Influence of dietary intervention on microvascular endothelial function in coronary patients and atherothrombotic risk of recurrence

Endothelial dysfunction is a key player in both the onset and development of atherosclerosis. No study has examined whether healthy dietary patterns can improve microvascular endothelial function in patients with coronary heart disease (CHD) in the long-term and whether this relationship can affect patient’s risk of CHD recurrence. In the CORDIOPREV study, a randomized, double-blind, controlled trial, dietary intervention with either the Mediterranean diet or a low-fat diet was implemented in 1,002 CHD patients. A laser-doppler flowmetry was performed at baseline and after 6 years of follow up in 664 patients, evaluating the effects of this dietary intervention on microvascular basal flow and reactive hyperaemia area, as well as on the risk of CHD recurrence, based on the TRS2P risk score. Basal flow (97.78 ± 2.79 vs. 179.31 ± 5.06 arbitrary perfusion units, 83.38% increase, p < 0.001) and reactive hyperaemia area (4233.3 ± 127.73 vs. 9695.9 ± 205.23 arbitrary perfusion units per time, 129.04% increase, p < 0.001) improved after the dietary intervention in the cohort, without finding differences due to the diet (p > 0.05 for the diet-effect). When patients were stratified to low, moderate or high-risk of recurrence, basal flow was similarly increased in all three groups. However, reactive hyperaemia area was improved to a greater extent in patients at the low-risk group compared with those at moderate or high-risk. No differences were observed between diets. Healthy dietary patterns can improve microvascular endothelial function and this improvement persists in the long-term. Patients with a low-risk of CHD recurrence show a greater improvement in reactive vasodilation to ischemia than patients in the moderate or high-risk groups.

Identification of Gingival Microcirculation Using Laser Doppler Flowmetry in Patients with Orthodontic Treatment—A Longitudinal Pilot Study

Background and Objectives: Orthodontic tooth movement is associated with inflammatory responses. The aim of this study was to identify gingival microcirculation using laser Doppler flowmetry in patients with orthodontic treatment. Materials and Methods: A longitudinal pilot study was performed. The participants were selected using a non-probability consecutive sampling. Of the twenty-five subjects, a total of six (four women and two men) complied with the criteria. Before and during the treatment, the oral hygiene index, gingival index, probing depth, level of epithelial attachment, and gingival microcirculation were evaluated with laser Doppler flowmetry (integrated parameters: 1. integrated primary basal flow (IPBF), 2. integrated total secondary real flow (ITSRF), and 3. difference between integration (DBI)) in all of the participants). Results: (a) An increase in gingival blood flow was identified at all time intervals with different arches during orthodontic treatment. (b) The IPBF and ITSRF (with treatment) identified after 20 min (treatment initial stage) were compared with the different time intervals, and we observed an increase in gingival perfusion at the 24th, 48th, and 72nd hours in some arches. (b) In the DBI, we found statistically significant differences (p < 0.005) in the Nitinol group of 0.016 inches among all the time intervals (24 h, 48 h, and 72 h) within the 30-day interval, observing a flow increase three times greater than the basal flow after 30 days. Conclusions: Healthcare professionals must identify the inflammatory processes in treatment to observe and discontinue use of harmful methods in clinical practice.

Magmatic karst reveals dynamics of crystallization and differentiation in basaltic magma chambers

An understanding of magma chamber dynamics relies on answering three important yet highly controversial questions: where, why, and how magma chambers crystallize and differentiate. Here we report on a new natural phenomenon—the undercut-embayed chamber floor in the Bushveld Complex—which allows us to address these questions. The undercut-embayed floor is produced by magmatic karstification (i.e. erosion by dissolution) of the underlying cumulates by replenishing magmas that form basal flows on the chamber floor. This results in a few metres thick three-dimensional framework of spatially interconnected erosional remnants that separate the floor cumulates from the overlying resident melt. The basal flow in this environment is effectively cooled through the floor, inducing heterogeneous nucleation and in situ growth against much of its three-dimensional framework. The solidification front thus propagates in multiple directions from the surfaces of erosional remnants. Fractional crystallization may occur within this environment by convective removal of a compositional boundary layer from in situ growing crystals and is remarkably efficient even in very confined spaces. We propose that the way magma crystallizes and differentiates in the undercut-embayed chamber floor is likely common for the evolution of many basaltic magma chambers.

Experimental constraints on volcanic ash generation and clast morphometrics in pyroclastic density currents and granular flows

&lt;p&gt;Pyroclastic density currents (PDCs) present perhaps the greatest proximal primary hazard of volcanic activity and produce abundant fine ash that can present a range of health, environment and infrastructure hazards. However, direct, fully quantitative observation of ash production in PDCs is lacking, and little direct evidence exists to constrain the parameters controlling ash generation in PDCs. Here, we use an experimental approach to investigate the effects of starting mass, material density and ash removal on the efficiency of ash generation and concurrent clast rounding in the dense basal flow of PDCs. We employ a rotary drum to tumble pumice and scoria lapilli clasts over multiple transport &amp;#8220;distance&amp;#8221; steps (from 0.2 to 6&amp;#160;km). We observe increased ash generation rates with the periodic removal of ash during the experiments and with increasing starting mass. By scaling to the bed height and clast diameter we obtain a general description for ash production in all experiments as a function of flow distance, bed height and average clast diameter. We confirm that changes in lapilli shape factors correlate with the ash fraction generated and that the grain size of ash produced decreases with distance. Finally, we estimate shear rate in our experiments and calculate the inertial number, which describes the ratio between clast-scale and flow-scale rearrangement during flow. We show that, under certain conditions, fractional ash production can be calculated accurately for any starting mass solely as a function of the inertial number and the flow distance. This work sheds light on some of the first systematic and generalizable experimental parameterizations of ash production and associated clast evolution in PDCs and should advance our ability to understand flow mobility and associated hazards.&lt;/p&gt;

