scholarly journals Phosphite Reduces the Predation Impact of Poterioochromonas malhamensis on Cyanobacterial Culture

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1361
Author(s):  
Narumi Toda ◽  
Hiroki Murakami ◽  
Akihiro Kanbara ◽  
Akio Kuroda ◽  
Ryuichi Hirota
Keyword(s):  
Community Analysis ◽  
Environmental Water ◽  
Microbial Community Analysis ◽  
Grazing Impact ◽  
Competitive Growth ◽  
Microalgal Cultivation ◽  
Suppression Effect ◽  
Commercial Scale ◽  
Poterioochromonas Malhamensis ◽  
The Impact

Contamination by the predatory zooplankton Poterioochromonas malhamensis is one of the major threats that causes catastrophic damage to commercial-scale microalgal cultivation. However, knowledge of how to manage predator contamination is limited. Previously, we established a phosphite (Pt)-based culture system by engineering Synechococcus elongatus, which exerted a competitive growth advantage against microbial contaminants that compete with phosphate source. Here, we examined whether Pt is effective in suppressing predator-type contamination. Co-culture experiment of Synechococcus with isolated P. malhamensis revealed that, although an addition of Pt at low concentrations up to 2.0 mM was not effective, increased dosage of Pt (~20 mM) resulted in the reduced grazing impact of P. malhamensis. By using unsterilized raw environmental water collected from rivers or ponds, we found that the suppression effect of Pt was dependent on the type of environmental water used. Eukaryotic microbial community analysis of the cultures using environmental water samples revealed that Paraphysomonas, a colorless Chrysophyceae, emerged and dominated under high-Pt conditions, suggesting that Paraphysomonas is insensitive to Pt compared to P. malhamensis. These findings may provide a clue for developing a strategy to reduce the impact of grazer contamination in commercial-scale microalgal cultivation.

scholarly journals To rarefy or not to rarefy: Enhancing microbial community analysis through next-generation sequencing

2020 ◽  
Author(s):  
Ellen S. Cameron ◽  
Philip J. Schmidt ◽  
Benjamin J.-M. Tremblay ◽  
Monica B. Emelko ◽  
Kirsten M. Müller
Keyword(s):  
Microbial Community ◽  
Community Analysis ◽  
Amplicon Sequencing ◽  
Microbial Community Analysis ◽  
List Type ◽  
Sequencing Data ◽  
Water And Wastewater Treatment ◽  
Library Size ◽  
Size Selection ◽  
The Impact

AbstractThe application of amplicon sequencing in water research provides a rapid and sensitive technique for microbial community analysis in a variety of environments ranging from freshwater lakes to water and wastewater treatment plants. It has revolutionized our ability to study DNA collected from environmental samples by eliminating the challenges associated with lab cultivation and taxonomic identification. DNA sequencing data consist of discrete counts of sequence reads, the total number of which is the library size. Samples may have different library sizes and thus, a normalization technique is required to meaningfully compare them. The process of randomly subsampling sequences to a selected normalized library size from the sample library—rarefying—is one such normalization technique. However, rarefying has been criticized as a normalization technique because data can be omitted through the exclusion of either excess sequences or entire samples, depending on the rarefied library size selected. Although it has been suggested that rarefying should be avoided altogether, we propose that repeatedly rarefying enables (i) characterization of the variation introduced to diversity analyses by this random subsampling and (ii) selection of smaller library sizes where necessary to incorporate all samples in the analysis. Rarefying may be a statistically valid normalization technique, but researchers should evaluate their data to make appropriate decisions regarding library size selection and subsampling type. The impact of normalized library size selection and rarefying with or without replacement in diversity analyses were evaluated herein.Highlights▪ Amplicon sequencing technology for environmental water samples is reviewed▪ Sequencing data must be normalized to allow comparison in diversity analyses▪ Rarefying normalizes library sizes by subsampling from observed sequences▪ Criticisms of data loss through rarefying can be resolved by rarefying repeatedly▪ Rarefying repeatedly characterizes errors introduced by subsampling sequences

scholarly journals A critical perspective on interpreting amplicon sequencing data in soil ecological research

