Application of Ionic Liquid-Based Ultrasonic-Assisted Extraction of Flavonoids from Bamboo Leaves

Ionic liquids (ILs), known as environmentally benign “green” solvents, were developed as an optimal solvent for the green extraction and separation field. In this paper, an ionic liquid-based ultrasonic-assisted extraction (IL-UAE) of flavonoids (FVs) from bamboo leaves of Phyllostachys heterocycla was developed for the first time. First, 1-butyl-3-methylimidazolium bromide ([Bmim] Br), with the best extraction efficiency, was selected from fifteen ionic liquids with diverse structure, like carbon chains or anions. Then, the influencing parameters of ionic liquid (IL) concentration, liquid-solid ratio, ultrasonic time, and ultrasonic power, were investigated by single factor tests, and further optimized using response surface methodology (RSM). In the optimization experiment, the best conditions were 1.5 mol/L [BMIM]Br aqueous solution, liquid-solid ratio 41 mL/g, ultrasonic time 90 min, and ultrasonic power 300 W. Furthermore, the microstructures of bamboo leaves and the recovery of FVs and [BMIM]Br were also studied. Therefore, this simple, green and effective IL-UAE method has potentiality for the extraction of FVs from bamboo leaves for the large-scale operations.

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Ultrasonic-Assisted Extraction of Procyanidins Using Ionic Liquid Solution fromLarix gmeliniiBark

Journal of Chemistry ◽

10.1155/2013/541037 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 8

Author(s):

Xiaowei Sun ◽

Zhimin Jin ◽

Lei Yang ◽

Jingwei Hao ◽

Yuangang Zu ◽

...

Keyword(s):

Ionic Liquid ◽

Ionic Liquids ◽

Extraction Efficiency ◽

Liquid Solution ◽

Extraction Process ◽

Ultrasonic Power ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

An ionic liquid-based ultrasonic-assisted extraction method has been developed for the effective extraction of procyanidins fromLarix gmeliniibark. So as to evaluate the performance of ionic liquids in ultrasonic-assisted extraction process, the effects caused by changes in the anion and the alkyl chain length of the cation on the extraction efficiency were investigated in this paper. The results indicated that the characteristics of anions had remarkable effects on the extraction efficiency of procyanidins, and 1-butyl-3-methylimidazolium bromide ([Bmim]Br) aqueous solution was the best among the investigated ionic liquids. The optimum conditions for the extraction were as follows: [Bmim]Br concentration 1.25 M, soak time 3 h, solid-liquid ratio 1 : 10, ultrasonic power 150 W, and ultrasonic time 30 min. This work not only introduces a simple, green, and highly efficient sample preparation method for extraction of procyanidins fromL. gmeliniibark, but also reveals the tremendous application potential of ionic liquids.

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Ionic Liquid-Based Ultrasonic-Assisted Extraction to Analyze Seven Compounds in Psoralea Fructus Coupled with HPLC

Molecules ◽

10.3390/molecules24091699 ◽

2019 ◽

Vol 24 (9) ◽

pp. 1699 ◽

Cited By ~ 5

Author(s):

Mengjun Shi ◽

Juanjuan Zhang ◽

Cunyu Liu ◽

Yiping Cui ◽

Changqin Li ◽

...

Keyword(s):

Ionic Liquid ◽

Extraction Yield ◽

Environmentally Benign ◽

Clinical Effects ◽

Ultrasonic Power ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction ◽

Solid Liquid

Psoralea Fructus is widely used in traditional Chinese medicine (TCM), and the content of psoralen, isopsoralen, neobavaisoflavone, bavachin, psoralidin, isobavachalcone, and bavachinin A is the main quality control index of Psoralea Fructus because of its clinical effects. Thus, a fast and environmentally-benign extraction method of seven compounds in Psoralea Fructus is necessary. In this work, an ionic liquid-based ultrasonic-assisted method (ILUAE) for the extraction of seven compounds from Psoralea Fructus was proposed. Several ILs of different types and parameters, including the concentration of ILs, concentration of ethanol (EtOH), solid–liquid ratio, particle size, ultrasonic time, centrifugal speed, and ultrasonic power, were optimized by the Placket–Burman (PB) design and Box–Behnken response surface analysis. Under this optimal condition, the total extraction yield of the seven compounds in Psoralea Fructus was 18.90 mg/g, and significantly greater than the conventional 75% EtOH solvent extraction.

