Application of Fluorine Containing Bioactive Glass Nanoparticles in Dentin Hypersensitivity Treatment

2016 ◽  
Vol 696 ◽  
pp. 103-107 ◽  
Author(s):  
Carolina Emmanuelle Camargos Lins ◽  
Sandhra Maria de Carvalho ◽  
Agda Aline Rocha de Oliveira ◽  
Marivalda de Magalhães Pereira
Keyword(s):  
Heat Treatment ◽  
Bioactive Glass ◽  
Glass Structure ◽  
Spherical Shape ◽  
Dentin Hypersensitivity ◽  
Dentinal Tubules ◽  
Mineralized Tissues ◽  
The Matrix ◽  
New System

The effect of Bioactive Glass on remineralization of dentin is the focus of the present study due to its excellent regenerative properties in mineralized tissues. It is known that the effect of Bioactive Glass can be enhanced at the nanoscale. In addition, the incorporation of Fluorine in the glass structure makes possible the formation of Fluorapatite (FAP). The aim of this study was to synthesize and characterize a new system of Fluorine containing Bioactive Glass Nanoparticles (FBGNP), and evaluate the potential for in vitro dentin remineralization by occlusion of dentinal tubules. The FBGNPs produced were uniform, with spherical shape and nanoscale size. Agglomeration or partial sinterization of the particulate system probably occurred after heat treatment. The results suggest the formation of FAP crystals embedded within the matrix of the Bioactive Glass. The gel containing FBGNP produced was effective in obliterating the dentinal tubules in vitro, showing that FBGNP is a potential material to be used for treatment of dentin hypersensitivity.

scholarly journals Effects of Poly(Amidoamine) Dendrimer-Coated Mesoporous Bioactive Glass Nanoparticles on Dentin Remineralization

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 591 ◽  
Author(s):  
Jungin Bae ◽  
Woo-Sung Son ◽  
Kyung-Hyeon Yoo ◽  
Seog-Young Yoon ◽  
Moon-Kyoung Bae ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Calcium Ion ◽  
Pamam Dendrimer ◽  
Dentin Hypersensitivity ◽  
Dentinal Tubule ◽  
Dentinal Tubules ◽  
Releasing Effect ◽  
Clinical Conditions ◽  
Tubule Occlusion ◽  
Mesoporous Bioactive Glass

Dentin hypersensitivity (DH) is one of the most common clinical conditions usually associated with exposed dentinal surfaces. In this study, we identified the effectiveness of poly(amidoamine) (PAMAM) dendrimer-coated mesoporous bioactive glass nanoparticles (MBN) (PAMAM@MBN) on DH treatment, examining the ion-releasing effect, dentin remineralization, and the occluding effect of dentinal tubules. We synthesized MBN and PAMAM@MBN. After soaking each sample in simulated body fluid (SBF), we observed ion-releasing effects and dentin remineralization effects for 30 days. Also, we prepared 30 premolars to find the ratio of occluded dentinal tubules after applying MBN and PAMAM@MBN, respectively. The results showed that PAMAM did not disrupt the calcium ion-releasing ability or the dentin remineralization of MBN. The PAMAM@MBN showed a better occluding effect for dentinal tubules than that of MBN (p < 0.05). In terms of dentinal tubule occlusion, the gap between MBN was well occluded due to PAMAM. This implies that PAMAM@MBN could be effectively used in dentinal tubule sealing and remineralization.

scholarly journals The Antibacterial and Remineralization Effect of Silver-Containing Mesoporous Bioactive Glass Sealing and Er-YAG Laser on Dentinal Tubules Treated in a Streptococcus mutans Cultivated Environment

2021 ◽  
Vol 14 (11) ◽  
pp. 1124
Author(s):  
Jung-Chang Kung ◽  
Wei-Hsun Wang ◽  
Yu-Ching Chiang ◽  
Yuan-Ting Yang-Wang ◽  
Yueh-Ching Wang ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Laser Irradiation ◽  
Penetration Depth ◽  
Streptococcus Mutans ◽  
Er:Yag Laser ◽  
Dentin Hypersensitivity ◽  
Simultaneous Application ◽  
Dentinal Tubules ◽  
X Ray ◽  
Mesoporous Bioactive Glass

