scholarly journals Analysis of the Heterogeneous Distribution of Amiloride and Propranolol in Dried Blood Spot by UHPLC-FLD and MALDI-IMS

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4320
Author(s):  
Beatriz Uribe ◽  
Oskar González ◽  
María Encarnación Blanco ◽  
Oihane Elena Albóniga ◽  
María Luz Alonso ◽  
...  
Keyword(s):  
High Performance ◽  
Maldi Mass Spectrometry ◽  
Dried Blood Spot ◽  
Similar Distribution ◽  
Heterogeneous Distribution ◽  
Maldi Ims ◽  
Sampling Position ◽  
Analytical Response ◽  
Drug Dependent ◽  
Blood Spot

Dried blood spot (DBS) has lately experienced an increase in its use in bioanalysis due to its several advantages compared with traditional blood sampling methods. Nevertheless, the use of DBS with quantitative purposes is hindered by the heterogeneous distribution of some compounds in the supporting matrix and the dependence of the response on different factors, such as the hematocrit, blood volume, and sampling position. In this study the effect of those factors in the analytical response was investigated by ultra high performance liquid chromatography coupled to fluorescence detection, using amiloride and propranolol as model compounds. The results showed a heterogeneous and drug-dependent distribution of the compounds in the blood spot. While amiloride concentration was higher in the center, propranolol concentration was higher in the periphery of the spot. Besides, the influence of the hematocrit on the quantitative results was observed. MALDI mass spectrometry imaging (MALDI-IMS) has allowed study of the distribution of the two cardiovascular drugs when they were placed in the DBS card using water:methanol solutions, demonstrating that they followed a similar distribution pattern as in blood. This work has showed the potentiality of the MALDI-IMS technique to predict the distribution of the drugs in the DBS card.

scholarly journals Clinical Evaluation of a Dried Blood Spot Assay for Atazanavir

2010 ◽  
Vol 54 (10) ◽  
pp. 4124-4128 ◽  
Author(s):  
Trevor Van Schooneveld ◽  
Susan Swindells ◽  
Sarah R. Nelson ◽  
Brian L. Robbins ◽  
Ryan Moore ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Plasma Concentrations ◽  
Reversed Phase ◽  
Hiv Protease ◽  
Dried Blood Spot ◽  
Hiv Protease Inhibitors ◽  
Hiv Rna ◽  
Drug Concentrations ◽  
Blood Spot

ABSTRACT Current procedures for obtaining and measuring plasma concentrations of HIV protease inhibitors (PIs) are technically challenging. Dried blood spot (DBS) assays offer a way to overcome many of the obstacles. We sought to develop a DBS assay for quantitation of the PI atazanavir (ATV) and to compare this method with a previously validated plasma assay. We prospectively enrolled 48 patients with well-controlled HIV disease who had been on ATV for at least 7 days. ATV was quantified from plasma by use of high-performance liquid chromatography (HPLC). A reversed-phase ultrahigh-performance liquid chromatography (UPLC) assay was utilized for DBS samples. The concentrations of ATV quantified in a DBS matrix showed very strong agreement with those measured in plasma (r 2 = 0.988). The mean difference in ATV concentration between the two methods was −10.8% (95% confidence interval [95% CI], −7.65% to −13.95%), indicating that the DBS method has a slight negative bias. A majority (97.8%) of the differences in concentration between the two assays fell within ±2 standard deviations. ATV concentrations were lower in subjects who had detectable HIV RNA in plasma (mean, 543 ng/ml) than in those with HIV RNA of <50 copies/ml (mean, 1,582 ng/ml) (P = 0.03, Wilcoxon rank-sum test). In conclusion, our study demonstrated that ATV quantitation in a DBS matrix is feasible and accurate. DBS use offers a convenient alternative for measuring plasma concentrations of ATV and may have utility in monitoring of drug concentrations in clinical practice and in future studies.

scholarly journals Dried blood spot N-glycome analysis by MALDI mass spectrometry

Talanta ◽  
2019 ◽  
Vol 205 ◽  
pp. 120104 ◽  
Author(s):  
Gerda C.M. Vreeker ◽  
Marco R. Bladergroen ◽  
Simone Nicolardi ◽  
Wilma E. Mesker ◽  
Rob A.E.M. Tollenaar ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Maldi Mass Spectrometry ◽  
Dried Blood Spot ◽  
Blood Spot

scholarly journals VALIDATION OF 6-MERCAPTOPURINE AND 6-THIOGUANINE IN DRIED BLOOD SPOT BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY

