scholarly journals Different Effects of Albumin and Hydroxyethyl Starch on Low Molecular-Weight Solute Permeation through Sodium Hyaluronic Acid Solution

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 514
Author(s):  
Tsuneo Tatara
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Hydroxyethyl Starch ◽  
Interstitial Space ◽  
Colloid Solution ◽  
Cancer Tissue ◽  
Control Drug ◽  
Orange G ◽  
Control Drug Delivery ◽  
Selection Of

Hyaluronic acid (HA), a high-molecular-weight linear polysaccharide, restricts solute transport through the interstitial space. Albumin and hydroxyethyl starch (HES) solutions are used to correct the decrease of blood volume during surgery, but may leak into the interstitial space under inflammation conditions. Given the possibility that the structure of HA may be affected by adjacent macromolecules, this study tested whether albumin and HES (Mw 130,000) exert different effects on solute permeation through sodium hyaluronic acid (NaHA: Mw 1.3 × 106) solution. To this end, permeation of Orange G, a synthetic azo dye (Mw 452), into NaHA solutions containing albumin or HES over time was assessed. The amount of time it took for the relative absorbance of Orange G to reach 0.3 (T0.3) was determined in each NaHA solution relative to the reference solution (i.e., colloid solution without NaHA). Relative T0.3 values of albumin were larger than those of HES for 0.1% NaHA solution (3.33 ± 0.69 vs. 1.16 ± 0.08, p = 0.006, n = 3) and 0.2% NaHA solution (1.95 ± 0.32 vs. 0.92 ± 0.27, p = 0.013, n = 3). This finding may help in the selection of an appropriate colloid solution to control drug delivery into the interstitial space of cancer tissue under inflammation conditions.

Selection of a Mutant Strain Streptococcus equi SH-109 and Optimization of Culture Conditions for the Production of High Molecular Weight Hyaluronic Acid

2014 ◽  
Vol 915-916 ◽  
pp. 909-912 ◽  
Author(s):  
Jian Cheng Feng ◽  
Rong Hu Zhang
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
High Molecular Weight ◽  
Culture Conditions ◽  
Culture Broth ◽  
Agitation Speed ◽  
Wild Type ◽  
Streptococcus Equi ◽  
Optimal Ph ◽  
Selection Of

A high molecular weight hyaluronic acid-producing mutant, designated asStreptococcus equiSH-109 that did not produce streptolysin or hyaluronidase was selected from wild-typeStreptococcus equiSH-0 by NTG treatment. The selected mutant could produce high molecular weight HA and had perfect genetic stability after reproduction. Several culture conditions were optimized in a 5-l fermentor for the production of hyaluronic acid. It was found that the optimal pH and agitation speed were 7.6 and 550rpm, respectively. Under the optimized culture condition, hyaluronic acid with a molecular weight of 4.0 x 106was accumulated in the culture broth.

scholarly journals Fabrication of Pure Electrospun Materials from Hyaluronic Acid

2017 ◽  
Vol 25 (0) ◽  
pp. 45-52 ◽  
Author(s):  
Ewelina Pabjańczyk-Wlazło ◽  
Izabella Krucińska ◽  
Michał Chrzanowski ◽  
Grzegorz Szparaga ◽  
Agata Chaberska ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
Process Parameters ◽  
Solvent Mixture ◽  
Electrospinning Process ◽  
Optimal Conditions ◽  
Variable Parameters ◽  
Three Stages ◽  
Solvent Residue ◽  
Selection Of

The aim of the research was to develop optimal conditions for manufacturing materials based on hyaluronic acid by the electrospun method. The studies were composed of three stages: the process of selection of the optimal solvent (mixture of solvents), the molecular weight of hyaluronic acid, and the concentration of biopolymer in the spinning solution. The influence of variable parameters on the rheological properties of the spinning solutions and electrospinning trails was tested. Depending on the electrospinning regime applied, the fibers obtained were characterised by a diameter of the order of 20 to 400 nm. As a result of the development works presented, an optimal molecular weight of the polymer, its concentration and system of solvents were determined, together with process parameters, ensuring a stable electrospinning process and relatively homogeneous nanofibers. Additionally studies on the residues of solvents used during electrosun formation were done and parameters of drying of the final materials were examined. This approach (verification of the presence oforganic solvent residue in the nanofibrous formed) is important for the suitability of nanofibres as scaffolds for regenerative medicine. This study provides an opportunity for the understanding and identification of process parameters, allowing for predictable manufacturing nanofibers based on natural biopolymers, which makes it tremendously beneficial in terms of customisation.

