scholarly journals Inkjet-Printed and Electroplated 3D Electrodes for Recording Extracellular Signals in Cell Culture

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3981
Author(s):  
Leroy Grob ◽  
Philipp Rinklin ◽  
Sabine Zips ◽  
Dirk Mayer ◽  
Sabrina Weidlich ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microelectrode Arrays ◽  
Silver Ions ◽  
Extracellular Signals ◽  
3D Electrodes ◽  
Inkjet Printer ◽  
Nanoparticle Ink ◽  
Cellular Medium ◽  
Extracellular Potentials ◽  
Printing Method

Recent investigations into cardiac or nervous tissues call for systems that are able to electrically record in 3D as opposed to 2D. Typically, challenging microfabrication steps are required to produce 3D microelectrode arrays capable of recording at the desired position within the tissue of interest. As an alternative, additive manufacturing is becoming a versatile platform for rapidly prototyping novel sensors with flexible geometric design. In this work, 3D MEAs for cell-culture applications were fabricated using a piezoelectric inkjet printer. The aspect ratio and height of the printed 3D electrodes were user-defined by adjusting the number of deposited droplets of silver nanoparticle ink along with a continuous printing method and an appropriate drop-to-drop delay. The Ag 3D MEAs were later electroplated with Au and Pt in order to reduce leakage of potentially cytotoxic silver ions into the cellular medium. The functionality of the array was confirmed using impedance spectroscopy, cyclic voltammetry, and recordings of extracellular potentials from cardiomyocyte-like HL-1 cells.

scholarly journals The variable toxicity of silver ions in cell culture media

2019 ◽  
Vol 60 ◽  
pp. 154-159 ◽  
Author(s):  
Paul Souter ◽  
Jim C. Cunningham ◽  
Alan Horner ◽  
Paul G. Genever
Keyword(s):  
Cell Culture ◽  
Culture Media ◽  
Silver Ions ◽  
Cell Culture Media

scholarly journals Deposition of PEDOT: PSS Nanoparticles as a Conductive Microlayer Anode in OLEDs Device by Desktop Inkjet Printer

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
S. Ummartyotin ◽  
J. Juntaro ◽  
C. Wu ◽  
M. Sain ◽  
H. Manuspiya
Keyword(s):  
Particle Size ◽  
Sulfonic Acid ◽  
Smooth Surface ◽  
Microelectrode Arrays ◽  
Electrical Measurement ◽  
Statistical Average ◽  
Atomic Force ◽  
Polystyrene Sulfonic Acid ◽  
Microfabrication Technique ◽  
Inkjet Printer

A simple microfabrication technique for delivering macromolecules and patterning microelectrode arrays using desktop inkjet printer was described. Aqueous solution of nanoparticle of poly (3,4-ethylenedioxythiophene) (PEDOT) doped with polystyrene sulfonic acid (PSS) was prepared while its particle size, the surface tension, and the viscosity of the solution were adjusted to be suitable for deposition on a flexible cellulose nanocomposite substrate via inkjet printer. The statistical average of PEDOT: PSS particle size of 100 nm was observed. The microthickness, surface morphology, and electrical conductivity of the printed substrate were then characterized by profilometer, atomic force microscope (AFM), and four-point probe electrical measurement, respectively. The inkjet deposition of PEDOT: PSS was successfully carried out, whilst retained its transparency feature. Highly smooth surface (roughness ~23–44 nm) was achieved.

PEDOT:PSS microelectrode arrays for hippocampal cell culture electrophysiological recordings

2017 ◽  
Vol 7 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Dimitrios A. Koutsouras ◽  
Adel Hama ◽  
Jolien Pas ◽  
Paschalis Gkoupidenis ◽  
Bruno Hivert ◽  
...  
Keyword(s):  
Cell Culture ◽  
Microelectrode Arrays ◽  
Hippocampal Cell ◽  
Electrophysiological Recordings ◽  
Hippocampal Cell Culture ◽  
Image Position

Abstract

scholarly journals Preparation of biological samples for the recording of electrophysiological signals using high-density microelectrode arrays

2020 ◽  
Author(s):  
Xinyue Yuan ◽  
Manuel Schröter ◽  
Marie Engelene J. Obien ◽  
Michele Fiscella ◽  
Wei Gong ◽  
...  
Keyword(s):  
Biological Samples ◽  
Cortical Neurons ◽  
Brain Slices ◽  
Microelectrode Arrays ◽  
High Density ◽  
Brain Functions ◽  
Extracellular Signals ◽  
Electrophysiological Signals ◽  
Electrophysiological Studies ◽  
Induced Pluripotent

Abstract The use of high-density microelectrode arrays (HD-MEAs) provides a promising approach for electrophysiological studies targeted at understanding of brain functions, profiling of neurodegenerative diseases, and drug screening. Here we present the protocol for the preparation of various biological samples for the recording of extracellular signals using HD-MEA, including primary cortical neurons, induced pluripotent stem cells (iPSCs)-derived neurons, rodent brain slices, retina, and iPSC-derived neuronal spheroids.