2018 ◽  
Vol 10 (1) ◽  
pp. 412
Author(s):  
Yahdiana Harahap ◽  
Cheputri Rahma Astrini ◽  
Herman Suryadi
Keyword(s):  
Liquid Chromatography ◽  
Formic Acid ◽  
High Performance ◽  
Gradient Elution ◽  
Ultra Performance Liquid Chromatography ◽  
Dried Blood Spot ◽  
European Medicines Agency ◽  
Tandem Mass ◽  
Liquid Chromatography Tandem Mass ◽  
Blood Spot

Objective: This study aimed to obtain an optimal and validated method of analyzing 6-mercaptopurine (6-MP) and 6-thioguanine (6-TG) simultaneouslyin dried blood spot samples using ultra high-performance liquid chromatography-tandem mass spectrometry.Method: Separation was performed with a 1.7-μm amide column, which had a mobile phase with a flow rate of 0.2 mL/min and comprised 0.2%formic acid in water, 0.1% formic acid in acetonitrile, and methanol with a gradient elution. Detection was performed using Waters Xevo TQD.Result: This method was linear with a range of 25–1000 ng/mL for 6-MP and 6-TG, with consecutive r values of ≥0.996 and ≥0.995, respectively. Theintra- and inter-day % difference value and coefficient of variation for the accuracy and precision were not more than 15% and 20%, respectively, ata concentration lower limit of quantitation.Conclusion: This method fulfilled the requirements of the European Medicines Agency guideline for validation.

scholarly journals Pharmacokinetic Study and Incurred Sample Stability of Esomeprazole in Dried Blood Spot Sample Using High Performance Liquid Chromatography-Photodiode Array

2020 ◽  
Vol 20 (5) ◽  
pp. 979
Author(s):  
Yahdiana Harahap ◽  
Anja Tamabri ◽  
Vicha Vicha ◽  
Herman Suryadi ◽  
Sunarsih Sunarsih ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Pharmacokinetic Study ◽  
Healthy Subjects ◽  
High Performance ◽  
Dried Blood Spot ◽  
Sample Stability ◽  
Percent Difference ◽  
Photodiode Array ◽  
Blood Spot

In the past years, Esomeprazole (EMP) was analyzed in human plasma samples, which still has stability issues; thus, the new biosampling technique known as Dried Blood Spot (DBS) might solve the issue. This research aims to evaluate the incurred sample stability of esomeprazole in dried blood spot using high performance liquid chromatography-photodiode array with lansoprazole as an internal standard. The analytical separation was performed on a C-18 column (Waters, Sunfire™ 5 μm; 250 × 4.6 mm) at 40 °C. The mobile phase used was acetonitrile–phosphate buffer pH 7.6 (40:60% v/v) with a flow rate of 1.00 mL/min; and was detected at 300 nm. The analyte was extracted from dried blood spot by methanol. Incurred sample stability was evaluated from 6 healthy subjects on day 0, 7, 14, and 28, respectively. This method was linear in the range concentration of 70–1400 ng/mL with r > 0.98. Pharmacokinetic study shows that the average of AUC0–t of EMP in the DBS sample was 1765.41 ngh/mL. The highest percent difference value of esomeprazole’s incurred samples stability on day 7, 14, and 28 from 6 healthy subjects were 9.81%. This result fulfilled the acceptance criteria, which is the percent difference should not be greater than 20%, and 67% of total samples have to fulfill the criteria. The incurred sample stability result showed that esomeprazole was stable in the DBS sample at least until 28 days with the highest value of percent difference is 9.81%.

scholarly journals Metabolic profiles derived from residual blood spot samples: A longitudinal analysis

2018 ◽  
Vol 2 ◽  
pp. 28 ◽  
Author(s):  
Malia S.Q. Murphy ◽  
Steven Hawken ◽  
Wei Cheng ◽  
Lindsay A. Wilson ◽  
Monica Lamoureux ◽  
...  
Keyword(s):  
Newborn Screening ◽  
Gestational Age ◽  
High Performance ◽  
Intraclass Correlation ◽  
Estimation Algorithm ◽  
Dried Blood Spot ◽  
Sample Collection ◽  
Secondary Use ◽  
Blood Spot ◽  
Age Estimates