HES 200/0.5 Is not HES 200/0.5

1995 ◽  
Vol 74 (06) ◽  
pp. 1452-1456 ◽  
Author(s):  
Johannes Treib ◽  
Anton Haass ◽  
Gerhard Pindur ◽  
Ulrich T Seyfert ◽  
Wolfgang Treib ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hydroxyethyl Starch ◽  
Clinical Relevance ◽  
Dilution Effect ◽  
Repeated Administration ◽  
Plasma Viscosity ◽  
Degree Of Substitution ◽  
Pronounced Increase ◽  
Large Molecules ◽  
Mean Molecular Weight

SummaryThe plasma clearance of hydroxyethyl starch (HES) depends on the initial molecular weight and the degree of substitution. So far, little attention has been paid to the clinical relevance of the C2/C6 substitution ratio of hydroxyethyl starch.10 patients with cerebrovascular circulatory disturbance received hemodilution therapy for 10 days, consisting of 10% HES 200/0.5 (mean molecular weight 200 kD, degree of substitution 0.5) with a C2/C6 ratio of 13.4. A second group of 10 patients received a starch solution with identical initial molecular weight and degree of substitution but with a C2/C6 ratio of 5.7.After the administration of a single dose, no significant differences between the two groups were observed. After repeated administration, significant differences could be detected in hemorheology, coagulation and elimination (p<0.01). The larger C2/C6 ratio led to a higher intravascular mean molecular weight (95 vs. 84 kD), which in turn led to a higher increase in serum concentration during the therapy (14.7 vs.8.6 mg/ml). Hematocrit was lowered more (-30,5 vs. -23,5%) and plasma viscosity was increased more. There was also a more pronounced increase in partial thromboplastin time (+30% vs. +13%) and a factor of 2 larger decrease of factor VIII/von Willebrand factor-complex (p <0.01), which exceeded the dilution effect.The higher C2/C6 ratio of HES 200/0.5/13.4 slows down enzymatic degradation. After repeated administration of this starch, large molecules accumulate which are inefficiently degraded. The same effect has been observed after therapy with highly-substituted HES. This accumulation of large molecules leads to a beneficial longer lasting volume effect. The disadvantages include an increase in plasma viscosity and coagulation disturbances, which cannot be explained with the respective dilution effect alone. For these reasons, the C2/C6 ratio is of clinical relevance and should be included in the product labeling in the future.

Preparation and Characterization of the High Molecular Weight [3H]Hyaluronic Acid

1992 ◽  
Vol 57 (10) ◽  
pp. 2151-2156 ◽  
Author(s):  
Peter Chabreček ◽  
Ladislav Šoltés ◽  
Hynek Hradec ◽  
Jiří Filip ◽  
Eduard Orviský
Keyword(s):  
Molecular Weight ◽  
Hyaluronic Acid ◽  
High Molecular Weight ◽  
Methyl Bromide ◽  
High Performance ◽  
Hydrogen Atoms ◽  
Isolation And Characterization ◽  
Labelling Procedure ◽  
Enzymatic Depolymerization

Two methods for the preparation of high molecular weight [3H]hyaluronic acid were investigated. In the first one, hydrogen atoms in the molecule were replaced by tritium. This isotopic substitution was performed in aqueous solution using Pd/CaCO3 as the catalyst. In the second method, the high molecular weight hyaluronic acid was alkylated with [3H]methyl bromide in liquid ammonia at a temperature of -33.5 °C. High-performance gel permeation chromatographic separation method was used for the isolation and characterization of the high molecular weight [3H]hyaluronic acid. Molecular weight parameters for the labelled biopolymers were Mw = 128 kDa, Mw/Mn = 1.88 (first method) and Mw = 268 kDa, Mw/Mn = 1.55 (second method). The high molecular weight [3H]hyaluronic acid having Mw = 268 kDa was degraded further by specific hyaluronidase. Products of the enzymatic depolymerization were observed to be identical for both, labelled and cold biopolymer. This finding indicates that the described labelling procedure using [3H]methyl bromide does not induce any major structural rearrangements in the molecule.

scholarly journals Wound Healing Promotion by Hyaluronic Acid: Effect of Molecular Weight on Gene Expression and In Vivo Wound Closure

2021 ◽  
Vol 14 (4) ◽  
pp. 301
Author(s):  
Yayoi Kawano ◽  
Viorica Patrulea ◽  
Emmanuelle Sublet ◽  
Gerrit Borchard ◽  
Takuya Iyoda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Weight ◽  
Wound Healing ◽  
Hyaluronic Acid ◽  
Healing Process ◽  
Dermal Fibroblasts ◽  
Water Soluble ◽  
Wound Healing Process ◽  
And Migration ◽  
Proliferation And Migration

Hyaluronic acid (HA) has been known to play an important role in wound healing process. However, the effect of molecular weight (MW) of exogenously administered HA on the wound healing process has not been fully understood. In this study, we investigated HA with different MWs on wound healing process using human epidermal keratinocytes and dermal fibroblasts. Cell proliferation and migration ability were assessed by water soluble tetrazolium (WST) assay and wound scratch assay. We examined the effect of HA addition in a full-thickness wound model in mice and the gene expression related to wound healing. Proliferation and migration of HaCaT cells increased with the increase of MW and concentration of HA. Interleukin (IL-1β), IL-8 and vascular endothelial growth factor (VEGF) as well as matrix metalloproteinase (MMP)-9 and MMP-13 were significantly upregulated by high molecular weight (HMW) HA in keratinocytes. Together with VEGF upregulation and the observed promotion of HaCaT migration, HA with the MW of 2290 kDa may hold potential to improve re-epithelialization, a critical obstacle to heal chronic wounds.

Influence of intravascular molecular weight of hydroxyethyl starch on platelets

2009 ◽  
Vol 56 (3) ◽  
pp. 168-172 ◽  
Author(s):  
J. Treib ◽  
A. Haass ◽  
G. Pindur ◽  
W. Treib ◽  
E. Wenzel ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Hydroxyethyl Starch
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