scholarly journals Robust Copper Metal–Organic Framework-Embedded Polysiloxanes for Biomedical Applications: Its Antibacterial Effects on MRSA and In Vitro Cytotoxicity

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 719
Author(s):  
Kihak Gwon ◽  
Youngmee Kim ◽  
Hyunjun Cho ◽  
Seonhwa Lee ◽  
So-Hyeon Yang ◽  
...  

Polysiloxanes (PSs) have been widely utilized in the industry as lubricants, varnishes, paints, release agents, adhesives, and insulators. In addition, their applications have been expanded to include the development of new biomedical materials. To modify PS for application in therapeutic purposes, a flexible antibacterial Cu-MOF (metal–organic framework) consisting of glutarate and 1,2-bis(4-pyridyl)ethane ligands was embedded in PS via a hydrosilylation reaction of vinyl-terminated and H-terminated PSs at 25 °C. The bactericidal activities of the resulting Cu-MOF-embedded PS (PS@Cu-MOF) and the control polymer (PS) were tested against Escherichia coli, Staphylococcus aureus, and methicillin-resistant Staphylococcus aureus. PS@Cu-MOF exhibited more than 80% bactericidal activity toward the tested bacteria at a concentration of 100 μg⋅mL−1 and exhibited a negligible cytotoxicity toward mouse embryonic fibroblasts at the same concentration. Release tests of the Cu(II) ion showed PS@Cu-MOF to be particularly stable in a phosphate-buffered saline solution. Furthermore, its physical and thermal properties, including the phase transition, rheological measurements, swelling ratio, and thermogravimetric profile loss, were similar to those of the control polymer. Moreover, the low cytotoxicity and bactericidal activities of PS@Cu-MOF render it a promising candidate for use in medicinal applications, such as in implants, skin-disease treatment, wound healing, and drug delivery.

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1211 ◽  
Author(s):  
Gongsen Chen ◽  
Xin Leng ◽  
Juyuan Luo ◽  
Longtai You ◽  
Changhai Qu ◽  
...  

A MIL series metal‒organic framework (MOF), MIL-100(Fe), was successfully synthesized at the nanoscale and fully characterized by TEM, TGA, XRD, FTIR, DLS, and BET. A toxicological assessment was performed using two different cell lines: human normal liver cells (HL-7702) and hepatocellular carcinoma (HepG2). In vitro cytotoxicity of MIL-100(Fe) was evaluated by the MTT assay, LDH releasing rate assay, DAPI staining, and annexin V/PI double staining assay. The safe dose of MIL-100(Fe) was 80 μg/mL. It exhibited good biocompatibility, low cytotoxicity, and high cell survival rate (HL-7702 cells’ viability >85.97%, HepG2 cells’ viability >91.20%). Therefore, MIL-100(Fe) has a potential application as a drug carrier.


Author(s):  
Shi-Chao Qi ◽  
Zhi-Hui Yang ◽  
Rong-Rong Zhu ◽  
Xiao-Jie Lu ◽  
Ding-Ming Xue ◽  
...  

Reproducing the highly efficient catalytic cascade system to mimic life behaviors in vitro has been intensively investigated over the past few decades. However, except the biocatalysis with enzymes, most chemocatalytic...


2020 ◽  
Vol 10 (6) ◽  
pp. 934-941 ◽  
Author(s):  
Dongming Wang ◽  
Xiaoliu Liu ◽  
Xiaomei Ye ◽  
Kangrui Yuan ◽  
Xiaowei Xing ◽  
...  

In this study, the [Zn(4,4′-bipy)(formic acid)2(NO3)2] (4,4′-bipy = 4,4′-bipyridine) metal-organic framework (MOF) (1) was synthesized using Zn(NO3)2·6H2O and 4,4′-bipyridine according to the solution solvothermal approach. In addition, thermogravimetric analysis (TGA), power X-ray diffraction (XRD), UV spectrophotometry, and Fourier transform infrared spectroscopy (FTIR) were carried out to determine the crystal phase and characterizations of 1. Thereafter, 1 was adopted to carry 5-fluorouracil (5-FU), which displayed a 5-FU loading of 37.9 wt%. Besides, the 5-FU-loaded 1 displayed pH-sensitive behavior with the release of 5-FU in acidic environment. Typically, the 5-FU release process was progressive and under efficient control, and 93% 5-FU was released in acidic environment at 72 h later, but only 58% 5-FU was released in the phosphate buffered saline (PBS, pH = 7.4). Furthermore, the toxicity of 1 was evaluated through cytotoxicity assays in HeLa and L02 cell lines, which showed significantly low toxicity of 1. Taken together, these results indicate that, the prepared 1 may potentially serve as a suitable carrier for drug delivery applications.