Experimental constraints on volcanic ash generation and clast morphometrics in pyroclastic density currents and granular flows

Pyroclastic density currents (PDCs) are a prominent hazard of volcanic activity; however, fully quantitative observations are lacking and little direct evidence exists to constrain the parameters controlling ash production and runout. Here, we use rotary tumbling experiments to investigate ash generation efficiency and clast morphometrics in the dense basal flow of PDCs. We observe greater ash generation with periodic ash removal and with higher starting mass. By scaling to the bed height and clast diameter we obtain a general description for ash production in all experiments as a function of flow distance that we parameterise in dimensionless space. We also show that ash production correlates with clast shape changes and with the Inertial number for our experiments. This work introduces some of the first systematic and generalizable experimental parameterizations of ash production and clast evolution in PDCs and should advance the ability to understand flow mobility and associated hazards.

The Effect of Muscle Exercise on Perforators Flow: A Prospective Cohort Study

Background and objectives: The metabolic response after exercise causes a significant increase in the muscle blood flow. While these effects are demonstrated for intra-muscular vessels, there is no evidence about the inter-muscular vessels, such as the septocutaneous perforators supplying the skin after they branch out from the deep source artery. The aim of our prospective study was to quantify the changes in the anterior tibial artery perforators arterial blood flow after mild isotonic exercise in a young and healthy population. Material and Methods: We performed a prospective analysis of 34 patients who were admitted to the Plastic Surgery Department from December 2019 to April 2020. Flow velocities of two previously identified anterior tibial artery perforators were recorded both before and after 10 complete flexion-extensions of the foot. The time to revert to basal flow was measured. We further classified the overmentioned patients based on their level of physical activity. Results: We registered a significant increase in systolic, diastolic and mean blood flow velocities both in proximal and distal anterior tibial artery perforators after exercise. Fitter patients exhibited a higher increase in proximal leg perforators than those who did less than three aerobic workouts a week. The time to return to basal flow ranged from 60 to 90 s. Conclusions: This was the first study to describe the effect of muscular activity on perforators blood flow. Even mild exercise significantly increases the perforator flow. Waiting at least two minutes at rest before performing the Doppler study, thus avoiding involved muscle activation, can notably improve the reliability of the pre-operative planning.

0600 Automated Oximetric Versus Standard Sleep Polygraphy Scoring

Introduction Agreement between automated standard respiratory event scoring and a novel, validated, and patented oximetry-based algorithm was assessed. Methods The standard apnea-hypopnea index (AHI) was derived by adding apneas (flow drop &gt;= 90% for 10 to 30 s) and hypopneas (flow drop &gt;= 30% for 10 to 60 s with oxygen saturation (SpO2) dropping &gt;=3%). The novel oxygen index (ODI4) was derived by scoring events where SpO2 dropped in each of three successive samples and cumulatively by &gt;= 4%. Agreement was assessed by Bland-Altman analysis Results AHI versus ODI4 and Bland-Altman plots showed a high prevalence of AHI &gt; ODI4 when AHI was&lt; 30/h. Negative difference outliers were frequent when mean index difference was &gt; 30/h. There was a bias of 2.83/h in the difference between AHI and ODI4 with upper and lower confidence limits of 22.0/h and -16.3/h. Conclusion Standard respiratory event scoring overestimates respiratory disturbance compared to a novel oximetric index. Standard automated scoring frequently over scores events when basal flow amplitude is low. Support None.

Rapid prototyping of a multilayer microphysiological system for primary human intestinal epithelial culture

Here we report benchtop fabrication of multilayer thermoplastic organs-on-chips via laser cut and assembly of double sided adhesives. Biocompatibility was evaluated with Caco-2 cells and primary human intestinal organoids. Chips with Luer fluidic interfaces were economical ($2 per chip) and were fabricated in just hours without use of specialized bonding techniques. Compared with control static Transwell™ cultures, Caco-2 and organoids cultured on chips formed confluent monolayers expressing tight junctions with low permeability. Caco-2 cells on chip differentiated ∼4 times faster compared to controls and produced mucus. To demonstrate the robustness of laser cut and assembly, we fabricated a dual membrane, tri-layer gut chip integrating 2D monolayers, 3D cell culture, and a basal flow chamber. As proof of concept, we co-cultured a human, differentiated monolayer and intact organoids in a chip with multi-layered contacting compartments. The epithelium exhibited 3D tissue structure and organoids formed in close proximity to the adjacent monolayer. The favorable features of thermoplastics, such as low gas and water vapor permeability, in addition to rapid, facile, and economical fabrication of multilayered devices, make laser cut and assembly an ideal fabrication technique for developing organs-on-chips and studying multicellular tissues.