2021 ◽  
Author(s):  
Lauren V. Alteio ◽  
Joana Séneca ◽  
Alberto Canarini ◽  
Roey Angel ◽  
Ksenia Guseva ◽  
...  
Keyword(s):  
Statistical Power ◽  
Marker Gene ◽  
Community Analysis ◽  
Amplicon Sequencing ◽  
Data Availability ◽  
Microbial Community Analysis ◽  
Sequencing Data ◽  
Spatio Temporal ◽  
Complementary Techniques ◽  
The Impact

Microbial community analysis via marker gene amplicon sequencing has become a routine method in the field of soil research. In this perspective, we discuss technical challenges and limitations of amplicon sequencing studies in soil and present statistical and experimental approaches that can help addressing the spatio-temporal complexity of soil and the high diversity of organisms therein. We illustrate the impact of compositionality on the interpretation of relative abundance data and discuss effects of sample replication on the statistical power in soil community analysis. Additionally, we argue for the need of increased study reproducibility and data availability, as well as complementary techniques for generating deeper ecological insights into microbial roles and our understanding thereof in soil ecosystems. At this stage, we call upon researchers and specialized soil journals to consider the current state of data analysis, interpretation and availability to improve the rigor of future studies.

scholarly journals ENVIRONMENTAL PERFORMANCE OF MICROBIAL FUEL CELL BASED LIVE SLUDGE FOR VOLTAGE PRODUCTION AND CONGO RED DYE REMOVAL

2022 ◽  
Vol 26 (1) ◽  
pp. 27-35
Author(s):  
Nabea M. Mahdi ◽  
◽  
Ahmed H. Ali ◽  
Keyword(s):  
Congo Red ◽  
Microbial Fuel Cells ◽  
Ph Value ◽  
Community Analysis ◽  
Open Circuit ◽  
Organic Substrate ◽  
Microbial Community Analysis ◽  
Maximum Output ◽  
Red Dye ◽  
The Impact

: In this work, Single chamber Microbial fuel cells (SCMFCs) are a versatile technology is depends on the interaction mechanisms of bacteria, to produce bioelectricity simultaneously and treat Congo red (CR) dye from aqueous solution at different pH (6.5-8). Electricity generation from the biodegradable organic substrate (sucrose) accompanied by decolorization of azo dye was investigated in the batch test results showed that more than 99% decolorization demonstrated at UV-Visible Spectrophotometer (500 nm) was achieved within 20 days and maximum output voltage (889 mv) had been obtained in an open circuit at a pH value of 7.5. Microbial community analysis showed that species in live sludge and the impact of bacteria grown on removal and voltage.

scholarly journals Performance and Microbial Community Analysis of an Electrobiofilm Reactor Enhanced by Ferrous-EDTA

ACS Omega ◽  
2021 ◽  
Author(s):  
Nan Liu ◽  
Ying-ying Li ◽  
Du-juan Ouyang ◽  
Chang-yong Zou ◽  
Wei Li ◽  
...  
Keyword(s):  
Microbial Community ◽  
Community Analysis ◽  
Microbial Community Analysis

scholarly journals Development of a combined anaerobic and aerobic membrane bioreactor for wastewater treatment and reclamation in terrestrial-based controlled ecological life support system

2018 ◽  
Vol 19 (3) ◽  
pp. 718-724
Author(s):  
Zhenmin Cheng ◽  
Yuansong Wei ◽  
Min Gao ◽  
Junya Zhang ◽  
Liangchang Zhang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Support System ◽  
Membrane Bioreactor ◽  
Life Support ◽  
Specific Energy Consumption ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Trace Gas ◽  
Life Support System ◽  
Physico Chemical

Abstract A novel wastewater treatment and reuse system (WTRS) combining an anaerobic membrane bioreactor (AnMBR) and an aerobic membrane bioreactor (MBR) with the design capacity of 115 L/d was developed for a terrestrial-based controlled ecological life support system (CELSS). Results clearly showed that the WTRS realized mineralization of organic compounds and reservation of nitrogenous nutrient, therefore converting the effluent into replenishment for the hydroponic system. Trace gas emission from the WTRS could meet requirements for the whole CELSS. Compared with physico-chemical processes, the specific consumables consumption of the WTRS was advantageous but its specific energy consumption is still in need of improvement. Results of microbial community analysis were consistent with the running state of the AnMBR and the MBR.

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