scholarly journals Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1889
Author(s):  
Min-Rui Wang ◽  
Wenlu Bi ◽  
Mukund R. Shukla ◽  
Li Ren ◽  
Zhibo Hamborg ◽  
...  

Cryopreservation is considered an ideal strategy for the long-term preservation of plant genetic resources. Significant progress was achieved over the past several decades, resulting in the successful cryopreservation of the genetic resources of diverse plant species. Cryopreservation procedures often employ in vitro culture techniques and require the precise control of several steps, such as the excision of explants, preculture, osmo- and cryoprotection, dehydration, freeze-thaw cycle, unloading, and post-culture for the recovery of plants. These processes create a stressful environment and cause reactive oxygen species (ROS)-induced oxidative stress, which is detrimental to the growth and regeneration of tissues and plants from cryopreserved tissues. ROS-induced oxidative stresses were documented to induce (epi)genetic and somatic variations. Therefore, the development of true-to-type regenerants of the source germplasm is of primary concern in the application of plant cryopreservation technology. The present article provides a comprehensive assessment of epigenetic and genetic integrity, metabolic stability, and field performance of cryopreserved plants developed in the past decade. Potential areas and the directions of future research in plant cryopreservation are also proposed.

HortScience ◽  
2011 ◽  
Vol 46 (11) ◽  
pp. 1436-1437 ◽  
Author(s):  
Gayle M. Volk ◽  
Christopher M. Richards

Wild plant genetic resources are increasingly becoming valuable for breeding, genomics, and ornamental horticulture programs. Wild relatives of horticultural species may offer desirable traits that are not available in cultivated varieties, but “wilds” often also have traits that are highly undesirable. Advances in comparative genomics and marker-assisted breeding facilitate the inclusion of the valued traits from wild materials in plant breeding programs. As technologies advance, wild plant genetic resources will become even more valuable for future research developments. This serves as an introduction to a series of proceedings articles from the American Society of Horticultural Science meetings in 2010 workshop entitled “Horticultural Value of Wild Genetic Resources.”


Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 367
Author(s):  
Kamalesh Adhikari ◽  
Edwin Bikundo ◽  
Xan Chacko ◽  
Susannah Chapman ◽  
Fran Humphries ◽  
...  

Farmers’ Rights formally appeared in the International Treaty on Plant Genetic Resources for Food and Agriculture (ITPGRFA) as a means of recognising the past, present, and future contributions of farmers in conserving, improving, and making available the plant genetic materials that are important for food and agriculture. Discussions have been underway under the auspices of the ITPGRFA’s Governing Body with the recent Ad Hoc Technical Expert Group on Farmers’ Rights (AHTEG-FR) collecting together views, experiences, and best practices to produce an inventory and options for encouraging, guiding, and promoting the realisation of Farmers’ Rights. While this is useful, this article reports on the outcomes of a workshop that applied a different methodology. Our purpose was to identify what could be and should be the substance of Farmers’ Rights so that the policy substance drives the implementation rather than the AHTEG-FR’s retro-fitting Farmers’ Rights to existing views, best practices, and measures. The contribution of this article is to develop and set out a list of possible substantive Farmers’ Rights as a contribution and foundation for further consultations and negotiations.


Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
JS Sung ◽  
CW Jeong ◽  
YY Lee ◽  
HS Lee ◽  
YA Jeon ◽  
...  

2017 ◽  
Vol 52 (6) ◽  
pp. 1116-1128 ◽  
Author(s):  
N.I. Dzyubenko ◽  
◽  
E.A. Dzyubenko ◽  
E.K. Potokina ◽  
S.V. Bulyntsev ◽  
...  

Author(s):  
N. V. Kulyakina ◽  
G. A. Kuzmitskaya ◽  
T. K. Yurechko

The downy mildew (peronosporosis) is the most dangerous and harmful cucumber disease in the Far-Eastern region. Its harmfulness is the highest during the period of flowering – fruiting, because it may completely ruin the cucumber plants both in open and protected ground. Use of cucumber sorts with effective genes that are resistant to the disease is the most efficient way to fight cucumber peronosporosis. In this connection the publication adduces the facts of study the sorts and hybrids of different ecological and geographical origin from the genetic resources collection of the N.I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR) in comparison with three zoned sorts of DV NIISH selection – Mig, Khabar, Amurchonok. The aim of researches – to identify the sort samples cucumber according to the main economically valuable traits and also to the degree resistance of genetypes to the main disease of the culture in the region – downy mildew (peronosporosis), for allow to select the source material for the creation of sorts and hybrids, highly productive and resistant to biotic and abiotic stress environmental factors in the monsoon climate conditions of Khabarovsk region. The perspective genetypes having both the resistance to the diseases and productivity are not revealed as a result of the evaluation of the collection of cucumber samples according to their susceptibility by fungus diseases and productivity. Slavyanskiy (Belarus) is more resistant to peronosporosis, especially in 2016 year conditions, although it was inferior to the damage degree of disease to the zoned sorts – Mig, Khabar, Amurchonok. This perspective sample is planned to be used as a source of donors of the main economically valuable traits in a further selective work.


2012 ◽  
Vol 163 (6) ◽  
pp. 240-246 ◽  
Author(s):  
Thomas A. Nagel ◽  
Jurij Diaci ◽  
Dusan Rozenbergar ◽  
Tihomir Rugani ◽  
Dejan Firm

Old-growth forest reserves in Slovenia: the past, present, and future Slovenia has a small number of old-growth forest remnants, as well as many forest reserves approaching old-growth conditions. In this paper, we describe some of the basic characteristics of these old-growth remnants and the history of their protection in Slovenia. We then trace the long-term development of research in these old-growth remnants, with a focus on methodological changes. We also review some of the recent findings from old-growth research in Slovenia and discuss future research needs. The conceptual understanding of how these forests work has slowly evolved, from thinking of them in terms of stable systems to more dynamic and unpredictable ones due to the influence of natural disturbances and indirect human influences. In accordance with this thinking, the methods used to study old-growth forests have changed from descriptions of stand structure to studies that address natural processes and ecosystem functions.


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