scholarly journals Delayed Addition of Template Molecules Enhances the Binding Properties of Diclofenac-Imprinted Polymers

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1178 ◽  
Author(s):  
Laura Anfossi ◽  
Simone Cavalera ◽  
Fabio Di Nardo ◽  
Giulia Spano ◽  
Cristina Giovannoli ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Polymerization Process ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Binding Selectivity ◽  
Binding Properties ◽  
Imprinted Polymers ◽  
Binding Data ◽  
Binding Isotherms ◽  
The Stability

It has been reported that in the molecular imprinting technique, the use of preformed oligomers instead of functional monomers increases the stability of the non-covalent interactions with the template molecule, providing a sharp gain in terms of binding properties for the resulting imprinted polymer. Based on this theory, we assumed that the delayed addition of template molecules to a polymerization mixture enhances the binding properties of the resulting polymer. To verify this hypothesis, we imprinted several mixtures of 4-vinylpyridine/ethylene dimethacrylate (1:6 mol/mol) in acetonitrile by adding diclofenac progressively later from the beginning of the polymerization process. After polymerization, the binding isotherms of imprinted and non-imprinted materials were measured in acetonitrile by partition equilibrium experiments. Binding data confirm our hypothesis, as imprinted polymers prepared by delayed addition, with delay times of 5 and 10 min, showed higher binding affinity (Keq = 1.37 × 104 L mol−1 and 1.80 × 104 L mol−1) than the polymer obtained in the presence of template at the beginning (Keq = 5.30 × 103 L mol−1). Similarly, an increase in the imprinting factor measured vs. the non-imprinted polymer in the binding selectivity with respect to mefenamic acid was observed. We believe that the delayed addition approach could be useful in prepar imprinted polymers with higher binding affinity and increased binding selectivity in cases of difficult imprinting polymerization.

Preparation of Henriol C Molecularly Imprinted Polymer Microspheres

2014 ◽  
Vol 664 ◽  
pp. 34-37
Author(s):  
Yong Ming Luo ◽  
Yong Dong Luo ◽  
Fang You Chen ◽  
Xue Lian Zhang ◽  
Xiao Ying Yin
Keyword(s):  
Binding Affinity ◽  
Solid Phase ◽  
Scatchard Analysis ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Functional Monomers ◽  
Novel Method ◽  
Stereo Selectivity ◽  
High Binding Affinity

Molecular imprinted polymer micrcosheres (MIPMs) were prepared through precipitation polymerization by henriol C using as the template molecule. The morphologies of synthesized MIPMs were characterized by scanning electronmicroscope (SEM). Systematic investigations of the influences of key synthetic conditions, including functional monomers, cross-linkers and porogens, on the morphologies, yields and the recognition properties of the MIPs were conducted. The results indicated that the morphologies of MIPs with DVB as cross-linker was perfect, but their binding affinity is lower than that of MIPs with TRIM or EDMA as cross-linkers. And particle size of MIPs with TRIM as cross-linkers is small but with high binding affinity. Scatchard analysis revealed that the homogeneous binding sites were formed in the polymers. The application of MIPs with high affinity and excellent stereo-selectivity toward henriol C in solid-phase extraction (SPE) column might offer a novel method for the enrichment and determination of sesquiterpenoids in the traditional herbal medicine.

Characterization and Evaluation of Binding Properties of Salvianolic Acid a Imprinted Polymers Prepared by Precipitation Polymerization

2011 ◽  
Vol 239-242 ◽  
pp. 2423-2426
Author(s):  
Lin Min Zhu ◽  
Shao Ping Fu ◽  
Lin Ying Li ◽  
Jing Bo Zhu
Keyword(s):  
Dissociation Constants ◽  
Precipitation Polymerization ◽  
Scatchard Analysis ◽  
Template Molecule ◽  
Binding Properties ◽  
Imprinted Polymers ◽  
Salvianolic Acid ◽  
Salvianolic Acid A ◽  
Equilibrium Dissociation Constants ◽  
Equilibrium Dissociation

Molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization using salvianolic acid A (Sal A), acrylamide (AA), ethylene glycol dimethacrylate (EGDMA) and acetonitrile as template molecule, functional monomer, cross-linker and solvent, respectively. The morphology of the obtained polymers was characterized by scanning electron microscopy (SEM). The effect of different polymerization conditions (solvent volume, solvent and template amount) on the size and shape of particles was investigated. The binding properties of the imprinted polymers were evaluated through the equilibrium rebinding experiments. Scatchard analysis revealed that two classes of binding sites were formed in the imprinted polymers with equilibrium dissociation constants of 0.33 μmol·mL-1and 0.07 μmol·mL-1, respectively. Besides Sal A, two structurally related compounds, protocatechuic aldehyde (Pra) and salvianolic acid B (Sal B), were employed for molecular selectivity tests. The results indicated that the imprinted polymers exhibited good selectivity and specificity toward Sal A.

scholarly journals Synthesis of Molecularly Imprinted Polymerand its Molecular Recognition PropertiesofN-Acetylneuraminic Acid

2007 ◽  
Vol 4 (4) ◽  
pp. 611-619 ◽  
Author(s):  
Hsin-Hung Pan ◽  
Wen-Chien Lee ◽  
Chin-Yin Hung ◽  
Ching-Chiang Hwang
Keyword(s):  
Specific Binding ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Acetylneuraminic Acid ◽  
Binding Isotherms ◽  
Molecular Imprinting Technique ◽  
Guest System ◽  
Cross Linker ◽  
Principal Advantage

A molecular imprinting technique was applied in this work to detectN-acetylneuraminic acid (Neu5Ac) and its analogue structure. Twomolecularly imprinted polymers (MIP) were prepared using Neu 5Ac as the template molecule, as well as methacrylic acid (MAA) or 4-vinylpyridine (4-Vpy) and ethyleneglycol dimethacrylate (EGDMA) as the functionalmonomer and cross-linker, respectively. Free radical polymerization was carried out at 4°C under UV radiation or thermal (60°C) polymerization. MIP thus obtained were ground into 11∼25µm and 25-44µm. The binding results from Neu5Ac solution, mannose (Man) solution andN-acetyl-Dmannosamine (ManNAc) solution performed by Neu5Ac-MIP showed specific binding toward Neu5Ac rather than other analogue compounds on the host-guest system. The values of capacity for Neu5Ac-MIP were measured and experimental results were further used for simulation to obtain the binding isotherms. The principal advantage of this method is thatNeu5Ac- MIP can recognize Neu5Ac and its analogue compounds.

scholarly journals Sensing by Molecularly Imprinted Polymer: Evaluation of the Binding Properties with Different Techniques

Sensors ◽  
2019 ◽  
Vol 19 (6) ◽  
pp. 1344 ◽  
Author(s):  
Maria Pesavento ◽  
Simone Marchetti ◽  
Letizia De Maria ◽  
Luigi Zeni ◽  
Nunzio Cennamo
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Association Constant ◽  
Adsorption Model ◽  
Imprinted Polymer ◽  
Binding Properties ◽  
Molecularly Imprinted ◽  
Interaction Sites ◽  
Batch Equilibration ◽  
Binding Isotherms

The possibility of investigating the binding properties of the same molecularly imprinted polymer (MIP), most probably heterogeneous, at various concentration levels by different methods such as batch equilibration and sensing, is examined, considering two kinds of sensors, based respectively on electrochemical and surface plasmon resonance (SPR) transduction. As a proof of principle, the considered MIP was obtained by non-covalent molecular imprinting of 2-furaldehyde (2-FAL). It has been found that different concentration ranges of 2-FAL in aqueous matrices can be measured by the two sensing methods. The SPR sensor responds in a concentration range from 1 × 10−4 M down to about 1 × 10−7 M, while the electrochemical sensor from about 5 × 10−6 M up to about 9 × 10−3 M. The binding isotherms have been fit to the Langmuir adsorption model, in order to evaluate the association constant. Three kinds of sites with different affinity for 2-FAL have been detected. The sites at low affinity are similar to the interaction sites of the corresponding NIP since they have a similar association constant. This is near to the affinity evaluated by batch equilibration too. The same association constant has been evaluated in the same concentration range. The sensing methods have been demonstrated to be very convenient for the characterization of the binding properties of MIP in comparison to the batch equilibration, in terms of reproducibility and low amount of material required for the investigation.

scholarly journals Synergetic Effect of Dual Functional Monomers in Molecularly Imprinted Polymer Preparation for Selective Solid Phase Extraction of Ciprofloxacin

