scholarly journals An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4647
Author(s):  
Meng Wang ◽  
Yirong Zhang ◽  
Ruijie Wang ◽  
Zhibin Wang ◽  
Bingyou Yang ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ex Situ ◽  
Research Progress ◽  
Antioxidant Compounds ◽  
Detection Techniques ◽  
Tlc Bioautography ◽  
Detection Technology ◽  
Layer Chromatography

Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determining antibacterial, antifungal, antitumor and antioxidant compounds and for inhibiting glucosidase, pancreatic lipase, tyrosinase and cholinesterase activity constitutes. Nowadays, especially during the COVID-19 pandemic, it is important to identify original, natural products with anti-COVID potential compounds from Chinese traditional medicine and natural medicinal plants. We also give an account of detection techniques, including in situ and ex situ techniques; even in situ ion sources represent a major reform. Considering the current technical innovations, we propose that the technology will make more progress in TLC plates with higher separation and detection technology with a more portable and extensive scope of application. We believe this technology will be diffusely applied in medicine, biology, agriculture, animal husbandry, garden forestry, environmental management and other fields in the future.

Thin-layer chromatography with in situ multichannel image detection of fluorescent compounds

1981 ◽  
Vol 53 (9) ◽  
pp. 1357-1361 ◽  
Author(s):  
M. L. Gianelli ◽  
J. B. Callis ◽  
G. D. Christian ◽  
N. H. Andersen ◽  
G. D. Christian
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Image Detection ◽  
Layer Chromatography ◽  
Fluorescent Compounds

Quantitative in situ thin-layer chromatography of ergocalciferol in multivitamin tablets

1978 ◽  
Vol 11 (4) ◽  
pp. 217-219 ◽  
Author(s):  
M. Širec ◽  
Lj. Mikša ◽  
T. Bičan-Fišter ◽  
M. Prošek ◽  
E. Kučan
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography

Microchannel Thin Layer Chromatography with in situ Plate Scanning Micro-DRIFTS Detection Using Plain and Polybutadiene Modified Zirconia Stationary Phases

2002 ◽  
Vol 56 (8) ◽  
pp. 1059-1066 ◽  
Author(s):  
Beata A. Musial ◽  
André J. Sommer ◽  
Neil D. Danielson
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Diffuse Reflectance ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Diffuse Reflectance Infrared ◽  
Layer Chromatography ◽  
Tlc Separation ◽  
Zirconia Stationary Phases

Thin layer chromatography (TLC) of various dyes is compared in microchannels packed with either bare zirconia (normal phase) or polybutadiene (PBD) modified zirconia (reversed phase). In situ micro-diffuse reflectance infrared Fourier transform spectroscopy (μDRIFTS) of the analyte spots is possible due to the low absorption background of both plain and PBD zirconia. An instrument coupling μDRIFTS with a motorized stage that could profile the microchannel TLC plate is developed. The retention order of anthracene and pyrene probes as a function of percent acetonitrile are generally as expected on PBD zirconia but opposite for plain zirconia. Impurities in technical grade methylene blue can be determined after TLC separation on plain zirconia. The separation and infrared identification of various dyes such as dichlorofluorescein and rhodamine B on PBD zirconia is improved using a mobile phase with an additive such as phosphate or dihexylamine that prevents band streaking.

Detection of in situ Compound Instability by Two-Dimensional Thin-layer Chromatography

1966 ◽  
Vol 55 (4) ◽  
pp. 429-430 ◽  
Author(s):  
Melvin H. Penner ◽  
Joseph M. Talmage ◽  
Milton Geller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Two Dimensional ◽  
Layer Chromatography

SEPARATION AND CHARACTERISATION OF Δ4-3-KETOSTEROIDS OF THE PREGNANE-SERIES BY MEANS OF THIN-LAYER CHROMATOGRAPHY

1963 ◽  
Vol 43 (1) ◽  
pp. 47-66 ◽  
Author(s):  
B. P. Lisboa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Thinlayer Chromatography ◽  
Pregnane Series ◽  
Layer Chromatography

ABSTRACT A method is described for the separation and characterisation of thirty-two Δ4-3-ketosteroids of the pregnane-series. The method involves thinlayer chromatography on Silica Gel G of the various steroids and of various derivatives thereof, followed by seven in situ colour reactions. Data are presented on the reproducibility of the method.

scholarly journals Thin-Layer Chromatography-Densitometry and Liquid Chromatography Analysis of Alkaloids in Leaves of Papaver somniferum Under Stress Conditions

2005 ◽  
Vol 88 (5) ◽  
pp. 1571-1578 ◽  
Author(s):  
Beata Szabó ◽  
Ágnes Lakatos ◽  
Tamás Kõszegi ◽  
György Kátay ◽  
L Botz
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Developmental Stages ◽  
Papaver Somniferum ◽  
Stress Conditions ◽  
Chromatography Method ◽  
Detection Techniques ◽  
Layer Chromatography ◽  
Alkaloid Contents

Abstract The effect of stress conditions on the concentrations of secondary metabolites were examined during various developmental stages of Papaver somniferum plants. P. somniferum plants were grown in laboratory conditions (Budakalász). The experiment consisted of 22 treatments. Significantly different alkaloid contents can be observed under different stress conditions. In general, the alkaloid contents of plants are very low; therefore, a highly sensitive and reliable method has to be developed for analysis. The amount of alkaloids was measured by 2 separation and detection techniques. Accuracy of the thin-layer chromatography method for quantitative analysis is limited. Without purification of samples the background is too noisy. Column liquid chromatography is a sensitive and relatively inexpensive method that allows precise quantitative determination of the alkaloid content.

Sign in / Sign up

Export Citation Format

Share Document