scholarly journals A Proof-of-Concept Study Using Numerical Simulations of an Acoustic Spheroid-on-a-Chip Platform for Improving 3D Cell Culture

Sensors ◽  
2021 ◽  
Vol 21 (16) ◽  
pp. 5529
Author(s):  
Arash Yahyazadeh Shourabi ◽  
Roozbeh Salajeghe ◽  
Maryam Barisam ◽  
Navid Kashaninejad
Keyword(s):  
Flow Rate ◽  
Cultured Cells ◽  
3D Cell Culture ◽  
Potential Candidate ◽  
Proof Of Concept ◽  
Multicellular Spheroids ◽  
Lab On Chip ◽  
Main Challenge ◽  
Biological Studies ◽  
On Chip

Microfluidic lab-on-chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and quiescent zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. It will be shown numerically that such an approach is a potential candidate to be implemented to enhance cell viability and shrinks necrotic and quiescent zones without the need to increase the flow rate, leading to a significant reduction in costly reagents’ consumption in conventional spheroid-on-a-chip platforms. Proof-of-concept, designing procedures and numerical simulation are discussed in detail. Additionally, the effects of acoustic and hydrodynamic parameters on the cultured cells are investigated. The results show that by increasing acoustic boundary displacement amplitude (d0), the spheroid’s proliferating zone enlarges greatly. Moreover, it is shown that by implementing d0  = 0.5 nm, the required flow rate to maintain the necrotic zone below 13% will be decreased 12 times compared to non-acoustic chips.

scholarly journals An Acoustic Spheroid-on-Chip Platform for Long-Term Culturing of 3D Cell Aggregates

2021 ◽  
Author(s):  
Arash Yahyazadeh Shourabi ◽  
Roozbeh Salajeghe ◽  
Maryam Barisam ◽  
Navid Kashaninejad
Keyword(s):  
Flow Rate ◽  
Cultured Cells ◽  
Proof Of Concept ◽  
Multicellular Spheroids ◽  
Lab On Chip ◽  
Main Challenge ◽  
Biological Studies ◽  
Finite Element Numerical Simulation ◽  
On Chip

Microfluidic lab-on-chip devices are widely being developed for chemical and biological studies. One of the most commonly used types of these chips is perfusion microwells for culturing multicellular spheroids. The main challenge in such systems is the formation of substantial necrotic and hypoxic zones within the cultured spheroids. Herein, we propose a novel acoustofluidic integrated platform to tackle this bottleneck problem. We show that such an approach enhances cell viability and shrinks necrotic and hypoxic zones in these spheroid-on-a-chip platforms without the need to increase the flow rate, leading to a significant reduction in costly reagents' consumption. Proof-of-concept, designing procedures, and finite element numerical simulation are discussed in details. Also, the effects of acoustic and hydrodynamic parameters on the cultured cells are investigated. The results show that by increasing acoustic boundary displacement amplitude (d0), the spheroid’s proliferating zone enlarges greatly. Moreover, it is shown that by implementing d0=0.5 nm, the required flow rate to maintain the necrotic zone below 13% will be decreased 12 times compared to non-acoustic chips.

scholarly journals Integrated Magnetohydrodynamic Pump with Magnetic Composite Substrate and Laser-Induced Graphene Electrodes

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1113
Author(s):  
Mohammed Asadullah Khan ◽  
Jürgen Kosel
Keyword(s):  
Magnetic Flux ◽  
Flow Rate ◽  
Magnetic Composite ◽  
Propulsive Force ◽  
Closed Channel ◽  
Lab On Chip ◽  
Composite Substrate ◽  
On Chip ◽  
High Level ◽  
Moving Parts