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Separation of the main flavonoids and essential oil from seabuckthorn leaves by ultrasonic/microwave-assisted simultaneous distillation extraction

Royal Society Open Science ◽

10.1098/rsos.180133 ◽

2018 ◽

Vol 5 (7) ◽

pp. 180133 ◽

Cited By ~ 9

Author(s):

Chunying Li ◽

Jingjing Zhang ◽

Chunjian Zhao ◽

Lei Yang ◽

Wenyan Zhao ◽

...

Keyword(s):

Ionic Liquid ◽

Extraction Time ◽

Ultrasonic Power ◽

Solid Ratio ◽

Microwave Assisted ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Simultaneous Distillation Extraction ◽

Ultrasonic Assisted Extraction ◽

Optimized Conditions

Volatile essential oils (EOs), non-volatile rutin (RU), quercetin (QU), kaempferol (KA) and isorhamnetin (IS) were effectively extracted and isolated from seabuckthorn ( Hippophae rhamnoides L.) leaves by ionic liquid-based ultrasound/microwave-assisted simultaneous distillation extraction (ILUMASDE). After optimization by response surface methodology, EOs, RU, QU, KA and IS were separated under the following optimum conditions: an ionic liquid of 1.0 M 1-butyl-3-methyl imidazole bromine salt ([C4mim]), liquid/solid ratio of 12 ml g −1 , extraction time of 34 min, microwave power of 540 W and a fixed ultrasonic power of 50 W. Under the optimized conditions of ILUMASDE, the extraction yields of RU, QU, KA, IS and EOs were 9.18 ± 0.35, 5.52 ± 0.23, 3.03 ± 0.11, 5.64 ± 0.24 mg g −1 and 0.095 ± 0.004%, respectively. The yield of EOs obtained using ILUMASDE was 1.07-fold higher than that obtained by conventional hydrodistillation extraction (HDE). In addition, the components of the EOs obtained using ILUMASDE and HDE were similar. The extraction yields of RU, QU, KA, IS obtained by ILUMASDE were 1.03–1.35-fold higher than that obtained by the ethanol ultrasonic-assisted extraction (EUAE), ionic liquid-based ultrasonic-assisted extraction (ILUAE) and ionic liquid-based microwave-assisted extraction (ILMAE). And the extraction time used by ILUMASDE was 34 min, which is 14.17%, 56.67%, 56.67% and 85.00% less than those used by HDE, EUAE, ILUAE and ILMAE, respectively. Therefore, ILUMASDE can be considered a rapid and efficient method for extracting flavonoids and EO from seabuckthorn ( Hippophae rhamnoids L.) leaves.

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Optimization of Ultrasonic-Assisted Extraction, Characterization, and Antioxidant Activities of Polysaccharides From Sojae Semen Praeparatum

Natural Product Communications ◽

10.1177/1934578x211020622 ◽

2021 ◽

Vol 16 (5) ◽

pp. 1934578X2110206

Author(s):

Yongshuai Jing ◽

Ruijuan Zhang ◽

Lan Li ◽

Danshen Zhang ◽

Yu Liu ◽

...

Keyword(s):

Antioxidant Activities ◽

Deae Cellulose ◽

Chemical Compositions ◽

Hydroxyl Groups ◽

Ultrasonic Frequency ◽

Ultrasonic Power ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

In this study, response surface methodology (RSM) was used to optimize the ultrasonic-assisted extraction parameters of Sojae Semen Praeparatum polysaccharides (SSPP-80), the optimum conditions were determined as follows: ultrasonic frequency of 100 W, ultrasonic power of 80 Hz, ultrasonic temperature of 52℃, ultrasonic time of 23 minutes, and liquid to raw material ratio of 40 mL/g. Based on these conditions, polysaccharides extraction rate was 7.72% ± 0.26%. Then, 2 novel polysaccharides (SSPP-80‐1, SSPP-80‐2) were isolated from SSPP by DEAE-cellulose 52 chromatography. The chemical compositions, physicochemical properties, and structure of SSPPs were investigated by simultaneous thermal analyzer (TGA), scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FI-IR), and high-performance liquid chromatography (HPLC). The results showed that SSPP-80 and 2 fractions were mainly composed of mannose (Man), glucose (Glc), galactose (Gal), xylose (Xyl), and arabinose (Ara). In addition, the antioxidant activities were evaluated against the DPPH and hydroxyl radical in vitro, the IC50 of SSPP-80, SSPP-80‐1 and SSPP-80‐2 against DPPH free radical were 4.407, 8.267, and 5.204 mg/mL, respectively, whereas the IC50values for removing hydroxyl groups were 5.318, 3.516, and 4.016 mg/mL, respectively. It demonstrated that SSPP-80 and 2 fractions had certain antioxidant activity. Theoretical basis for use of Sojae Semen Praeparatum polysaccharides was provided by this study.

