Pharmacokinetics of Chiral Dendrimer-Triamine-Coordinated Gd-MRI Contrast Agents Evaluated by in Vivo MRI and Estimated by in Vitro QCM

The purpose of this study is to establish the minimal injection doses of magnetic resonance imaging (MRI) contrast agents that can achieve optimized images while improving the safety of injectable MRI drugs. Gadolinium-diethylenetriamine penta-acetic acid (Gd-DTPA) and ferucarbotran, commonly used in clinical practice, were selected and evaluated with in vitro and in vivo experiments. MRI was acquired using T1-weighted (T1W) and T2-weighted (T2W) sequences, and the results were quantitatively analyzed. For in vitro experiments, results showed that T1W and T2W images were optimal when Gd-DTPA-bisamide (2-oxoethyl) (Gd-DTPA-BMEA) and ferucarbotran were diluted to a volume percentage of 0.6% and 0.05%; all comparisons were significant differences in grayscale statistics using one-way analysis of variance (ANOVA). For in vivo experiments, the contrast agent with optimal concentration percentages determined from in vitro experiments were injected into mice with an injection volume of 100 μL, and the images of brain, heart, liver, and mesentery before and after injection were compared. The statistical results showed that the p values of both T1W and T2W were less than 0.001, which were statistically significant. Under safety considerations for MRI contrast agent injection, optimized MRI images could still be obtained after reducing the injection concentration, which can provide a reference for the safety concentrations of MRI contrast agent injection in the future.

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Genetic encoding of targeted MRI contrast agents for in vivo tumor imaging

10.1101/799411 ◽

2019 ◽

Author(s):

Simone Schuerle ◽

Maiko Furubayashi ◽

Ava P. Soleimany ◽

Tinotenda Gwisai ◽

Wei Huang ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Contrast Agents ◽

Tumor Imaging ◽

Mri Contrast Agents ◽

Contrast Effects ◽

Mri Contrast ◽

Magnetic Resonance Imaging Mri ◽

Targeted Mri

AbstractTumor-selective contrast agents have the potential to aid in the diagnosis and treatment of cancer using noninvasive imaging modalities such as magnetic resonance imaging (MRI). Such contrast agents can consist of magnetic nanoparticles incorporating functionalities that respond to cues specific to tumor environments. Genetically engineering magnetotactic bacteria to display peptides has been investigated as a means to produce contrast agents that combine the robust image contrast effects of magnetosomes with transgenic targeting peptides displayed on their surface. This work reports the first use of magnetic nanoparticles that display genetically-encoded pH low insertion peptide (pHLIP), a long peptide intended to enhance MRI contrast by targeting the extracellular acidity associated with the tumors. To demonstrate the modularity of this versatile platform to incorporate diverse targeting ligands by genetic engineering, we also incorporated the cyclic αv integrin-binding peptide iRGD into separate magnetosomes. Specifically, we investigate their potential for enhanced binding and tumor imaging both in vitro and in vivo. Our experiments indicate that these tailored magnetosomes retain their magnetic properties, making them well-suited as T2 contrast agents, while exhibiting increased binding compared to wild-type magnetosomes.

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Preparation and Evaluation of Novel Sugar Dendritic Gd-DTPA Complexes for MRI Contrast Agents and Phospha Sugars for Anti-Tumour Agents

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.222.217 ◽

2011 ◽

Vol 222 ◽

pp. 217-220

Author(s):

Keita Kiyofuji ◽

Kenji Tsunekawa ◽

Mitsuji Yamashita ◽

Junko Yamashita ◽

Michio Fujie ◽

...

Keyword(s):

Contrast Agents ◽

Leukemia Cell ◽

Mri Contrast Agents ◽

Solid Cancer ◽

Mri Contrast ◽

Liver Cancers ◽

Heterocyclic Derivatives ◽

Sugar Derivatives

Novel Sugar Dendritic Gd(III)-DTPA complexes for MRI Contrast Agents (CAs) were prepared and evaluated by in vitro and in vivo methods. The sugar dendritic MRI contrast agents had a good blood vessel pool character and drew blood vessels and liver cancers remarkably clearer and longer time enough than the clinically being used Gd(III)-DTPA complex (Magnevist). Phospha sugar derivatives or phosphorus heterocyclic derivatives provided by functional groups such as epoxide, bromide, etc., were prepared and evaluated by the MTT in vitro method. These phospha sugar derivatives showed excellent anti-proliferative effects of leukemia cell lines, e.g., K562 and U937, as well as solid cancer cells in fashions of (i) higher activity, (ii) wider spectra, and (iii) higher selectivity and specificity than Imatinib mesylate (Gleevec), which is one of the most frequently used chemotherapeutical molecular targeting anti-tumour agent.

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In Vitro and In Vivo Studies of Trimetallic Nitride Endohedral Metallofullerene MRI Contrast Agents

ECS Meeting Abstracts ◽

10.1149/ma2005-01/26/934 ◽

2005 ◽

Keyword(s):

Contrast Agents ◽

Mri Contrast Agents ◽

In Vivo Studies ◽

Mri Contrast ◽

Endohedral Metallofullerene

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Water-soluble magnetic CoO nanocrystals functionalized with surfactants as T2-weighed MRI contrast agents in vitro

Dalton Transactions ◽

10.1039/c1dt10107b ◽

2011 ◽

Vol 40 (14) ◽

pp. 3616 ◽

Cited By ~ 14

Author(s):

Hong Yang ◽

Hong Zhou ◽

Cuixia Zhang ◽

Xuejian Li ◽

He Hu ◽

...

