Synthesis and Characterization of a Novel Green Cationic Polyfluorene and Its Potential Use as a Fluorescent Membrane Probe

In the present work, we have synthesized a novel green-emitter conjugated polyelectrolyte Copoly-{[9,9-bis(6′-N,N,N-trimethylammonium)hexyl]-2,7-(fluorene)-alt-4,7-(2-(phenyl) benzo[d] [1,2,3] triazole)} bromide (HTMA-PFBT) by microwave-assisted Suzuki coupling reaction. Its fluorescent properties have been studied in aqueous media and in presence of model membranes of different composition, in order to explore its ability to be used as a green fluorescent membrane probe. The polyelectrolyte was bound with high affinity to the membrane surface, where it exhibited high fluorescence efficiency and stability. HTMA-PFBT showed lower affinity to zwitterionic membranes as compared to anionic ones, as well as a more external location, near the membrane-aqueous interface. Fluorescence microscopy studies confirmed the interaction of HTMA-PFBT with the model membranes, labelling the lipid bilayer without perturbing its morphology and showing a clear preference towards anionic systems. In addition, the polyelectrolyte was able to label the membrane of bacteria and living mammalian cells, separately. Finally, we explored if the polyelectrolyte can function also as a sensitive probe able of detecting lipid-phase transitions. All these results suggest the potential use of HTMA-PFBT as a green membrane marker for bioimaging and selective recognition of bacteria cell over mammalian ones and as a tool to monitor changes in physical state of lipid membranes.

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Palladium Supported on Oleic Acid-coated Magnetic Nanoparticle as an Efficient Catalyst for Heck Coupling Reaction in Aqueous Media

Letters in Organic Chemistry ◽

10.2174/1570178611666140815214322 ◽

2014 ◽

Vol 11 (10) ◽

pp. 707-712 ◽

Cited By ~ 1

Author(s):

Ezzat Rafiee ◽

Ali Ataei ◽

Mohammad Joshaghani

Keyword(s):

Oleic Acid ◽

Magnetic Nanoparticle ◽

Aqueous Media ◽

Coupling Reaction ◽

Heck Coupling ◽

Efficient Catalyst

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Polylactide-based stent coatings: biodegradable polymeric coatings capable of maintaining sustained release of the thrombolytic enzyme streptokinase

Pure and Applied Chemistry ◽

10.1515/pac-2019-1101 ◽

2020 ◽

Vol 92 (8) ◽

pp. 1329-1340

Author(s):

A. G. Kolmakov ◽

A. S. Baikin ◽

S. V. Gudkov ◽

K. N. Belosludtsev ◽

E. O. Nasakina ◽

...

Keyword(s):

Mechanical Properties ◽

Polymer Films ◽

De Novo ◽

Membrane Surface ◽

Aqueous Media ◽

Polymeric Coatings ◽

Short Term ◽

Thrombolytic Activity ◽

Viable Cells ◽

Nitinol Stents

AbstractThe paper describes synthesis and testing of novel biodegradable polylactide-based polymer membranes with desired mechanical properties, which are capable of sustained and directed release of biomacromolecules with high molecular weight (in particular, streptokinase; m.w. 47 kDa). Streptokinase is a pharmaceutical agent, possessing a pronounced thrombolytic activity. The membranes synthesized had a percentage elongation of 2–11% and tensile strength of 25–85 MPa. They were biodegradable – yet being stored in aqueous media in the absence of biological objects, would be dissolved by no more than 10% in 6 months. The synthesized membranes were capable of controlled release of streptokinase into the intercellular space, with the enzyme retaining more than 90% of its initial activity. The rate of streptokinase release from the membranes varied from 0.01 to 0.04 mg/day per cm2 of membrane surface. The membrane samples tested in the work did not have any short-term toxic effects on the cells growing de novo on the membrane surface. The mitotic index of those cells was approximately 1.5%, and the number of non-viable cells on the surface of the polymer films did not exceed 3–4% of their total amount. The implantation of the synthesized polymers – as both individual films and coatings of nitinol stents – was not accompanied by any postoperative complications. The subsequent histological examination revealed no abnormalities. Two months after the implantation of polymer films, only traces of polylactide were found in the implant-surrounding tissues. The implantation of stents coated with streptokinase-containing polymers resulted in the formation of a mature and thick connective-tissue capsules. Thus, the polylactide membranes synthesized and tested in this work are biodegradable, possess the necessary mechanical properties and are capable of sustained and directed release of streptokinase macromolecules.

