Synthesis of Molecularly Imprinted Polymerand its Molecular Recognition PropertiesofN-Acetylneuraminic Acid

A molecular imprinting technique was applied in this work to detectN-acetylneuraminic acid (Neu5Ac) and its analogue structure. Twomolecularly imprinted polymers (MIP) were prepared using Neu 5Ac as the template molecule, as well as methacrylic acid (MAA) or 4-vinylpyridine (4-Vpy) and ethyleneglycol dimethacrylate (EGDMA) as the functionalmonomer and cross-linker, respectively. Free radical polymerization was carried out at 4°C under UV radiation or thermal (60°C) polymerization. MIP thus obtained were ground into 11∼25µm and 25-44µm. The binding results from Neu5Ac solution, mannose (Man) solution andN-acetyl-Dmannosamine (ManNAc) solution performed by Neu5Ac-MIP showed specific binding toward Neu5Ac rather than other analogue compounds on the host-guest system. The values of capacity for Neu5Ac-MIP were measured and experimental results were further used for simulation to obtain the binding isotherms. The principal advantage of this method is thatNeu5Ac- MIP can recognize Neu5Ac and its analogue compounds.

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Application of Response Surface Methodology to Synthesize Appropriate Molecularly Imprinted Polymer for Diazinon

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.605.67 ◽

2014 ◽

Vol 605 ◽

pp. 67-70 ◽

Cited By ~ 1

Author(s):

Mohsen Rahiminezhad ◽

Seyed Jamaleddin Shahtaheri ◽

Mohammad Reza Ganjali ◽

Abbas Rahimi Rahimi Forushani

Keyword(s):

Molecular Imprinting ◽

Molecularly Imprinted Polymer ◽

Binding Sites ◽

Capillary Electrochromatography ◽

Template Molecule ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Functional Monomers ◽

Molecular Imprinting Technology ◽

Cross Linker

Molecular imprinting technology has become an interesting research area to the preparation of specific sorbent material for environmental and occupational sample preparation techniques (1). In the molecular imprinting technology, specific binding sites have been formed in polymeric matrix, which often have an affinity and selectivity similar to antibody-antigen systems (2). In molecular imprinted technology, functional monomers are arranged in a complementary configuration around a template molecule, then, cross-linker and solvent are also added and the mixture is treated to give a porous material containing nono-sized binding sites. After extraction of the template molecule by washing, vacant imprinted sites will be left in polymer, which are available for rebinding of the template or its structural analogue (3). The stability, convention of preparation and low cost of these materials make them particularly attractive (4). These synthetic materials have been used for capillary electrochromatography (5), chromatography columns (6), sensors (7), and catalyze system (8). Depending on the molecular imprinting approach, different experimental variables such as the type and amounts of functional monomers, porogenic solvent, initiator, monomer to cross-linker ratio, temperature, and etc may alter the properties of the final polymeric materials. In this work, chemometric approach based on Central Composite Design (CCD) was used to design the experiments as well as to find the optimum conditions for preparing appropriate diazinon molecularly imprinted polymer.

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Fluorescence signaling molecularly imprinted polymers for antibiotics prepared via site-directed post-imprinting introduction of plural fluorescent reporters within the recognition cavity

Journal of Materials Chemistry B ◽

10.1039/c6tb02000c ◽

2016 ◽

Vol 4 (44) ◽

pp. 7138-7145 ◽

Cited By ~ 10

Author(s):

Hirobumi Sunayama ◽

Takeo Ohta ◽

Atsushi Kuwahara ◽

Toshifumi Takeuchi

Keyword(s):

Molecular Imprinting ◽

Molecularly Imprinted Polymers ◽

Fluorescent Dyes ◽

Specific Binding ◽

Fluorescence Signal ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Fluorescent Reporters ◽

Binding Event ◽

Fluorescence Signaling

An antibiotic-imprinted cavity with two different fluorescent dyes was prepared by molecular imprinting and subsequent post-imprinting modifications (PIMs), for the readout of a specific binding event as a fluorescence signal.

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Synthesis and characterization of molecularly imprinted polymers with modified rosin as a cross-linker and selective SPE-HPLC detection of basic orange II in foods

Analytical Methods ◽

10.1039/c4ay00810c ◽

2014 ◽

Vol 6 (16) ◽

pp. 6397-6406 ◽

Cited By ~ 16

Author(s):

Xiaoyan Li ◽

Mei Li ◽

Junjie Li ◽

Fuhou Lei ◽

Xiaomeng Su ◽

...

