Cellular Responses of Human Lymphatic Endothelial Cells to Carbon Nanomaterials

One of the greatest challenges to overcome in the pursuit of the medical application of carbon nanomaterials (CNMs) is safety. Particularly, when considering the use of CNMs in drug delivery systems (DDSs), evaluation of safety at the accumulation site is an essential step. In this study, we evaluated the toxicity of carbon nanohorns (CNHs), which are potential DDSs, using human lymph node endothelial cells that have been reported to accumulate CNMs, as a comparison to fibrous, multi-walled carbon nanotubes (MWCNTs) and particulate carbon black (CB). The effect of different surface characteristics was also evaluated using two types of CNHs (untreated and oxidized). In the fibrous MWCNT, cell growth suppression, as well as expression of inflammatory cytokine genes was observed, as in previous reports. In contrast, no significant toxicity was observed for particulate CB and CNHs, which was different from the report of CB cytotoxicity in vascular endothelial cells. These results show that (1) lymph endothelial cells need to be tested separately from other endothelial cells for safety evaluation of nanomaterials, and (2) the potential of CNHs as DDSs.

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A rhenium review – from discovery to novel applications

Archives of Materials Science and Engineering ◽

10.5604/01.3001.0009.7106 ◽

2016 ◽

Vol 82 (2) ◽

pp. 70-78 ◽

Cited By ~ 8

Author(s):

A.D. Dobrzańska-Danikiewicz ◽

W. Wolany

Keyword(s):

Carbon Nanotubes ◽

Carbon Nanomaterials ◽

Electrical Equipment ◽

Advanced Materials ◽

Market Demand ◽

Carbon Nanohorns ◽

Single Walled Carbon ◽

Multi Walled Carbon Nanotubes ◽

Rhenium Alloy ◽

Walled Carbon Nanotubes

Purpose: The article characterises rhenium in terms of its physiochemical properties,most popular methods of manufacturing and key applications. The examples of rhenium ata nanometric scales are also presented, taking into account the latest literature reports inthis field. The objective of the article is also to present advanced nanocomposite materialsconsisting of nanostructured rhenium permanently attached to selected carbon nanomaterials- Single Walled Carbon NanoTubes (SWCNTs), Double Walled Carbon NanoTubes (DWCNTs),Multi Walled Carbon NanoTubes (MWCNTs) and Single Walled Carbon Nanohorns (SWCNHs).Design/methodology/approach: The article delineates various manufacturing methodsat a mass and nanometric scale. It also describes a custom fabrication method of carbonrheniumnanocomposites and the results of investigations performed in a transmissionelectron microscope (TEM) for nanocomposites of the following type: MWCNTs-Re,SWCNTs/DWCNTs-Re, SWCNTs-Re and SWCNHs-Re.Findings: Rhenium has been gaining growing importance in industry for years, and itsapplications are very diverse, including: heat resistant alloys, anti-corrosive alloys, rheniumand rhenium alloy coatings, elements of electrical equipment, radiotherapy, chemistry andanalytical technology and catalysis. Carbon-metallic nanocomposites are currently enjoyingstrong attention of research institutions.Research limitations/implications: The development and optimisation of fabricationprocesses of materials containing carbon nanotubes or carbon nanotubes coated with metalnanoparticles, especially rhenium, is a weighty aspect of advanced materials engineering.Practical implications: Newly created nanocomposite materials, developed as a responseto the market demand, are interesting, state-of-the-art materials dedicated to variousapplications, especially as gas or fluid sensors, and as materials possessing catalytic properties.Originality/value: The article describes nanocomposites of the following types: MWCNTs-Re, SWCNTs/DWCNTs-Re, SWCNTs-Re, SWCNHs-Re, created as a result of hightemperaturereduction of a precursor of rhenium (HReO4 or NH4ReO4) to metallic rhenium.This metal is deposited on carbon nanomaterials as nanoparticles, or inside of them asnanoparticles or nanowires whose size and dispersion are dependent upon the conditionsof a technological process.

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M.629 Homocysteine inhibits lysyl oxidase (LOX) and downregulates LOX expression in vascular endothelial cells

Atherosclerosis ◽

10.1016/s0021-9150(04)90627-2 ◽

2004 ◽

Vol 5 (1) ◽

pp. 146

Author(s):

B RAPOSO

Keyword(s):

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Lysyl Oxidase ◽

Vascular Endothelial

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Anti-metalloproteinase-9 Activities of Selected Indonesian Zingiberaceae Rhizome Extracts in Lipopolysaccharide-induced Human Vascular Endothelial Cells In Vitro

Planta Medica ◽

10.1055/s-0031-1282610 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

Y Yanti ◽

N Steven ◽

A Wiharja ◽

S Fajarianto

Keyword(s):

