Chemical Constituents from Imperata Cylindrica Aerial Part Ethyl Acetate Extract

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1807 ◽  
Author(s):  
Yan Wang ◽  
James Shen ◽  
Yuk Chan ◽  
Wing Ho
Keyword(s):  
Ethyl Acetate ◽  
Cell Lines ◽  
Cancer Cell ◽  
Aerial Part ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Cancer Cell Lines ◽  
Imperata Cylindrica ◽  
Growth Inhibitory ◽  
Ht 29

Imperata cylindrica (L.) Raeusch. (IMP) aerial part ethyl acetate extract has anti-proliferative, pro-apoptotic, and pro-oxidative effects towards colorectal cancer in vitro. The chemical constituents of IMP aerial part ethyl acetate extract were isolated using high-performance liquid chromatography (HPLC) and identified with tandem mass spectrometry (ESI-MS/MS) in combination with ultraviolet-visible spectrophotometry and 400 MHz NMR. The growth inhibitory effects of each identified component on BT-549 (breast) and HT-29 (colon) cancer cell lines were evaluated after 48/72 h treatment by MTT assay. Four isolated compounds were identified as trans-p-Coumaric acid (1); 2-Methoxyestrone (2); 11, 16-Dihydroxypregn-4-ene-3, 20-dione (3); and Tricin (4). Compounds (2), (3), and (4) exhibited considerable growth inhibitory activities against BT-549 and HT-29 cancer cell lines. Compounds (2), (3), and (4) are potential candidates for novel anti-cancer agents against breast and colorectal cancers.

scholarly journals Acanthomine A, a new Pyrimidine-β-Carboline Alkaloid from the Sponge Acanthostrongylophora Ingens

2008 ◽  
Vol 3 (2) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Sabrin R. M. Ibrahim ◽  
RuAngelie Ebel ◽  
Rainer Ebel ◽  
Peter Proksch
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Brine Shrimp ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Chemical Investigation

Chemical investigation of the ethyl acetate extract of the sponge Acanthostrongylophora ingens afford one new pyrimidine-β-carboline alkaloid named acanthomine A (2), together with two known compounds annomontine (1) and 1,2,3,4-tetrahydronorharman-1-one (3). Their structures were unambiguously established on the basis of NMR spectroscopy (1H, 13C, 1H-1H COSY, HMQC and HMBC) and mass spectrometry. The isolated compounds were tested for cytotoxic activity using brine shrimp bioassay and different cancer cell lines.

Cytotoxic effects of novel polyoxotungstates and a platinum compound on human cancer cell lines

2005 ◽  
Vol 16 (1) ◽  
pp. 101-106 ◽  
Author(s):  
Hans U. V. Gerth ◽  
Annette Rompel ◽  
Bernt Krebs ◽  
Joachim Boos ◽  
Claudia Lanvers-Kaminsky
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Platinum Compound ◽  
Human Cancer Cell ◽  
Cytotoxic Effects ◽  
Human Cancer Cell Lines

scholarly journals Antimicrobial and Cytotoxic Effects of Mexican Medicinal Plants

2011 ◽  
Vol 6 (12) ◽  
pp. 1934578X1100601 ◽  
Author(s):  
Maria del Rosario Jacobo-Salcedo ◽  
Angel Josabad Alonso-Castro ◽  
Luis A. Salazar-Olivo ◽  
Candy Carranza-Alvarez ◽  
Luis Ángel González-Espíndola ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Cytotoxic Effects ◽  
Antimicrobial Effects ◽  
Human Cancer Cell Lines ◽  
Guazuma Ulmifolia

The antimicrobial effects of the Mexican medicinal plants Guazuma ulmifolia, Justicia spicigera, Opuntia joconostle, O. leucotricha, Parkinsonia aculeata, Phoradendron longifolium, P. serotinum, Psittacanthus calyculatus, Tecoma stans and Teucrium cubense were tested against several human multi-drug resistant pathogens, including three Gram (+) and five Gram (-) bacterial species and three fungal species using the disk-diffusion assay. The cytotoxicity of plant extracts on human cancer cell lines and human normal non-cancerous cells was also evaluated using the MTT assay. Phoradendron longifolium, Teucrium cubense, Opuntia joconostle, Tecoma stans and Guazuma ulmifolia showed potent antimicrobial effects against at least one multidrug-resistant microorganism (inhibition zone > 15 mm). Only Justicia spicigera and Phoradendron serotinum extracts exerted active cytotoxic effects on human breast cancer cells (IC50≤30 μg/mL). The results showed that Guazuma ulmifolia produced potent antimicrobial effects against Candida albicans and Acinetobacter lwoffii, whereas Justicia spicigera and Phoradendron serotinum exerted the highest toxic effects on MCF-7 and HeLa, respectively, which are human cancer cell lines. These three plant species may be important sources of antimicrobial and cytotoxic agents.

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