scholarly journals Cardiovascular and Respiratory Toxicity of Protamine Sulfate in Zebrafish and Rodent Models

Pharmaceutics ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 359
Author(s):  
Joanna Miklosz ◽  
Bartlomiej Kalaska ◽  
Piotr Podlasz ◽  
Małgorzata Chmielewska-Krzesińska ◽  
Miłosz Zajączkowski ◽  
...  
Keyword(s):  
Anticoagulant Activity ◽  
Heart Tissue ◽  
Protamine Sulfate ◽  
Blood Oxygen ◽  
Careful Monitoring ◽  
Blood Oxygen Saturation ◽  
Respiratory Parameters ◽  
Morphological Defects ◽  
Rats And Mice ◽  
Herg Channels

Protamine sulfate (PS) is the only available option to reverse the anticoagulant activity of unfractionated heparin (UFH), however it can cause cardiovascular and respiratory complications. We explored the toxicity of PS and its complexes with UFH in zebrafish, rats, and mice. The involvement of nitric oxide (NO) in the above effects was investigated. Concentration–dependent lethality, morphological defects, and decrease in heart rate (HR) were observed in zebrafish larvae. PS affected HR, blood pressure, respiratory rate, peak exhaled CO2, and blood oxygen saturation in rats. We observed hypotension, increase of HR, perfusion of paw vessels, and enhanced respiratory disturbances with increases doses of PS. We found no effects of PS on human hERG channels or signs of heart damage in mice. The hypotension in rats and bradycardia in zebrafish were partially attenuated by the inhibitor of endothelial NO synthase. The disturbances in cardiovascular and respiratory parameters were reduced or delayed when PS was administered together with UFH. The cardiorespiratory toxicity of PS seems to be charge–dependent and involves enhanced release of NO. PS administered at appropriate doses and ratios with UFH should not cause permanent damage of heart tissue, although careful monitoring of cardiorespiratory parameters is necessary.

scholarly journals Trajectories of Key Physiological Parameters in COVID-19 Patients Using Continuous Remote Monitoring and Health AI

2020 ◽  
Author(s):  
Arik Eisenkraft ◽  
Yasmin Maor ◽  
Keren Constantini ◽  
Nir Goldstein ◽  
Dean Nachman ◽  
...  
Keyword(s):  
Remote Monitoring ◽  
Vital Signs ◽  
Blood Oxygen ◽  
Cardiorespiratory System ◽  
Blood Oxygen Saturation ◽  
Frequent Monitoring ◽  
Respiratory Parameters ◽  
Multi Center Study ◽  
First Time ◽  
Worse Prognosis

Abstract Coronavirus disease 2019 (COVID-19) exerts deleterious effects on the cardiorespiratory system, leading to worse prognosis in the most effected. The aim of this retrospective multi-center study was to describe the variability of key cardiopulmonary vitals amongst hospitalized COVID-19 patients, measured every 15 minutes using a novel wearable chest-monitor. A total of 492 patients were included, with >3 million measurements collected including heart rate, systolic and diastolic blood pressure, cardiac output, cardiac index, systemic vascular resistance, respiratory rate, blood oxygen saturation, and body temperature. We show differential trajectories of these vital signs, apparent within the first 24hrs of monitoring. Importantly, we show for the first time that cardiovascular deterioration appears early after admission and in parallel with changes in the respiratory parameters, and identify sub-populations at high risk. Combining frequent monitoring using wearable technology with advanced big data and AI analysis tools may aid early detection of deterioration of COVID-19 patients.

scholarly journals Monitoring of Cardiorespiratory Parameters in Rats—Validation Based on Pharmacological Stimulation

2021 ◽  
Vol 14 (12) ◽  
pp. 1223
Author(s):  
Joanna Miklosz ◽  
Bartlomiej Kalaska ◽  
Stanislaw Zajaczkowski ◽  
Dariusz Pawlak ◽  
Andrzej Mogielnicki
Keyword(s):  
Blood Pressure ◽  
Body Temperature ◽  
Oxygen Saturation ◽  
Respiratory Rate ◽  
Blood Perfusion ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Toxicity Studies ◽  
Respiratory Parameters ◽  
Safety And Toxicity

