scholarly journals Identification and Expression Analysis of Cold Shock Protein 3 (BcCSP3) in Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis)

Plants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 890 ◽  
Author(s):  
Feiyi Huang ◽  
Jin Wang ◽  
Weike Duan ◽  
Xilin Hou
Keyword(s):  
Cold Stress ◽  
Expression Analysis ◽  
Chinese Cabbage ◽  
Cold Shock ◽  
Quantitative Polymerase Chain Reaction ◽  
Expression Level ◽  
Cold Shock Protein ◽  
Multiple Sequence ◽  
Reading Frame ◽  
Heading Chinese Cabbage

A cold-related protein, cold shock protein 3 (BcCSP3), was isolated from non-heading Chinese cabbage in this study. BcCSP3 can encode 205 amino acids (aa) with an open reading frame (ORF) of 618 base pairs (bp). Multiple sequence alignment and phylogenetic tree analyses showed that BcCSP3 contains an N-terminal cold shock domain and is highly similar to AtCSP2, their kinship is recent. Real-time quantitative polymerase chain reaction (RT-qPCR) showed that the expression level of BcCSP3 in stems and leaves is higher than that in roots. Compared with other stress treatments, the change in BcCSP3 expression level was most pronounced under cold stress. In addition, a BcCSP3–GFP fusion protein was localized to the nucleus and cytoplasm. These results indicated that BcCSP3 may play an important role in response to cold stress in non-heading Chinese cabbage. This work may provide a reference for the identification and expression analysis of other CSP genes in non-heading Chinese cabbage.

A putative cold shock protein-encoding gene isolated from Arthrobacter sp. A2-5 confers cold stress tolerance in yeast and plants

2014 ◽  
Vol 57 (6) ◽  
pp. 775-782 ◽  
Author(s):  
Seong-Kon Lee ◽  
Sung-Han Park ◽  
Jeong-Won Lee ◽  
Hae-Min Lim ◽  
Sun-Young Jung ◽  
...  
Keyword(s):  
Cold Stress ◽  
Stress Tolerance ◽  
Cold Shock ◽  
Cold Shock Protein ◽  
Protein Encoding ◽  
Encoding Gene

Cloning and Expression Analysis of Anthocyanidin Synthase Gene BrcANS from Purple Non-heading Chinese Cabbage

2016 ◽  
Vol 42 (6) ◽  
pp. 850 ◽  
Author(s):  
Yu-Chao XU ◽  
Xi-Lin HOU ◽  
Wei-Wei XU ◽  
Lu-Lu SHEN ◽  
Shi-Lin ZHANG ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Chinese Cabbage ◽  
Cloning And Expression ◽  
Anthocyanidin Synthase ◽  
Heading Chinese Cabbage

scholarly journals Expression analysis of self-incompatibility-associated genes in non-heading Chinese cabbage

2014 ◽  
Vol 13 (3) ◽  
pp. 5025-5035 ◽  
Author(s):  
L. Wang ◽  
C. Wang ◽  
T.T. Ge ◽  
J.J. Wang ◽  
T.K. Liu ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Chinese Cabbage ◽  
Self Incompatibility ◽  
Heading Chinese Cabbage

cDNA Clones and Expression Analysis of cpHSC70 and mtHSC70 in Non-Heading Chinese Cabbage

2013 ◽  
Vol 32 (2) ◽  
pp. 531-540 ◽  
Author(s):  
Hongbing Song ◽  
Xiaoming Song ◽  
Huanhuan Liu ◽  
Tongkun Liu ◽  
Ying Li ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Chinese Cabbage ◽  
Cdna Clones ◽  
Heading Chinese Cabbage

scholarly journals MicroRNAs in the response of non-heading Chinese cabbage to Hyaloperonospora parasitica (downy mildew) infection

2019 ◽  
Author(s):  
Chengzhen Sun ◽  
Die Hu ◽  
Yuan Wang ◽  
Huawei Tan ◽  
Tongkun Liu ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Chinese Cabbage ◽  
Target Genes ◽  
Quantitative Polymerase Chain Reaction ◽  
Callose Deposition ◽  
Hyaloperonospora Parasitica ◽  
Defense Related Gene ◽  
Indole Glucosinolate ◽  
Polymerase Chain ◽  
Heading Chinese Cabbage

