scholarly journals Trypanosoma rangeli Genetic, Mammalian Hosts, and Geographical Diversity from Five Brazilian Biomes

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 736
Author(s):  
Maria Augusta Dario ◽  
Márcio Galvão Pavan ◽  
Marina Silva Rodrigues ◽  
Cristiane Varella Lisboa ◽  
Danilo Kluyber ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Mammalian Host ◽  
Trypanosoma Rangeli ◽  
High Genetic Diversity ◽  
Nasua Nasua ◽  
Haplotype Networks ◽  
Blood Clots ◽  
Rdna Amplification

Trypanosoma rangeli is a generalist hemoflagellate that infects mammals and is transmitted by triatomines around Latin America. Due to its high genetic diversity, it can be classified into two to five lineages. In Brazil, its distribution outside the Amazon region is virtually unknown, and knowledge on the ecology of its lineages and on host species diversity requires further investigation. Here, we analyzed 57 T. rangeli samples obtained from hemocultures and blood clots of 1392 mammals captured in different Brazilian biomes. The samples were subjected to small subunit (SSU) rDNA amplification and sequencing to confirm T. rangeli infection. Phylogenetic inferences and haplotype networks were reconstructed to classify T. rangeli lineages and to infer the genetic diversity of the samples. The results obtained in our study highlighted both the mammalian host range and distribution of T. rangeli in Brazil: infection was observed in five new species (Procyon cancrivorous, Priodontes maximum, Alouatta belzebul, Sapajus libidinosus, and Trinomys dimidiatus), and transmission was observed in the Caatinga biome. The coati (Nasua nasua) and capuchin monkey (S. libidinosus) are the key hosts of T. rangeli. We identified all four T. rangeli lineages previously reported in Brazil (A, B, D, and E) and possibly two new genotypes.

Non-monophyly of Retortamonadida and high genetic diversity of the genus Chilomastix suggested by analysis of SSU rDNA

2008 ◽  
Vol 48 (2) ◽  
pp. 770-775 ◽  
Author(s):  
Ivan Cepicka ◽  
Martin Kostka ◽  
Magdalena Uzlíková ◽  
Jaroslav Kulda ◽  
Jaroslav Flegr
Keyword(s):  
Genetic Diversity ◽  
Ssu Rdna ◽  
High Genetic Diversity

Phylogeny, taxonomy and grouping of Trypanosoma rangeli isolates from man, triatomines and sylvatic mammals from widespread geographical origin based on SSU and ITS ribosomal sequences

Parasitology ◽  
2004 ◽  
Vol 129 (5) ◽  
pp. 549-561 ◽  
Author(s):  
F. MAIA DA SILVA ◽  
H. NOYES ◽  
M. CAMPANER ◽  
A. C. V. JUNQUEIRA ◽  
J. R. COURA ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
Geographical Origin ◽  
Small Subunit ◽  
Ribosomal Gene ◽  
Mammalian Host ◽  
Trypanosoma Rangeli ◽  
Wild Mammals ◽  
Triatomine Bugs ◽  
First Time

Phylogenetic relationships among Trypanosoma rangeli isolates from man, wild mammals and triatomine bugs from widespread geographical origin were inferred by comparison of the small subunit of ribosomal gene sequences. The phylogenetic trees indicated that the subgenus Herpetosoma is polyphyletic and strongly supported division of this group into two monophyletic lineages, one made up of T. rangeli, T. rangeli-like and allied species and other consisting of T. lewisi and related taxa. Based on phylogenetic analysis, morphology, behaviour in vertebrate and invertebrate hosts and epidemiology we propose: a) the validation of Herpetosoma as a taxon comprised only for species of group lewisi and the maintenance of T. lewisi as the type-species of this subgenus; b) the classification of T. rangeli, T. rangeli-like and allied species into a ‘T. rangeli-clade’ more closely related to Schizotrypanum than to T. lewisi or T. brucei. The phylogenetic tree disclosed at least 4 groups within the clade T. rangeli, all confirmed by polymorphism of the internal transcribed spacer, thus conferring for the first time phylogenetic support to groups of T. rangeli and corroborating the high complexity of this taxon. Grouping was independent of their mammalian host-species and geographical origin, indicating that other factors are determining this segregation.

scholarly journals Genetic diversity and molecular survey of Trypanosoma (Megatrypanum ) theileri in cattle in Brazil’s western Amazon region

