scholarly journals Color-Indicating, Label-Free, Dye-Doped Liquid Crystal Organic-Polymer-Based-Bioinspired Sensor for Biomolecule Immunodetection

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2294
Author(s):  
Haw-Ming Huang ◽  
Er-Yuan Chuang ◽  
Fu-Lun Chen ◽  
Jia-De Lin ◽  
Yu-Cheng Hsiao
Keyword(s):  
Liquid Crystal ◽  
Smart Phone ◽  
Organic Polymer ◽  
The Novel ◽  
Label Free ◽  
Home Test ◽  
Sensing Technology ◽  
Highly Sensitive ◽  
Vertically Aligned ◽  
Alignment Layers

The highly sensitive interfacial effects between liquid crystal (LC) and alignment layers make LC-bioinspired sensors an important technology. However, LC-bioinspired sensors are limited by quantification requiring a polarized microscope and expensive equipment, which makes it difficult to commercialize LC-bioinspired sensors. In this report, we first demonstrate that dye-doped LC (DDLC) chips coated with vertically aligned layers can be employed as a new LC-bioinspired sensing technology. The DDLC-bioinspired sensor was tested by detecting bovine serum albumin (BSA) and immunocomplexes of BSA pairs. The intensities of the dye color of the DDLC-bioinspired sensor can be changed with the concentrations of biomolecules and immunocomplexes. A detection limit of 0.5 µg/mL was shown for the color-indicating DDLC-bioinspired sensors. We also designed a new method to use the quantitative DDLC-bioinspired sensor with a smart-phone for potential of home test. The novel DDLC-bioinspired sensor is cheap, label-free, and easy to use, furthering the technology for home and field-based disease-related detection.

scholarly journals Label-Free Optical Detection of Mycotoxins Using Specific Aptamers Immobilized on Gold Nanostructures

Toxins ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 291 ◽  
Author(s):  
Ali Ghanim Al-Rubaye ◽  
Alexei Nabok ◽  
Gaelle Catanante ◽  
Jean-Louis Marty ◽  
Eszter Takács ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Localized Surface Plasmon ◽  
The Novel ◽  
Label Free ◽  
Binding Characteristics ◽  
Highly Sensitive ◽  
Wide Range ◽  
Dna Chain ◽  
First Time ◽  
Kinetics Of

This work focuses on the development of the novel label-free optical apta-sensors for detection of mycotoxins. A highly sensitive analytical method of total internal reflection ellipsometry (TIRE) combined with Localized Surface Plasmon Resonance (LSPR) phenomenon in nano-structured gold films was exploited here for the first time for detection of aflatoxin B1 and M1 in direct assay with specific aptamers immobilized on the surface of gold. The achieved detection of low molecular weight molecules, such as aflatoxin B1 and M1, in a wide range of concentrations from 100 ng/mL down to 0.01 ng/mL is remarkable for the LSPR method. The study of binding kinetics of aflatoxin molecules to their respective aptamers using dynamic TIRE measurements yielded the values of affinity constants in the range of 10−8–10−7 mol, which is characteristic for highly specific aptamer/target interactions similar to that for monoclonal antibodies. The effect of aptamers’ DNA chain length on their binding characteristics was analyzed.

scholarly journals Label-Free, Color-Indicating, Polarizer-Free Dye-Doped Liquid Crystal Microfluidic Polydimethylsiloxane Biosensing Chips for Detecting Albumin

Polymers ◽  
2021 ◽  
Vol 13 (16) ◽  
pp. 2587
Author(s):  
Fu-Lun Chen ◽  
Hui-Tzung Luh ◽  
Yu-Cheng Hsiao
Keyword(s):  
Liquid Crystal ◽  
Immune Responses ◽  
The Novel ◽  
Microfluidic Chips ◽  
Label Free ◽  
Sequential Order ◽  
Alignment Layer ◽  
Polarized Microscopy ◽  
Pressure Driven Flow ◽  
Novel Design

We reveal a novel design for dye-doped liquid crystal (DDLC) microfluidic biosensing chips in the polydimethylsiloxane material. With this design chip, the orientation of DDLCs was affected by the interface between the walls of the channels and DDLCs. When the inside of a channel was coated with an N,N-dimethyl-n-octadecyl-3-aminopropyltrimethoxysilyl chloride (DMOAP) alignment layer, the DDLCs oriented homeotropically in a homeotropic (H) state under cross-polarized microscopy. After immobilization of antigens with antibodies on the alignment layer-coated microchannel walls, the optical intensity of the DDLC change from the dark H state to the bright planar (P) state. Using pressure-driven flow, the binding of antigens/antibodies to the DDLCs could be detected in an experimental sequential order. The microfluidic DDLCs were tested by detecting bovine serum albumin (BSA) and its immune-responses of antigens/antibodies. We proved that this immunoassay chip was able to detect BSA antigens/antibodies pairs with the detection limit about 0.5 µg/mL. The novel DDLC chip was shown to be a simple, multi-detection device, and label-free microfluidic chips are presented.

scholarly journals Optical Monitoring of the Production Quality of Si-Nanoribbon Chips Intended for the Detection of ASD-Associated Oligonucleotides

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 147
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Vladimir P. Popov ◽  
Igor N. Kupriyanov ◽  
Rafael A. Galiullin ◽  
...  
Keyword(s):  
Production Quality ◽  
Autism Spectrum ◽  
Covalent Immobilization ◽  
Biological Macromolecules ◽  
Label Free ◽  
Raman Scattering Spectroscopy ◽  
Dna Oligonucleotides ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Gas-phase etching and optical lithography were employed for the fabrication of a silicon nanoribbon chip (Si-NR chip). The quality of the so-fabricated silicon nanoribbons (Si-NRs) was monitored by optical Raman scattering spectroscopy. It was demonstrated that the structures of the Si-NRs were virtually defect-free, meaning they could be used for highly sensitive detection of biological macromolecules. The Si-NR chips were then used for the highly sensitive nanoelectronics detection of DNA oligonucleotides (oDNAs), which represent synthetic analogs of 106a-5p microRNA (miR-106a-5p), associated with the development of autism spectrum disorders in children. The specificity of the analysis was attained by the sensitization of the Si-NR chip sur-face by covalent immobilization of oDNA probes, whose nucleotide sequence was complementary to the known sequence of miR-106a-5p. The use of the Si-NR chip was demonstrated to al-low for the rapid label-free real-time detection of oDNA at ultra-low (~10−17 M) concentrations.

Label-Free Highly Sensitive Hybrid Plasmonic Biosensor for the Detection of DNA Hybridization

2017 ◽  
Vol 35 (22) ◽  
pp. 4851-4858 ◽  
Author(s):  
Mohamed Farhat O. Hameed ◽  
Ahmed Samy Saadeldin ◽  
Essam M. A. Elkaramany ◽  
Salah S. A. Obayya
Keyword(s):  
Dna Hybridization ◽  
Label Free ◽  
Highly Sensitive
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