PNA-Based Graphene Oxide/Porous Silicon Hybrid Biosensor: Towards a Label-Free Optical Assay for Brugada Syndrome

Peptide nucleic acid (PNA) is a synthetic DNA mimic that outperforms the properties of traditional oligonucleotides (ONs). On account of its outstanding features, such as remarkable binding affinity towards complementary DNA or RNA as well as high thermal and chemical stability, PNA has been proposed as a valuable alternative to the ON probe in gene-sensor design. In this study, a hybrid transducer made-up of graphene oxide (GO) nano-sheets covalently grafted onto a porous silicon (PSi) matrix has been investigated for the early detection of a genetic cardiac disorder, the Brugada syndrome (BS). A functionalization strategy towards the realization of a potential PNA-based device is described. A PNA, able to detect the SCN5A gene associated with the BS, has been properly synthesized and used as a bioprobe for the realization of a proof-of-concept label-free optical PNA-biosensor. PSi reflectance and GO photoluminescence signals were simultaneously exploited for the monitoring of the device functionalization and response.

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PNA-based Graphene Oxide/porous Silicon Hybrid Biosensor: Towards a Label-free Optical Assay for Brugada Syndrome

10.20944/preprints202011.0027.v1 ◽

2020 ◽

Author(s):

Rosalba Moretta ◽

Monica Terracciano ◽

Nicola Borbone ◽

Giorgia Oliviero ◽

Chiara Schiattarella ◽

...

Keyword(s):

Graphene Oxide ◽

Nucleic Acid ◽

Porous Silicon ◽

Brugada Syndrome ◽

Peptide Nucleic Acid ◽

Proof Of Concept ◽

Label Free ◽

Synthetic Dna ◽

Cardiac Disorder ◽

Optical Assay

Peptide nucleic acid (PNA) is a synthetic DNA mimic that outperforms the properties of traditional oligonucleotides (ONs). On account of its outstanding features, such as remarkable binding affinity towards complementary DNA or RNA as well as high thermal and chemical stability, PNA has been proposed as a valuable alternative to the ON probe in gene-sensor design. In this study, a hybrid transducer made-up of graphene oxide (GO) nano-sheets covalently grafted onto a porous silicon (PSi) matrix has been investigated for the early detection of a genetic cardiac disorder, the Brugada syndrome (BS). A functionalization strategy towards the realization of a potential PNA-based device is described. A peptide nucleic acid (PNA), able to detect the SCN5A associated with the BS has been properly synthesized and used as a bioprobe for the realization of a proof-of-concept label-free optical PNA-biosensor. PSi reflectance and GO photoluminescence (PL) signals were simultaneously exploited for the monitoring of the device functionalization and response.

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Rapid and label-free optical assay of S-layer protein with high sensitivity using TiO2-coated porous silicon-based microfluidic biosensor

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2020.128524 ◽

2020 ◽

Vol 321 ◽

pp. 128524 ◽

Cited By ~ 1

Author(s):

Siqi Zhuo ◽

Mingyue Xun ◽

Mingda Li ◽

Xiaoxue Kong ◽

Rui Shao ◽

...

Keyword(s):

Porous Silicon ◽

High Sensitivity ◽

Label Free ◽

Microfluidic Biosensor ◽

Optical Assay ◽

Silicon Based

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Isothermal Detection of Canine Blood Parasite (Ehrlichia canis) Utilizing Recombinase Polymerase Amplification Coupled with Graphene Oxide Quenching-Based Pyrrolidinyl Peptide Nucleic Acid

Bioconjugate Chemistry ◽

10.1021/acs.bioconjchem.0c00639 ◽

2021 ◽

Author(s):

Phanomsak Yukhet ◽

Kittisak Buddhachat ◽

Tirayut Vilaivan ◽

Chaturong Suparpprom

Keyword(s):

Graphene Oxide ◽

Nucleic Acid ◽

Peptide Nucleic Acid ◽

Blood Parasite ◽

Ehrlichia Canis ◽

Recombinase Polymerase Amplification

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Chemical binding of pyrrolidinyl peptide nucleic acid ( acpcPNA‐T9 ) probe with AuNPs toward label‐free monitoring of miRNA ‐21: A novel biosensing platform for biomedical analysis and POC diagnostics

Journal of Molecular Recognition ◽

10.1002/jmr.2893 ◽

2021 ◽

Author(s):

Nazanin Fathi ◽

Arezoo Saadati ◽

Mohammad Hasanzadeh ◽

Mohammad Samiei

Keyword(s):

Nucleic Acid ◽

Peptide Nucleic Acid ◽

Label Free ◽

Biomedical Analysis ◽

Chemical Binding

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Novel SCN5A p.Val1667Asp Missense Variant Segregation and Characterization in a Family with Severe Brugada Syndrome and Multiple Sudden Deaths

International Journal of Molecular Sciences ◽

10.3390/ijms22094700 ◽

2021 ◽

Vol 22 (9) ◽

pp. 4700

Author(s):

Michelle M. Monasky ◽

Emanuele Micaglio ◽

Giuseppe Ciconte ◽

Ilaria Rivolta ◽

Valeria Borrelli ◽

...