The methods used in preclinical studies should minimize the suffering and the number of animals but still provide precise and consistent results enabling the introduction of drug candidates into the phase of clinical trials. Thus, we aimed to develop a method allowing us to perform preliminary safety and toxicity studies of candidates for human medicines, while reducing the number of animals. We have devised a method based on a combination of two devices: Plugsys (Transonics System Inc., Ithaca, NY, USA) and PhysioSuite (Kent Scientific Corporation, Torrington, CT, USA), which allow simultaneous registration of nine circulatory and respiratory parameters, and body temperature. Vehicle and adrenaline, or nitroglycerin, as reference substances were administered into the right femoral vein of Wistar rats. Physiological conditions were registered over 60 min after drug administration by measuring systolic, diastolic and mean blood pressure, heart rate (HR), blood perfusion of paw vessels, blood oxygen saturation, respiratory rate, average and peak exhaled CO2, and body temperature. Blood pressure was measured by cannula placed in the left common carotid artery and connected to the pressure transducer (Plugsys). The other parameters were measured by the PhysioSuite. Adrenaline-induced immediate dose-related hypertension and nitroglycerin hypotension were correlated with the change in blood perfusion. They both increased HR. Adrenaline decreased blood oxygen saturation and slightly affected respiratory parameters, while nitroglycerin caused a progressive increase in respiratory rate and a decrease in the peak of exhaled CO2. Our method may become an inseparable part of the preliminary safety and toxicity studies of tested drugs, while being an important step towards improving animal welfare.

scholarly journals PSIX-9 Impacts of bunk management on feedlot performance, carcass characteristics, hydrogen sulfide concentration and blood oxygen saturation in steers fed 25 or 50% modified distillers grains plus solubles

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 422-422
Author(s):  
Rebecca L Moore ◽  
Cierrah J Kassetas ◽  
Leslie A LeKatz ◽  
Bryan W Neville
Keyword(s):  
Hydrogen Sulfide ◽  
Oxygen Saturation ◽  
Management Strategy ◽  
Distillers Grains ◽  
Feed Ratio ◽  
Sulfide Concentration ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Feedlot Performance ◽  
Arterial Blood

Abstract One hundred and twenty-six yearling angus steers (initial body weight 445.87 ± 7.13 kg) were utilized in a 2 x 2 factorial design to evaluate the impacts of bunk management and modified distillers grains plus solubles (mDGS) inclusion on feedlot performance, hydrogen sulfide concentrations and blood oxygen saturation. Treatments included bunk management strategy either control bunk management (CON; clean bunks at the time of next day’s feeding) or long bunk management (LONG; feed remaining at time of next day’s feeding), and two inclusion rates of mDGS either 25% or 50% (DM Basis). On d 0, 7, 14, 21, 28 and 35 rumen gas samples were collected via rumenocentesis, and arterial blood samples were collected on two steers from each pen. No differences (P ≥ 0.09) were observed for dry matter intake, average daily gain and gain-to-feed ratio for bunk management or mDGS inclusion. Hot carcass weight, ribeye area, marbling score and quality grade were not affected (P ≥ 0.48) by either bunk management or mDGS inclusion. Back fat was greater (P = 0.04) for CON steers compared to LONG (1.30 vs 1.12 ± 0.05cm, respectively), but was not affected (P = 0.59) by mDGS inclusion. Steers on CON had greater (P = 0.03) yield grades compared to LONG (3.21 vs 2.96 ± 0.11, respectively). Bunk management strategy did not impact hydrogen sulfide concentrations or blood oxygen saturation (P = 0.82). Hydrogen sulfide concentrations increased (P < 0.001) with increasing mDGS inclusion. Blood oxygen saturation was influenced by day of sampling (P = 0.01). Blood oxygen saturation was not affected (P = 0.07) by mDGS inclusion. The fact that ruminal hydrogen sulfide concentrations increased while blood oxygen saturation remained similar raises questions about the quantity of hydrogen sulfide and metabolic fate of excess hydrogen sulfide in the blood of ruminant animals.