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Optimization of Ultrasonic-Assisted Extraction of Total Saponins from Ophiopholis mirabilis Using Response Surface Methodology

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.781-784.687 ◽

2013 ◽

Vol 781-784 ◽

pp. 687-693

Author(s):

Zhi Yang Shao ◽

Jin Xia Fu ◽

Yan Xia Qi ◽

Qian Cheng Zhao ◽

Zhi Bo Li

Keyword(s):

Response Surface Methodology ◽

Response Surface ◽

Ethanol Concentration ◽

Water Bath ◽

Ultrasonic Power ◽

Extraction Technology ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

Ultrasonic-Assisted extraction (UAE) was applied for saponins extraction from Ophiopholis mirabilis and response surface methodology (RSM) was used to optimize the extraction technology. A first set of experiments were designed to optimize water bath temperature, ethanol concentration, the ratio of liquid to solid, ultrasonic time (min) and ultrasonic power (W) as the main variables affecting the extraction effect. A Box-Behnken design consisting of 15 experimental runs was then applied and a second-order polynomial model was used to explain the interactions between the main factors and the yield of saponins. The developed mathematical model was found to fit well with the experimental data and the optimized conditions were ultrasonic pretreatment time 30.9 min, ethanol concentration 64%, liquid /solid ratio 35:1, ultrasonic power 300W and water bath 70°C. Corresponding saponins content was 0.574%.

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Optimization for the Process of Chlorophyll a Extraction by Orthogonal Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1051.423 ◽

2014 ◽

Vol 1051 ◽

pp. 423-427

Author(s):

Jun Sen Wu ◽

Chen Chen Liu ◽

Bing Li ◽

Xiu Zhi Wan

Keyword(s):

Chlorophyll A ◽

Orthogonal Experiment ◽

Ultrasonic Power ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction ◽

Extraction Agent ◽

Optimum Extraction ◽

Single Factor Experiment

The 90% acetone as extraction agent used in order to obtain the ultrasonic assisted extraction conditions of chlorophyll a. The ultrasonic power, ultrasonic time, centrifugation time and rotational speed as the four study factors and the chlorophyll a concentration as the target, here, the L9(34) orthogonal table of orthogonal experiment design are used in order to determine the optimum extraction contractions, basing on the single factor experiment. The experimental results shows the algae containing water can get better extraction effect, after ultrasonic 30 minutes in 225W and 6000r/min speed centrifugal 9 minutes and then mixing, repeat ultrasound centrifugation steps.

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Optimizing the Conditions for Polysaccharides Ultrasonic-Assisted Extraction in Mycelium of Paecilomyces Tenuipes Pt196 Using Statistical Approach

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.756-759.180 ◽

2013 ◽

Vol 756-759 ◽

pp. 180-185

Author(s):

Lin Na Du ◽

Wen Ting Bu ◽

Wen Lin Pan ◽

Chen Shao ◽

Jia Hui Lu ◽

...

Keyword(s):

Neural Network ◽

Genetic Algorithm ◽

Artificial Neural Network ◽

Ultrasonic Power ◽

Box Behnken Design ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Artificial Neural

In order to enhance the extraction rate of polysaccharides, a series of statistical approach was used to optimize the ultrasonic-assisted extraction conditions from the mycelium of Paecilomyces tenuipes Pt196. [Metho The optimization of conditions was carried out in two stages. Firstly, the effects of various experimental parameters considered for the investigation (ultrasonic power, ultrasonic time and liquid-solid ratio) were studied using the method of single factor test design experiments. Secondly, a 15-run Box-Behnken design was performed to optimize the extraction conditions of polysaccharides. The experimental results of Box-Behnken design were analyzed by response surface methodology and artificial neural network together with genetic algorithm. [Result The optimum conditions for polysaccharides extraction obtained by the application of artificial neural network-genetic algorithm were ultrasonic time 345s, ultrasonic power 320 W and liquid-solid ratio 95 mL·g-1. [Conclusion Artificial neural network-genetic algorithm can effectively select the best extraction conditions of polysaccharides in this study.