Abstract Background MicroRNAs (miRNAs) are a subset of endogenous small non-coding ~21 nucleotide RNAs, which are playing important roles in development, abiotic stress, and pathogen responses. Downy mildew, caused by Hyaloperonospora parasitica , is a severe fungal disease in non-heading Chinese cabbage ( Brassica rapa ssp. chinensis ). However, miRNAs and their targets involved in the response of non-heading Chinese cabbage to downy mildew were still unclear. Results Here, fifteen small RNA libraries were generated from non-heading Chinese cabbage leaves at different time points after H. parasitica inoculation. By deep sequencing, we identified 197 known miRNAs and 112 novel miRNAs. According to data analysis, 8 known miRNAs and 8 novel miRNAs were found to be responsive to downy mildew infection in non-heading Chinese cabbage. Expression levels of 12 predicted miRNAs target genes were determined by reverse transcription-quantitative polymerase chain reaction (qRT-PCR). In this study, we proposed a regulatory network showing that non-heading Chinese cabbage defense response to downy mildew by repressing cell wall pectin, lipid and cellulose catabolic process ( PME12 , PME17 , PLAIVA and GDPDL4 ) and callose deposition ( CYP79B2 ), repressing indole glucosinolate metabolic process ( CYP81F3 ), repressing respiratory burst ( PUB24 ), repressing programmed cell death ( CRK5 ), and inducing defense related gene expression ( ROS1 , LECRK-VII.2 , BAK7 and WRKY48 ). Conclusions This research identified miRNAs associated with downy mildew infection in non-heading Chinese cabbage. Target genes and network analysis contributed to understanding the interaction between plant and pathogen.

scholarly journals Novel Zinc Finger-Homeodomain Gene from Barley (HvZFHD1) is Differentially Regulated During Spike Development and under Hormonal Treatments and Abiotic Stresses

2017 ◽  
Vol 45 (1) ◽  
pp. 89-96
Author(s):  
Saeid ABU-ROMMAN ◽  
Khaldoun AL-HADID
Keyword(s):  
Zinc Finger ◽  
Abiotic Stresses ◽  
Expression Patterns ◽  
Expression Level ◽  
Plant Responses ◽  
Multiple Sequence ◽  
Reading Frame ◽  
C Terminus ◽  
Spike Development ◽  
Putative Zinc Finger

Plant zinc finger-homeodomains (ZFHDs) are transcriptional factors that play an important role in regulating plant growth and development. Several ZFHD genes were cloned and characterized in many plant species. In the present study, a full-length cDNA sequence of ZFHD gene was cloned from barley (termed as HvZFHD1) using reverse transcription polymerase chain reaction (RT-PCR). The sequence analysis showed that the HvZFHD1 was 1477 bp in length, and contained a complete open reading frame (ORF) of 1161 bp. The deduced protein is composed of 386 amino acids, with a predicted molecular weight of 40.46 kDa and a theoretical isoelectric point of 8.5. Multiple sequence alignment indicated that HvZFHD1 protein shared high identity with ZFHD proteins from wheat, maize, and rice. The predicted HvZFHD1 protein contained the characteristic putative zinc finger domain in the N-terminus and a DNA binding homeodomain in the C-terminus. The expression level of HvZFHD1 was investigated using qRT-PCR during spike development and in response to exogenous phytohormones and abiotic stresses. The results showed that the expression level of HvZFHD1 was fluctuated during spike development with higher expression during anthesis, medium milk, late milk, and early dough stages. The expression of barley ZFHD1 was strongly responsive to abscisic acid treatment and was up-regulated in seedlings treated with methyl jasmonate, salicylic acid, and ethephone. In addition, the expression levels of HvZFHD1 were increased by dehydration, salinity, and heat stress, but not affected by cold stress. The expression patterns of HvZFHD1 suggest that it might play a role in flowering and flower development and is involved in plant responses to abiotic stresses.

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