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ChemInform Abstract: First Cross-Coupling Reaction of Potassium Aryltrifluoroborates with Organic Chlorides in Aqueous Media Catalyzed by an Oxime-Derived Palladacycle.

ChemInform ◽

10.1002/chin.200913083 ◽

2009 ◽

Vol 40 (13) ◽

Author(s):

Emilio Alacid ◽

Carmen Najera

Keyword(s):

Aqueous Media ◽

Coupling Reaction ◽

Cross Coupling ◽

Cross Coupling Reaction

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The Use of Bacteriophage for Detection and Biocontrol of Foodborne Pathogen

Indonesian Bulletin of Animal and Veterinary Sciences ◽

10.14334/wartazoa.v28i1.1791 ◽

2018 ◽

Vol 28 (1) ◽

pp. 33

Author(s):

Tati Ariyanti

Keyword(s):

Mammalian Cells ◽

Food Industry ◽

Pathogenic Bacteria ◽

Foodborne Pathogen ◽

Foodborne Disease ◽

Bacterial Cells ◽

Detection Tool ◽

Specific Receptors ◽

Antibiotics Detection ◽

Potential Use

Bacteriophages are viruses that have ability to attack bacterial cells in specific receptors, infect, multiply in bacterial cells and eventually lyse bacterial cells. This unique bacteriophage character is highly beneficial because it is harmless to mammalian cells and does not interfere with natural microbes. Bacteriophages are easy to obtain because they are widespread in the environment such as soil, water, animal, and farm waste or food. This paper describes the potential use of bacteriophages to detect pathogen and foodborne pathogen biocontrol. Bacteriophages are very potential to control the growth of pathogenic bacteria both in food industry and environment. Bacteriophages act as antibiotics, detection tool for pathogenic bacteria in the food chain, food biopreservative from pathogen bacteria contamination, and foodborne disease prevention. Although research on bacteriophage in Indonesia has not been widely reported, research on bacteriophage utilization is being carried on.

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Reduction of Biofouling of a Microfiltration Membrane Using Amide Functionalities—Hydrophilization without Changes in Morphology

Polymers ◽

10.3390/polym12061379 ◽

2020 ◽

Vol 12 (6) ◽

pp. 1379

Author(s):

Daniel Breite ◽

Marco Went ◽

Andrea Prager ◽

Mathias Kühnert ◽

Agnes Schulze

Keyword(s):

Membrane Fouling ◽

Average Pore Size ◽

Membrane Surface ◽

Aqueous Media ◽

Soxhlet Extraction ◽

Hydrophobic Membrane ◽

Average Pore ◽

Water Contact ◽

Microfiltration Membrane ◽

Membrane Performance

A major goal of membrane science is the improvement of the membrane performance and the reduction of fouling effects, which occur during most aqueous filtration applications. Increasing the surface hydrophilicity can improve the membrane performance (in case of aqueous media) and decelerates membrane fouling. In this study, a PES microfiltration membrane (14,600 L m−2 h−1 bar−1) was hydrophilized using a hydrophilic surface coating based on amide functionalities, converting the hydrophobic membrane surface (water contact angle, WCA: ~90°) into an extremely hydrophilic one (WCA: ~30°). The amide layer was created by first immobilizing piperazine to the membrane surface via electron beam irradiation. Subsequently, a reaction with 1,3,5-benzenetricarbonyl trichloride (TMC) was applied to generate an amide structure. The presented approach resulted in a hydrophilic membrane surface, while maintaining permeance of the membrane without pore blocking. All membranes were investigated regarding their permeance, porosity, average pore size, morphology (SEM), chemical composition (XPS), and wettability. Soxhlet extraction was carried out to demonstrate the stability of the applied coating. The improvement of the modified membranes was demonstrated using dead-end filtration of algae solutions. After three fouling cycles, about 60% of the initial permeance remain for the modified membranes, while only ~25% remain for the reference.

