The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a Streptococcus mutans Cultivated Environment

The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a Streptococcus mutans cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against Streptococcus mutans was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of S. mutans, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.

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Effects of Poly(Amidoamine) Dendrimer-Coated Mesoporous Bioactive Glass Nanoparticles on Dentin Remineralization

Nanomaterials ◽

10.3390/nano9040591 ◽

2019 ◽

Vol 9 (4) ◽

pp. 591 ◽

Cited By ~ 3

Author(s):

Jungin Bae ◽

Woo-Sung Son ◽

Kyung-Hyeon Yoo ◽

Seog-Young Yoon ◽

Moon-Kyoung Bae ◽

...

Keyword(s):

Bioactive Glass ◽

Calcium Ion ◽

Pamam Dendrimer ◽

Dentin Hypersensitivity ◽

Dentinal Tubule ◽

Dentinal Tubules ◽

Releasing Effect ◽

Clinical Conditions ◽

Tubule Occlusion ◽

Mesoporous Bioactive Glass

Dentin hypersensitivity (DH) is one of the most common clinical conditions usually associated with exposed dentinal surfaces. In this study, we identified the effectiveness of poly(amidoamine) (PAMAM) dendrimer-coated mesoporous bioactive glass nanoparticles (MBN) (PAMAM@MBN) on DH treatment, examining the ion-releasing effect, dentin remineralization, and the occluding effect of dentinal tubules. We synthesized MBN and PAMAM@MBN. After soaking each sample in simulated body fluid (SBF), we observed ion-releasing effects and dentin remineralization effects for 30 days. Also, we prepared 30 premolars to find the ratio of occluded dentinal tubules after applying MBN and PAMAM@MBN, respectively. The results showed that PAMAM did not disrupt the calcium ion-releasing ability or the dentin remineralization of MBN. The PAMAM@MBN showed a better occluding effect for dentinal tubules than that of MBN (p < 0.05). In terms of dentinal tubule occlusion, the gap between MBN was well occluded due to PAMAM. This implies that PAMAM@MBN could be effectively used in dentinal tubule sealing and remineralization.

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Mesoporous Bioactive Glass Combined with Graphene Oxide Quantum Dot as a New Material for a New Treatment Option for Dentin Hypersensitivity

Nanomaterials ◽

10.3390/nano10040621 ◽

2020 ◽

Vol 10 (4) ◽

pp. 621

Author(s):

Sung-Ae Son ◽

Dong-Hyun Kim ◽

Kyung-Hyeon Yoo ◽

Seog-Young Yoon ◽

Yong-Il Kim

Keyword(s):

Graphene Oxide ◽

Electron Microscope ◽

Quantum Dot ◽

Bioactive Glass ◽

Pore Size ◽

Field Emission ◽

Dentin Hypersensitivity ◽

Dentinal Tubules ◽

New Material ◽

Mesoporous Bioactive Glass

Dentin hypersensitivity is one of the most common clinical conditions usually associated with exposed dentinal tubules. The purpose of this study was to identify the potential of a graphene oxide quantum dot coating for mesoporous bioactive glass nanoparticles as a new material for the treatment of dentin hypersensitivity by investigating its mineralization activity and dentinal tubules sealing. Mesoporous bioactive glass nanoparticle was fabricated by modified sol-gel synthesis. X-ray diffraction was performed to characterize the synthesized nanoparticle Fourier transform infra-red spectroscopy investigated the functionalized surfaces. The distribution of the specific surface area and the pore size was measure by Pore size analysis. The morphology of sample was observed by Field Emission Scanning Electron Microscope (FESEM) and Field Emission Transmission Electron Microscope (FETEM). After disk-shaped specimens of mesoporous bioactive glass nanoparticles and graphene oxide quantum dot coated mesoporous bioactive glass nanoparticles (n = 3) were soaked in the simulated body fluid for 0, 1, 5, 10,and 30 days, the amount of ions released was observed to confirm the ionic elution for mineralization. Sensitive tooth model discs (n = 20) were applied with two samples and evaluated the dentinal tubule sealing ability. The spherical mesoporous bioactive glass nanoparticles and graphene oxide quantum dot coated mesoporous bioactive glass nanoparticles with a diameter of about 500 nm were identified through FESEM and FETEM. The ion release capacity of both samples appeared to be very similar. The amount of ion released and in vitro mineralization tests confirmed that graphene oxide quantum dot coating of mesoporous bioactive glass nanoparticles did not inhibit the release of calcium, silicon and phosphate ions, but rather that graphene oxide quantum dot promoted hydroxyapatite formation. In the FESEM image of the sensitive tooth disc surface, it was observed that graphene oxide quantum dot coated mesoporous bioactive glass nanoparticles sealed tightly the dentinal tubules. The graphene oxide quantum dot coating of mesoporous bioactive glass nanoparticles not only showed the excellent dentinal sealing ability but also rapidly promoted mineralization while minimizing the size increase by coating the mesoporous bioactive glass nanoparticles.

