scholarly journals SERS Biosensor Based on Engineered 2D-Aperiodic Nanostructure for In-Situ Detection of Viable Brucella Bacterium in Complex Matrix

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 886
Author(s):  
Massimo Rippa ◽  
Riccardo Castagna ◽  
Domenico Sagnelli ◽  
Ambra Vestri ◽  
Giorgia Borriello ◽  
...  
Keyword(s):  
High Performance ◽  
Foodborne Pathogen ◽  
Surface Enhanced Raman Spectroscopy ◽  
High Performing ◽  
Sensing Platform ◽  
Food Detection ◽  
Raman Enhancement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
In Situ Detection

Brucella is a foodborne pathogen globally affecting both the economy and healthcare. Surface Enhanced Raman Spectroscopy (SERS) nano-biosensing can be a promising strategy for its detection. We combined high-performance quasi-crystal patterned nanocavities for Raman enhancement with the use of covalently immobilized Tbilisi bacteriophages as high-performing bio-receptors. We coupled our efficient SERS nano-biosensor to a Raman system to develop an on-field phage-based bio-sensing platform capable of monitoring the target bacteria. The developed biosensor allowed us to identify Brucella abortus in milk by our portable SERS device. Upon bacterial capture from samples (104 cells), a signal related to the pathogen recognition was observed, proving the concrete applicability of our system for on-site and in-food detection.

scholarly journals Surface Enhanced Raman Spectroscopy With Electrodeposited Copper Ultramicro-Wires With/Without Silver Nanostars Decoration

Nanomaterials ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 518
Author(s):  
Margherita Longoni ◽  
Maria Sole Zalaffi ◽  
Lavinia de Ferri ◽  
Angela Maria Stortini ◽  
Giulio Pojana ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Enhanced Raman Spectroscopy ◽  
Anodized Aluminum ◽  
Anodized Aluminum Oxide ◽  
Electrochemical Preparation ◽  
Deposition Parameters ◽  
Raman Enhancement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Materials Used

The electrochemical preparation of arrays of copper ultramicrowires (CuUWs) by using porous membranes as templates is critically revisited, with the goal of obtaining cheap but efficient substrates for surface enhanced Raman spectroscopy (SERS). The role of the materials used for the electrodeposition is examined, comparing membranes of anodized aluminum oxide (AAO) vs. track-etched polycarbonate (PC) as well as copper vs. glassy carbon (GC) as electrode material. A voltammetric study performed on bare electrodes and potentiostatic tests on membrane coated electrodes allowed the optimization of the deposition parameters. The final arrays of CuUWs were obtained by chemical etching of the template, with NaOH for AAO and CH2Cl2 for PC. After total etching of the template, SERS spectra were recorded on CuUWs using benzenethiol as SERS probe with known spectral features. The CuUW substrates displayed good SERS properties, providing enhancement factor in the 103–104 range. Finally, it was demonstrated that higher Raman enhancement can be achieved when CuUWs are decorated with silver nanostars, supporting the formation of SERS active hot-spots at the bimetallic interface.

Close-packed assemblies of discrete tiny silver nanoparticles on triangular gold nanoplates as a high performance SERS probe

RSC Advances ◽  
2015 ◽  
Vol 5 (115) ◽  
pp. 94849-94854 ◽  
Author(s):  
Youju Huang ◽  
Palanisamy Kannan ◽  
Lei Zhang ◽  
Yun Rong ◽  
Liwei Dai ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Ag Nanoparticles ◽  
High Performance ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

An island like array of tiny Ag nanoparticles bounded on triangular Au nanoplates was synthesized as surface-enhanced Raman spectroscopy substrate.

Quantification of Protein Biomarker Using SERS Nano-Stress Sensing with Peak Intensity Ratiometry

2016 ◽  
Vol 04 (03) ◽  
pp. 1640011 ◽  
Author(s):  
Douglas Goh ◽  
Kien Voon Kong ◽  
Perumal Jayakumar ◽  
Tianxun Gong ◽  
U.S. Dinish ◽  
...  
Keyword(s):  
Low Cost ◽  
Spectral Characteristics ◽  
Surface Enhanced Raman Spectroscopy ◽  
Biological Molecules ◽  
Peak Intensity Ratio ◽  
Peak Intensity ◽  
Sensing Platform ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Stress Sensing

