Exogenous Gibberellic Acid or Dilute Bee Honey Boosts Drought Stress Tolerance in Vicia faba by Rebalancing Osmoprotectants, Antioxidants, Nutrients, and Phytohormones

The use of growth regulators such as gibberellic acid (GA3) and biostimulants, including diluted bee honey (Db-H) can improve drought tolerance in many crops, including the faba bean (Vicia faba L.). Db-H contains high values of osmoprotectants, mineral nutrients, vitamins, and many antioxidants making it an effective growth regulator against environmental stress effects. Therefore, the present study was planned to investigate the potential improvement in the faba bean plant performance (growth and productivity) under full watering (100% of crop evapotranspiration (ETc)) and drought stress (60% of ETc) by foliar application of GA3 (20 mg L−1) or Db-H (20 g L−1). The ameliorative impacts of these growth regulators on growth, productivity, physio-biochemical attributes, nutrient status, antioxidant defense system, and phytohormones were evaluated. GA3 or Db-H attenuated the negative influences of drought stress on cell membrane stability, ion leakage, relative water content, nutrient status, leaf pigments related to photosynthesis (chlorophylls and carotenoids), and efficiency of the photosystem II (PSII in terms of Fv/Fm and performance index), thus improving faba bean growth, green pod yield, and water use efficiency. Drought stress caused an abnormal state of nutrients and photosynthetic machinery due to increased indicators of oxidative stress (malondialdehyde (MDA), hydrogen peroxide (H2O2) and superoxide (O2•−)), associated with increased osmoprotectants (proline, glycine betaine, soluble sugars, and soluble protein), non-enzymatic antioxidants (ascorbic acid, glutathione, and α-tocopherol), and enzymatic antioxidant activities (superoxide dismutase, catalase, glutathione reductase, and ascorbate peroxidase). However, foliar-applied GA3 or Db-H mediated further increases in osmoprotectants, antioxidant capacity, GA3, indole-3-acetic acid, and cytokinins, along with decreased levels of MDA and abscisic acid. These results suggest the use of GA3 or Db-H at the tested concentrations to mitigate drought-induced damage in bean plants to obtain satisfactory growth and productivity under a water deficit of up to 40%.

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EFFECT OF SALICYLIC ACID AND ABSCISIC ACID ON MORPHO-PHYSIOLOGICAL AND ANATOMICAL CHARACTERS OF FABA BEAN PLANTS (Vicia faba L.) UNDER DROUGHT STRESS

Journal of Plant Production ◽

10.21608/jpp.2015.52096 ◽

2015 ◽

Vol 6 (11) ◽

pp. 1771-1788 ◽

Cited By ~ 9

Author(s):

Kh. Abdelaal

Keyword(s):

Salicylic Acid ◽

Abscisic Acid ◽

Drought Stress ◽

Vicia Faba ◽

Faba Bean ◽

Bean Plants ◽

Vicia Faba L ◽

Anatomical Characters

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Variation in antioxidant metabolism of faba bean (Vicia faba) under drought stress induced by polyethylene glycol reveals biochemical markers associated with antioxidant defense

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology ◽

10.1080/11263504.2020.1785964 ◽

2020 ◽

pp. 1-10

Author(s):

Ghassen Abid ◽

Rim Nefissi Ouertani ◽

Yordan Muhovski ◽

Salwa Harzalli Jebara ◽

Yassine Hidri ◽

...

Keyword(s):

Polyethylene Glycol ◽

Drought Stress ◽

Vicia Faba ◽

Faba Bean ◽

Biochemical Markers ◽

Antioxidant Defense ◽

Antioxidant Metabolism

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The cropping system matters – Contrasting responses of winter faba bean (Vicia faba L.) genotypes to drought stress

Journal of Plant Physiology ◽

10.1016/j.jplph.2021.153463 ◽

2021 ◽

pp. 153463

Author(s):

Annika Meißner ◽

Sandra Granzow ◽

Franziska Wemheuer ◽

Birgit Pfeiffer

Keyword(s):

Drought Stress ◽

Vicia Faba ◽

Faba Bean ◽

Cropping System ◽

Vicia Faba L

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Physiological and Molecular Characterization of Faba bean (Vicia faba L.) Genotypes for Adaptation to Drought Stress

Journal of Agronomy and Crop Science ◽

10.1111/jac.12110 ◽

2014 ◽

Vol 201 (6) ◽

pp. 401-409 ◽

Cited By ~ 6

Author(s):

S. S. Alghamdi ◽

A. M. Al-Shameri ◽

H. M. Migdadi ◽

M. H. Ammar ◽

E. H. El-Harty ◽

...