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Application of Fluorine Containing Bioactive Glass Nanoparticles in Dentin Hypersensitivity Treatment

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.696.103 ◽

2016 ◽

Vol 696 ◽

pp. 103-107 ◽

Cited By ~ 4

Author(s):

Carolina Emmanuelle Camargos Lins ◽

Sandhra Maria de Carvalho ◽

Agda Aline Rocha de Oliveira ◽

Marivalda de Magalhães Pereira

Keyword(s):

Heat Treatment ◽

Bioactive Glass ◽

Glass Structure ◽

Spherical Shape ◽

Dentin Hypersensitivity ◽

Dentinal Tubules ◽

Mineralized Tissues ◽

The Matrix ◽

New System

The effect of Bioactive Glass on remineralization of dentin is the focus of the present study due to its excellent regenerative properties in mineralized tissues. It is known that the effect of Bioactive Glass can be enhanced at the nanoscale. In addition, the incorporation of Fluorine in the glass structure makes possible the formation of Fluorapatite (FAP). The aim of this study was to synthesize and characterize a new system of Fluorine containing Bioactive Glass Nanoparticles (FBGNP), and evaluate the potential for in vitro dentin remineralization by occlusion of dentinal tubules. The FBGNPs produced were uniform, with spherical shape and nanoscale size. Agglomeration or partial sinterization of the particulate system probably occurred after heat treatment. The results suggest the formation of FAP crystals embedded within the matrix of the Bioactive Glass. The gel containing FBGNP produced was effective in obliterating the dentinal tubules in vitro, showing that FBGNP is a potential material to be used for treatment of dentin hypersensitivity.

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Preparation and characterization of silver nanocrystals decorated mesoporous bioactive glass via synchrotron X-ray reduction

Journal of Non-Crystalline Solids ◽

10.1016/j.jnoncrysol.2016.08.012 ◽

2016 ◽

Vol 450 ◽

pp. 128-134 ◽

Cited By ~ 4

Author(s):

Fang-Yu Fan ◽

May-Show Chen ◽

Chia-Wei Wang ◽

Shao-Ju Shih ◽

Chin-Yi Chen ◽

...

Keyword(s):

Bioactive Glass ◽

X Ray ◽

Mesoporous Bioactive Glass

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Evaluation of the Effects of 810 nm Diode Laser Alone and in Combination With Gluma© and Chromophore on Dentinal Tubule Occlusion: A Scanning Electron Microscopic Analysis

Journal of lasers in medical sciences ◽

10.34172/jlms.2020.45 ◽

2020 ◽

Vol 11 (3) ◽

pp. 268-273

Author(s):

Zahra Khoubrouypak ◽

Masoumeh Hasani Tabatabaei ◽

Nasim Chiniforush ◽

Zohreh Moradi

Keyword(s):

Laser Irradiation ◽

Diode Laser ◽

Smear Layer ◽

Dentin Hypersensitivity ◽

Pairwise Comparisons ◽

Dentinal Tubule ◽

Dentinal Tubules ◽

Layer Removal ◽

Irradiation Energy ◽

Scanning Electron

Introduction: Tooth hypersensitivity is among the most common patient complaints caused by the response of exposed dentin to external stimuli. No definite treatment has been suggested so far for dentin hypersensitivity (DH). This study aimed to compare the efficacy of the diode laser alone and in combination with Gluma and chromophore in occluding opened dentine tubules and the treatment of DH. Methods: This in vitro study was conducted on 30 extracted human third molars kept in 0.1% thymol solution. The teeth were mounted in transparent acrylic resin and the buccal enamel was cut to expose the outer third of dentin. The samples were then divided into 6 groups of negative control (no smear layer removal), positive control (smear layer removal with 6% citric acid application), 810 nm diode laser irradiation (energy density 55.55 J/cm2 , 1 W for 20 seconds in a continuous mode), chromophore (1 mg/mL indocyanine green, ICG) plus diode laser irradiation (1 W, 20 seconds), Gluma plus diode laser irradiation, and Gluma + chromophore + diode laser. Dentinal tubules were evaluated under a scanning electron microscope at x2000 magnification. The mean percentage of the obstruction of dentinal tubules was reported as mean and standard deviation. Considering the normal distribution of the data, two-way ANOVA was applied to compare the efficacy of treatments, and an independent-samples t test was used for pairwise comparisons at P<0.05 level of significance. Results: The highest mean percentage of the obstruction of dentinal tubules was noted in the diode laser/chromophore/Gluma group (65.68±12.31%) while the lowest value was noted in the diode laser/Gluma group (24.33±5.90%). Pairwise comparisons revealed significant differences between all groups (P<0.05) except for the difference between the laser/Gluma/chromophore and laser/ chromophore groups (P=0.20). Conclusion: It seems that chromophore increases the efficacy of the diode laser for the obstruction of dentinal tubules.

