scholarly journals Pretreatments of Non-Woody Cellulosic Feedstocks for Bacterial Cellulose Synthesis

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1645 ◽  
Author(s):  
Ekaterina I. Kashcheyeva ◽  
Yulia A. Gismatulina ◽  
Vera V. Budaeva
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bacterial Cellulose ◽  
High Performance ◽  
Combined Treatment ◽  
Single Stage ◽  
High Tech ◽  
Cellulose Synthesis ◽  
Oat Hulls ◽  
Naoh Solution ◽  
Hydrolysis Of

Pretreatment of biomass is a key step in the production of valuable products, including high-tech bacterial cellulose. The efficiency of five different pretreatment methods of Miscanthus and oat hulls for enzymatic hydrolysis and subsequent synthesis of bacterial cellulose (BC) was evaluated herein: Hydrothermobaric treatment, single-stage treatments with dilute HNO3 or dilute NaOH solution, and two-stage combined treatment with dilute HNO3 and NaOH solutions in direct and reverse order. The performance of enzymatic hydrolysis of pretreatment products was found to increase by a factor of 4−7. All the resultant hydrolyzates were composed chiefly of glucose, as the xylose percentage in total reducing sugars (RS) was 1−9%. The test synthesis of BC demonstrated good quality of nutrient media prepared from all the enzymatic hydrolyzates, except the hydrothermobaric treatment hydrolyzate. For biosynthesis of BC, single-stage pretreatments with either dilute HNO3 or dilute NaOH are advised due their simplicity and the high performance of enzymatic hydrolysis of pretreatment products (RS yield 79.7−83.4%).

Enzymatic hydrolysis of celluloses obtained via the hydrothermal processing of Miscanthus and oat hulls

2014 ◽  
Vol 6 (1) ◽  
pp. 67-71 ◽  
Author(s):  
E. I. Makarova ◽  
V. V. Budaeva ◽  
E. A. Skiba ◽  
G. V. Sakovich
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrothermal Processing ◽  
Oat Hulls ◽  
Hydrolysis Of

scholarly journals Enzymatic hydrolysis of bacterial cellulose in the presence of a non‐catalytic cerato‐platanin protein

2021 ◽  
pp. 51886
Author(s):  
Cesare Rovera ◽  
Simone Luti ◽  
Luigia Pazzagli ◽  
Ellen L. Heeley ◽  
Chaoying Wan ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bacterial Cellulose ◽  
Hydrolysis Of

scholarly journals Towards Sustainable High‐Performance Thermoplastics: Synthesis, Characterization, and Enzymatic Hydrolysis of Bisguaiacol‐Based Polyesters

ChemSusChem ◽  
2018 ◽  
Vol 11 (15) ◽  
pp. 2468-2468
Author(s):  
Silvio Curia ◽  
Antonino Biundo ◽  
Isabel Fischer ◽  
Verena Braunschmid ◽  
Georg M. Gübitz ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
High Performance ◽  
Hydrolysis Of

scholarly journals Study of Pretreatment and Enzymatic Hydrolysis on Microcrystalline Cellulose and HVS A4 Paper in Pressurized CO2 Media

2015 ◽  
Vol 9 (7) ◽  
pp. 16 ◽  
Author(s):  
Ian Eka Pramudita ◽  
Marcella Lauditta Noviana ◽  
Henky Muljana
Keyword(s):  
High Performance Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
Microcrystalline Cellulose ◽  
Levulinic Acid ◽  
High Performance ◽  
Model Compound ◽  
Bioethanol Production ◽  
Fixed Temperature ◽  
Pre Treatment ◽  
Hydrolysis Of

The aim of this work is to investigate the potential used of pressurized CO2 in the pre-treatment and the enzymatic hydrolysis of waste HVS A4 paperto produce a monomeric sugars (C5 and C6 sugars) which is a precursor for bioethanol production. Prior to the utilization of waste HVS A4 paper, the microcrystalline cellulose and HVS A4 paper were first used in the experiments as model compound in order to gain better insights of the process. The experiment consists of two main parts which are the preliminary experimentsto determine the best pretreatment conditionsbetween two selected pressure values (80 and 100 bar) at a fixed temperature (T = 75 oC) and the enzymatic hydrolysis experiments. In the latter, the microcrystalline and HVS A4 paper were hydrolyzed at different pressures (100 bar, 125 bar, and 150 bar) and at different cellulase intakes (1% (v/v), 3% (v/v), and 5% (v/v)) with a fixed temperature (50°C). The hydrolyzed products were analyzed with a High Performance Liquid Chromatography (HPLC) to quantify the monomeric sugars and to determine the presence of the side products (furfural, HMF and levulinic acid). Within the experimental range, a maximum glucose concentration of 7602.35 ppm and 4560.79 ppm are obtained for microcrystalline and HVS A4 paper, respectively. In addition, there are no furfural, HMF and levulinic acid detected in the products This study shows a potential used of pressurized CO2 in the pretreatment and enzymatic hydrolysis of the model compound and gives a better insight for further application.

The influence of urea and formaldehyde on enzymatic hydrolysis of cellulose

2020 ◽  
Vol 110 ◽  
pp. 92-96
Author(s):  
Monika Marchwicka ◽  
Anna Lesiak ◽  
Andrzej Radomski
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Enzymatic Hydrolysis ◽  
High Performance ◽  
Glucose Yield ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Effect of selected urea and formaldehyde concentrations on glucose yield of enzymatic hydrolysis of cellulose was investigated. Urea and formaldehyde were added separately at the concentrations of 0.001, 0.002 and 0.005 g/cm3. Glucose was determined by high-performance liquid chromatography (HPLC). It was found that the used concentrations of urea didn’t influence glucose yield. In the case of formaldehyde, the results vary between used concentrations. The glucose yield of enzymatic hydrolysis of cellulose with the highest investigated concentration of formaldehyde (0.005 g/cm3) decreased by 50 %.