Keyword(s):

Drought Stress ◽

Molecular Characterization ◽

Vicia Faba ◽

Faba Bean ◽

Vicia Faba L ◽

Adaptation To Drought

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Drought stress impact on leaf proteome variations of faba bean (Vicia faba L.) in the Qinghai–Tibet Plateau of China

3 Biotech ◽

10.1007/s13205-018-1088-3 ◽

2018 ◽

Vol 8 (2) ◽

Cited By ~ 13

Author(s):

Ping Li ◽

Yanxia Zhang ◽

Xuexia Wu ◽

Yujiao Liu

Keyword(s):

Drought Stress ◽

Vicia Faba ◽

Faba Bean ◽

Tibet Plateau ◽

Vicia Faba L ◽

Leaf Proteome ◽

Qinghai Tibet Plateau

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Exogenous Application of Gibberellic Acid Improves the Maize Crop Productivity Under Scarce and Sufficient Soil Moisture Condition

Cercetari Agronomice in Moldova ◽

10.1515/cerce-2017-0036 ◽

2017 ◽

Vol 50 (4) ◽

pp. 65-73 ◽

Cited By ~ 1

Author(s):

N. Sarwar ◽

O. Farooq ◽

K. Mubeen ◽

A. Wasaya ◽

W. Nouman ◽

...

Keyword(s):

Growth Rate ◽

Drought Stress ◽

Drought Tolerance ◽

Gibberellic Acid ◽

Leaf Area ◽

Crop Yield ◽

Growth Regulators ◽

Drought Condition ◽

Irrigation Level ◽

Exogenous Application

AbstractDrought stress creates imbalance or deficiency of some growth regulators in plants, which leads toward reduced crop yield. Gibberellic acid is one of the most important growth regulators in plants, which improve drought tolerance in plants under optimum concentration. A field experiment was conducted under exogenous application of gibberellic acid under normal or drought condition and with or without gibberellic acid application. Crop growth and yield parameters were assesses during the experimentation. Study revealed that crop reduced growth in term of leaf area index (LAI), leaf area duration (LAD), crop growth rate (CGR), net assimilation rate (NAR) and total dry matter (TDM) under drought condition, while these parameters were improved with gibberellic acid application. Similary, improved growth rate resulted in better performance of yield attributes (cob length, cob diameter, grains per cob, grain weight and yield). Gibberellic acid application improved the crop performance at optimum irrigation, as well as under reduced irrigation. Although highest crop yield was recorded with gibberellic acid application under optimum irrigation level, while its application under drought stress improved crop tolerance and resulted in better crop yield, similar to optimum irrigation level. Exogenous application of gibberellic acid not only improved the drought tolerance in maize, but also increased the crop yield under normal condition.

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Characterization of drought stress-responsive root transcriptome of faba bean (Vicia faba L.) using RNA sequencing

3 Biotech ◽

10.1007/s13205-018-1518-2 ◽

2018 ◽

Vol 8 (12) ◽

Cited By ~ 3

Author(s):

Salem S. Alghamdi ◽

Muhammad A. Khan ◽

Megahed H. Ammar ◽

Qiwei Sun ◽

Lihua Huang ◽

...

Keyword(s):

Drought Stress ◽

Rna Sequencing ◽

Vicia Faba ◽

Faba Bean ◽

Root Transcriptome ◽

Vicia Faba L

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Ecotypic Morphological and Physio-Biochemical Responses of Two Differentially Adapted Forage Grasses, Cenchrus ciliaris L. and Cyperus arenarius Retz. to Drought Stress

Sustainability ◽

10.3390/su13148069 ◽

2021 ◽

Vol 13 (14) ◽

pp. 8069

Author(s):

Muhammad Adeel Ghafar ◽

Nudrat Aisha Akram ◽

Muhammad Hamzah Saleem ◽

Jianyong Wang ◽

Leonard Wijaya ◽

...

Keyword(s):

Drought Stress ◽

Soluble Sugars ◽

Field Capacity ◽

Research Area ◽

Grass Species ◽

Enzymatic Antioxidants ◽

Forage Grasses ◽

Cenchrus Ciliaris ◽

Salt Range ◽

Water Scarce

Crop performance and yield are the results of genotypic expression as modulated by continuous interaction with the environment. Among the environmental aspects, drought and salinity are the most important factors, which limit the forages, including grasses, on a global basis. Grass species have the ability to grow under low water conditions and can produce high dry yield, proteins, and energy in areas exposed to drought stress. For this purpose, we conducted the present study to understand the response of forage grasses under drought stress from two different regions (Salt Range and Faisalabad) of Punjab, Pakistan. Two ecotypes of each grass species (Cenchrus ciliaris L. and Cyperus arenarius Retz.) were grown in pots at the botanical research area, Government College University Faisalabad, Pakistan. A group of plants were subjected to drought stress (60% field capacity) and controlled (100% field capacity) after three weeks of seed germination. The results from the present study depicted that the fresh and dry weights of root and shoot were decreased significantly under drought conditions. Moreover, C. ciliaris of the Salt Range area showed more resistance and higher growth production under drought stress. The chlorophyll (a and b) contents were also decreased significantly, while MDA, total soluble sugars, and proline levels were increased significantly under water-limited environments in the C. arenarius of Salt Range area. Enzymatic antioxidants (superoxidase dismutase (SOD) and peroxidase (POD)) and leaf Na+ were significantly raised in C. arenarius under drought stress collected from the Faisalabad region. Cenchrus ciliaris showed higher level of H2O2, total soluble proteins, glycinebetaine, catalase (CAT) and POD compared to C. arenarius. It also retained more leaf and root Ca2+, and root K+ under drought stress. It was concluded from the study that C. ciliaris is more resistant to drought in biomass production collected from the Salt Range area. The results suggested that C. ciliaris can be more widely used as a forage grass under water-scarce conditions as compared to C. arenarius.

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