Keyword(s):

Molecularly Imprinted Polymers ◽

Solid Phase ◽

Selective Extraction ◽

Synthesis And Characterization ◽

Orange Ii ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Cross Linker

A novel sample clean-up technique, i.e., molecularly imprinted solid-phase extraction (MISPE) combined with HPLC, was developed and validated for the selective extraction and determination of basic orange II in foods.

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Improving molecularly imprinted nanogels by pH modulation

RSC Advances ◽

10.1039/c5ra16777a ◽

2015 ◽

Vol 5 (110) ◽

pp. 91018-91025 ◽

Cited By ~ 3

Author(s):

Zijie Zhang ◽

Juewen Liu

Keyword(s):

Specific Binding ◽

Template Molecule ◽

Molecularly Imprinted

Non-specific binding between template molecule and the imprinted gel is significantly reduced by pH modulation.

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Synthesis of a novel cross-linker doubles as a functional monomer for preparing a water compatible molecularly imprinted polymer

Analytical Methods ◽

10.1039/c4ay01651c ◽

2014 ◽

Vol 6 (23) ◽

pp. 9483-9489 ◽

Cited By ~ 3

Author(s):

Xiao Zhang ◽

Feng Shen ◽

Zhe Zhang ◽

Yue Xing ◽

Xueqin Ren

Keyword(s):

Molecularly Imprinted Polymer ◽

Naproxen Sodium ◽

Functional Monomer ◽

Imprinted Polymer ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Bifunctional Monomer ◽

Cross Linker

A new bifunctional monomer acting as both a cross-linker and a functional monomer was synthesized and applied in the preparation of water-compatible naproxen sodium imprinted polymers.

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Practice and Synthetize Molecularly Imprinted Polymers for Seperating Target Neuro-Protective Compounds from TCM with Less Pollution

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.955-959.239 ◽

2014 ◽

Vol 955-959 ◽

pp. 239-242

Author(s):

Ping Geng ◽

Qing Shan Liu ◽

Kebaituli Gulibanumu ◽

Xu Li ◽

Ke Qin Li ◽

...

Keyword(s):

Electron Microscope ◽

Molecularly Imprinted Polymers ◽

Extraction Method ◽

Template Molecule ◽

Ginsenoside Rb1 ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Synthetic Materials ◽

Scatchard Equation ◽

Environmentally Friendly Method

Molecularly imprinted polymers (MIPs) are synthetic materials that can be the environmental protection extraction method in TCM research and industry. They can overcome the defects of traditional extract methods and environmental pollution. In our research, MIPs were prepared by precipitation polymerization with neuro-protective picroside I and ginsenoside Rb1 as the template molecule. Moreover, the morphology of MIPs was characterized by electron microscope scanning and the static adsorption capacity was measured by the Scatchard equation. Finally, MIPs were made into MIP-SPE columns to enrich the template molecule and its analogues comparing with C18-SPE column and the results show that MIPs have good affinity and selectivity towards the Rb1 and Picroside I in SPE columns. This research may offer a more environmentally friendly method to extract active compounds in the traditional herbal.

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Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products

Scientific Reports ◽

10.1038/s41598-021-02625-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Ziqi Xie ◽

Yunjing Luo ◽

Zhen Na ◽

Wei Zhang ◽

Yufei Zong

Keyword(s):

Electron Microscopy ◽

Molecularly Imprinted Polymers ◽

Solid Phase ◽

Soy Sauce ◽

Template Molecule ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Magnetic Molecularly Imprinted Polymers ◽

Relative Standard ◽

Surface Molecular Imprinting Technique

AbstractIn this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated that the diameter of the Gen-MMIPs was approximately 500 nm. Via analysis with a vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs was determined to be 24.79 emu g−1. Fourier transform infrared (FT-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiments suggested that the genistein adsorption capability of Gen-MMIPs was 5.81 mg g−1, and adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction (dSPE) adsorbents combined with HPLC were used to selectively separate genistein in soy sauce samples, and the recoveries ranged from 85.7 to 88.5% with relative standard deviations (RSDs) less than 5%, which proved that this method can be used for the detection of genistein residues in real samples.