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2788
Author(s):  
Ut Dong Thach ◽  
Hong Hanh Nguyen Thi ◽  
Tuan Dung Pham ◽  
Hong Dao Mai ◽  
Tran-Thi Nhu-Trang
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
Solid Phase ◽  
Template Molecule ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers ◽  
Dual Functional

Background: Ciprofloxacin (CIP), an important broad-spectrum fluoroquinolone antibiotic, was often used as a template molecule for the preparation of imprinted materials. In this study, methacrylic acid and 2-vinylpyridine were employed for the first time as dual functional monomers for synthesizing ciprofloxacin imprinted polymers. Methods: The chemical and physicochemical properties of synthesized polymers were characterized using Fourier transform-infrared spectroscopy, thermogravimetric analysis-differential scanning calorimetry, scanning electron microscopy, and nitrogen adsorption-desorption isotherm. The adsorption properties of ciprofloxacin onto synthesized polymers were determined by batch experiments. The extraction performances were studied using the solid phase extraction and HPCL-UV method. Results: The molecularly imprinted polymer synthesized with dual functional monomers showed a higher adsorption capacity and selectivity toward the template molecule. The adsorbed amounts of ciprofloxacin onto the imprinted and non-imprinted polymer were 2.40 and 1.45 mg g−1, respectively. Furthermore, the imprinted polymers were employed as a selective adsorbent for the solid phase extraction of ciprofloxacin in aqueous solutions with the recovery of 105% and relative standard deviation of 7.9%. This work provides an alternative approach for designing a new adsorbent with high adsorption capacity and good extraction performance for highly polar template molecules.

scholarly journals An Optimization of Functional Monomer, A Preliminary Study of Meropenem Imprinted Polymer as Selective Sorbent

2018 ◽  
Vol 3 (2) ◽  
pp. 103 ◽  
Author(s):  
Lasmaryna Sirumapea ◽  
Muhammad Ali Zulfikar ◽  
M. Bachri Amran ◽  
Anita Alni
Keyword(s):  
High Selectivity ◽  
Polymerization Process ◽  
Template Molecule ◽  
Functional Monomer ◽  
Imprinted Polymer ◽  
Lactam Ring ◽  
Preliminary Study ◽  
Main Components ◽  
The Right ◽  
Selection Of

<p>Molecularly imprinted polymer is a polymer that synthesized together with targeted molecule (analyte) through polymerization process. When the polymerization has completed, extraction is taken to pull the analyte out of the polymer, and it will give a specific “frame” of targeted molecule which leave cavities and further is used to give specific recognition of the analyte. Functional monomer, template molecule, crosslinker agent, porogenic solvent and initiator are the main components involved in the synthesis of this kind of polymer. The right functional monomer is needed to make polymer building block with high selectivity when used as sorbent. The selection of suitable and precise functional monomer has been elaborated in this study. methacrylate acid, itaconic acid and acrylamide is then studies as functional monomer that will be chosen to give the best polymer performance as sorbent. From IR spectrum results, it can be concluded that polymer has been synthesized successfully. Peak at 1157 cm<sup>-1</sup> which is a peak of C-N bond from β-lactam ring support this statement.  Finally, it can be concluded that methacrylate acid is the best functional monomer for making polymer as sorbent for meropenem</p>

scholarly journals The Use of Computational Methods for the Development of Molecularly Imprinted Polymers

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 2841
Author(s):  
Ian A. Nicholls ◽  
Kerstin Golker ◽  
Gustaf D. Olsson ◽  
Subramanian Suriyanarayanan ◽  
Jesper G. Wiklander
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Polymerization Process ◽  
Current Status ◽  
Computational Techniques ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Computational Approaches ◽  
Imprinted Polymers ◽  
Polymerization Mixture

Recent years have witnessed a dramatic increase in the use of theoretical and computational approaches in the study and development of molecular imprinting systems. These tools are being used to either improve understanding of the mechanisms underlying the function of molecular imprinting systems or for the design of new systems. Here, we present an overview of the literature describing the application of theoretical and computational techniques to the different stages of the molecular imprinting process (pre-polymerization mixture, polymerization process and ligand–molecularly imprinted polymer rebinding), along with an analysis of trends within and the current status of this aspect of the molecular imprinting field.

