MAIN TECHNOLOGICAL STAGES OF BACTERIAL CELLULOSE SYNTHESIS FROM EASILY RENEWABLE CELLULOSIC FEEDSTOCKS BY МEDUSOMYCES GISEVII SA-12 SYMBIOTIC CULTURE

2020 ◽  
pp. 290-292
Author(s):  
V. Budaeva ◽  
Е. Gladysheva ◽  
N. Shavyrkina ◽  
I. Pavlov ◽  
D. Golubev ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Synthesis ◽  
High Quality ◽  
Bacterial Nanocellulose ◽  
Oat Hulls ◽  
Pretreatment Method

A technology has been developed for the synthesis of bacterial nanocellulose from oat hulls and Miscanthus. The Мedusomyces gisevii Sa-12 microbial producer was found to synthesize bacterial nanocellulose of high quality, regardless of the feedstock type and pretreatment method thereof.

Bacterial Cellulose Synthesis in Kombucha by Gluconacetobacter sp and Saccharomyces sp

2012 ◽  
Vol 554-556 ◽  
pp. 1000-1003 ◽  
Author(s):  
Li Li Tan ◽  
Liang Ren ◽  
Yuan Yuan Cao ◽  
Xiao Lin Chen ◽  
Xin Yun Tang
Keyword(s):  
Bacterial Cellulose ◽  
Polynomial Regression ◽  
High Yield ◽  
Cellulose Synthesis ◽  
Affecting Factors ◽  
High Quality ◽  
Factors Affecting ◽  
Initial Ph ◽  
Pure Cultures ◽  
Major Factors

Strain Gluconacetobacter hansenii CGMCC1671 and Saccharomyces cerevisiae CGMCC1670 were applied to make traditional Kombucha with pure cultures to search for the optimum parameters of major factors affecting the yields and productivities of Bacterial cellulose (BC) in the beverage. Three culture factors were examined. The yields and productivities of BC and sugar consumed were measured after cultured statically for 22 days. After single factor test factors affecting the yields and productivities of BC have been optimized by response surface methodology (RSM). The quadratic polynomial regression equation reflecting BC yield and affecting factors was build up with Box-Behnken design principle. The optimal values of 10.37% inoculum, initial pH 4.96 and medium volume 77.13 mL in 250 mL flask were obtained with theoretical BC yield 300.093mg/g. BC yield of 279.579 mg/g was obtained with 6.84% deviation by validation test with the optimal parameters. The co-culture of pure strains of traditional Kombucha technique can be used to provide both high quality and high yield of BC in addition to producing high quality Kombucha beverage.

scholarly journals Pretreatments of Non-Woody Cellulosic Feedstocks for Bacterial Cellulose Synthesis

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1645 ◽  
Author(s):  
Ekaterina I. Kashcheyeva ◽  
Yulia A. Gismatulina ◽  
Vera V. Budaeva
Keyword(s):  
Enzymatic Hydrolysis ◽  
Bacterial Cellulose ◽  
High Performance ◽  
Combined Treatment ◽  
Single Stage ◽  
High Tech ◽  
Cellulose Synthesis ◽  
Oat Hulls ◽  
Naoh Solution ◽  
Hydrolysis Of

Pretreatment of biomass is a key step in the production of valuable products, including high-tech bacterial cellulose. The efficiency of five different pretreatment methods of Miscanthus and oat hulls for enzymatic hydrolysis and subsequent synthesis of bacterial cellulose (BC) was evaluated herein: Hydrothermobaric treatment, single-stage treatments with dilute HNO3 or dilute NaOH solution, and two-stage combined treatment with dilute HNO3 and NaOH solutions in direct and reverse order. The performance of enzymatic hydrolysis of pretreatment products was found to increase by a factor of 4−7. All the resultant hydrolyzates were composed chiefly of glucose, as the xylose percentage in total reducing sugars (RS) was 1−9%. The test synthesis of BC demonstrated good quality of nutrient media prepared from all the enzymatic hydrolyzates, except the hydrothermobaric treatment hydrolyzate. For biosynthesis of BC, single-stage pretreatments with either dilute HNO3 or dilute NaOH are advised due their simplicity and the high performance of enzymatic hydrolysis of pretreatment products (RS yield 79.7−83.4%).

scholarly journals Effect of Gluconacetobacter xylinus cultivation conditions on the selected properties of bacterial cellulose

2016 ◽  
Vol 18 (4) ◽  
pp. 117-123 ◽  
Author(s):  
Karol Fijałkowski ◽  
Anna Żywicka ◽  
Radosław Drozd ◽  
Marian Kordas ◽  
Rafał Rakoczy
Keyword(s):  
Bacterial Cellulose ◽  
Bacterial Strain ◽  
Point Of View ◽  
Synthesis Process ◽  
Cellulose Synthesis ◽  
Gluconacetobacter Xylinus ◽  
Water Release ◽  
Good Efficiency ◽  
Cellulose Fibrils ◽  
Culturing Conditions

Abstract The aim of the study was to analyze the changes in the parameters of bacterial cultures and bacterial cellulose (BC) synthesized by four reference strains of Gluconacetobacter xylinus during 31-day cultivation in stationary conditions. The study showed that the most visible changes in the analyzed parameters of BC, regardless of the bacterial strain used for their synthesis, were observed in the first 10–14 days of the experiment. It was also revealed, that among parameters showing dependence associated with the particular bacterial strain were the rate and period of BC synthesis, the growth rate of bacteria anchored to the cellulose fibrils, the capacity to absorb water and the water release rate. The results presented in this work may be useful in the selection of optimum culturing conditions and period from the point of view of good efficiency of the cellulose synthesis process.