Pulse Oximetry and Breathing Training1

Biofeedback ◽  
2012 ◽  
Vol 40 (4) ◽  
pp. 137-141 ◽  
Author(s):  
Christopher Gilbert
Keyword(s):  
Oxygen Saturation ◽  
Pulse Oximetry ◽  
Medical Condition ◽  
Noninvasive Monitoring ◽  
Control Of Breathing ◽  
Oxygen Absorption ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Training Control ◽  
Pulse Oximeters

Small pulse oximeters have become widely available and can be useful for noninvasive monitoring of blood oxygen saturation by nonmedical personnel. When training control of breathing, an oximeter helps to reassure clients who hyperventilate that their oxygenation is adequate, offsetting their perception that they are not getting enough air. Low saturation may indicate a medical condition that impairs oxygen absorption. In that case, hyperventilation is a biological compensation that should not be tampered with.

Blood Oxygen Saturation Measurement Using Polarization-Dependent Optical Sectioning

2017 ◽  
Vol 17 (12) ◽  
pp. 3900-3908 ◽  
Author(s):  
Deepak Mishra ◽  
Neha Priyadarshini ◽  
Supriya Chakraborty ◽  
Mukul Sarkar
Keyword(s):  
Oxygen Saturation ◽  
Blood Oxygen ◽  
Optical Sectioning ◽  
Blood Oxygen Saturation ◽  
Saturation Measurement

scholarly journals Optical Measurement of Blood Oxygen Saturation of Dental Pulp

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Satoko Kakino ◽  
Shinya Kushibiki ◽  
Azusa Yamada ◽  
Zenzo Miwa ◽  
Yuzo Takagi ◽  
...  
Keyword(s):  
Light Intensity ◽  
Oxygen Saturation ◽  
Dental Pulp ◽  
Optical Measurement ◽  
Blood Oxygen ◽  
Arterial Pulse ◽  
Blood Oxygen Saturation ◽  
Visible Wavelengths

The applicability of arterial pulse oximetry to dental pulp was demonstrated using in vitro and in vivo measurements. First, porcine blood of known oxygen saturation (SO2) was circulated through extracted human upper incisors, while transmitted-light plethysmography was performed using three different visible wavelengths. From the light intensity waveforms measured in vitro, a parameter that is statistically correlated to SO2 was calculated using the pulsatile/nonpulsatile component ratios of two wavelengths for different SO2. Then, values were measured in vivo for living incisors, and the corresponding SO2 values were calculated using the results of in vitro measurements. The estimated SO2 values of the upper central incisors measured in vivo were from 71.0 to 92.7%. This study showed the potential to measure the oxygen saturation changes to identify the sign of pulpal inflammation.

scholarly journals A Comparison of Cerebral Hemovelocity and Blood Oxygen Saturation Levels during Vigilance Performance

2010 ◽  
Vol 54 (18) ◽  
pp. 1345-1349 ◽  
Author(s):  
Matthew E. Funke ◽  
Joel S. Warm ◽  
Gerald Matthews ◽  
Michael Riley ◽  
Victor Finomore ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Vigilance Performance ◽  
Cerebral Hemovelocity

USE OF TELECOMMUNICATIONS TO REDUCE THE IMPACT OF THE COVID-19 PANDEMIC

2021 ◽  
Vol 2 (1) ◽  
pp. 38-43
Author(s):  
Abdullajon Komilov ◽  
◽  
Madinabonu Sultonova ◽  
Durdona Orifjonova
Keyword(s):  
Oxygen Saturation ◽  
Chronic Diseases ◽  
Direct Contact ◽  
Healthcare Professionals ◽  
Automatic Monitoring ◽  
Saturation Level ◽  
Blood Oxygen ◽  
Blood Oxygen Saturation ◽  
Oxygen Saturation Level ◽  
The Impact

Today, the COVID-19 pandemic is one of the most pressing problems facing humanity. Therefore, reducing the population's level of infection with this virus is one of our government's main tasks.Therefore, it is necessary to isolate patients with chronic diseases as much as possible. Clients are more likely to be infected with caronavirus due to their age and the presence of underlying medical conditions.Limiting direct contact between such patients and observing healthcare professionals significantly reduces the patient's chances of contracting caronavirus.The article proposes a device design that allows for remote automatic monitoring of patients' condition being treated at home by an observing medical professional.With the proposed device's help, the patient's blood oxygen saturation level is automatically and remotely controlled. The device is built on an Arduino board.The use of the device greatly reduces the likelihood of contracting caronavirus in patients receiving home treatment.This could be one of the most important decisions in maintaining the health of the population today.

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