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Vascular Endothelial ◽

Metalloproteinase 9

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The Endothelial Cell and the Factor VIII Bypassing Activity of Prothrombin Complex Concentrate

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647034 ◽

1988 ◽

Vol 60 (02) ◽

pp. 226-229 ◽

Cited By ~ 2

Author(s):

Jerome M Teitel ◽

Hong-Yu Ni ◽

John J Freedman ◽

M Bernadette Garvey

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Factor Viii ◽

Prothrombin Complex Concentrate ◽

Vascular Endothelial Cells ◽

Vascular Endothelial ◽

Monolayer Cultures ◽

Coagulant Activity ◽

Time Courses ◽

Privileged Site

SummarySome classical hemophiliacs have a paradoxical hemostatic response to prothrombin complex concentrate (PCC). We hypothesized that vascular endothelial cells (EC) may contribute to this “factor VIII bypassing activity”. When PCC were incubated with suspensions or monolayer cultures of EC, they acquired the ability to partially bypass the defect of factor VIII deficient plasma. This factor VIII bypassing activity distributed with EC and not with the supernatant PCC, and was not a general property of intravascular cells. The effect of PCC was even more dramatic on fixed EC monolayers, which became procoagulant after incubation with PCC. The time courses of association and dissociation of the PCC-derived factor VIII bypassing activity of fixed and viable EC monolayers were both rapid. We conclude that EC may provide a privileged site for sequestration of constituents of PCC which express coagulant activity and which bypass the abnormality of factor VIII deficient plasma.

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Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

Thrombosis and Haemostasis ◽

10.1055/s-0038-1649879 ◽

1995 ◽

Vol 74 (04) ◽

pp. 1045-1049 ◽

Cited By ~ 38

Author(s):

P Butthep ◽

A Bunyaratvej ◽

Y Funahara ◽

H Kitaguchi ◽

S Fucharoen ◽

...

Keyword(s):

Endothelial Cells ◽

Endothelial Cell ◽

Immunosorbent Assay ◽

Plasma Proteins ◽

Clinical Symptoms ◽

Vascular Endothelial Cells ◽

Vascular Endothelial Cell ◽

Enzyme Linked Immunosorbent Assay ◽

Vascular Endothelial ◽

Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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Modulation of Arachidonic Acid Metabolism in Human Endothelial Cells by Glucocorticoids

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661566 ◽

1986 ◽

Vol 55 (03) ◽

pp. 369-374 ◽

Cited By ~ 15

Author(s):

Raffaele De Caterina ◽

Babette B Weksler

Keyword(s):

Endothelial Cells ◽

Vascular Endothelial Cells ◽

Umbilical Vein ◽

Arachidonic Acid Metabolism ◽

Protein Synthesis Inhibitor ◽

Synthesis Inhibitor ◽

Prolonged Incubation ◽

Dependent Inhibition ◽

Human Thrombin ◽

Umbilical Vein Endothelial Cells

SummaryTo learn whether glucocorticoids inhibit prostaglandin (PG) production in vascular endothelial cells, we investigated the effects of glucocorticoids on PG synthesis by cultured human umbilical vein endothelial cells (EC). Pretreatment of EC with dexamethasone (DX, 10-9 to 5 x 10-5 M) caused a dose-dependent inhibition of PGI2 production when PG synthesis from endogenous arachidonate was stimulated by human thrombin (0.25-2 U/ml) or ionophore A 23187 (1-5 μM). The inhibition was detectable at 10-7 M DX and maximal at 10-5 M (4.0 ± 0.7 vs. control: 7.7 ± 1.9 ng/ml, mean ± S.D., P <0.01). The production of PGE2 and the release of radiolabelled arachidonate (AA) from prelabelled cells were similarly inhibited. Prolonged incubation of EC with glucocorticoids was required to inhibit PG production or arachidonate release: ranging from 8% inhibition at 5 h to 44% at 38 h. In contrast, prostaglandin formation from exogenous AA was not altered by DX treatment. When thrombin or ionophore-stimulated EC were restimulated with exogenous AA (25 μM), DX-treated cells released more PGI2 than control cells (5.7 ± 0.5 vs. 4.1 ± 0.6 ng/ml, P <0.01). Both the decrease in PGI2 production after thrombin/ionophore and the increase after re-stimulation with AA were blunted in the presence of the protein synthesis inhibitor cycloheximide (0.1-0.2 μg/ml). Thus, incubation of EC with glucocorticoids inhibits PG production at the step of phospholipase activation. The time requirement for these steroid effects and their blunting by cycloheximide are consistent with the induction of regulatory proteins, possibly lipocortins, in endothelial cells.

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