An integrated polymer-based magnetohydrodynamic (MHD) pump that can actuate saline fluids in closed-channel devices is presented. MHD pumps are attractive for lab-on-chip applications, due to their ability to provide high propulsive force without any moving parts. Unlike other MHD devices, a high level of integration is demonstrated by incorporating both laser-induced graphene (LIG) electrodes as well as a NdFeB magnetic-flux source in the NdFeB-polydimethylsiloxane permanent magnetic composite substrate. The effects of transferring the LIG film from polyimide to the magnetic composite substrate were studied. Operation of the integrated magneto hydrodynamic pump without disruptive bubbles was achieved. In the studied case, the pump produces a flow rate of 28.1 µL/min. while consuming ~1 mW power.

scholarly journals Proof-of-concept demonstration of free-form optics enhanced confocal Raman spectroscopy in combination with optofluidic lab-on-chip

2016 ◽  
Author(s):  
Qing Liu ◽  
Diane De Coster ◽  
Damien Loterie ◽  
Jürgen Van Erps ◽  
Michael Vervaeke ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Free Form ◽  
Proof Of Concept ◽  
Confocal Raman Spectroscopy ◽  
Lab On Chip ◽  
Confocal Raman ◽  
On Chip

Integrated Microfluidic Flow Sensor for LAB-oN-CHIP and PoINT-oF-CARE Applications

2020 ◽  
Vol 36 (4) ◽  
pp. 112-120
Author(s):  
A.V. Zverev ◽  
M. Andronik ◽  
V.V. Echeistov ◽  
Z.H. Issabayeva ◽  
O.S. Sorokina ◽  
...  
Keyword(s):  
Numerical Simulation ◽  
Flow Rate ◽  
Microfluidic Chip ◽  
Soft Lithography ◽  
Point Of Care ◽  
Flow Sensor ◽  
Oxide Semiconductor ◽  
Dead Volume ◽  
Lab On Chip ◽  
On Chip

The results of the development and manufacture of an integrated membrane-free sensor for the control of accurate dilution of liquid samples on the microfluidic chip are presented. The proposed type of devices is intended for direct precise measurements of liquid flow rate in microchannels of laboratories-on-chip, including point-of-care systems. The sensor topology was optimized based on the numerical simulation results and technological requirements. The main characteristic of the developed sensor is the lack of a membrane in the design while maintaining the sensitivity and accuracy of the device at the level of a commercial membrane analogue. The fully biocompatible sensor was manufactured using standard microelectronics and soft lithography technologies. In order to optimize the sensor design, 32 different topologies of the device were tested. The integration of the flow sensors on the chip allows to significantly reduce the dead volume of the hydrodynamic system and to control the amount of liquid entering the individual reservoirs of the microfluidic chip. The sensor occupies an area of (210 x 140) um2 in the channel and is characterized by a relative error of 5% in the flow rate range of 100-1000 ul/min. microfluidics, lab-on-chip, calorimetric flow sensor, thermoresistive sensor, numerical simulation, hydrodynamics, complementary metal-oxide-semiconductor, microtechnologies Devices were made at the BMSTU Nanofabrication Facility (FMN Laboratory, FMNS REC, ID 74300).

Azeotropic drying free [18F]FDG synthesis and its application to a lab-on-chip platform

2016 ◽  
Vol 52 (4) ◽  
pp. 729-732 ◽  
Author(s):  
S. Lindner ◽  
C. Rensch ◽  
S. Neubaur ◽  
M. Neumeier ◽  
R. Salvamoser ◽  
...  
Keyword(s):  
Proof Of Concept ◽  
Hardware Complexity ◽  
Lab On Chip ◽  
18F Fdg ◽  
On Chip

[18F]FDG was prepared using a cartridge-based drying technique for [18F]fluoride. The application to a lab-on-chip platform demonstrates a proof of concept towards reduced hardware complexity.