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Effectiveness of Thymoquinone and Fluoridated Bioactive Glass/Nano-Oxide Contained Dentifrices on Abrasion and Dentine Tubules Occlusion: An Ex Vivo Study

European Journal of Dentistry ◽

10.1055/s-0040-1703418 ◽

2020 ◽

Vol 14 (01) ◽

pp. 045-054 ◽

Cited By ~ 1

Author(s):

Daud Anthoney ◽

Shahreen Zahid ◽

Hina Khalid ◽

Zohaib Khurshid ◽

Asma Tufail Shah ◽

...

Keyword(s):

Bioactive Glass ◽

Ex Vivo ◽

Three Dimensional ◽

International Standards ◽

P Value ◽

Dentin Hypersensitivity ◽

Dentinal Tubules ◽

Tooth Structure ◽

Nano Zinc Oxide ◽

Nano Zinc

Abstract Objectives Dentin hypersensitivity (DH) is mainly due to the loss and replenishment of minerals from tooth structure, where the lost minerals can be rehabilitated with a biomimetic approach. The objectives were to determine the relative dentin abrasivity (RDA) of experimental (EXT) dentifrices and to determine the efficacy to occlude dentinal tubules. Materials and Methods Experimental dentifrices contained nano-fluoridated bioactive glass (n-FBG: 1.5 wt.% [EXT-A], 2.5 wt.% [EXT-B], and 3.5 wt.% [EXT-C]), nano-zinc oxide (n-ZnO), and thymoquinone as active agents. Bovine dentin blocks were subjected to brushing treatments as per groups, that is, distilled water; commercial dentifrice (control, CT); EXT toothpastes; and EXT-D without active agents. Samples were tested for three-dimensional (3D) abrasion analysis according to ISO-11609:2010 (International Organization for Standardization [ISO]). The roughness average (Ra), RDA, surface topography, and elemental compositions were investigated. Statistical Analysis One-way analysis of variance (ANOVA) with post-hoc Tukey’s and Tamhane’s test was performed for characterizations using Statistical Package for the Social Sciences (SPSS) version 21. The result was considered significant with p-value ≤ 0.05. Results Comparisons of Ra differed significantly between all groups with p < 0.05 except CT and EXT-A. The RDA values of EXT-A, EXT-B, and EXT-C were calculated as 74.04, 84.26, and 116.24, respectively, which were well within the acceptable limit set by international standards. All n-FBG containing dentifrices demonstrated uniform occlusion of dentinal tubules; however, highly concentrated EXT dentifrices showed more occlusion. Conclusions Acceptable range of RDA and superior occlusion of tubules by novel dentifrices suggest that it may be recommended for treating DH.

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An Experiment Of Using X-Ray Microtomography (XMT) To Study The Depth Of Bonding Agent Penetration Into Dentinal Tubules

IIUM Medical Journal Malaysia ◽

10.31436/imjm.v16i2.1046 ◽

2017 ◽

Vol 16 (2) ◽

Author(s):

Norzaiti Mohd Kenali

Keyword(s):

Penetration Depth ◽

Gamma Rays ◽

Bonding Agent ◽

Linear Attenuation Coefficient ◽

X Rays ◽

Depth Of Penetration ◽

Bonding Agents ◽

Dentinal Tubules ◽

X Ray ◽

Molar Teeth

Introduction: This is a novel study of the depth of penetration of bonding agents (BA) by using a miniaturised CT-scan, XMT.The Linear Attenuation Coefficient describes the fraction of a beam of x-rays or gamma rays that is absorbed or scattered per unit thickness of the absorber. The higher the LAC, the more opaque the image is. Materials and Methods: The primer and sealer of bonding agent (BA) were added with contrast, iodine and tin methacrylate respectively. Each drop of the mixture were scanned for 1 hour with Mu Cat2 XMT. Linear Attenuation co-efficient (LAC) of the BA must be optimized to appear more than the enamel & dentine. 3 extracted molar teeth were sectioned up to the dentine layer and the dentine were acid etched, dried and applied with the BA. The teeth were scanned by using the same machine for 24 hours and the penetration depth of the BA was studied by using in house (Queen Mary University of London) software. Results: The LAC of dentine was 1.6 cm-1, enamel 2.6 cm-1 and the primer was 3.2 cm-1. Penetration depth of BA was about 30-300um. However, the penetration depth of the BA were not homogenous throughout the dentine. Conclusion(s): We have demonstrated that XMT gives a clear picture of penetration depth of the contrast added primer with iodine that warrants further studies in this field.

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