scholarly journals Chromosome-Encoded Class D β-Lactamase OXA-23 in Proteus mirabilis

2002 ◽  
Vol 46 (6) ◽  
pp. 2004-2006 ◽  
Author(s):  
R. Bonnet ◽  
H. Marchandin ◽  
C. Chanal ◽  
D. Sirot ◽  
R. Labia ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Proteus Mirabilis ◽  
Molecular Typing ◽  
Clinical Isolates ◽  
Clonal Strain ◽  
Class D ◽  
Chromosome Fragments

ABSTRACT Ten nonrepetitive Proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of Clermont-Ferrand, France, produced class D carbapenemase OXA-23. MICs of imipenem were 0.25 to 0.5 μg/ml for these clinical isolates. Molecular typing revealed that the 10 P. mirabilis isolates originated from the same clonal strain. Hybridization of I-CeuI-generated chromosome fragments with a bla OXA-23 probe showed that the gene was chromosome encoded in the P. mirabilis strain.

scholarly journals In-vitro activities of cefepime and other beta-lactam antibiotics against clinical isolates from a Colombian teaching hospital

1998 ◽  
Vol 42 (4) ◽  
pp. 550-552 ◽  
Author(s):  
S. Mattar ◽  
L. Sanchez ◽  
D. Perez ◽  
A. Arango ◽  
R. Parodi ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Clinical Isolates ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics

scholarly journals Comparison of PCR-based molecular markers for the characterization of Proteus mirabilis clinical isolates

2008 ◽  
Vol 12 (5) ◽  
Author(s):  
Lessandra Michelim ◽  
Gabriela Muller ◽  
Jucimar Zacaria ◽  
Ana Paula Longaray Delamare ◽  
Sérgio Olavo Pinto da Costa ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Proteus Mirabilis ◽  
Clinical Isolates

Molecular typing and in vitro resistance of Cryptococcus neoformans clinical isolates obtained in Germany between 2011 and 2017

2019 ◽  
Vol 309 (6) ◽  
pp. 151336 ◽  
Author(s):  
Regina Selb ◽  
Vidmante Fuchs ◽  
Barbara Graf ◽  
Axel Hamprecht ◽  
Michael Hogardt ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Molecular Typing ◽  
Clinical Isolates

scholarly journals Diversity of TEM Mutants in Proteus mirabilis

1999 ◽  
Vol 43 (11) ◽  
pp. 2671-2677 ◽  
Author(s):  
R. Bonnet ◽  
C. De Champs ◽  
D. Sirot ◽  
C. Chanal ◽  
R. Labia ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Substitution ◽  
Proteus Mirabilis ◽  
Clinical Isolates ◽  
Amino Acid Substitutions ◽  
First Report ◽  
Conjugative Plasmids ◽  
Extended Spectrum ◽  
Nucleotide Mutation

ABSTRACT In a survey of resistance to amoxicillin among clinical isolates ofProteus mirabilis, 10 TEM-type β-lactamases were characterized: (i) the well-known penicillinases TEM-1 and TEM-2, the extended-spectrum β-lactamases (ESBLs) TEM-3 and TEM-24, and the inhibitor-resistant TEM (IRT) TEM-44 and (ii) five novel enzymes, a penicillinase TEM-57 similar to TEM-1, an ESBL TEM-66 similar to TEM-3, and three IRTs, TEM-65, TEM-73, and TEM-74. The penicillinase TEM-57 and the ESBL TEM-66 differed from TEM-1 and TEM-3, respectively, by the amino acid substitution Gly-92→Asp (nucleotide mutation G-477→A). This substitution could have accounted for the decrease in pIs (5.2 for TEM-57 and 6.0 for TEM-66) but did not necessarily affect the intrinsic activities of these enzymes. The IRT TEM-65 was an IRT-1-like IRT (Cys-244) related to TEM-2 (Lys-39). The two other IRTs, TEM-73 and TEM-74, were related to IRT-1 (Cys-244) and IRT-2 (Ser-244), respectively, and harbored the amino acid substitutions Leu-21→Phe and Thr-265→Met. In this study, the ESBLs TEM-66, TEM-24, and TEM-3 were encoded by large (170- to 180-kb) conjugative plasmids that exhibited similar patterns after digestion and hybridization with the TEM and AAC(6′)I probes. The three IRTs TEM-65, TEM-73, and TEM-74 were encoded by plasmids that ranged in size from 42 to 70 kb but for which no transfer was obtained. The characterization of five new plasmid-mediated TEM-type β-lactamases and the first report of TEM-24 in P. mirabilis are evidence of the wide diversity of β-lactamases produced in this species and of its possible role as a β-lactamase-encoding plasmid reservoir.

Molecular typing, in vitro susceptibility and virulence of Cryptococcus neoformans/Cryptococcus gattii species complex clinical isolates from south‐eastern Brazil

Mycoses ◽  
2020 ◽  
Vol 63 (12) ◽  
pp. 1341-1351
Author(s):  
Patrícia Helena Grizante Barião ◽  
Ludmilla Tonani ◽  
Tiago Alexandre Cocio ◽  
Roberto Martinez ◽  
Érika Nascimento ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Molecular Typing ◽  
Species Complex ◽  
Cryptococcus Gattii ◽  
Clinical Isolates ◽  
In Vitro Susceptibility ◽  
South Eastern ◽  
Eastern Brazil
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