scholarly journals Variation in Phytochemical, Antioxidant and Volatile Composition of Pomelo Fruit (Citrus grandis (L.) Osbeck) during Seasonal Growth and Development

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1941
Author(s):  
Arun Kumar Gupta ◽  
Subhamoy Dhua ◽  
Partha Pratim Sahu ◽  
Giulia Abate ◽  
Poonam Mishra ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Different Parts

Citrus fruits exhibit a high level of different phytoconstituents, of which the changes in the different parts of the fruit during ripening have not been thoroughly studied yet. Thus, in this study, we have investigated how different parts of pomelo fruit (Citrus grandis L.) are modified throughout the development of two consecutive growing seasons. In detail, the main phytochemical compounds, such as total phenolic content, total flavonoid content, antioxidant capacity, DPPH free radical scavenging activity, Ferric reducing antioxidant power (FRAP), and naringin and tannin content, were analyzed. A systematic metabolism of these compounds was found during the development of the fruit, but some pomelo tissues showed a fluctuating trend, suggesting a dependence on the different growing season. Focusing on the tissue distribution of these compounds, the fruit membrane contained the highest level of total phenolic and flavonoid content; fruit flavedo displayed the highest antioxidant capacities and FRAP activities, whereas maximum accumulation of naringin was noticed in fruit albedo. Instead, the highest DPPH free radical scavenging activity and tannin contents were found in the pomelo juice. Regarding the distribution of compounds, a possible bias pattern for the accumulation of those compounds has been noticed throughout the fruit development. From the GC-MS analysis, a total of 111 compounds were identified, where 91 compounds were common in both seasons. Overall, these results could be useful for the food processing industry as guidelines for excellent quality foods and for introducing health-beneficial products and components into our daily diets.

scholarly journals Evaluation of Phenolic Content and Free Radical Scavenging Activity of Indonesia Wild Honey Collected from Seven Different Regions

2019 ◽  
Vol 8 (6) ◽  
pp. 94
Author(s):  
Y. Riswahyuli ◽  
Abdul Rohman ◽  
Francis M. C. S. Setyabudi ◽  
Sri Raharjo
Keyword(s):  
Free Radical ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content

Several wild honey samples collected from seven different regions in Indonesia were investigated to determine their total phenolic content, flavonoid content, and free radical scavenging activity by analyzing the 1,1-diphenyl-2-picrylhydrazyl (DDPH) radical and phenolic profile. Rutin, (+)-catechin, ferulic acid, and galangin were found to be the major phenolic compounds of Indonesia wild honey. The total phenolic content significantly correlated with the total flavonoid content (p=0.000) and the percentage of DPPH radical scavenging activity (p=0.000). Results indicated that there are different polyphenol profiles among the different regions.

scholarly journals Phytochemical Screening, Free Radical Scavenging and α-Amylase Inhibitory Activities of Selected Medicinal Plants from Western Nepal

Medicines ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 70 ◽  
Author(s):  
Kusum Sai ◽  
Rashmi Thapa ◽  
Hari Prasad Devkota ◽  
Khem Raj Joshi
Keyword(s):  
Free Radical ◽  
Medicinal Plants ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Inhibitory Activities

Background: More than 700 plants are reported to be used for medicinal purposes in Nepal; however, many of them are not studied for their scientific evidences. The aims of the present study were the estimation of the total phenolic and flavonoid contents, and the evaluation of the free radical scavenging and α-amylase inhibitory activities of five selected medicinal plants from western Nepal: Aeschynanthus parviflorus Wall. (Gesneriaceae), Buddleja asiatica Lour. (Loganiaceae), Carica papaya L. (Caricaceae), Drepanostachyum falcatum (Nees) Keng f. (Gramineae) and Spondias pinnata (L. f.) Kurz (Anacardiaceae). Methods: The total phenolic content (TPC) and total flavonoid content (TFC) were measured using Folin-Ciocalteu’s phenol reagent and aluminium chloride methods, respectively. A 1,1–diphenyl–2–picrylhydrazyl (DPPH) free radical scavenging assay was used to evaluate the free radical scavenging activity and an α-amylase inhibitory assay was carried out to determine the in vitro antidiabetic activity. Results: The phytochemical screening of five hydroalcoholic plant extracts revealed the presence of various secondary metabolites, including alkaloids, flavonoids, reducing sugars, saponins, terpenoids and tannins. The amounts of total phenolics and flavonoids were found to be the highest in B. asiatica leaf extract, which also showed the most potent free radical scavenging activity. Extract of C. papaya fruits showed the highest α-amylase inhibitory activity, whereas the extracts of B. asiatica leaves and S. pinnata leaves exhibited moderate activity. Conclusions: Some of the medicinal plants selected in this study showed high TPC and TFC values and potent bioactivities. These results may provide the scientific evidences of the traditional uses of these plants. However, further detailed studies on bioactive compounds isolation and identification and evaluation of in vivo pharmacological activities should be performed in future.