Keyword(s):

Genetic Testing ◽

Family History ◽

Risk Stratification ◽

Brugada Syndrome ◽

Electrophysiological Study ◽

Functional Characterization ◽

The Family ◽

Novel Variant ◽

History Of ◽

Scn5a Gene

Genetic testing in Brugada syndrome (BrS) is still not considered to be useful for clinical management of patients in the majority of cases, due to the current lack of understanding about the effect of specific variants. Additionally, family history of sudden death is generally not considered useful for arrhythmic risk stratification. We sought to demonstrate the usefulness of genetic testing and family history in diagnosis and risk stratification. The family history was collected for a proband who presented with a personal history of aborted cardiac arrest and in whom a novel variant in the SCN5A gene was found. Living family members underwent ajmaline testing, electrophysiological study, and genetic testing to determine genotype-phenotype segregation, if any. Patch-clamp experiments on transfected human embryonic kidney 293 cells enabled the functional characterization of the SCN5A novel variant in vitro. In this study, we provide crucial human data on the novel heterozygous variant NM_198056.2:c.5000T>A (p.Val1667Asp) in the SCN5A gene, and demonstrate its segregation with a severe form of BrS and multiple sudden deaths. Functional data revealed a loss of function of the protein affected by the variant. These results provide the first disease association with this variant and demonstrate the usefulness of genetic testing for diagnosis and risk stratification in certain patients. This study also demonstrates the usefulness of collecting the family history, which can assist in understanding the severity of the disease in certain situations and confirm the importance of the functional studies to distinguish between pathogenic mutations and harmless genetic variants.

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Repeated molecular genetic analysis in Brugada syndrome revealed a novel disease-associated large deletion in the SCN5A gene

HeartRhythm Case Reports ◽

10.1016/j.hrcr.2016.02.008 ◽

2016 ◽

Vol 2 (3) ◽

pp. 261-264 ◽

Cited By ~ 2

Author(s):

Anders Krogh Broendberg ◽

Lisbeth Noerum Pedersen ◽

Jens Cosedis Nielsen ◽

Henrik Kjaerulf Jensen

Keyword(s):

Genetic Analysis ◽

Brugada Syndrome ◽

Molecular Genetic ◽

Large Deletion ◽

Molecular Genetic Analysis ◽

Scn5a Gene

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Role of Adsorptive Concentration in Fenton‐Like Degradation of Organic Pollutants by Biopolymeric FeOOH/Graphene Oxide Composite Catalyst: Proof of Concept

Advanced Sustainable Systems ◽

10.1002/adsu.202100060 ◽

2021 ◽

pp. 2100060

Author(s):

Amit Kumar Sarkar ◽

Yeoung‐Sang Yun

Keyword(s):

Graphene Oxide ◽

Organic Pollutants ◽

Composite Catalyst ◽

Proof Of Concept ◽

Oxide Composite ◽

Adsorptive Concentration

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Label-free electrochemical immunosensor based on gold nanoparticle/polyethyleneimine/reduced graphene oxide nanocomposite for the ultrasensitive detection of cancer biomarker matrix metalloproteinase-1

The Analyst ◽

10.1039/d1an00537e ◽

2021 ◽

Author(s):

Xinke Liu ◽

Lu-Yin Lin ◽

Fu-Yen Tseng ◽

Yu-Cheng Tan ◽

Jian Li ◽

...

Keyword(s):

Graphene Oxide ◽

Matrix Metalloproteinase ◽

Gold Nanoparticle ◽

Reduced Graphene Oxide ◽

Electrochemical Immunosensor ◽

Ultrasensitive Detection ◽

Label Free ◽

Matrix Metalloproteinase 1 ◽

Reduced Graphene ◽

P Matrix

Matrix metalloproteinase-1 (MMP-1) is associated with many types of cancers, including oral, colorectal, and brain cancers. This paper describes the fabrication of an MMP-1 immunosensor based on a gold nanoparticle/polyethyleneimine/reduced...

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