scholarly journals Physicochemical characterization of high-quality bacterial cellulose produced by Komagataeibacter sp. strain W1 and identification of the associated genes in bacterial cellulose production

RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 45145-45155 ◽  
Author(s):  
Shan-Shan Wang ◽  
Yong-He Han ◽  
Yu-Xuan Ye ◽  
Xiao-Xia Shi ◽  
Ping Xiang ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Genome Sequencing ◽  
Physicochemical Characterization ◽  
High Quality ◽  
Cellulose Production ◽  
Bacterial Cellulose Production

Komagataeibacter sp. W1 produced high-quality BC, the properties and synthesis mechanisms of which were analyzed by SEM, XRD and FTIR, and genome sequencing, respectively.

Metal-ion-imprinted thermo-responsive materials obtained from bacterial cellulose: synthesis, characterization, and adsorption evaluation

2019 ◽  
Vol 7 (19) ◽  
pp. 11742-11755 ◽  
Author(s):  
Zhiming Li ◽  
Hua Tian ◽  
Yanyan Yuan ◽  
Xueqiong Yin ◽  
Xin Wei ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Metal Ion ◽  
Analytical Techniques ◽  
Cellulose Synthesis ◽  
Structure And Properties ◽  
Responsive Materials ◽  
Ion Imprinted

A novel gradient heating procedure was developed to fabricate a targeted metal-ion-imprinted thermo-responsive material from bacterial cellulose using Cu2+ as a template and N-isopropylacrylamide (NIPAM) as a monomer; moreover, its structure and properties were analysed via various analytical techniques.

Bioengineering tunable porosity in bacterial nanocellulose matrices

Soft Matter ◽  
2019 ◽  
Vol 15 (45) ◽  
pp. 9359-9367 ◽  
Author(s):  
Zahra Ashrafi ◽  
Lucian Lucia ◽  
Wendy Krause
Keyword(s):  
Physical Properties ◽  
Bacterial Cellulose ◽  
Culture Conditions ◽  
Bacterial Nanocellulose ◽  
Nutrient Sources ◽  
Specific Culture

Our work provides the first accounting of how specific culture conditions, i.e., carbon nutrient sources, control morphological and physical properties in bacterial cellulose filaments.

scholarly journals Bacterial cellulose synthesis mechanism of facultative anaerobe Enterobacter sp. FY-07

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kaihua Ji ◽  
Wei Wang ◽  
Bing Zeng ◽  
Sibin Chen ◽  
Qianqian Zhao ◽  
...  
Keyword(s):  
Bacterial Cellulose ◽  
Cellulose Synthesis ◽  
Facultative Anaerobe ◽  
Synthesis Mechanism

Bacterial Nanocellulose based wound dressings: Current and Future Prospects

2021 ◽  
Vol 27 ◽  
Author(s):  
Hitesh Chopra ◽  
Shweta Gandhi ◽  
Rupesh Kumar Gautam ◽  
Mohammad Amjad Kamal
Keyword(s):  
Wound Healing ◽  
Bacterial Cellulose ◽  
Preparation Method ◽  
Treatment Options ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Future Prospects ◽  
Bacterial Nanocellulose

: Although there is a big heap of treatment options available for the wound and burns dressings, improvements in technology are still required. Bacterial cellulose is a polymer derived from the microbiological world and has shown some promising properties that recommend it as a wound healing therapeutic. Moreover, bacterial cellulose can be nanosized to form Bacterial Nanocellulose (BNC), enhancing its properties. Most importantly, in addition to its inherent antibacterial properties, BNC can be used to deliver drugs. This article presents a birds-eye view of the preparation method and applications of BNC based wound dressings.

The Effect of Growth, Migration and Bacterial Cellulose Synthesis of Gluconacetobacter xylinus in Presence of Direct Current Electric Field Condition

2012 ◽  
Vol 550-553 ◽  
pp. 1108-1113 ◽  
Author(s):  
Lin Yan ◽  
Shi Ru Jia ◽  
Xin Tong Zheng ◽  
Cheng Zhong ◽  
Miao Liu ◽  
...  
Keyword(s):  
Electric Field ◽  
Bacterial Cellulose ◽  
Electric Fields ◽  
Direct Current ◽  
Cellulose Fiber ◽  
Sem Analysis ◽  
Cellulose Synthesis ◽  
Gluconacetobacter Xylinus ◽  
Growth State ◽  
Dc Electric Field

In this study, the movement and orientation of bacteria cells were controlled by direct current(DC) electric fields, result in altering alignment of bacterial cellulose nanofiber and further changing the 3-dimensional network structure of bacterial cellulose. A modified swarm plate assay was performed to investigate the migration of Gluconacetobacter xylinus cells which exposed in DC electric field. It suggested that the cells moved toward to negative pole and with the increasement of the electric field strength the velocity will also increase. The SEM analysis demonstrated that the cellulose fiber bundles which synthesized at 1V/cm have lager diameter and a trend toward one direction. Meanwhile the growth state of G.xylinus in the presence of DC electric field was also being observed.

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