The aim of this study was to evaluate the remineralization and antibacterial effect of silver-containing mesoporous bioactive glass (MBG-Ag) sealing combined with Er:YAG laser irradiation on human demineralized dentin specimens in a Streptococcus mutans cultivated environment. A total of 48 human dentin specimens were randomly divided into four groups. The characteristics of MBG-Ag and the occlusion efficiency of the dentinal tubules were analyzed using X-ray diffraction patterns, Fourier-transform infrared spectroscopy, scanning electron microscope images and energy dispersive X-ray spectroscopy. Moreover, the antibacterial activity against Streptococcus mutans was evaluated by colony formation assay. The results showed that the dentin specimens with Er:YAG laser irradiation can form a melted occlusion with a size of 3–4 µm. MBG-Ag promoted the deposition of numerous crystal particles on the dentinal surface, reaching the deepest penetration depth of 70 μm. The results suggested that both MBG-Ag and laser have the ability to enhance the remineralization and precipitation of hydroxyapatite crystals. While the results showed that MBG-Ag sealing combined with the thermomechanical subablation mode of Er:YAG laser irradiation-induced dense crystalline deposition, reaching a penetration depth of more than 300 µm, silver nanoparticles without good absorption of the Er:YAG laser resulted in a heterogeneous radiated surface. Er:YAG laser irradiation with a low energy and pulse rate cannot completely inhibit the growth of S. mutans, but MBG-Ag sealing reached the bactericidal concentration. It was concluded that the simultaneous application of MBG-Ag sealing and Er:YAG laser treatment can prevent the drawbacks of their independent uses, resulting in a superior form of treatment for dentin hypersensitivity.

scholarly journals Efficacy of remineralizing agents to occlude dentinal tubules in primary teeth subjected to dentin hypersensitivity in vitro: SEM study

2020 ◽  
Vol 9 (1) ◽  
pp. 354 ◽  
Author(s):  
MebinGeorge Mathew ◽  
AshuJagdish Soni ◽  
MdMuzammil Khan ◽  
Afreen Kauser ◽  
VunnamSri Sai Charan ◽  
...  
Keyword(s):  
Primary Teeth ◽  
Dentin Hypersensitivity ◽  
Dentinal Tubules ◽  
Sem Study

scholarly journals In vitro study of dentin hypersensitivity treated by whitlockite glass-ceramics

2017 ◽  
Author(s):  
Amanda C. Juraski
Keyword(s):  
Bioactive Glass ◽  
Artificial Saliva ◽  
Third Molar ◽  
Glass Ceramics ◽  
In Vitro Study ◽  
Positive Control ◽  
Negative Control ◽  
Dentin Hypersensitivity ◽  
Human Teeth

AbstractThe aim of this study is to evaluate the potential of a bioactive glass based on the 3CaO.P2O5-SiO2MgO-system and its glassceramics containing whitlockite on the remineralization of dentin as a possible treatment to dentin hypersensitivity. For that, 40 third molar human teeth were artificially demineralized and randomly distributed in 4 groups (n = 10): G1 - Negative Control (no treatment), G2 - Positive Control (treated by Bioglass® 45S5), G3 – BG (treated by bioactive glass based on 3CaO.P2O5-SiO2-MgOsystem), and G4 – BGC (treated by bioactive whitlockite glass-ceramics). After treatment, the samples were emerged in artificial saliva and stored for 7 days in a controlled temperature of 37ºC. After that, scanning electron microscopy (SEM) and atomic force microscopy (AFM) was used to evaluate samples morphology. The analysis confirmed the formation of hydroxyapatite on the surface of all the biomaterials studied, that in the dentine specimens treated by bioactive glass and whitlockite glas-ceramic most of the dentinal tubules were completely occluded.

scholarly journals N-Terminal Extension of Human Immunodeficiency Virus Capsid Protein Converts the In Vitro Assembly Phenotype from Tubular to Spherical Particles

1998 ◽  
Vol 72 (6) ◽  
pp. 4798-4810 ◽  
Author(s):  
Ingolf Gross ◽  
Heinz Hohenberg ◽  
Carola Huckhagel ◽  
Hans-Georg Kräusslich
Keyword(s):  
Human Immunodeficiency Virus ◽  
Spherical Shape ◽  
Proteolytic Cleavage ◽  
Spherical Particles ◽  
Molar Ratio ◽  
Immunodeficiency Virus ◽  
In Vitro Assembly ◽  
The Matrix ◽  
Cylindrical Particles

