scholarly journals The Effect of Different Cleaning Methods on Protein Deposition and Optical Characteristics of Orthokeratology Lenses

Polymers ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 4318
Author(s):  
Chen-Ying Su ◽  
Lung-Kun Yeh ◽  
Yi-Fei Tsao ◽  
Wen-Pin Lin ◽  
Chiun-Ho Hou ◽  
...  
Keyword(s):  
Optical Characteristics ◽  
Short Term ◽  
Protein Deposition ◽  
Tear Proteins ◽  
Cleaning Methods ◽  
Vitro Analysis ◽  
In Vitro Analysis ◽  
Myopia Control

Orthokeratology lenses are commonly used for myopia control, especially in children. Tear lipids and proteins are immediately adsorbed when the lens is put on the cornea, and protein deposition may cause discomfort or infection. Therefore, we established an in vitro protein deposition analysis by mimicking the current cleaning methods for orthokeratology lens wearers for both short-term and long-term period. The results showed that the amounts of tear proteins accumulated daily and achieved a balance after 14 days when the lens was rubbed to clean or not. Protein deposition also affected the optical characteristics of the lens regardless of cleaning methods. Our results provided an in vitro analysis for protein deposition on the lens, and they may provide a potential effective method for developing care solutions or methods that can more effectively remove tear components from orthokeratology lenses.

Long-term in vitro analysis of limb cartilage development: Involvement of Wnt signaling

2004 ◽  
Vol 93 (3) ◽  
pp. 526-541 ◽  
Author(s):  
Kathleen M. Daumer ◽  
A. Cevik Tufan ◽  
Rocky S. Tuan
Keyword(s):  
Wnt Signaling ◽  
Cartilage Development ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Cariogenic and erosive potential of liquid oral pediatric medicines of long-term use for children: an in vitro analysis

2020 ◽  
Author(s):  
Ola B. Al-Batayneh ◽  
Ala'a B. Al-Bataina ◽  
Yousef S. Khader ◽  
Arwa I. Owais
Keyword(s):  
Dental Caries ◽  
Chronic Administration ◽  
Low Ph ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Vitro Analysis ◽  
High Sucrose ◽  
In Vitro Analysis

Abstract Background Liquid oral pediatric medicines (LOPM) contain sucrose and glucose and can have a low pH; their chronic administration may increase the risk for dental caries and erosion in children. The aim of this study was to determine sucrose and glucose concentrations, and pH of LOPMs of long-term use by children. Methods A cross sectional survey was conducted among 95/381 pharmacies to assess the most commonly prescribed LOPM by pharmacists in North Jordan, yielding 42 medicines which were analyzed for sucrose and glucose concentrations (mg/g) using HPLC-RID, and pH measurement using pH-meter CP-505. Data were analyzed using SPSS version 19.0. Differences were considered significant at P ≤ 0.05. Results The lowest and highest sucrose concentrations were for Ramlac® (0.9%) from the gastrointestinal medicines and Varolex® (79.5%) from the nutritional medicines, respectively. The lowest and highest glucose concentrations were for Adol® (0.0%) from the central nervous medicines and Pediavit-D® (81.1%) from the nutritional medicines, respectively. The pH ranged from 3.2 for Varolex® from the nutritional medicines to 10.0 for Zithromax® from the antibiotics medicines. Conclusions Some of the LOPMs studied had high sucrose or glucose concentrations (or both) and low pH which could increase their potential of causing dental caries and erosion when ingested frequently and chronically.

The Significance of Surgery for Bulky N2 Small-Cell Lung Cancer: A Clinical and In Vitro Analysis of Long-Term Survivors

Surgery Today ◽  
2000 ◽  
Vol 30 (11) ◽  
pp. 978-986 ◽  
Author(s):  
Shunsuke Kobayashi ◽  
Shinichiro Okada ◽  
Toru Hasumi ◽  
Nobuyuki Sato ◽  
Shigefumi Fujimura
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Vitro Analysis ◽  
Bulky N2 ◽  
Long Term Survivors ◽  
In Vitro Analysis

scholarly journals Comparative short-term in vitro analysis of mutans streptococci adhesion on esthetic, nickel-titanium, and stainless-steel arch wires

2014 ◽  
Vol 84 (4) ◽  
pp. 680-686 ◽  
Author(s):  
In-Hye Kim ◽  
Hyo-Sang Park ◽  
Young Kyung Kim ◽  
Kyo-Han Kim ◽  
Tae-Yub Kwon
Keyword(s):  
Stainless Steel ◽  
Nickel Titanium ◽  
Mutans Streptococci ◽  
Short Term ◽  
Vitro Analysis ◽  
In Vitro Analysis

scholarly journals Crosstalk of Endothelial and Mesenchymal Stromal Cells under Tissue-Related O2

2021 ◽  
Vol 1 (2) ◽  
pp. 116-136
Author(s):  
Olga Zhidkova ◽  
Elena Andreeva ◽  
Mariia Ezdakova ◽  
Ludmila Buravkova
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Reciprocal Effects ◽  
Hypoxic Stress ◽  
Protective Effects ◽  
Short Term ◽  
Tnf Α ◽  
Vitro Analysis ◽  
In Vitro Analysis

Mesenchymal stromal cells (MSCs) are considered a valuable tool for cell therapy. After systemic administration, the outcome of MSCs and endothelial cells (ECs) interactions strongly depend on the local microenvironment and tissue O2 levels in particular. In vitro analysis of EC effects on MSC regenerative potential in co-culture was performed after short-term interaction at “physiological” hypoxia (5% O2) and acute hypoxic stress (0.1% O2). At 5% O2, MSCs retained stromal phenotype and CFU-f numbers, osteogenic RUNX2 was upregulated. A shift in the expression of adhesion molecules, and an increase in transcription/synthesis of IL-6, IL-8 contributed to facilitation of directed migration of MSCs. In the presence of MSCs, manifestations of oxidative stress in ECs were attenuated, and a decrease in adhesion of PBMCs to TNF-α-activated ECs was observed. Under 0.1% O2, reciprocal effects of ECs and MSCs were similar to those at 5% O2. Meanwhile, upregulation of RUNX2 was canceled, IL-6 decreased, and IL-8 significantly increased. “Protective” effects of MSCs on TNF-α-ECs were less pronounced, manifested as NOS3 downregulation and intracellular NO elevation. Therefore, interaction with ECs at “physiological” hypoxia enhanced pro-regenerative capacities of MSCs including migration and anti-inflammatory modulation of ECs. Under acute hypoxic stress, the stimulating effects of ECs on MSCs and the “protective” potential of MSCs towards TNF-α-ECs were attenuated.

In vitro analysis of the epstein-barr virus: Host balance in long-term renal allograft recipients

1985 ◽  
Vol 35 (1) ◽  
pp. 43-49 ◽  
Author(s):  
Q. Y. Yao ◽  
A. B. Rickinson ◽  
J. S. H. Gaston ◽  
M. A. Epstein
Keyword(s):  
Epstein Barr Virus ◽  
Renal Allograft ◽  
Barr Virus ◽  
Epstein Barr ◽  
Vitro Analysis ◽  
In Vitro Analysis
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