scholarly journals Biological Effects of EF24, a Curcumin Derivative, Alone or Combined with Mitotane in Adrenocortical Tumor Cell Lines

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2202 ◽  
Author(s):  
Loris Bertazza ◽  
Susi Barollo ◽  
Maria Elena Mari ◽  
Irene Faccio ◽  
Maira Zorzan ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Biological Effects ◽  
Adrenocortical Tumor ◽  
Tumor Cell Lines ◽  
Adrenocortical Cancer ◽  
Reference Drug ◽  
Enhanced Solubility ◽  
H295r Cells ◽  
First Time

Background: Curcumin has numerous properties and is used in many preclinical conditions, including cancer. It has low bioavailability, while its derivative EF24 shows enhanced solubility. However, its effects have never been explored in adrenocortical tumor cell models. The efficacy of EF24 alone or combined with mitotane (reference drug for adrenocortical cancer) was evaluated in two adrenocortical tumor cell lines, SW13 and H295R. Method and Results: EF24 reduced cell viability with an IC50 (half maximal inhibitory concentration) of 6.5 ± 2.4 μM and 4.9 ± 2.8 μM for SW13 and H295R cells, respectively. Combination index (EF24 associated with mitotane) suggested an additivity effect in both cell lines. Cell cycle analysis revealed an increase in subG0/G1 phase, while motility assay showed a decrease in migratory cell capacity, and similarly, clonogenic assay indicated that EF24 could reduce colony numbers. Furthermore, Wnt/β-catenin, NF-κB, MAPK, and PI3k/Akt pathways were modulated by Western blot analysis when treating cells with EF24 alone or combined with mitotane. In addition, intracellular reactive oxygen species levels increased in both cell lines. Conclusion: This work analyzed EF24 in adrenocortical tumor cell lines for the first time. These results suggest that EF24 could potentially impact on adrenocortical tumors, laying the foundation for further research in animal models.

Biological Effects Induced by 68Ga-Conjugated Peptides in Human and Rodent Tumor Cell Lines

2018 ◽  
Vol 25 (3) ◽  
pp. 979-987
Author(s):  
Marieta Elena Panait ◽  
Livia Chilug ◽  
Valentina Negoita ◽  
Antonela Busca ◽  
Gina Manda ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Biological Effects ◽  
Tumor Cell Lines

l-Amino acid oxidase from Cerastes vipera snake venom: Isolation, characterization and biological effects on bacteria and tumor cell lines

Toxicon ◽  
2018 ◽  
Vol 150 ◽  
pp. 270-279 ◽  
Author(s):  
Walaa H. Salama ◽  
Nihal M. Ibrahim ◽  
Amr E. El Hakim ◽  
Roqaya I. Bassuiny ◽  
Manal M. Mohamed ◽  
...  
Keyword(s):  
Amino Acid ◽  
Tumor Cell ◽  
Cell Lines ◽  
Snake Venom ◽  
Biological Effects ◽  
Acid Oxidase ◽  
Tumor Cell Lines ◽  
Amino Acid Oxidase

Purification and differential biological effects of ginger-derived substances on normal and tumor cell lines

2012 ◽  
Vol 903 ◽  
pp. 157-162 ◽  
Author(s):  
James Almada da Silva ◽  
Amanda Blanque Becceneri ◽  
Hêmily Sanches Mutti ◽  
Ana Carolina Baptista Moreno Martin ◽  
Maria Fátima das Graças Fernandes Silva ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Biological Effects ◽  
Tumor Cell Lines

Parvifloracin and parviflorin: cytotoxic bistetrahydrofuran acetogenins with 35 carbons from Asimina parviflora (Annonaceae)

1994 ◽  
Vol 72 (2) ◽  
pp. 287-293 ◽  
Author(s):  
Sunil Ratnayake ◽  
Zhe-Ming Gu ◽  
Laura R. Miesbauer ◽  
David L. Smith ◽  
Karl V. Wood ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Test Data ◽  
Solid Tumor ◽  
Brine Shrimp ◽  
Tumor Cell Lines ◽  
Brine Shrimp Lethality ◽  
Human Solid Tumor ◽  
First Time

Using lethality to brine shrimp, activity-directed fractionation of extracts of Asimina parviflora (Michx.) Dunal. (Annonaceae) has led to the isolation of two new and four known acetogenins (1–6). Compound 1, parvifloracin, is the first nonadjacent bistetrahydrofuran acetogenin with 35 carbons. Compound 2, parviflorin, is an unusual adjacent bistetrahydrofuran acetogenin with 35 carbons. Bullatacin (3), molvizarin (4), annonacin (5), and goniothalamicin (6) are also reported for the first time from this plant. Brine shrimp lethality test data and comparative cytotoxicities of 1–6 against three human solid tumor cell lines are presented.