We report a surface enhanced Raman spectroscopy (SERS) ratiometry method based on peak intensity coupled in a nano-stress sensing platform to detect and quantify biological molecules. Herein, we employed an antibody-conjugated [Formula: see text]-aminothiophenol (ATP) functionalized on a bimetallic-film-over-nanosphere (BMFON) substrate as a sensitive SERS platform to detect human haptoglobin (Hp) protein, which is an acute phase protein and a biomarker for various cancers. Correlation between change in the ATP spectral characteristics and concentration of Hp protein was established by examining the peak intensity ratio at 1572[Formula: see text]cm[Formula: see text] and 1592[Formula: see text]cm[Formula: see text] that reflects the degree of stress experienced by the aromatic ring of ATP during Hp protein–antibody interaction. Development of this platform shows the potential in developing a low-cost and sensitive SERS sensor for the pre-screening of various biomarkers.

High performance gold nanorods and silver nanocubes in surface-enhanced Raman spectroscopy of pesticides

2009 ◽  
Vol 11 (34) ◽  
pp. 7491 ◽  
Author(s):  
Jean Claudio Santos Costa ◽  
Rômulo Augusto Ando ◽  
Antonio Carlos Sant’Ana ◽  
Liane Marcia Rossi ◽  
Paulo Sérgio Santos ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Gold Nanorods ◽  
High Performance ◽  
Surface Enhanced Raman Spectroscopy ◽  
Silver Nanocubes ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

HIGH-ORDERED AND ULTRA-SENSITIVE PARTICLE-IN-BOWL METALLIC ARRAYS FOR SURFACE ENHANCED RAMAN SPECTROSCOPY

NANO ◽  
2014 ◽  
Vol 09 (04) ◽  
pp. 1450050 ◽  
Author(s):  
DI DI ◽  
PEITAO DONG ◽  
CHAOGUANG WANG ◽  
JIAN CHEN ◽  
JUNFENG WANG ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fdtd Method ◽  
Disease Diagnosis ◽  
Surface Enhanced Raman Spectroscopy ◽  
Excitation Wavelength ◽  
Raman Enhancement ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Em Field ◽  
Lift Off

High-ordered particle-in-bowl (PIB) arrays are developed in this paper for surface enhanced Raman spectroscopy (SERS). A heterogeneous shadow mask, composing of the chrome (Cr) layer and colloid residues, is used to fabricate the silicon ( Si ) template from where the PIB arrays finally lift-off. The finite difference time domain (FDTD) method is employed to investigate the Raman enhancement mechanism of this PIB architecture. The electromagnetic (EM) field tends to concentrate in the gap between the bowl and the particle forming the "hot spots". The enhancement factor (EF) of the EM field is about 70 with an excitation wavelength of 785 nm. The Raman measurements validate the EM calculation of the PIB arrays. The EF is about 1.12 × 107 using Rodamine 6G (R6G) as probe molecule. The proposed PIB array is high-ordered in morphology and ultra-sensitive in Raman measurement, providing an ideal substrate for SERS-based bio-chemical sensing, disease diagnosis and analytical chemistry.

scholarly journals Electromagnetic Modelling of Raman Enhancement from Nanoscale Structures as a Means to Predict the Efficacy of SERS Substrates

2007 ◽  
Vol 2007 ◽  
pp. 1-10 ◽  
Author(s):  
Richard J. C. Brown ◽  
Jian Wang ◽  
Martin J. T. Milton
Keyword(s):  
Surface Enhanced Raman Spectroscopy ◽  
Sers Substrate ◽  
Structured Surfaces ◽  
Nanoscale Structures ◽  
Sers Substrates ◽  
Raman Enhancement ◽  
Electromagnetic Modelling ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
First Time

The requirement to optimise the balance between signal enhancement and reproducibility in surface enhanced Raman spectroscopy (SERS) is stimulating the development of novel substrates for enhancing Raman signals. This paper describes the application of finite element electromagnetic modelling to predict the Raman enhancement produced from a variety of SERS substrates with differently sized, spaced and shaped morphologies with nanometre dimensions. For the first time, a theoretical comparison between four major generic types of SERS substrate (including metal nanoparticles, structured surfaces, and sharp tips) has been performed and the results are presented and discussed. The results of the modelling are consistent with published experimental data from similar substrates.

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