Enzymatic hydrolysis of biomimetic bacterial cellulose–hemicellulose composites

2018 ◽  
Vol 190 ◽  
pp. 95-102 ◽  
Author(s):  
Paavo A. Penttilä ◽  
Tomoya Imai ◽  
Jarl Hemming ◽  
Stefan Willför ◽  
Junji Sugiyama
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bacterial Cellulose ◽  
Hydrolysis Of

Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover

2021 ◽  
Vol 115 ◽  
pp. 29-36
Author(s):  
Florentyna Akus-Szylberg ◽  
Andrzej Antczak ◽  
Janusz Zawadzki
Keyword(s):  
Chemical Composition ◽  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
High Performance ◽  
Aqueous Ammonia ◽  
Ammonia Solution ◽  
Raw Material ◽  
Different Temperatures ◽  
Ammonia Pretreatment ◽  
Hydrolysis Of

Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover. The aim of this research was to investigate the effect of applying two different temperatures of the soaking aqueous ammonia treatment on the chemical composition and enzymatic hydrolysis yield of the corn stover. Native corn stover as well as solid fractions after 20 h of alkali pretreatment performed at 15% ammonia solution and at 50 °C or 90 °C were analysed in terms of cellulose, holocellulose, lignin and extractives content. Both untreated and treated samples were subjected to the enzymatic hydrolysis and hydrolysates were examined with a high performance liquid chromatography (HPLC). Results indicated a significant development of enzymatic digestibility of the SAA treated biomass. Furthermore, a 38.7% and a 68.9% delignification levels in the biomass treated with ammonia at respectively 50 °C and 90 °C process comparing to the raw material were achieved.

MAIN TECHNOLOGICAL STAGES OF BACTERIAL CELLULOSE SYNTHESIS FROM EASILY RENEWABLE CELLULOSIC FEEDSTOCKS BY МEDUSOMYCES GISEVII SA-12 SYMBIOTIC CULTURE

2020 ◽  
pp. 290-292
Author(s):  
V. Budaeva ◽  
Е. Gladysheva ◽  
N. Shavyrkina ◽  
I. Pavlov ◽  
D. Golubev ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Synthesis ◽  
High Quality ◽  
Bacterial Nanocellulose ◽  
Oat Hulls ◽  
Pretreatment Method

A technology has been developed for the synthesis of bacterial nanocellulose from oat hulls and Miscanthus. The Мedusomyces gisevii Sa-12 microbial producer was found to synthesize bacterial nanocellulose of high quality, regardless of the feedstock type and pretreatment method thereof.

Enzymatic hydrolysis of the products of hydro-thermobaric processing of Miscanthus and oat hulls

2013 ◽  
Vol 5 (4) ◽  
pp. 335-341 ◽  
Author(s):  
V. V. Budaeva ◽  
E. I. Makarova ◽  
E. A. Skiba ◽  
G. V. Sakovich
Keyword(s):  
Enzymatic Hydrolysis ◽  
Oat Hulls ◽  
Hydrolysis Of

scholarly journals The effect of phytohormones on the growth, cellulose production and pellicle properties of Gluconacetobacter xylinus ATCC 53582

2013 ◽  
Vol 2 (1s) ◽  
pp. 7 ◽  
Author(s):  
Osama Qureshi ◽  
Hira Sohail ◽  
Andrew Latos ◽  
Janice L. Strap
Keyword(s):  
Bacterial Cellulose ◽  
High Performance ◽  
Crystallinity Index ◽  
Cellulose Synthesis ◽  
Gluconacetobacter Xylinus ◽  
Cellulose Production ◽  
Endogenous Production ◽  
Microbe Interactions ◽  
Wet Weight ◽  
Culture Supernatants

<em>Gluconacetobacter xylinus</em> is a plant-associated bacterium best studied for its cellulose production. Bacterial cellulose is important in facilitating plant-microbe interactions but little is known about the effect that exogenous phytohormones have on bacterial cellulose synthesis or the growth of <em>G. xylinus</em>. We characterized the growth, development and effect on pellicle characteristics caused by exogenous indole-3- acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and zeatin (Z) over a range of concentrations (1 nM to 100 &mu;M). These phytohormones are plant growth regulators known to be involved plant development including fruit ripening and stress tolerance. Each of these hormones stimulated <em>G. xylinus</em> growth and influenced its pellicle characteristics. Exogenous IAA had the greatest effect on <em>G. xylinus</em> pellicles. Growth in IAA produced thin pellicles with very little cellulose. In general, pellicle wet weight was inversely proportional to the bacterial cellulose yield when cultures were grown in the presence of ABA, suggesting ABA influenced pellicle density and hydration. The crystallinity index, CI (IR) of cellulose produced in the presence of each phytohormone over a variety of concentrations was determined by Fourier transform infrared spectroscopy. The observed effect on cellulose crystallinity was concentration and hormone dependent. GA caused the greatest alterations in crystallinity with the highest CI (IR)=0.94 at 1 &mu;M and the lowest CI (IR)=0.47 at 500 nM. Endogenous production of hormones by <em>G. xylinus</em> was investigated by high performance liquid chromatography of extracts prepared from both cell pellets and culture supernatants. We found <em>G. xylinus</em> synthesized GA, ABA and Z but did not produce IAA.

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