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Study on Extraction Process of Technics of Litsea Cubeba Oil

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.602-605.81 ◽

2014 ◽

Vol 602-605 ◽

pp. 81-85 ◽

Cited By ~ 1

Author(s):

Zuo Ren Liu ◽

Yan Fen Zhang ◽

Yong Guang Bi ◽

Hai Ling Li

Keyword(s):

Visual Analysis ◽

Extraction Process ◽

Oil Extraction ◽

Ultrasonic Power ◽

Extraction Temperature ◽

Litsea Cubeba ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

Litsea cubeba fruit as raw material in this experiment, the use of ultrasonic-assisted method of litsea cubeba oil extraction process are discussed. The effects of several ultrasonic processing parameters, such as ratio of liquid to solid, extraction temperature, ultrasonic power and extraction time on the extraction of litsea cubeba oil were investigated assisted by ultrasonic wave. And the L9 (34) orthogonal test was used to research this four factors, the result from the visual analysis indicated that the optimal extraction process was gotten when ratio of liquid to solid was 14:1 , ultrasonic time was 50min, ultrasonic power is 320W, ultrasonic temperature is 60 °C.Those experimental results showed that ultrasonic-assisted extraction of the Litsea cubeba oil is practicable, and this method is simple and efficient. With the preliminary investigation of the ultrasonic-assisted extraction of Litsea Cubeba oil, the oil yield reached up to 41.35%, this value is the highest of all the oil yields from the current oil extraction methods, and it is worthy of further exploration .

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Ultrasonic-Assisted Extraction of Natural Yellow Pigment from Physalis pubescens L. and Its Antioxidant Activities

Journal of Chemistry ◽

10.1155/2018/7861639 ◽

2018 ◽

Vol 2018 ◽

pp. 1-6

Author(s):

Haitang Wang ◽

Lin Shi ◽

Xiaoyu Yang ◽

Rui Hong ◽

Liang Li

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Yellow Pigment ◽

Ultrasonic Power ◽

Optimal Parameters ◽

Assisted Extraction ◽

Ultrasonic Time ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction

Physalis pubescens L. is rich in natural pigments but has not yet been fully utilized. Ultrasound-assisted extraction of yellow pigment from Physalis pubescens L. was investigated by response surface methodology in this study. Optimal parameters were ultrasonic power of 29.21%, ultrasonic time of 14.41 min, and ultrasonic interval time of 10.55 s. The yield was 0.193% under optimal parameters. FRAP, ABTS, and superoxide radical scavenging activity of the yellow pigment were 6.11 ± 0.22 mmol/g, 2.80 ± 0.27 mmol/g, and 57281.5 ± 2749.5 U/g, respectively. The results showed that the yield of yellow pigment could be improved by ultrasonic-assisted extraction and the yellow pigment extracted by ultrasound had antioxidant activity.

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Optimization of Ultrasonic-Assisted Extraction of Flavonols and Anthocyanins from Blueberry Using RSM

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.468-471.2423 ◽

2012 ◽

Vol 468-471 ◽

pp. 2423-2430 ◽

Cited By ~ 4

Author(s):

Yao Tian ◽

Yu Yang ◽

Ping Gao ◽

Jin Hong Wang ◽

Ya Qin Xu ◽

...

Keyword(s):

Response Surface Methodology ◽

Extraction Time ◽

Ultrasonic Power ◽

Experimental Conditions ◽

Independent Variables ◽

Solid Ratio ◽

Assisted Extraction ◽

Ultrasonic Assisted ◽

Ultrasonic Assisted Extraction ◽

Predicted Values

Ultrasonic-assisted extraction (UAE) method was developed for continued extraction of flavonols and anthocyanins from Blueberry. Response surface methodology (RSM) was employed to optimize experimental conditions for extraction of flavonols and anthocyanins. Three major independent variables such as ultrasonic power (W), extraction time (min), liquid to solid ratio (mL/g) were studied. The optimal extraction conditions of flavonols and anthocyanins were determined as follows: ultrasonic power 512.7 W, extraction time 29.8 min, liquid to solid ratio 9.5:1 mL /g. Under a modified condition (ultrasonic power 510W, extraction time 30 min, liquid to solid ratio 10:1 mL /g), the experimental yields of flavonols and anthocyanins were 0.806mg/g and 2.903mg/g respectively, which were agreed closely with the predicted values (0.804mg/g and 2.899mg/g).

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