scholarly journals Molecular Typing of Neisseria gonorrhoeae Clinical Isolates in Russia, 2018–2019: A Link Between penA Alleles and NG-MAST Types

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 941
Author(s):  
Ilya Kandinov ◽  
Ekaterina Dementieva ◽  
Dmitry Kravtsov ◽  
Alexander Chestkov ◽  
Alexey Kubanov ◽  
...  
Keyword(s):  
Regression Analysis ◽  
Neisseria Gonorrhoeae ◽  
Molecular Typing ◽  
Promoter Region ◽  
Gene Polymorphisms ◽  
Genetic Factors ◽  
Clinical Isolates ◽  
Minimum Inhibitory Concentrations ◽  
Allele Type

This work aimed to study penA gene polymorphisms in clinical isolates of Neisseria gonorrhoeae collected in Russia in 2018–2019 and the contribution of the penA allele type to susceptibility to β-lactam antibiotics. A total of 182 isolates were analyzed. penA allele types were determined by sequencing, and the minimum inhibitory concentrations (MICs) of benzylpenicillin and ceftriaxone were measured. The influence of genetic factors on MICs was evaluated by regression analysis. All isolates were susceptible to ceftriaxone, and 40.1% of isolates were susceptible to penicillin. Eleven penA allele types were identified. The mosaic type XXXIV penA allele and the Gly120Lys substitution in PorB made the greatest contributions to increasing the ceftriaxone MIC; the presence of the blaTEM plasmid, Gly120Asp, Ala121Gly/Asn substitutions in PorB, and the adenine deletion in the promoter region of the mtrR gene caused an increase in the penicillin MIC. Among 61 NG-MAST types identified, the most frequent were types 228, 807, 9486, 1993, and 6226. A link between penA alleles and Neisseria gonorrhoeae multi-antigen sequence typing (NG-MAST) types was established. Resistance to two groups of β-lactam antibiotics was associated with non-identical changes in penA alleles. To prevent the emergence of ceftriaxone resistance in Russia, NG-MAST genotyping must be supplemented with penA allele analysis.

scholarly journals Molecular characterization of a ceftriaxone-resistant Neisseria gonorrhoeae strain found in Switzerland: a case report

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Konrad Egli ◽  
Anna Roditscheff ◽  
Ursula Flückiger ◽  
Martin Risch ◽  
Lorenz Risch ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
23S Rrna ◽  
Rrna Gene ◽  
Limited Information ◽  
Specific Pcr ◽  
Appropriate Treatment ◽  
23S Rrna Gene ◽  
Allele Type ◽  
Ciprofloxacin Resistance

Abstract Background The resistance of Neisseria gonorrhoeae to ceftriaxone is unusual in Switzerland. The underlying genotype responsible for resistance is suspected to be novel. Generally, resistance in Neisseria gonorrhoeae (Ng) involves a comprehensive set of genes with many different mutations leading to resistance to different β-lactams and fluoroquinolones. Case presentation A patient had a positive result from specific PCR for Ng. We routinely culture all clinical specimens with a positive NG-PCR. In this particular case, we isolated a strain with resistance to ceftriaxone in Switzerland. A total of seven different genes (penA, ponA, porinB, mtr, gyrA, parC, 23S rRNA gene) in this strain were partially sequenced for comparison with phenotypic susceptibility testing. Interestingly, two different mutations in the porinB gene were observed, and data on this gene are limited. Information on the identified allele type of the penA gene is very limited as well. Three different mutations of parC and gyrA that correlate with ciprofloxacin resistance were found. The combination of ceftriaxone and ciprofloxacin resistance makes an appropriate treatment difficult to obtain due to multidrug resistance. Conclusion The combined results for all genes show the appearance of new mutations in central Europe either due to worldwide spread or the emergence of new genetic combinations of mutations.

scholarly journals Resistance to penicillin and identification of penicillinase-producing Neisseria gonorrhoeae among clinical isolates in Thailand.

1980 ◽  
Vol 18 (2) ◽  
pp. 360-361 ◽  
Author(s):  
J W Crum ◽  
C Duangmani ◽  
S Vibulyasekha ◽  
K Suthisomboon
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Clinical Isolates ◽  
Resistance To Penicillin

scholarly journals Molecular Typing of Neisseria gonorrhoeae Isolates by Pyrosequencing of Highly Polymorphic Segments of the porB Gene

2004 ◽  
Vol 42 (7) ◽  
pp. 2926-2934 ◽  
Author(s):  
M. Unemo ◽  
P. Olcen ◽  
J. Jonasson ◽  
H. Fredlund
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Molecular Typing

scholarly journals Chromosome-Encoded Class D β-Lactamase OXA-23 in Proteus mirabilis

2002 ◽  
Vol 46 (6) ◽  
pp. 2004-2006 ◽  
Author(s):  
R. Bonnet ◽  
H. Marchandin ◽  
C. Chanal ◽  
D. Sirot ◽  
R. Labia ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Proteus Mirabilis ◽  
Molecular Typing ◽  
Clinical Isolates ◽  
Clonal Strain ◽  
Class D ◽  
Chromosome Fragments

ABSTRACT Ten nonrepetitive Proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of Clermont-Ferrand, France, produced class D carbapenemase OXA-23. MICs of imipenem were 0.25 to 0.5 μg/ml for these clinical isolates. Molecular typing revealed that the 10 P. mirabilis isolates originated from the same clonal strain. Hybridization of I-CeuI-generated chromosome fragments with a bla OXA-23 probe showed that the gene was chromosome encoded in the P. mirabilis strain.

scholarly journals Spectinomycin: minimum inhibitory concentrations for Neisseria gonorrhoeae.

1974 ◽  
Vol 50 (4) ◽  
pp. 289-290
Author(s):  
I A Porter ◽  
W J Wood
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Minimum Inhibitory Concentrations

scholarly journals Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene (penA) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime

2002 ◽  
Vol 46 (12) ◽  
pp. 3744-3749 ◽  
Author(s):  
Satoshi Ameyama ◽  
Shoichi Onodera ◽  
Masahiro Takahata ◽  
Shinzaburo Minami ◽  
Nobuko Maki ◽  
...  
Keyword(s):  
Streptococcus Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Neisseria Meningitidis ◽  
Binding Protein ◽  
Clinical Isolates ◽  
Penicillin Binding Protein ◽  
Gene Sequences ◽  
Neisseria Flavescens ◽  
Neisseria Perflava

ABSTRACT Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene (penA) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava (N. sicca), Neisseria cinerea, Neisseria flavescens, and Neisseria meningitidis. These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae.

Resistance, molecular characterization and viability of Neisseria gonorrhoeae recent clinical isolates

2021 ◽  
Vol 156 (5) ◽  
pp. 249-250
Author(s):  
Elizabeth Calatrava-Hernández ◽  
Carla Foronda-García-Hidalgo ◽  
José Gutiérrez-Fernández
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Molecular Characterization ◽  
Clinical Isolates

The relationship between hyperbilirubinemia and the promoter region and first exon of UGT1A1 gene polymorphisms in Vietnamese newborns

2020 ◽  
Vol 88 (6) ◽  
pp. 940-944
Author(s):  
Tien-Thanh Nguyen ◽  
Wei Zhao ◽  
Xi Yang ◽  
Dan-Ni Zhong
Keyword(s):  
Promoter Region ◽  
Gene Polymorphisms ◽  
Ugt1a1 Gene ◽  
The Relationship
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