Study of Permissible Flow Rate and Mixing Efficiency of the Micromixer Devices

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
K Karthikeyan ◽  
L Sujatha
Keyword(s):  
Flow Rate ◽  
Low Cost ◽  
Microfluidic Channel ◽  
Low Flow ◽  
Mixing Efficiency ◽  
Flow Rates ◽  
Lab On Chip ◽  
Serpentine Channel ◽  
Mixing Behavior ◽  
On Chip

AbstractThis paper deals with design, simulation, fabrication, analysis of mixing efficiency and thin film bonding stability of the micromixer devices with different flow rates used for lab on chip applications. The objective of the present study is to achieve complete mixing with low flow rate and less pressure drop in low cost polymer microfluidic devices. This paper emphasis the design, simulation and fabrication of straight channel micromixer, serpentine channel micromixer with and without quadrant shaped grooves to study the mixing behavior by the effect of structural dimensions of the microfluidic channel at different flow rates. The designed micromixers were tested with varying rates of flow such as 1, 10, 25, 50, 75 and 100 µL/min.

scholarly journals Rapid prototyping of a multilayer microphysiological system for primary human intestinal epithelial culture

2018 ◽  
Author(s):  
Sanjin Hosic ◽  
Marissa L. Puzan ◽  
Will Lake ◽  
Fanny Zhou ◽  
Ryan A. Koppes ◽  
...  
Keyword(s):  
3D Cell Culture ◽  
Water Vapor Permeability ◽  
Flow Chamber ◽  
Vapor Permeability ◽  
Intestinal Epithelial ◽  
Proof Of Concept ◽  
Epithelial Culture ◽  
Close Proximity ◽  
On Chip ◽  
Basal Flow

Here we report benchtop fabrication of multilayer thermoplastic organs-on-chips via laser cut and assembly of double sided adhesives. Biocompatibility was evaluated with Caco-2 cells and primary human intestinal organoids. Chips with Luer fluidic interfaces were economical ($2 per chip) and were fabricated in just hours without use of specialized bonding techniques. Compared with control static Transwell™ cultures, Caco-2 and organoids cultured on chips formed confluent monolayers expressing tight junctions with low permeability. Caco-2 cells on chip differentiated ∼4 times faster compared to controls and produced mucus. To demonstrate the robustness of laser cut and assembly, we fabricated a dual membrane, tri-layer gut chip integrating 2D monolayers, 3D cell culture, and a basal flow chamber. As proof of concept, we co-cultured a human, differentiated monolayer and intact organoids in a chip with multi-layered contacting compartments. The epithelium exhibited 3D tissue structure and organoids formed in close proximity to the adjacent monolayer. The favorable features of thermoplastics, such as low gas and water vapor permeability, in addition to rapid, facile, and economical fabrication of multilayered devices, make laser cut and assembly an ideal fabrication technique for developing organs-on-chips and studying multicellular tissues.

scholarly journals Microdroplet-based system for culturing of environmental microorganisms using FNAP-sort

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kanako Saito ◽  
Yuri Ota ◽  
Dieter M. Tourlousse ◽  
Satoko Matsukura ◽  
Hirotsugu Fujitani ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
High Purity ◽  
Droplet Microfluidics ◽  
Individual Growth ◽  
Proof Of Concept ◽  
Widespread Adoption ◽  
Technical Challenges ◽  
On Chip ◽  
Uncultured Microorganisms ◽  
Environmental Microbes

AbstractDroplet microfluidics has emerged as a powerful technology for improving the culturing efficiency of environmental microorganisms. However, its widespread adoption has been limited due to considerable technical challenges, especially related to identification and manipulation of individual growth-positive droplets. Here, we combined microfluidic droplet technology with on-chip “fluorescent nucleic acid probe in droplets for bacterial sorting” (FNAP-sort) for recovery of growth-positive droplets and droplet microdispensing to establish an end-to-end workflow for isolation and culturing of environmental microbes. As a proof-of-concept, we demonstrate the ability of our technique to yield high-purity cultures of rare microorganisms from a representative complex environmental microbiome. As our system employs off-the-shelf commercially available equipment, we believe that it can be readily adopted by others and may thus find widespread use toward culturing the high proportion of as-of-yet uncultured microorganisms in different biomes.

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