Background: Secondary use of newborn screening dried blood spot samples include use for biomedical or epidemiological research. However, the effects of storage conditions on archival samples requires further examination. The objective of this study was to determine the utility of residual newborn samples for deriving reliable metabolic gestational age estimates. Methods: Residual newborn dried blood spot samples that had been stored for 2-, 4-, 6-, or 12-months in temperature controlled (21°C) conditions were re-analyzed for the full panel of newborn screening analytes offered by a provincial newborn screening lab in Ottawa, Canada. Data from re-analyzed samples were compared to corresponding baseline newborn screening values for absolute agreement, and Pearson and intraclass correlation. Performance of a gestational age estimation algorithm originally developed from baseline newborn screening values was then validated on data derived from stored samples. Results: A total of 307 samples were used for this study. 17-hydroxyprogesterone and newborn hemoglobin profiles measured by immunoassay and high-performance liquid chromatography, respectively, were among the most stable markers across all time points of analysis. Acylcarnitines exhibited the greatest degree of variation in stability upon repeat measurement. The largest shifts in newborn analyte profiles and the poorest performance of metabolic gestational age algorithms were observed when samples were analyzed 12-months after sample collection. Conclusions: Duration of sample storage, independent of temperature and humidity, affects newborn screening profiles and gestational age estimates derived from metabolic gestational dating algorithms. When considering use of dried blood spot samples either for clinical or research purposes, care should be taken when interpreting data stemming from secondary use.

scholarly journals PHARMACOKINETIC PROFILE OF METFORMIN HYDROCHLORIDE IN DRIED BLOOD SPOT OF HEALTHY SUBJECTS USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY–PHOTODIODE ARRAY

2018 ◽  
Vol 10 (1) ◽  
pp. 354
Author(s):  
Yahdiana Harahap ◽  
Citra Dara Malia ◽  
Sunarsih .
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Healthy Subjects ◽  
High Performance ◽  
Pharmacokinetic Profile ◽  
Dried Blood Spot ◽  
Metformin Hydrochloride ◽  
Linear Calibration ◽  
Photodiode Array ◽  
Blood Spot

Objective: This study aimed to analyze the metformin hydrochloride in dried blood spot (DBS) sample and evaluate a pharmacokinetic profile in sixhealthy subjects who administered 850-mg metformin hydrochloride in tablet using high-performance liquid chromatography–photodiode array(HPLC–PDA).Methods: Metformin analysis was performed on data of six healthy subjects who administered 850 mg of metformin hydrochloride as tablets. Bloodsamples were taken at 12-time points at intervals of up to 12 h, and data were analyzed using HPLC–PDA.Results: A linear calibration curve was obtained in the range of 25–5000 ng/mL, with r=0.9990. Pharmacokinetic profiles of metformin were obtainedusing DBS samples from six healthy participants, with a Cmax of 347.3–416.22 ng/mL. The average tmax and t1/2 were obtained at 3 and 2 h, respectively,and the area under the curve (AUC)0-t/AUC0-∞ ratio was >80% for all subjects.Conclusion: The DBS biosampling technique can be developed for application in pharmacokinetic study of metformin HCl.

scholarly journals Measurement of Dabigatran Concentration Using Finger Prick Dried Blood Spot Sample Collection

2021 ◽  
Vol 12 ◽  
Author(s):  
Shin-Yi Lin ◽  
Yu-Fong Peng ◽  
Chih-Fen Huang ◽  
Ching-Hua Kuo ◽  
Sung-Chun Tang ◽  
...  
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
Dried Blood Spot ◽  
Altman Analysis ◽  
Sample Collection ◽  
Bland Altman Analysis ◽  
Clinical Scenarios ◽  
Deming Regression ◽  
Finger Prick ◽  
Blood Spot

Background and Purpose: Real-world laboratory monitoring of dabigatran activity is challenging. The purpose of the present study was to demonstrate the feasibility and accuracy of finger prick sampling with dried blood spot (fpDBS) cards in measuring the dabigatran concentration.Material and Methods: Patients &gt;20 years of age with atrial fibrillation and receiving dabigatran therapy for more than 7 days were included in the study. Peak and trough dabigatran concentrations were collected by simultaneous finger prick and venous puncture. The dabigatran concentration was measured by ultra-high performance liquid chromatography with tandem mass spectrometry. Our previously developed post-column infused internal standard (PCI-IS) method was applied to estimate the blood spot volume on fpDBS and to calibrate the drug concentration. Deming regression was used to analyze the correlation between dabigatran concentration on fpDBS cards and in plasma samples, followed by Bland–Altman analysis to compare the bias between two sampling techniques.Results: A total of 33 patients were enrolled and contributed 66 plasma and 55 fpDBS dabigatran samples. The average patient age was 74.6 ± 7.9 years, mean creatinine clearance 58.1 ± 18.3 mL/min, and CHA2DS2-VASc score 3.5 ± 1.6 points. The dabigatran concentration ranged from 41.8–1421.7 ng/mL. The plasma and DBS dabigatran concentrations correlated well (r = 0.98), and the conversion factor for fpDBS to plasma dabigatran concentration was 1.28. The Bland–Altman analysis showed that 94.5% of the fpDBS-predicted concentration fell within 20% of bias.Conclusions: The study showed that fpDBS measurement of dabigatran concentration is reliable and can be applied in clinical scenarios.

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