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Synthesis and Characterization of Genistein Magnetic Molecularly Imprinted Polymers and Its Application in Soy Sauce Products

10.21203/rs.3.rs-774651/v1 ◽

2021 ◽

Author(s):

Ziqi Xie ◽

Yunjing Luo ◽

Zhen Na ◽

Wei Zhang ◽

Yunfei Zong

Keyword(s):

Electron Microscopy ◽

Molecularly Imprinted Polymers ◽

Solid Phase ◽

Soy Sauce ◽

Template Molecule ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Magnetic Molecularly Imprinted Polymers ◽

Relative Standard ◽

Surface Molecular Imprinting Technique

Abstract In this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed by surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs were characterized by using of scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated the diameters of Gen-MMIPs were about 500 nm. Through the technique of vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs were detected as 24.79 emu/g. Fourier transform infrared (FR-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiment suggested the adsorption capability of Gen-MMIPs to genistein were 1.55 mg/g, and 2 the adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction adsorbent combined with HPLC was used to selectively separate genistein in soy sauce samples, the recoveries were ranged from 85.7% to 88.5% with the relative standard deviations (RSD) less than 5%, which proved this method can be used for the detection of genistein residues in real samples.

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SYNTHESIS OF MOLECULARLY IMPRINTED POLYMERS (MIPS) USED FOR ESTIMATION OF BETAMETHASONE DISODIUM PHOSPHATE (BMSP) USING DIFFERENT FUNCTIONAL MONOMERS

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i1.947 ◽

2020 ◽

Vol 51 (1) ◽

Author(s):

Al-Abbasi & et al.

Keyword(s):

Ethylene Glycol ◽

Molecularly Imprinted Polymers ◽

Sodium Phosphate ◽

Solid Phase ◽

Pharmaceutical Preparation ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Relative Standard ◽

Cross Linker ◽

Betamethasone Sodium Phosphate

Betamethasone sodium phosphate (BMSP) selective molecularly imprinted polymers(MIPs) were based on ion-pair by prepared four polymers(MIPs) using BMSP as the template a well as (Acryl amide) (AAM), 2-Acrylamido-2-Methyl-1-Propane sulphonic Acid (2-AAMMPSA as monomer, used N,N-ethylenebismethacrylamide (EBMAA) ,ethylene glycol dimethacrylate ethylene glycol(EGDMAC), N, N-methylene bisacrylamide (NNMBAAM)) as cross linker and used benzoyl peroxide as initiator . NIPs prepared by using the same composition of MIPs except the template (BMSP). The MIPs were prepared using variation ratio of monomer and cross linker .These MIPs applicate as solid phase extraction for determination BMSP in pharmaceutical preparation used UV as detector .the results gave good response, where the reconstruction percentage (Rec%) value of BMSP drug took the range (99.058149 % - 101.887004 %), and the relative standard deviation (RSD%) value took the range (0.224149 % - 0.743651 %) for standard solution and Rec% took values of (98.400035 - 99.404218) %, and RSD% took values of (0.572589 - 1.012777) % of BMSP drug for the Betamethasone sodium phosphate pharmaceutical.

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Synthesis of Magnetic Molecularly Imprinted Polymer Sorbents for Isolation of Parabens from Breast Milk

Materials ◽

10.3390/ma13194328 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4328

Author(s):

Martyna Pajewska-Szmyt ◽

Ewelina Biniewska ◽

Bogusław Buszewski ◽

Renata Gadzała-Kopciuch

Keyword(s):

Breast Milk ◽

Limit Of Detection ◽

Polymer Surface ◽

Magnetic Core ◽

Template Molecule ◽

Limit Of Quantification ◽

Rapid Separation ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Polymer Sorbents

Magnetic molecularly imprinted polymers (MMIPs) are an invaluable asset in the development of many methods in analytical chemistry, particularly sample preparation. Novel adsorbents based on MMIPs are characterized by high selectivity towards a specific analyte due to the presence of a specific cavity on their polymer surface, enabling the lock–key model interactions to occur. In addition, the magnetic core provides superparamagnetic properties that allow rapid separation of the sorbent from the sample solution. Such a combination of imprinted polymers with a magnetic core has an innovative influence on the development of separation techniques. Hence, the present study describes the synthesis of MMIPs with 17β-estradiol used as a template molecule in the production of imprinted polymers. The as-prepared sorbent was used for a sorption/desorption study of five parabens from breast milk samples. The obtained results were characterized by sorption efficiency exceeding 92%, which shows the high affinity of the analytes to the functional groups on the sorbent. The final determination of the selected analytes was done with high-performance liquid chromatography using a fluorometric detector. The determined linearity ranges for selected parabens were characterized by high determination coefficients (r2 from 0.9992 to 0.9999), and the calculated limit of detection (LOD) and limit of quantification (LOQ) for the identified compounds were low (LOD from 1.1–2.7 ng mL−1; LOQ from 3.6–8.1 ng mL−1), which makes their quantitative analysis in real samples feasible.

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