scholarly journals Effects on Microbiota Composition after Consumption of Quinoa Beverage Fermented by a Novel Xylose-Metabolizing L. plantarum Strain

Nutrients ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 3318
Author(s):  
Pamela Canaviri-Paz ◽  
Elin Oscarsson ◽  
Anna Kjellström ◽  
Hanna Olsson ◽  
Chandana Jois ◽  
...  
Keyword(s):  
Fragment Length Polymorphism ◽  
Starter Culture ◽  
Probiotic Strain ◽  
Well Being ◽  
Microbiota Composition ◽  
Probiotic Potential ◽  
Faecal Samples ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Restriction Fragment Length

Demands for novel lactic acid bacteria with potential to be used as probiotics along with healthy fermented plant-based products increase worldwide. In this study, a novel Lactiplantibacillus plantarum P31891 strain with enzymatic capacity to degrade tannins and ferment xylose was used as starter culture for fermentation of a quinoa-based beverage. The probiotic potential of the selected strain was evaluated in healthy volunteers. Twenty participants consumed the beverage for 14 days; microbiota changes in saliva and faecal samples were analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP), Next Generation Sequencing (NGS) and qPCR; and gastrointestinal well-being and digestive symptoms were recorded. The results indicated that the consumption of the beverage with Lactiplantibacillus plantarum P31891 in a probiotic dose (1012 CFU/mL) increased the number of Lactobacillus in the feces but not in saliva. Overall, the bacterial community did not seem to be influenced by the bacterium or by the beverage, as expressed by the diversity indexes, but specific genera were affected, as reflected in changes in amplicon sequence variants. Consequently, Lactiplantibacillus plantarum P31891 showed potential to be categorized as a probiotic strain in the fermented quinoa-based beverage.

Analysis of the human intestinal microbiota from 92 volunteers after ingestion of identical meals

2013 ◽  
Vol 4 (2) ◽  
pp. 187-193 ◽  
Author(s):  
J.S. Jin ◽  
M. Touyama ◽  
R. Kibe ◽  
Y. Tanaka ◽  
Y. Benno ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Restriction Fragment Length Polymorphism ◽  
Intestinal Microbiota ◽  
Fragment Length Polymorphism ◽  
Microbiota Composition ◽  
Key Factors ◽  
Enzyme Systems ◽  
Human Intestinal Microbiota ◽  
Faecal Samples ◽  
Restriction Fragment Length

The intestinal microbiota composition of 92 volunteers living in Japan was identified following the consumption of ‘identical meals’ (1,879 kcal/day) for 3 days. When faecal samples were analysed by terminal restriction fragment length polymorphism with several primer-restriction enzyme systems and then clustered, the patterns could be divided into 2 clusters. Contribution tests and partition modelling showed that OTU211 of the 35f-MspI system and OTU237 of the 35f-AluI system were key factors in the distribution of these groups. However, significant differences among these groups in terms of body mass index and age were not observed.

scholarly journals Trends in bacterial and fungal communities in ant nests observed with Terminal-Restriction Fragment Length Polymorphism (T-RFLP) and Next Generation Sequencing (NGS) techniques—validity and compatibility in ecological studies

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5289 ◽  
Author(s):  
Stafva Lindström ◽  
Owen Rowe ◽  
Sari Timonen ◽  
Liselotte Sundström ◽  
Helena Johansson
Keyword(s):  
Next Generation Sequencing ◽  
Restriction Fragment Length Polymorphism ◽  
Microbial Communities ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Illumina Miseq ◽  
Fungal Communities ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing ◽  
Restriction Fragment Length

Microbes are ubiquitous and often occur in functionally and taxonomically complex communities. Unveiling these community dynamics is one of the main challenges of microbial research. Combining a robust, cost effective and widely used method such as Terminal Restriction Fragment Length Polymorphism (T-RFLP) with a Next Generation Sequencing (NGS) method (Illumina MiSeq), offers a solid alternative for comprehensive assessment of microbial communities. Here, these two methods were combined in a study of complex bacterial and fungal communities in the nest mounds of the antFormica exsecta, with the aim to assess the degree to which these methods can be used to complement each other. The results show that these methodologies capture similar spatiotemporal variations, as well as corresponding functional and taxonomical detail, of the microbial communities in a challenging medium consisting of soil, decomposing plant litter and an insect inhabitant. Both methods are suitable for the analysis of complex environmental microbial communities, but when combined, they complement each other well and can provide even more robust results. T-RFLP can be trusted to show similar general community patterns as Illumina MiSeq and remains a good option if resources for NGS methods are lacking.

scholarly journals The Infant-Derived Bifidobacterium bifidum Strain CNCM I-4319 Strengthens Gut Functionality

2020 ◽  
Vol 8 (9) ◽  
pp. 1313
Author(s):  
Rebeca Martín ◽  
Francesca Bottacini ◽  
Muireann Egan ◽  
Celia Chamignon ◽  
Valérie Tondereau ◽  
...  
Keyword(s):  
Probiotic Strain ◽  
Well Being ◽  
Bifidobacterium Bifidum ◽  
Special Role ◽  
Probiotic Potential ◽  
Inflammatory Damage ◽  
Naturally Occurring ◽  
Health And Well Being ◽  
Breast Fed ◽  
Growth Experiments

Bifidobacteria are among the first colonisers of the gastrointestinal tract of breast-fed newborns due to, among other things, their ability to metabolise oligosaccharides naturally occurring in human milk. The presence of bifidobacteria in the infant gut has been shown to promote intestinal health and homeostasis as well as to preserve a functional gut barrier, thus positively influencing host health and well-being. Among human-associated gut commensals, Bifidobacterium bifidum has been described as the only species capable of the extracellular degradation of both mucin-type glycans and HMOs, thereby giving this species a special role as a commensal gut forager of both host and diet-derived glycans. In the present study, we assess the possible beneficial properties and probiotic potential of B. bifidum strain CNCM I-4319. In silico genome analysis and growth experiments confirmed the expected ability of this strain to consume HMOs and mucin. By employing various animal models, we were also able to assess the ability of B. bifidum CNCM I-4319 to preserve gut integrity and functionality from stress-induced and inflammatory damage, thereby enforcing its potential as an effective probiotic strain.

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