scholarly journals Antioxidant Effects and Phytochemical Properties of Seven Taiwanese Cirsium Species Extracts

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3935
Author(s):  
Zi-Wei Zhao ◽  
Hung-Chi Chang ◽  
Hui Ching ◽  
Jin-Cherng Lien ◽  
Hui-Chi Huang ◽  
...  

In the present investigation, we compared the radical-scavenging activities and phenolic contents of seven Taiwanese Cirsium species with a spectrophotometric method. We further analyzed their phytochemical profiles with high-performance liquid chromatography–photodiode array detection (HPLC–DAD). We found that the flower part of Cirsium japonicum var. australe (CJF) showed the best radical-scavenging activities against 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and the hypochlorite ion, for which the equivalents were 6.44 ± 0.17 mg catechin/g, 54.85 ± 0.66 mmol Trolox/g and 418.69 ± 10.52 mmol Trolox/g respectively. CJF also had the highest contents of total phenolics (5.23 ± 0.20 mg catechin/g) and phenylpropanoids (29.73 ± 0.72 mg verbascoside/g). According to the Pearson’s correlation coefficient, there was a positive correlation between the total phenylpropanoid content and ABTS radical-scavenging activities (r = 0.979). The radical-scavenging activities of the phenylpropanoids are closely related to their reducing power (r = 0.986). HPLC chromatograms obtained in validated HPLC conditions confirm that they have different phytochemical profiles by which they can be distinguished. Only CJF contained silicristin (0.66 ± 0.03 mg/g) and silydianin (9.13 ± 0.30 mg/g). CJF contained the highest contents of apigenin (5.56 ± 0.09 mg/g) and diosmetin (2.82 ± 0.10 mg/g). Among the major constituents, silicristin had the best radical-scavenging activities against DPPH (71.68 ± 0.66 mg catechin/g) and ABTS (3.01 ± 0.01 mmol Trolox/g). However, diosmetin had the best reducing power and radical-scavenging activity against the hypochlorite anion (41.57 ± 1.14 mg mmol Trolox/g). Finally, we found that flavonolignans (especial silicristin and silydianin) and diosmetin acted synergistically in scavenging radicals.

1992 ◽  
Vol 605 (2) ◽  
pp. 221-225 ◽  
Author(s):  
Marcello Previti ◽  
Francesco Dotta ◽  
Giuseppe Mario Pontieri ◽  
Umberto Di Mario ◽  
Luisa Lenti

2001 ◽  
Vol 67 (12) ◽  
pp. 5721-5728 ◽  
Author(s):  
Alice C. L. Churchill ◽  
Larry D. Dunkle ◽  
Walter Silbert ◽  
Kevin J. Kennedy ◽  
Vlado Macko

ABSTRACT Pathogenic strains of the soilborne fungus Periconia circinata produce peritoxins with host-selective toxicity against susceptible genotypes of sorghum. The peritoxins are low-molecular-weight, hybrid molecules consisting of a peptide and a chlorinated polyketide. Culture fluids from pathogenic, toxin-producing (Tox+) and nonpathogenic, non-toxin-producing (Tox−) strains were analyzed directly by gradient high-performance liquid chromatography (HPLC) with photodiode array detection and HPLC-mass spectrometry to detect intermediates and final products of the biosynthetic pathway. This approach allowed us to compare the metabolite profiles of Tox+ and Tox− strains. Peritoxins A and B and the biologically inactive intermediates,N-3-(E-pentenyl)-glutaroyl-aspartate, circinatin, and 7-chlorocircinatin, were detected only in culture fluids of the Tox+ strains. The latter two compounds were produced consistently by Tox+ strains regardless of the amount of peritoxins produced under various culture conditions. In summary, none of the known peritoxin-related metabolites were detected in Tox− strains, which suggests that these strains may lack one or more functional genes required for peritoxin biosynthesis.


Sign in / Sign up

Export Citation Format

Share Document