Keyword(s):

Contrast Agents ◽

Mri Contrast Agents ◽

Water Soluble ◽

Mri Contrast

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Dynamic, Simultaneous Concentration Mapping of Multiple MRI Contrast Agents with Dual Contrast - Magnetic Resonance Fingerprinting

Scientific Reports ◽

10.1038/s41598-019-56531-7 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Christian E. Anderson ◽

Mette Johansen ◽

Bernadette O. Erokwu ◽

He Hu ◽

Yuning Gu ◽

...

Keyword(s):

Magnetic Resonance ◽

Contrast Agents ◽

Ex Vivo ◽

Single Agent ◽

Mri Contrast Agents ◽

Mri Contrast Agent ◽

Spatiotemporal Resolution ◽

Mri Contrast ◽

Contrast Magnetic Resonance

AbstractSynchronous assessment of multiple MRI contrast agents in a single scanning session would provide a new “multi-color” imaging capability similar to fluorescence imaging but with high spatiotemporal resolution and unlimited imaging depth. This multi-agent MRI technology would enable a whole new class of basic science and clinical MRI experiments that simultaneously explore multiple physiologic/molecular events in vivo. Unfortunately, conventional MRI acquisition techniques are only capable of detecting and quantifying one paramagnetic MRI contrast agent at a time. Herein, the Dual Contrast – Magnetic Resonance Fingerprinting (DC-MRF) methodology was extended for in vivo application and evaluated by simultaneously and dynamically mapping the intra-tumoral concentration of two MRI contrast agents (Gd-BOPTA and Dy-DOTA-azide) in a mouse glioma model. Co-registered gadolinium and dysprosium concentration maps were generated with sub-millimeter spatial resolution and acquired dynamically with just over 2-minute temporal resolution. Mean tumor Gd and Dy concentration measurements from both single agent and dual agent DC-MRF studies demonstrated significant correlations with ex vivo mass spectrometry elemental analyses. This initial in vivo study demonstrates the potential for DC-MRF to provide a useful dual-agent MRI platform.

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LDL-mimetic lipid nanoparticles prepared by surface KAT ligation for in vivo MRI of atherosclerosis

Chemical Science ◽

10.1039/d0sc04106h ◽

2020 ◽

Vol 11 (44) ◽

pp. 11998-12008

Author(s):

Alessandro Fracassi ◽

Jianbo Cao ◽

Naoko Yoshizawa-Sugata ◽

Éva Tóth ◽

Corey Archer ◽

...

Keyword(s):

Contrast Agents ◽

Fluorescent Dyes ◽

Lipid Nanoparticles ◽

Mri Contrast Agents ◽

Mri Contrast ◽

Mimetic Peptide

LDL-mimetic lipid nanoparticles, decorated with MRI contrast agents and fluorescent dyes, were prepared by the covalent attachments of an apoB100-mimetic peptide, Gd(iii)-chelate, and rhodamine to enhance atherosclerosis in the in vivo imaging.

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Uniform Fe3O4/Gd2O3-DHCA nanocubes for dual-mode magnetic resonance imaging

Beilstein Journal of Nanotechnology ◽

10.3762/bjnano.11.84 ◽

2020 ◽

Vol 11 ◽

pp. 1000-1009

Author(s):

Miao Qin ◽

Yueyou Peng ◽

Mengjie Xu ◽

Hui Yan ◽

Yizhu Cheng ◽

...

Keyword(s):

Magnetic Resonance Imaging ◽

Magnetic Resonance ◽

Contrast Agents ◽

Mri Contrast Agents ◽

Clinical Diagnostics ◽

Resonance Imaging ◽

Dual Mode ◽

Mri Contrast ◽

Magnetic Resonance Imaging Mri

The multimodal magnetic resonance imaging (MRI) technique has been extensively studied over the past few years since it offers complementary information that can increase diagnostic accuracy. Simple methods to synthesize contrast agents are necessary for the development of multimodal MRI. Herein, uniformly distributed Fe3O4/Gd2O3 nanocubes for T 1–T 2 dual-mode MRI contrast agents were successfully designed and synthesized. In order to increase hydrophilicity and biocompatibility, the nanocubes were coated with nontoxic 3,4-dihydroxyhydrocinnamic acid (DHCA). The results show that iron (Fe) and gadolinium (Gd) were homogeneously distributed throughout the Fe3O4/Gd2O3-DHCA (FGDA) nanocubes. Relaxation time analysis was performed on the images obtained from the 3.0 T scanner. The results demonstrated that r 1 and r 2 maximum values were 67.57 ± 6.2 and 24.2 ± 1.46 mM−1·s−1, respectively. In vivo T 1- and T 2-weighted images showed that FGDA nanocubes act as a dual-mode contrast agent enhancing MRI quality. Overall, these experimental results suggest that the FGDA nanocubes are interesting tools that can be used to increase MRI quality, enabling accurate clinical diagnostics.

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