Characterization of surface modification on microelectrode arrays for in vitro cell culture

2007 ◽  
Vol 10 (1) ◽  
pp. 99-111 ◽  
Author(s):  
Shu-Ping Lin ◽  
Jia-Jin J. Chen ◽  
Jiunn-Der Liao ◽  
Shun-Fen Tzeng
Keyword(s):  
Cell Culture ◽  
Surface Modification ◽  
Microelectrode Arrays ◽  
In Vitro Cell Culture

scholarly journals Kinetics of growth of Leishmania (Leishmania) chagasi cycle in McCoy cell culture

2006 ◽  
Vol 48 (6) ◽  
pp. 337-341 ◽  
Author(s):  
Yeda L. Nogueira ◽  
Paulo M. Nakamura ◽  
Eunice A. B. Galati
Keyword(s):  
Cell Culture ◽  
Kinetic Analysis ◽  
Cell Lineage ◽  
Human Macrophage ◽  
Leishmania Chagasi ◽  
Sds Page ◽  
Promastigote Form ◽  
Kinetics Of ◽  
Cellular Medium

The kinetics of growth of Leishmania performed in vitro after internalization of the promastigote form in the cell and the occurrence of the transformation of the parasite into the amastigote form have been described by several authors. They used explants of macrophages in hamster spleen cell culture or in a human macrophage lineage cell, the U937. Using microscopy, the description of morphologic inter-relationship and the analysis of the production of specific molecules, it has been possible to define some of the peculiarities of the biology of the parasite. The present study shows the growth cycle of Leishmania chagasi during the observation of kinetic analysis undertaken with a McCoy cell lineage that lasted for a period of 144 hours. During the process, the morphologic transformation was revealed by indirect immunofluorescence (IF) and the molecules liberated in the extra cellular medium were observed by SDS-PAGE at 24-hour intervals during the whole 144-hour period. It was observed that in the first 72 hours the promastigote form of L. chagasi adhered to the cell membranes and assumed a rounded (amastigote-like) form. At 96 hours the infected cells showed morphologic alterations; at 120 hours the cells had liberated soluble fluorescent antigens into the extra cellular medium. At 144 hours, new elongated forms of the parasites, similar to promastigotes, were observed. In the SDS-PAGE, specific molecular weight proteins were observed at each point of the kinetic analysis showing that the McCoy cell imitates the macrophage and may be considered a useful model for the study of the infection of the Leishmania/cell binomial.

Developing an inkjet printer III: Multibit CMY halftones to hardware-ready bits

2020 ◽  
Vol 2020 (15) ◽  
pp. 352-1-352-9
Author(s):  
Sige Hu ◽  
Daulet Kenzhebalin ◽  
Bakedu Choi ◽  
George Chiu ◽  
Zillion Lin ◽  
...  
Keyword(s):  
Digital Image ◽  
Halftone Image ◽  
The World ◽  
Inkjet Printers ◽  
Inkjet Printer ◽  
Printing Method

Nowadays, inkjet printers are widely used all around the world. But how do they transfer the digital image to a map that can control nozzle firing? In this paper, we briefly illustrate that part of the printing pipeline that starts from a halftone image and end with Hardware Ready Bits (HRBs). We also describe the implementation of the multi-pass printing method with a designed print mask. HRBs are used to read an input halftone CMY image and output a binary map of each color to decide whether or not to eject the corresponding color drop at each pixel position. In general, for an inkjet printer, each row of the image corresponds to one specific nozzle in each swath so that each swath will be the height of the printhead [1]. To avoid visible white streaks due to clogged or burned out color nozzles, the method called multi-pass printing is implemented. Subsequently, the print mask is introduced so that we can decide during which pass each pixel should be printed.

A T.E.M. study of mouse mammary epithelial cell secretory differentiation cultured on collagen and Matrigel

1991 ◽  
Vol 49 ◽  
pp. 188-189
Author(s):  
W.N. Bentham ◽  
V. Rocha
Keyword(s):  
Cell Culture ◽  
Mammary Epithelial ◽  
Secretory Process ◽  
Cell Culture System ◽  
Protein Maturation ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Mouse Mammary Epithelial Cell ◽  
Secretory Differentiation

It has been an interest of our lab to develop a mammary epethelial cell culture system that faithfully duplicates the in vivo condition of the lactating gland. Since the introduction of collagen as a matrix on which cells are cultivated other E.C.M. type matrices have been made available and are used in many cell culture techniques. We have previously demonstrated that cells cultured on collagen and Matrigel do not differentiate as they do in vivo. It seems that these cultures often produce cells that show a disruption in the secretory process. The appearance of large ribosomal studded vesicles, that specifically label with antibody to casein, suggest an interruption of both protein maturation and secretion at the E.R. to golgi transition. In this report we have examined cultures on collagen and Matrigel at relative high and low seeding densities and compared them to cells from the in vivo condition.

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