ABSTRACT Expression of retroviral Gag polyproteins is sufficient for morphogenesis of virus-like particles with a spherical immature protein shell. Proteolytic cleavage of Gag into the matrix (MA), capsid (CA), nucleocapsid (NC), and p6 domains (in the case of human immunodeficiency virus [HIV]) leads to condensation to the mature cone-shaped core. We have analyzed the formation of spherical or cylindrical particles on in vitro assembly of purified HIV proteins or inside Escherichia coli cells. CA protein alone yielded cylindrical particles, while all N-terminal extensions of CA abolished cylinder formation. Spherical particles with heterogeneous diameters or amorphous protein aggregates were observed instead. Extending CA by 5 amino acids was sufficient to convert the assembly phenotype to spherical particles. Sequences C-terminal of CA were not required for sphere formation. Proteolytic cleavage of N-terminally extended CA proteins prior to in vitro assembly led to the formation of cylindrical particles, while proteolysis of in vitro assembly products caused disruption of spheres but not formation of cylinders. In vitro assembly of CA and extended CA proteins in the presence of cyclophilin A (CypA) at a CA-to-CypA molar ratio of 10:1 yielded significantly longer cylinders and heterogeneous spheres, while higher concentrations of CypA completely disrupted particle formation. We conclude that the spherical shape of immature HIV particles is determined by the presence of an N-terminal extension on the CA domain and that core condensation during virion maturation requires the liberation of the N terminus of CA.

scholarly journals The influence of vinegars on exposure of dentinal tubules: a SEM evaluation

2004 ◽  
Vol 18 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Daniela Leal Zandim ◽  
Fernanda Oliveira Bello Corrêa ◽  
José Eduardo Cezar Sampaio ◽  
Carlos Rossa Júnior
Keyword(s):  
Dietary Habits ◽  
In Vitro Study ◽  
Smear Layer ◽  
Control Group ◽  
Dentin Hypersensitivity ◽  
Human Teeth ◽  
Painful Condition ◽  
Dentinal Tubules ◽  
Significant Difference

Dentin hypersensitivity is a common painful condition observed in clinics. Dietary habits have been much associated with its development and persistence during and following periodontal treatment. The aim of this in vitro study was to evaluate the influence of vinegars on the removal of smear layer and exposure of dentinal tubules. Extracted human teeth were submitted to manual scaling with Gracey curettes in order to remove the cementum as well as to form a smear layer. Dentin samples with 3 mm² were obtained and distributed into six experimental groups: one control and five types of vinegars (alcohol, apple, rice, white wine and balsamic). Each group included two methods of vinegar application: topical and friction. After routine preparation for SEM analysis, photomicrographs were assessed by a calibrated and blind examiner using an appropriate index system. Kruskal-Wallis test indicated a significant influence of vinegars on smear layer removal. There was a statistically significant difference between groups treated with apple, white and rice vinegars and the control group (p < 0.05). Nevertheless, Mann-Whitney test indicated that removal of smear layer did not vary with the method of application (topical versus friction) for any of the tested substances. We can conclude that the contact of vinegar may remove smear layer and expose dentinal tubules, regardless of the type of application. However, balsamic vinegar was associated with less removal of smear layer after both methods of application.

scholarly journals Mesoporous Bioactive Glass Combined with Graphene Oxide Quantum Dot as a New Material for a New Treatment Option for Dentin Hypersensitivity

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 621
Author(s):  
Sung-Ae Son ◽  
Dong-Hyun Kim ◽  
Kyung-Hyeon Yoo ◽  
Seog-Young Yoon ◽  
Yong-Il Kim
Keyword(s):  
Graphene Oxide ◽  
Electron Microscope ◽  
Quantum Dot ◽  
Bioactive Glass ◽  
Pore Size ◽  
Field Emission ◽  
Dentin Hypersensitivity ◽  
Dentinal Tubules ◽  
New Material ◽  
Mesoporous Bioactive Glass