Polyprenylated Phloroglucinols from Hypericum maculatum

2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000
Author(s):  
Paraskev T. Nedialkov ◽  
Georgi Momekov ◽  
Zlatina K. Kokanova-Nedialkova ◽  
Jörg Heilmann
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Spectral Methods ◽  
Tumor Cell Lines ◽  
Aerial Parts ◽  
Phytochemical Investigation ◽  
Phloroglucinol Derivatives ◽  
New Compounds ◽  
First Time

A detailed phytochemical investigation of the dichloromethane extract of the aerial parts of Hypericum maculatum Crantz. led to the isolation of four new (2-5) and six known (1a/b, 6-10) polyprenylated phloroglucinol derivatives. The new compounds were identified by means of spectral methods (MS, NMR, IR, UV) as ( E)-4-(3,7-dimethylocta-2,6-dien-1-yl)-5-hydroxy-2-(3-methylbut-2-en-1-yl)-3,6-dioxocyclohexa-1,4-dien-1-yl isobutyrate (2), ( E)-2-(3,7-dimethylocta-2,6-dien-1-yl)-5-hydroxy-4-(3-methylbut-2-en-1-yl)-3,6-dioxocyclohexa-1,4-dien-1-yl isobutyrate (3), ( E)-4-(3,7-dimethylocta-2,6-dien-1-yl)-5-hydroxy-2-(3-methylbut-2-en-1-yl)-3,6-dioxocyclohexa-1,4-dien-1-yl 2-methylbutanoate (4) and ( E)-2-(3,7-dimethylocta-2,6-dien-1-yl)-5-hydroxy-4-(3-methylbut-2-en-1-yl)-3,6-dioxocyclohexa-1,4-dien-1-yl 2-methylbutanoate (5). The known compounds have been identified as hyperpolyphyllirin/hyperibine J (1a/b), erectquione A (6), ( E)-1-(3-(3,7-dimethylocta-2,6-dien-1-yl)-2,4,6-trihydroxyphenyl)-2-methylpropan-1-one (7), ( E)-1-(3-(3,7-dimethylocta-2,6-dien-1-yl)-2,4,6-trihydroxyphenyl)-2-methylbutan-1-one (8), 1-(5,7-dihydroxy-2-methyl-2-(4-methylpent-3-en-1-yl)chroman-8-yl)-2-methylpropan-1-one (9) and 1-(6,8-dihydroxy-1,1,4a-trimethyl-2,3,4,4a,9,9a-hexahydro-1H-xanthen-5-yl)-2-methylpropan-1-one (10). The stereochemistry of 1a is described for the first time. The cytotoxicity of 1-6 on SKW-3, BV-173 and K-562 tumor cell lines was determined using MTT based assays.

Interaction of Human Tumor Cells with Human Platelets and the Coagulation System

1983 ◽  
Vol 50 (03) ◽  
pp. 726-730 ◽  
Author(s):  
Hamid Al-Mondhiry ◽  
Virginia McGarvey ◽  
Kim Leitzel
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Cell Lines ◽  
Human Tumor ◽  
Human Platelets ◽  
Factor Vii ◽  
Coagulation System ◽  
Breast Adenocarcinoma ◽  
Activate Factor ◽  
Tumor Cell Lines

SummaryThis paper reports studies on the interaction between human platelets, the plasma coagulation system, and two human tumor cell lines grown in tissue culture: Melanoma and breast adenocarcinoma. The interaction was monitored through the use of 125I- labelled fibrinogen, which measures both thrombin activity generated by cell-plasma interaction and fibrin/fibrinogen binding to platelets and tumor cells. Each tumor cell line activates both the platelets and the coagulation system simultaneously resulting in the generation of thrombin or thrombin-like activity. The melanoma cells activate the coagulation system through “the extrinsic pathway” with a tissue factor-like effect on factor VII, but the breast tumor seems to activate factor X directly. Both tumor cell lines activate platelets to “make available” a platelet- derived procoagulant material necessary for the conversion of prothrombin to thrombin. The tumor-derived procoagulant activity and the platelet aggregating potential of cells do not seem to be inter-related, and they are not specific to malignant cells.

Differential Increases in Glutathione S-Transferase Activities in a Range of Multidrug-Resistant Human Tumor Cell Lines

1989 ◽  
Vol 1 (6) ◽  
pp. 359-365 ◽  
Author(s):  
Richard D. H. Whelan ◽  
Louise K. Hosking ◽  
Alan J. Townsend ◽  
Kenneth H. Cowan ◽  
Bridget T. Hill
Keyword(s):  
Tumor Cell ◽  
Cell Lines ◽  
Human Tumor ◽  
Multidrug Resistant ◽  
Tumor Cell Lines ◽  
Glutathione S Transferase ◽  
Human Tumor Cell ◽  
Human Tumor Cell Lines
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