Pharmaceutics ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 463 ◽  
Author(s):  
Wu ◽  
Fu ◽  
Zhou ◽  
Wang ◽  
Feng ◽  
...  

Rapid increase of antimicrobial resistance has become an urgent threat to global public health. In this research, since photothermal therapy is a potential antibacterial strategy, which is less likely to cause resistance, a metal–organic framework-based chemo-photothermal combinational system was constructed. Zeolitic imidazolate frameworks-8 (ZIF-8), a porous carrier with unique features such as high loading and pH-sensitive degradation, was synthesized, and then encapsulated photothermal agent indocyanine green (ICG). First, ICG with improved stability in ZIF-8 (ZIF-8-ICG) can effectively produce heat in response to NIR laser irradiation for precise, rapid, and efficient photothermal bacterial ablation. Meanwhile, Zn2+ ions released from ZIF-8 can inhibit bacterial growth by increasing the permeability of bacterial cell membrane and further strengthen photothermal therapy efficacy by reducing the heat resistance of bacteria. Study showed that bacteria suffered from significant changes in morphology after treatment with ZIF-8-ICG under laser irradiation. The combinational chemo-hyperthermia therapy of ZIF-8-ICG could thoroughly ablate murine subcutaneous abscess induced by methicillin-resistant Staphylococcus aureus (MRSA), exhibiting a nearly 100% bactericidal ratio. Both in vitro and in vivo safety evaluation confirmed that ZIF-8-ICG was low toxic. Overall, our researches demonstrated that ZIF-8-ICG has great potential to be served as an alternative to antibiotics in combating multidrug-resistant bacterial pathogens.


2021 ◽  
Vol 22 (24) ◽  
pp. 13622
Author(s):  
Do Nam Lee ◽  
Kihak Gwon ◽  
Yunhee Nam ◽  
Su Jung Lee ◽  
Ngoc Minh Tran ◽  
...  

Polyurethane foams (PUFs) have attracted attention as biomaterials because of their low adhesion to the wound area and suitability as biodegradable or bioactive materials. The composition of the building blocks for PUFs can be controlled with additives, which provide excellent anti-drug resistance and biocompatibility. Herein, nanosized Cu-BTC (copper(II)-benzene-1,3,5-tricarboxylate) was incorporated into a PUF via the crosslinking reaction of castor oil and chitosan with toluene-2,4-diisocyanate, to enhance therapeutic efficiency through the modification of the surface of PUF. The physical and thermal properties of the nanosized Cu-BTC-incorporated PUF (PUF@Cu-BTC), e.g., swelling ratio, phase transition, thermal gravity loss, and cell morphology, were compared with those of the control PUF. The bactericidal activities of PUF@Cu-BTC and control PUF were evaluated against Pseudomonas aeruginosa, Klebsiella pneumoniae, and methicillin-resistant Staphylococcus aureus. PUF@Cu-BTC exhibited selective and significant antibacterial activity toward the tested bacteria and lower cytotoxicity for mouse embryonic fibroblasts compared with the control PUF at a dose of 2 mg mL−1. The Cu(II) ions release test showed that PUF@Cu-BTC was stable in phosphate buffered saline (PBS) for 24 h. The selective bactericidal activity and low cytotoxicity of PUF@Cu-BTC ensure it is a candidate for therapeutic applications for the drug delivery, treatment of skin disease, and wound healing.


2019 ◽  
Vol 11 (47) ◽  
pp. 43989-43995 ◽  
Author(s):  
Xingjun Li ◽  
Shanyong Zhou ◽  
Shan Lu ◽  
Datao Tu ◽  
Wei Zheng ◽  
...  

2019 ◽  
Vol 11 (7) ◽  
pp. 917-923 ◽  
Author(s):  
Neha Bhardwaj ◽  
Sanjeev K. Bhardwaj ◽  
Deepanshu Bhatt ◽  
Satish K. Tuteja ◽  
Ki-Hyun Kim ◽  
...  

In this research, a new luminescent bioprobe was developed for the detection of S. aureus based on bio-conjugation of an amine functionalized metal–organic framework (NH2-MIL-53(Fe)) with an anti-S. aureus antibody (Ab).


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