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Highly stable noble metal nanoparticles dispersible in biocompatible solvents: synthesis of cationic phosphonium gold nanoparticles in water and DMSO

Faraday Discussions ◽

10.1039/c5fd00131e ◽

2016 ◽

Vol 186 ◽

pp. 77-93 ◽

Cited By ~ 4

Author(s):

Yon Ju-Nam ◽

Wanisa Abdussalam-Mohammed ◽

Jesus J. Ojeda

Keyword(s):

Gold Nanoparticles ◽

Drug Carrier ◽

Aqueous Media ◽

Noble Metal Nanoparticles ◽

Great Opportunity ◽

Thiolate Ligands ◽

New Family ◽

Uv Visible ◽

Potential Use

In this work, we report the synthesis of novel cationic phosphonium gold nanoparticles dispersible in water and dimethyl sulfoxide (DMSO) for their potential use in biomedical applications. All the cationic-functionalising ligands currently reported in the literature are ammonium-based species. Here, the synthesis and characterisation of an alternative system, based on phosphonioalkylthiosulfate zwitterions and phosphonioalkylthioacetate were carried out. We have also demonstrated that our phosphonioalkylthiosulfate zwitterions readily disproportionate into phosphonioalkylthiolates in situ during the synthesis of gold nanoparticles produced by the borohydride reduction of gold(iii) salts. The synthesis of the cationic gold nanoparticles using these phosphonium ligands was carried out in water and DMSO. UV-visible spectroscopic and TEM studies have shown that the phosphonioalkylthiolates bind to the surface of gold nanoparticles which are typically around 10 nm in diameter. The resulting cationic-functionalised gold nanoparticles are dispersible in aqueous media and in DMSO, which is the only organic solvent approved by the U.S. Food and Drug Administration (FDA) for drug carrier tests. This indicates their potential future use in biological applications. This work shows the synthesis of a new family of phosphonium-based ligands, which behave as cationic masked thiolate ligands in the functionalisation of gold nanoparticles. These highly stable colloidal cationic phosphonium gold nanoparticles dispersed in water and DMSO can offer a great opportunity for the design of novel biorecognition and drug delivery systems.

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Palladium-Schiff-base-silica framework as a robust and recyclable catalyst for Suzuki–Miyaura cross-coupling in aqueous media

RSC Advances ◽

10.1039/c5ra01574j ◽

2015 ◽

Vol 5 (48) ◽

pp. 38085-38092 ◽

Cited By ~ 16

Author(s):

Tahshina Begum ◽

Manoj Mondal ◽

Pradip K. Gogoi ◽

Utpal Bora

Keyword(s):

Schiff Base ◽

Catalytic Activity ◽

Room Temperature ◽

Aqueous Media ◽

Coupling Reaction ◽

Cross Coupling ◽

Recyclable Catalyst ◽

Cross Coupling Reaction

A novel Pd@imine-SiO2 catalyst was prepared and found to exhibit excellent catalytic activity in a Suzuki-Miyaura cross-coupling reaction under aqueous media at room temperature.

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Magneto-Fluorescent Hybrid Sensor CaCO3-Fe3O4-AgInS2/ZnS for the Detection of Heavy Metal Ions in Aqueous Media

Materials ◽

10.3390/ma13194373 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4373

Author(s):

Danil Kurshanov ◽

Pavel Khavlyuk ◽

Mihail Baranov ◽

Aliaksei Dubavik ◽

Andrei Rybin ◽

...

Keyword(s):

Heavy Metal ◽

Metal Ions ◽

Heavy Metal Ions ◽

Aqueous Media ◽

Semiconductor Quantum Dots ◽

Fluorescent Properties ◽

Fluorescence Sensors ◽

The Past ◽

Porous Calcium Carbonate ◽

Toxic Ions

Heavy metal ions are not subject to biodegradation and could cause the environmental pollution of natural resources and water. Many of the heavy metals are highly toxic and dangerous to human health, even at a minimum amount. This work considered an optical method for detecting heavy metal ions using colloidal luminescent semiconductor quantum dots (QDs). Over the past decade, QDs have been used in the development of sensitive fluorescence sensors for ions of heavy metal. In this work, we combined the fluorescent properties of AgInS2/ZnS ternary QDs and the magnetism of superparamagnetic Fe3O4 nanoparticles embedded in a matrix of porous calcium carbonate microspheres for the detection of toxic ions of heavy metal: Co2+, Ni2+, and Pb2+. We demonstrate a relationship between the level of quenching of the photoluminescence of sensors under exposure to the heavy metal ions and the concentration of these ions, allowing their detection in aqueous solutions at concentrations of Co2+, Ni2+, and Pb2+ as low as ≈0.01 ppm, ≈0.1 ppm, and ≈0.01 ppm, respectively. It also has importance for application of the ability to concentrate and extract the sensor with analytes from the solution using a magnetic field.

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