scholarly journals Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of Rhodococcus erythropolis IGTS8

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 2064
Author(s):  
George Prasoulas ◽  
Konstantinos Dimos ◽  
Panayiotis Glekas ◽  
Styliani Kalantzi ◽  
Stamatis Sarris ◽  
...  

Biodesulfurization (BDS) is considered a complementary technology to the traditional hydrodesulfurization treatment for the removal of recalcitrant sulfur compounds from petroleum products. BDS was investigated in a bubble column bioreactor using two-phase media. The effects of various process parameters, such as biocatalyst age and concentration, organic fraction percentage (OFP), and type of sulfur compound—namely, dibenzothiophene (DBT), 4-methyldibenzothiophene (4-MDBT), 4,6-dimethyldibenzothiophene (4,6-DMDBT), and 4,6-diethyldibenzothiophene (4,6-DEDBT)—were evaluated, using resting cells of Rhodococcus erythropolis IGTS8. Cells derived from the beginning of the exponential growth phase of the bacterium exhibited the highest biodesulfurization efficiency and rate. The biocatalyst performed better in an OFP of 50% v/v. The extent of DBT desulfurization was dependent on cell concentration, with the desulfurization rate reaching its maximum at intermediate cell concentrations. A new semi-empirical model for the biphasic BDS was developed, based on the overall Michaelis-Menten kinetics and taking into consideration the deactivation of the biocatalyst over time, as well as the underlying mass transfer phenomena. The model fitted experimental data on DBT consumption and 2-hydroxibyphenyl (2-HBP) accumulation in the organic phase for various initial DBT concentrations and different organosulfur compounds. For constant OFP and biocatalyst concentration, the most important parameter that affects BDS efficiency seems to be biocatalyst deactivation, while the phenomenon is controlled by the affinities of biodesulfurizing enzymes for the different organosulfur compounds. Thus, desulfurization efficiency decreased with increasing initial DBT concentration, and in inverse proportion to increases in the carbon number of alkyl substituent groups.

2014 ◽  
Vol 28 (12) ◽  
pp. 7552-7559 ◽  
Author(s):  
Jorge L. S. Sonego ◽  
Diego A. Lemos ◽  
Guilherme Y. Rodriguez ◽  
Antonio J. G. Cruz ◽  
Alberto C. Badino

2018 ◽  
Vol 334 ◽  
pp. 691-697 ◽  
Author(s):  
Teresa García-Pérez ◽  
Juan C. López ◽  
Fabiana Passos ◽  
Raquel Lebrero ◽  
Sergio Revah ◽  
...  

Author(s):  
K. Sandesh ◽  
P. Ujwal ◽  
Blecita D. Mascarenhas ◽  
Gayatri Dhamannavar ◽  
Narmada Kumar ◽  
...  

2019 ◽  
Vol 6 (3) ◽  
pp. 84 ◽  
Author(s):  
Moradi ◽  
Rashedi ◽  
Mofradnia ◽  
Khosravi-Darani ◽  
Ashouri ◽  
...  

In this study, the simulation of microorganism ability for the production of poly-β-hydroxybutyrate (PHB) from natural gas (as a carbon source) was carried out. Based on the Taguchi algorithm, the optimum situations for PHB production from natural gas in the columnar bubble reactor with 30 cm length and 1.5 cm diameter at a temperature of 32 °C was evaluated. So, the volume ratio of air to methane of 50:50 was calculated. The simulation was carried out by COMSOL software with two-dimensional symmetric mode. Mass transfer, momentum, density-time, and density-place were investigated. The maximum production of biomass concentration reached was 1.63 g/L, which shows a 10% difference in contrast to the number of experimental results. Furthermore, the consequence of inlet gas rate on concentration and gas hold up was investigated Andres the simulation results were confirmed to experimental results with less than 20% error.


Sign in / Sign up

Export Citation Format

Share Document