Towards Sustainable High‐Performance Thermoplastics: Synthesis, Characterization, and Enzymatic Hydrolysis of Bisguaiacol‐Based Polyesters

Pretreatment of biomass is a key step in the production of valuable products, including high-tech bacterial cellulose. The efficiency of five different pretreatment methods of Miscanthus and oat hulls for enzymatic hydrolysis and subsequent synthesis of bacterial cellulose (BC) was evaluated herein: Hydrothermobaric treatment, single-stage treatments with dilute HNO3 or dilute NaOH solution, and two-stage combined treatment with dilute HNO3 and NaOH solutions in direct and reverse order. The performance of enzymatic hydrolysis of pretreatment products was found to increase by a factor of 4−7. All the resultant hydrolyzates were composed chiefly of glucose, as the xylose percentage in total reducing sugars (RS) was 1−9%. The test synthesis of BC demonstrated good quality of nutrient media prepared from all the enzymatic hydrolyzates, except the hydrothermobaric treatment hydrolyzate. For biosynthesis of BC, single-stage pretreatments with either dilute HNO3 or dilute NaOH are advised due their simplicity and the high performance of enzymatic hydrolysis of pretreatment products (RS yield 79.7−83.4%).

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Study of Pretreatment and Enzymatic Hydrolysis on Microcrystalline Cellulose and HVS A4 Paper in Pressurized CO2 Media

Modern Applied Science ◽

10.5539/mas.v9n7p16 ◽

2015 ◽

Vol 9 (7) ◽

pp. 16 ◽

Cited By ~ 1

Author(s):

Ian Eka Pramudita ◽

Marcella Lauditta Noviana ◽

Henky Muljana

Keyword(s):

High Performance Liquid Chromatography ◽

Enzymatic Hydrolysis ◽

Microcrystalline Cellulose ◽

Levulinic Acid ◽

High Performance ◽

Model Compound ◽

Bioethanol Production ◽

Fixed Temperature ◽

Pre Treatment ◽

Hydrolysis Of

The aim of this work is to investigate the potential used of pressurized CO2 in the pre-treatment and the enzymatic hydrolysis of waste HVS A4 paperto produce a monomeric sugars (C5 and C6 sugars) which is a precursor for bioethanol production. Prior to the utilization of waste HVS A4 paper, the microcrystalline cellulose and HVS A4 paper were first used in the experiments as model compound in order to gain better insights of the process. The experiment consists of two main parts which are the preliminary experimentsto determine the best pretreatment conditionsbetween two selected pressure values (80 and 100 bar) at a fixed temperature (T = 75 oC) and the enzymatic hydrolysis experiments. In the latter, the microcrystalline and HVS A4 paper were hydrolyzed at different pressures (100 bar, 125 bar, and 150 bar) and at different cellulase intakes (1% (v/v), 3% (v/v), and 5% (v/v)) with a fixed temperature (50°C). The hydrolyzed products were analyzed with a High Performance Liquid Chromatography (HPLC) to quantify the monomeric sugars and to determine the presence of the side products (furfural, HMF and levulinic acid). Within the experimental range, a maximum glucose concentration of 7602.35 ppm and 4560.79 ppm are obtained for microcrystalline and HVS A4 paper, respectively. In addition, there are no furfural, HMF and levulinic acid detected in the products This study shows a potential used of pressurized CO2 in the pretreatment and enzymatic hydrolysis of the model compound and gives a better insight for further application.

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The influence of urea and formaldehyde on enzymatic hydrolysis of cellulose

Annals of WULS, Forestry and Wood Technology ◽

10.5604/01.3001.0014.4414 ◽

2020 ◽

Vol 110 ◽

pp. 92-96

Author(s):

Monika Marchwicka ◽

Anna Lesiak ◽

Andrzej Radomski

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Enzymatic Hydrolysis ◽

High Performance ◽

Glucose Yield ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Hydrolysis Of

Effect of selected urea and formaldehyde concentrations on glucose yield of enzymatic hydrolysis of cellulose was investigated. Urea and formaldehyde were added separately at the concentrations of 0.001, 0.002 and 0.005 g/cm3. Glucose was determined by high-performance liquid chromatography (HPLC). It was found that the used concentrations of urea didn’t influence glucose yield. In the case of formaldehyde, the results vary between used concentrations. The glucose yield of enzymatic hydrolysis of cellulose with the highest investigated concentration of formaldehyde (0.005 g/cm3) decreased by 50 %.

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Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover

Annals of WULS, Forestry and Wood Technology ◽

10.5604/01.3001.0015.3633 ◽

2021 ◽

Vol 115 ◽

pp. 29-36

Author(s):

Florentyna Akus-Szylberg ◽

Andrzej Antczak ◽

Janusz Zawadzki

Keyword(s):

Chemical Composition ◽

Enzymatic Hydrolysis ◽

Corn Stover ◽

High Performance ◽

Aqueous Ammonia ◽

Ammonia Solution ◽

Raw Material ◽

Different Temperatures ◽

Ammonia Pretreatment ◽

Hydrolysis Of

Effects of soaking aqueous ammonia pretreatment on chemical composition and enzymatic hydrolysis of corn stover. The aim of this research was to investigate the effect of applying two different temperatures of the soaking aqueous ammonia treatment on the chemical composition and enzymatic hydrolysis yield of the corn stover. Native corn stover as well as solid fractions after 20 h of alkali pretreatment performed at 15% ammonia solution and at 50 °C or 90 °C were analysed in terms of cellulose, holocellulose, lignin and extractives content. Both untreated and treated samples were subjected to the enzymatic hydrolysis and hydrolysates were examined with a high performance liquid chromatography (HPLC). Results indicated a significant development of enzymatic digestibility of the SAA treated biomass. Furthermore, a 38.7% and a 68.9% delignification levels in the biomass treated with ammonia at respectively 50 °C and 90 °C process comparing to the raw material were achieved.

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Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 50 °C

Annals of WULS, Forestry and Wood Technology ◽

10.5604/01.3001.0015.2373 ◽

2021 ◽

Vol 114 ◽

pp. 53-58

Author(s):

Monika Marchwicka

Keyword(s):

Enzymatic Hydrolysis ◽

High Performance ◽

Populus Trichocarpa ◽

Dry Mass ◽

Glucose Content ◽

Glucose Yield ◽

Enzymatic Hydrolysis Of Cellulose ◽

Hydrolysis Of Cellulose ◽

Influence Of Ph ◽

Hydrolysis Of

Influence of pH and Cellic® CTec2 enzymes dose on the glucose yield after enzymatic hydrolysis of cellulose at 50 °C. Cellulose obtained by the Kürschner-Hoffer method from the wood of 3-year-old poplar (Populus trichocarpa) was subjected to enzymatic hydrolysis. Cellic® CTec2 enzymes (Novozymes, Denmark) were used. The enzymatic hydrolysis was tested within the conditions recommended by the manufacturer and the literature. The process was carried out at 50 °C at various pH – 4.8, 5.0, 5.5 and enzymes doses - 25, 50 and 100 mg per 100 mg of the dry mass of cellulose. The process was ended after 24 h. The hydrolysates were analysed by high-performance liquid chromatography (HPLC) to determine the glucose content, and then the highest glucose yield. The highest glucose yield was obtained for pH 4.8 and 100 mg of enzymes per 100 mg of the dry mass of cellulose – 72 %.

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