Dentin hypersensitivity is one of the most common clinical conditions usually associated with exposed dentinal tubules. The purpose of this study was to identify the potential of a graphene oxide quantum dot coating for mesoporous bioactive glass nanoparticles as a new material for the treatment of dentin hypersensitivity by investigating its mineralization activity and dentinal tubules sealing. Mesoporous bioactive glass nanoparticle was fabricated by modified sol-gel synthesis. X-ray diffraction was performed to characterize the synthesized nanoparticle Fourier transform infra-red spectroscopy investigated the functionalized surfaces. The distribution of the specific surface area and the pore size was measure by Pore size analysis. The morphology of sample was observed by Field Emission Scanning Electron Microscope (FESEM) and Field Emission Transmission Electron Microscope (FETEM). After disk-shaped specimens of mesoporous bioactive glass nanoparticles and graphene oxide quantum dot coated mesoporous bioactive glass nanoparticles (n = 3) were soaked in the simulated body fluid for 0, 1, 5, 10,and 30 days, the amount of ions released was observed to confirm the ionic elution for mineralization. Sensitive tooth model discs (n = 20) were applied with two samples and evaluated the dentinal tubule sealing ability. The spherical mesoporous bioactive glass nanoparticles and graphene oxide quantum dot coated mesoporous bioactive glass nanoparticles with a diameter of about 500 nm were identified through FESEM and FETEM. The ion release capacity of both samples appeared to be very similar. The amount of ion released and in vitro mineralization tests confirmed that graphene oxide quantum dot coating of mesoporous bioactive glass nanoparticles did not inhibit the release of calcium, silicon and phosphate ions, but rather that graphene oxide quantum dot promoted hydroxyapatite formation. In the FESEM image of the sensitive tooth disc surface, it was observed that graphene oxide quantum dot coated mesoporous bioactive glass nanoparticles sealed tightly the dentinal tubules. The graphene oxide quantum dot coating of mesoporous bioactive glass nanoparticles not only showed the excellent dentinal sealing ability but also rapidly promoted mineralization while minimizing the size increase by coating the mesoporous bioactive glass nanoparticles.

scholarly journals Effectiveness of Thymoquinone and Fluoridated Bioactive Glass/Nano-Oxide Contained Dentifrices on Abrasion and Dentine Tubules Occlusion: An Ex Vivo Study

2020 ◽  
Vol 14 (01) ◽  
pp. 045-054 ◽  
Author(s):  
Daud Anthoney ◽  
Shahreen Zahid ◽  
Hina Khalid ◽  
Zohaib Khurshid ◽  
Asma Tufail Shah ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Ex Vivo ◽  
Three Dimensional ◽  
International Standards ◽  
P Value ◽  
Dentin Hypersensitivity ◽  
Dentinal Tubules ◽  
Tooth Structure ◽  
Nano Zinc Oxide ◽  
Nano Zinc

Abstract Objectives Dentin hypersensitivity (DH) is mainly due to the loss and replenishment of minerals from tooth structure, where the lost minerals can be rehabilitated with a biomimetic approach. The objectives were to determine the relative dentin abrasivity (RDA) of experimental (EXT) dentifrices and to determine the efficacy to occlude dentinal tubules. Materials and Methods Experimental dentifrices contained nano-fluoridated bioactive glass (n-FBG: 1.5 wt.% [EXT-A], 2.5 wt.% [EXT-B], and 3.5 wt.% [EXT-C]), nano-zinc oxide (n-ZnO), and thymoquinone as active agents. Bovine dentin blocks were subjected to brushing treatments as per groups, that is, distilled water; commercial dentifrice (control, CT); EXT toothpastes; and EXT-D without active agents. Samples were tested for three-dimensional (3D) abrasion analysis according to ISO-11609:2010 (International Organization for Standardization [ISO]). The roughness average (Ra), RDA, surface topography, and elemental compositions were investigated. Statistical Analysis One-way analysis of variance (ANOVA) with post-hoc Tukey’s and Tamhane’s test was performed for characterizations using Statistical Package for the Social Sciences (SPSS) version 21. The result was considered significant with p-value ≤ 0.05. Results Comparisons of Ra differed significantly between all groups with p < 0.05 except CT and EXT-A. The RDA values of EXT-A, EXT-B, and EXT-C were calculated as 74.04, 84.26, and 116.24, respectively, which were well within the acceptable limit set by international standards. All n-FBG containing dentifrices demonstrated uniform occlusion of dentinal tubules; however, highly concentrated EXT dentifrices showed more occlusion. Conclusions Acceptable range of RDA and superior occlusion of tubules by novel dentifrices suggest that it may be recommended for treating DH.

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