2018 ◽  
Vol 27 (4) ◽  
pp. 579-583 ◽  
Author(s):  
Thábata dos Anjos Pacheco ◽  
Arlei Marcili ◽  
Andrea Pereira da Costa ◽  
Rute Witter ◽  
Andréia Lima Tomé Melo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Molecular Diagnosis ◽  
Prevalence Rate ◽  
Cathepsin L ◽  
Ssu Rdna ◽  
Amazon Region ◽  
Prevalence Rates ◽  
High Genetic Diversity ◽  
First Time

Abstract Trypanosoma (Megatrypanum) theileri is a flagellated protozoan that infects ruminants and it displays high genetic diversity. In this study, we investigated the prevalence rates of this protozoan based on hemoculture and molecular diagnosis. The isolates of T. theileri thus obtained were characterized by molecular markers SSU rDNA and gGAPDH and molecular diagnosis based on Cathepsin L-like gene (PCR-TthCATL). The PCR-TthCATL and hemoculture indicated an overall prevalence rate of 8.13%, and the CATL derived sequence named IB was identified for the first time in cattle in the western Amazon region, as well as IF in Brazil. We also describe a possible new PCR-TthCATL derived sequence in cattle, designated IL.

scholarly journals Genetic diversity analysis of an isolate of microsporidia infesting Athetis lepigone

2019 ◽  
Author(s):  
Haijian Zhang ◽  
Jian Song ◽  
Yunhe Yang ◽  
Jingjie An ◽  
Hongxia Ma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Sequencing Analysis ◽  
Multiple Sequence ◽  
Intergenic Spacers ◽  
Link Type ◽  
Close Relationship ◽  
Small Subunit Ribosomal Dna

AbstractIn this study, PCR amplification, cloning and sequencing analysis were adopted to explore genetic diversity of microsporidia (LEP9557) infecting Athetis lepigone. The small subunit ribosomal DNA (SSU rDNA), internal transcribed spacer (ITS) and intergenic spacers (IGS) of ribosomal RNA (rRNA) were cloned from the strain and sequenced. By means of multiple sequence alignment, we found that the three gene regions had different levels of polymorphism. There was greater polymorphism in ITS (74 variable sites) and IGS (55.59%) regions than in the SSU rDNA (17 variable sites). Phylogenetic analysis was performed using Kimura 2-parameter with neighbor joining and the results showed that LEP9557 had a close relationship with Nosema bombycis. Sequences of each clone were submitted to Genbank (Accession number: MF150254-MF150258). All of the results indicated the presence of genetic diversity in LEP9557, which established the foundation for identifying the phylogeny and relationships with other microsporidian strains, and had significant biological meaning for maintaining the survival and population continuity of the strain.

scholarly journals Genetic Diversity of Cryptosporidium parvum in Neonatal Dairy Calves in Xinjiang, China

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 692
Author(s):  
Yayun Wu ◽  
Kuankuan Zhang ◽  
Ying Zhang ◽  
Bo Jing ◽  
Yuancai Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Cryptosporidium Parvum ◽  
Diarrheal Disease ◽  
Small Subunit ◽  
Dairy Calves ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
Small Subunit Rrna ◽  
High Genetic Diversity ◽  
Glycoprotein Gene

Cryptosporidium parvum has been identified as a major cause of diarrhea and diarrhea-associated deaths in young children and neonatal calves. Infections can remain asymptomatic but may lead to malnutrition and persistent growth retardation. To assess the relationship between C. parvum genetic diversity and pathogenicity in neonatal dairy calves and determine the cause of diarrhea among these calves, 232 fecal samples from neonatal dairy calves on 12 farms in Xinjiang, China, were characterized for Cryptosporidium presence based on the small subunit rRNA gene. The Cryptosporidium prevalence was 38.4% (89/232), and three species were detected with restriction fragment length polymorphism analysis, including C. parvum (the significantly dominant species), C. ryanae, and C. bovis. Cryptosporidium prevalence was significantly higher in neonatal dairy calves with diarrhea (52.6%, 51/97) than in calves without diarrhea (28.1%, 38/135). All C. parvum-positive samples were analyzed based on the 60 KDa glycoprotein gene, and IIdA15G1, IIdA20G1, IIdA14G1, and IIdA19G1 were successfully subtyped. These data indicate that C. parvum may be a major contributor to diarrheal disease in neonatal dairy calves, and C. parvum subtypes from neonatal dairy calves in Xinjiang exhibited high genetic diversity.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

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