Molecularly Imprinted Polymer for Preparation and Adsorption of Phenylalanine

2011 ◽  
Vol 306-307 ◽  
pp. 638-641 ◽  
Author(s):  
Fu Guang Lu ◽  
Yu Lei Xi ◽  
Zhen Lv ◽  
Lu Lu Fan ◽  
Hua Min Qiu ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Template Molecule ◽  
Binding Properties ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Molecular Imprinting Technology ◽  
Particle Size And Morphology ◽  
Size And Morphology ◽  
Polymerization Conditions

Molecular imprinting technology was employed to produce one kind of Phenylalanine ( Phe) molecularly imprinted polymers (MIP)by precipitation polymerization using Phe, anhydrous alcohol, acrylamide, ethylene glycol dimethacrylate, and azobisisobutyronitrile as template, porogen, functional monomer, cross-linker and initiator respectively. In this study, the polymerization conditions were optimized. The template on particle size and morphology of polymers were investigated in detail by scanning electron microscopy (SEM) and adsorption isotherm determination. The binding properties of Phe on imprinted polymers were evaluated in water by equilibrium rebinding experiments, and the maximum number of adsorption was 0.60 mmol/g. It is indicated that the existence of binding sites in imprinted polymers was proved and the binding sites showed good specific and selective capability to the template molecule Phe. So the polymers would be used to separate Phe from medicine and food.

Sythesis of Molecularly Imprinted Polymers for the Binding and Recognition of Nonylphenol

2013 ◽  
Vol 641-642 ◽  
pp. 55-59 ◽  
Author(s):  
Yan Jie Bi ◽  
Wen Yan Li ◽  
Chen Yuan ◽  
Bin Song Wang
Keyword(s):  
Molecularly Imprinted Polymers ◽  
High Selectivity ◽  
Sol Gel ◽  
Template Molecule ◽  
Binding Properties ◽  
Adsorption Selectivity ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Selective Enrichment ◽  
Sol Gel Process

In this paper, molecularly imprinted polymers with high selectivity for nonylphenol (NP) were synthesized by sol-gel process using 4-vinylpyridine (4-Vpy) as functional monomer, ethyleneglycol dimethacrylate as crosslinker (EGDMA), azobisisobutyronitrile (AIBN) as initiator, NP as template molecules. The performance of imprinted material was evaluated by adsorption kinetic, adsorption isotherm and adsorption selectivity. The results indicated that this material had not only binding properties but also high selectivity to the template molecule, which had good application prospects in the selective enrichment and separation of NP for pretreatment and analysis of complex environmental samples.

scholarly journals PEMILIHAN MONOMER FUNGSIONAL PADA MOLECULARLY IMPRINTED POLYMER (MIP) DIAZEPAM DENGAN TEKNIK KOMPUTASI

2019 ◽  
Vol 9 (2) ◽  
pp. 1
Author(s):  
Dang Soni ◽  
Aliya Nur Hasanah ◽  
Mutakin Mutakin
Keyword(s):  
Binding Affinity ◽  
Molecularly Imprinted Polymer ◽  
Molecularly Imprinted Polymers ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Imprinted Polymers

Teknik preparasi yang tepat dapat mempengaruhi hasil analisis sehingga pemilihan preparasi sampel yang tepat harus diperhitungkan terutama untuk analisis diazepam dalam matriks sampel yang rumit seperti serum dan urin. Molecularly imprinted polymers (MIP) merupakan salah satu teknik preparasi yang efektif untuk mengekstrak analit target dari matriks kompleks sebelum analisis. Pemilihan monomer fungsional merupakan hal terpenting dalam pembuatan MIP yang baik, sehingga pemilihannya sesuai dengan sifat fisikokimia monomer dan diazepam sebagai tamplate. Interaksi monomer dan tamplate yang memberikan nilai binding affinity yang paling baik pada monomer asam adalah p-vinylbenzoic acid sebesar -3,3 kkal/mol, pada monomer basa adalah 5-[1-(2,3-dimethylphenyl)ethenyl]-1H-imidazole sebesar -3,8 kkal/mol dan pada monomer netral adalah ethylstyrene sebesar -3,6 kkal/mol. Dari semua monomer yang diuji baik monomer asam,basa atau netral yang menghasilkan binding affinity yang baik adalah monomer basa yaitu 5-[1-(2,3-dimethylphenyl)ethenyl]-1H-imidazole sebesar -3,8 kkal/mol yang artinya template diazepam akan berinteraksi dengan baik dengan monomer tersebut. Kata kunci: Diazepam, Molekular Imprinting Polimer (MIP), Pemilihan Monomer Fungsional

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