scholarly journals Pomegranate (Punica granatum L.) Extract and Its Anthocyanin and Copigment Fractions—Free Radical Scavenging Activity and Influence on Cellular Oxidative Stress

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1617
Author(s):  
Tina Kostka ◽  
Johanna Josefine Ostberg-Potthoff ◽  
Karlis Briviba ◽  
Seiichi Matsugo ◽  
Peter Winterhalter ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Plant Metabolites

Secondary plant metabolites, e.g., polyphenols, are widely known as health-improving compounds that occur in natural functional foods such as pomegranates. While extracts generated from these fruits inhibit oxidative stress, the allocation of these effects to the different subgroups of substances, e.g., anthocyanins, “copigments” (polyphenols without anthocyanins), or polymeric compounds, is still unknown. Therefore, in the present study, polyphenols from pomegranate juice were extracted and separated into an anthocyanin and copigment fraction using adsorptive membrane chromatography. Phenolic compounds were determined by high performance liquid chromatography with photodiode array (HPLC–PDA) detection and HPLC-PDA electrospray ionization tandem mass spectrometry (HPLC–PDA–ESI–MS/MS), while the free radical scavenging activity of the pomegranate XAD‑7 extract and its fractions was evaluated by the Trolox equivalent antioxidant capacity (TEAC) assay and electron spin resonance (ESR) spectroscopy. Compared to juice, the total phenolic content and free radical scavenging potential was significantly higher in the pomegranate XAD-7 extract and its fractions. In comparison to the anthocyanin and copigment fraction, pomegranate XAD-7 extract showed the highest radical scavenging activity against galvinoxyl and DPPH radicals. Moreover, the enriched XAD-7 extract and its fractions were able to protect human hepatocellular HepG2 cells against oxidative stress induced by hydrogen peroxide. Overall, these results indicated that anthocyanins and copigments act together in reducing oxidative stress.

scholarly journals FREE RADICAL SCAVENGING ACTIVITY OF MARINE SPONGES COLLECTED FROM KOVALAM, CHENNAI

2017 ◽  
Vol 10 (6) ◽  
pp. 321 ◽  
Author(s):  
Yuvarani Thambidurai ◽  
Sudarsanam D ◽  
Habeeb Skm ◽  
Ashok Kumar T
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Marine Sponges ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Frap Assay ◽  
Antioxidant Power

Objective: The main focus of this study is to screen the marine sponges for potent free radical scavenging activity. Methods: Various methods such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assay are employed to ascertain the antioxidant properties of marine sponges namely Dysidea herbacea and Sigmadocia pumila. Results: On analyzing, the result of ABTS assay D. herbacea and S. pumila exhibited almost equal antioxidant properties. While calculating the inhibitory concentration 50% value for DPPH assay, the Sample 1 and 2 has an IC of 655.49 and 826.739 μl, respectively, and in FRAP assay, the Sample 1 and 2 has an IC of 67.587 and 74.57 μg, respectively. Conclusion: Overall from this assay, D. herbacea revealed slightly better antioxidant activity when compared to S. pumila, also which in future may serve as a better source to fight against various diseases

scholarly journals EVALUATION OF PHYTOCONSTITUENTS, AND ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF JUGLANS REGIA FROM KASHMIR REGION

2019 ◽  
pp. 89-92
Author(s):  
ISHFAQ KHAN ◽  
NEHA CHAUHAN ◽  
CHAUHAN PK ◽  
MOHD. AZHAR KHAN
Keyword(s):  
Free Radical ◽  
Gallic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oral Bacteria ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Standard Curve

Objectives: The main focus of the present study was to evaluate the antimicrobial efficacy (against oral pathogenic bacteria), free radical scavenging activity, and total phenolic and flavonoids content (TPC and TFC) of methanolic extract (ME) of J. regia obtained from Kashmir region. Methods: The plant part was collected and its ME was prepared. ME was subjected to antibacterial activity against oral bacteria such as Staphylococcus aureus, Streptococcus mutans, and Pseudomonas aeruginosa. The free radical scavenging activity was determined using 2,2-diphenyl- 1-picrylhydrazylhydrate (DPPH) assay. TPC and TFC were also determined using a standard curve equation of gallic acid and quercetin. A standard curve using different concentrations of gallic acid and quercetin was drawn from which the concentration of phenols in the test sample was calculated and expressed in mg/g. Results: The ME of J. regia was found effective against all the strains of microorganisms responsible for oral infection understudy. It was also observed that scavenging of DPPH increased with the increase in concentration for both standard ascorbic and methanolic bark extract of J. regia showing its antioxidant potential. The TPC and TFC of ME was found to be 43.35±0.079 and 17.28±0.125. Conclusions: The results obtained from the study clearly indicate that the walnut bark from Kashmir region can be a good candidate for employment as an antibacterial against oral pathogens. J. regia bark was found to be a good source of healthy compounds such as phenolic and flavonoids, suggesting that its bark could be useful to prevent diseases in which free radicals are present.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (8) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
iham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel source of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels reveled a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

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