scholarly journals Identification of Bacterial Wilt (Erwinia tracheiphila) Resistances in USDA Melon Collection

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1972
Author(s):  
Bimala Acharya ◽  
Lucas Mackasmiel ◽  
Ali Taheri ◽  
Christine A. Ondzighi-Assoume ◽  
Yiqun Weng ◽  
...  

Bacterial wilt (BW) caused by the Gram-negative bacterium, Erwinia tracheiphila (Et.), is an important disease in melon (Cucumis melo L.). BW-resistant commercial melon varieties are not widely available. There are also no effective pathogen-based disease management strategies as BW-infected plants ultimately die. The purpose of this study is to identify BW-resistant melon accessions in the United States Department of Agriculture (USDA) collection. We tested 118 melon accessions in two inoculation trials under controlled environments. Four-week-old seedlings of test materials were mechanically inoculated with the fluorescently (GFP) labeled or unlabeled E. tracheiphila strain, Hca1-5N. We recorded the number of days to wilting of inoculated leaf (DWIL), days to wilting of whole plant (DWWP) and days to death of the plant (DDP). We identified four melon lines with high resistance to BW inoculation based on all three parameters. Fluorescent microscopy was used to visualize the host colonization dynamics of labeled bacteria from the point of inoculation into petioles, stem and roots in resistant and susceptible melon accessions, which provides an insight into possible mechanisms of BW resistance in melon. The resistant melon lines identified from this study could be valuable resistance sources for breeding of BW resistance as well as the study of cucurbit—E. tracheiphila interactions.

1993 ◽  
Vol 28 (3-5) ◽  
pp. 65-68
Author(s):  
Michelle Miller

The following case study addresses the difficulties and promise of developing a statewide interagency public information campaign to raise general awareness of water quality issues and governmental programs to address them. Due to only moderate success of voluntary programs to curb nonpoint source pollution, agencies are looking toward information and education programs to motivate the public toward conservation behavior. One of the biggest obstacles in developing an effective information/education program is institutional barriers to interagency cooperation, mirroring difficulties local conservationists encounter in their work to restore and maintain water quality at the watershed level. Cooperation between federal agencies, and resource commitment to public information is necessary at the federal level, as well as state and local levels. Agencies involved to date include the United States Department of Agriculture-Soil Conservation Service; Wisconsin State Departments of Natural Resources, and Agriculture, Trade and Consumer Protection and Administration; University of Wisconsin-Extension; Wisconsin Land Conservation Association.


2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Brittany L Schappach ◽  
Rayda K Krell ◽  
Victoria L Hornbostel ◽  
Neeta P Connally

Abstract The Asian longhorned tick (ALT), Haemaphysalis longicornis Neumann (Acari: Ixodidae), is a three-host tick that was first detected outside of United States Department of Agriculture (USDA) quarantine in Hunterdon County, New Jersey, in 2017 and subsequently found in another 14 states. In its native Asia, and where it has become established in Australia and New Zealand, ALTs feed on a variety of hosts and are economically important livestock pests and competent vectors of multiple pathogens to humans and other animals. The degree to which ALT will become a persistent livestock pest or competent vector for introduced or existing pathogens in the United States is yet unclear. Because of its vast host availability, ability to reproduce asexually, known vector competence, and the presence of multiple life stages on hosts, the expansion of ALT establishment in the United States is expected, and is a significant public health and veterinary concern. In this paper, we discuss the biology, geographical distribution, life cycle and seasonal activity, reproduction, identification, medical and veterinary implications, management options, and future concerns in the United States.


2008 ◽  
Vol 21 (suppl) ◽  
pp. 27s-37s ◽  
Author(s):  
Hugo Melgar-Quinonez ◽  
Michelle Hackett

Measuring household food insecurity represents a challenge due to the complexity and wide array of factors associated with this phenomenon. For over one decade, researchers and agencies throughout the world have been using and assessing the validity of variations of the United States Department of Agriculture Household Food Security Supplemental Module. Thanks to numerous studies of diverse design, size, and purpose, the Household Food Security Supplemental Module has shown its suitability to directly evaluate the perceptions of individuals on their food security status. In addition, challenges and limitations are becoming clearer and new research questions are emerging as the process advances. The purpose of this article is to describe the development, validation procedures, and use of the Household Food Security Supplemental Module in very diverse settings. The most common Household Food Security Supplemental Module related studies have been conducted using criterion validity, Rasch modeling and Cronbach-Alpha Coefficient. It is critical that researchers, policy makers, governmental and non-governmental agencies intensify their efforts to further develop tools that provide valid and reliable measures of food security in diverse population groups. Additional work is needed to synthesize a universally applicable tool able to capture the global human phenomenon of food insecurity.


2017 ◽  
Vol 32 (3) ◽  
Author(s):  
Karl L Larson ◽  
Megan Mullaney ◽  
Esther Mwangi ◽  
Deu Xiong ◽  
Frances Ziegler

Food deserts are identified by the United States Department of Agriculture as areas where asignificant proportion of the population lack access to quality food choices. The purpose of this study was toassess the availability of quality fruits, vegetables, dry good, and meats at corner store locations in a south-central Minnesota county designated a food desert. Corner stores (n=22) were audited for the presence of20 fresh fruits and vegetables, low-fat meats, and whole grain dry goods. Results indicate more than three-quarters of corner stores did not provide sufficient access to healthy foods, and were not provide sufficientaccess to quality food for residents in the county.


Foods ◽  
2018 ◽  
Vol 7 (8) ◽  
pp. 126 ◽  
Author(s):  
Edgar Chambers ◽  
Sandria Godwin ◽  
Taylor Terry

Research has shown that consumers use unsafe food handling practices when preparing poultry, which can increase the risk of foodborne illness such as salmonellosis or campylobacteriosis. Recipes from cookbooks, magazines, and the internet commonly are used as sources for consumers to prepare food in homes and the expectation is that food will be safe when prepared. According to the United States Department of Agriculture (USDA), using a thermometer properly is the only way to accurately check for doneness of poultry. The objective of this study was to assess poultry recipes, including recipes for whole birds and poultry parts, to determine if food safety information concerning thermometer use was included within the recipe. Poultry recipes (n = 474) were collected from 217 cookbooks, 28 magazines, 59 websites, and seven blogs. Approximately 33.5% of the recipes contained a specific temperature for doneness, with 73% of those cooked to ≥165 °F/74 °C, as recommended by USDA. Ninety-four percent of recipes used cooking time and about half of the recipes used visual measurements, such as color or juices running clear, to determine doneness. This study showed that most recipes do not contain appropriate information to assure safe cooking of poultry by consumers. Modifying recipes by adding food safety information, such as thermometer use and proper temperatures, could increase the use of proper food preparation behaviors by consumers.


Sign in / Sign up

Export Citation Format

Share Document